British Journal of Biomedical Science最新文献_第9页

Endoscopic management of acute oesophageal variceal bleeding within 12 hours of admission is superior to 12-24 hours. 内镜下治疗急性食管静脉曲张出血在入院12小时内优于12-24小时。

IF 1.9 4区医学

British Journal of Biomedical Science Pub Date : 2021-07-01 Epub Date: 2021-03-17 DOI: 10.1080/09674845.2020.1857049

N Mousa, A Abdel-Razik, T Sheta, A G Deiab, A Habib, M Diasty, A Eldesoky, A Taha, E Mousa, A Yassen, A Fathy, A Elgamal

{"title":"Endoscopic management of acute oesophageal variceal bleeding within 12 hours of admission is superior to 12-24 hours.","authors":"N Mousa, A Abdel-Razik, T Sheta, A G Deiab, A Habib, M Diasty, A Eldesoky, A Taha, E Mousa, A Yassen, A Fathy, A Elgamal","doi":"10.1080/09674845.2020.1857049","DOIUrl":"https://doi.org/10.1080/09674845.2020.1857049","url":null,"abstract":"Background: Acute oesophageal variceal haemorrhage (AOVH) is a medical emergency. The American Association for the Study of Liver Diseases recommends endoscopy management as soon as possible and not more than 12 hours after presentation. The United Kingdom guidelines recommended endoscopy for unstable patients with severe acute upper gastrointestinal bleeding immediately after resuscitation and within 24 hours of admission. We aimed to evaluate the outcome of endoscopic management of AOVH in less than 12 hours compared to 12-24 hours post admission.Methods: 297 patients with AOVH were divided into groups depending on the timing of the endoscopic management: 180 within 12 h of admission and 117 patients at 12-24 hours of admission. Routine clinical and laboratory data were collected.Results: Compared to patients with endoscopic management at 12-24 hours (mean 16 hours), patients with endoscopic management within 12 hours (mean 8.3 hours) of admission had fewer hospital stay days (P = 0.001), significant reduction of ammonia levels (P < 0.0001) and significant improvement in associated hepatic encephalopathy grade 25 (p = 0.048). There were no major clinical events in the 12-hour group, but 8 events in the 12-24 hour group (p < 0.01).Conclusion: Endoscopic management of acute variceal bleeding within 12 hours of admission is superior to endoscopic management at 12-24 hours of admission regarding reduction of hospital stay, ammonia levels, correction of hepatic encephalopathy, re-bleeding and mortality rate, hence, reducing the cost of treatment benefiting patient satisfaction and improving hospital bed availability.","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"78 3","pages":"130-134"},"PeriodicalIF":1.9,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1857049","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38699274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Interaction between lncRNAs HOTAIR and MALAT1 tagSNPs in gastric cancer. lncRNAs HOTAIR和MALAT1标签snp在胃癌中的相互作用

IF 1.9 4区医学

British Journal of Biomedical Science Pub Date : 2021-07-01 Epub Date: 2021-04-30 DOI: 10.1080/09674845.2020.1866260

E Abdi, S Latifi-Navid, V Kholghi-Oskooei, F Pourfarzi, A Yazdanbod

引用次数: 13

Evaluation of different haematoxylin stain subtypes for the optimal microscopic interpretation of cutaneous malignancy in Mohs frozen section histological procedure. 莫氏冷冻切片组织学检查中不同血红素染色亚型对皮肤恶性肿瘤最佳显微解释的评价。

IF 1.9 4区医学

British Journal of Biomedical Science Pub Date : 2021-04-01 Epub Date: 2020-12-04 DOI: 10.1080/09674845.2020.1838075

J A Gabriel, M Shams, G E Orchard

{"title":"Evaluation of different haematoxylin stain subtypes for the optimal microscopic interpretation of cutaneous malignancy in Mohs frozen section histological procedure.","authors":"J A Gabriel, M Shams, G E Orchard","doi":"10.1080/09674845.2020.1838075","DOIUrl":"https://doi.org/10.1080/09674845.2020.1838075","url":null,"abstract":"Background: The Mohs technique employs mainly H&E-stained frozen sections for surgical margin assessment of cutaneous excisions, utilising microscopic evaluation of the complete, circumferential, peripheral and deep margins. This study aimed to determine which mordant based haematoxylin (Ehrlich's, Cole's, Mayer's, Gill's I, Gill's II, Gill's III, Weigert's, Harris' or Carazzi's) produced the optimal morphological clarity of staining for the identification of cellular and tissue morphology of cutaneous basal cell carcinoma (BCC).Material and methods: In total, 100 anonymised patient cases were selected, sectioned and stained with each haematoxylin subtype. The slides were independently evaluated microscopically by two assessors. A combined score was generated to determine the sensitivity (defined as the intensity of haematoxylin staining being too weak or too strong and the colour appearance of the haematoxylin not being blue/black) and specificity (defined as the appearance of background staining with haematoxylin, uneven staining and staining deposits) for each of the nine haematoxylin subtypes. The scoring criteria were based on the UKNEQAS CPT Mohs procedure assessment criteria.Results: The scores generated for specificity identified Carazzi's haematoxylin as best performing (99.2%) followed by Gill's III (98.4%), Ehrlich's (98.2%) and Harris' (85.0%). The sensitivity score again identified Carazzi's as producing the best result (85.0%) followed by Weigert's (83.4%), Ehrlich's (81.6%) and Gill's III (80.4%).Discussion: Carazzi's haematoxylin is the most optimal staining dye for the identification of BCC tumour for use as part of the Mohs micrographic surgery procedure.","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"78 2","pages":"78-86"},"PeriodicalIF":1.9,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1838075","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38589468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Long non-coding RNA ANRIL polymorphisms in papillary thyroid cancer and its severity. 长链非编码RNA ANRIL在甲状腺乳头状癌中的多态性及其严重程度。

IF 1.9 4区医学

British Journal of Biomedical Science Pub Date : 2021-04-01 Epub Date: 2021-01-20 DOI: 10.1080/09674845.2020.1829853

R Maruei-Milan, Z Heidari, A Aryan, M Asadi-Tarani, S Salimi

{"title":"Long non-coding RNA ANRIL polymorphisms in papillary thyroid cancer and its severity.","authors":"R Maruei-Milan, Z Heidari, A Aryan, M Asadi-Tarani, S Salimi","doi":"10.1080/09674845.2020.1829853","DOIUrl":"https://doi.org/10.1080/09674845.2020.1829853","url":null,"abstract":"Background: Long non-coding RNAs are likely to have a role in the pathogenesis of many diseases, including cancer. We hypothesised an effect of certain ANRIL single nucleotide polymorphisms (SNPs) in papillary thyroid cancer. Methods: Genomic ANRIL SNPs in rs11333048, rs4977574, rs1333040 and rs10757274 were determined in 134 papillary thyroid cancer patients and 155 age- and sex-matched controls. Results: None of the ANRIL SNPs were individually linked to papillary thyroid cancer. However, the AAAC haplotype (A from rs11333048, A from rs4977574, A from rs1333040 and C from rs10757274, respectively) showed a protective effect from papillary thyroid cancer whilst the CAAC and CAGT haplotypes were associated with cancer. The rs1333048 CC variant was more frequent in patients with larger tumour size (≥1 cm) in a recessive model (OR 3.4 [95%CI, 1.1-11], P = 0.035). The rs4977574 AC variant was associated with smaller tumour size in an over-dominant model (OR 0.4 [95%CI, 0.2-1.0], P = 0.041). SNPs in rs10757274 (AA: p = 0.045) and rs1333040 (CC: p = 0.019) are linked to a lower likelihood of III-IV cancer stages in dominant or codominant models. Conclusions: Certain haplotypes of ANRIL SNPs are associated with papillary thyroid cancer. ANRIL rs1333048 and rs4977574 variants were associated with larger and smaller tumour sizes, respectively. rs10757274 and rs1333040 variants might lead to lower III-IV cancer stages. These SNPs may be important in the diagnosis of this form of thyroid cancer.","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"78 2","pages":"58-62"},"PeriodicalIF":1.9,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1829853","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38601822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

HCC-Mark: a simple non-invasive model based on routine parameters for predicting hepatitis C virus related hepatocellular carcinoma. HCC-Mark:一种基于常规参数预测丙型肝炎病毒相关肝细胞癌的简单无创模型

IF 1.9 4区医学

British Journal of Biomedical Science Pub Date : 2021-04-01 Epub Date: 2020-11-18 DOI: 10.1080/09674845.2020.1832371

M Omar, K Farid, T Emran, F El-Taweel, A Tabll, M Omran

{"title":"HCC-Mark: a simple non-invasive model based on routine parameters for predicting hepatitis C virus related hepatocellular carcinoma.","authors":"M Omar, K Farid, T Emran, F El-Taweel, A Tabll, M Omran","doi":"10.1080/09674845.2020.1832371","DOIUrl":"https://doi.org/10.1080/09674845.2020.1832371","url":null,"abstract":"Background: Early detection of hepatocellular carcinoma (HCC) is crucial in providing more effective therapies. As routine laboratory variables are readily accessible, this study aimed to develop a simple non-invasive model for predicting hepatocellular cancer.Methods: Two groups of patients were recruited: an estimation group (n = 300) and a validation group (n = 625). Each comprised two categories: hepatocellular cancer and liver cirrhosis. Logistic regression analyses and receiver operating characteristic (ROC) curves were used to develop and validate the HCC-Mark model comprising AFP, high-sensitivity C-reactive protein, albumin and platelet count. This model was tested in cancer patients classified by the Barcelona Clinic Liver Cancer (BCLC), Cancer of Liver Italian Program (CLIP) and Okuda systems, and was compared with other non-invasive models for predicting hepatocellular cancer.Results: HCC-Mark produced a ROC AUC of 0.89 (95% CI 0.85-0.90) for discriminating hepatocellular carcinoma from liver cirrhosis in the estimation group and 0.90 (0.86-0.90) in the validation group (both p < 0.0001). This AUC exceeded all other models, that had AUCs from 0.41 to 0.81. AUCs of HCC-Mark for discriminating patients with a single focal lesion, absent macrovascular invasion, tumour size <2 cm, BCLC (0-A), CLIP (0-1) and Okuda (stage Ι) from cirrhotic patients were 0.88 (0.85-0.90), 0.87 (0.85-0.89), 0.89 (0.85-0.93), 0.87 (0.84-0.89), 0.85 (0.82-0.87) and 0.86 (0.83-0.89), respectively (all p < 0.0001).Conclusion: HCC-Mark is an accurate and validated model for the detection of hepatocellular cancer and certain of its clinical features.","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"78 2","pages":"72-77"},"PeriodicalIF":1.9,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1832371","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38555952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Association of miR-146a rs57095329 with Behçet's disease and its complications. miR-146a rs57095329与behet病及其并发症的关系

IF 1.9 4区医学

British Journal of Biomedical Science Pub Date : 2021-04-01 Epub Date: 2020-08-06 DOI: 10.1080/09674845.2020.1786284

O O Abdelaleem, N A Fouad, O G Shaker, H A Hussein, F A Ahmed, D Y Ali, H S Elsayed

{"title":"Association of miR-146a rs57095329 with Behçet's disease and its complications.","authors":"O O Abdelaleem, N A Fouad, O G Shaker, H A Hussein, F A Ahmed, D Y Ali, H S Elsayed","doi":"10.1080/09674845.2020.1786284","DOIUrl":"https://doi.org/10.1080/09674845.2020.1786284","url":null,"abstract":"Background: Behçet's disease is a chronic relapsing and remitting autoimmune multisystem inflammatory disease characterised by oral aphthae, genital ulcers, skin lesions, gastrointestinal involvement, arthritis, vascular lesions and neurological manifestations. We hypothesised a link between rs57095329 of miR-146a and Behçet's disease, with further links with common clinical features.Methods: We tested our hypothesis in 130 Behçet's disease patients and 131 age and sex-matched healthy controls. Behcet's disease current activity index (BDCAI) was used to assess patients' disease activity status. MiR-146a (rs57095329) was genotyped in all participants using RT-PCR and results in patients analysed according to clinical features.Results: The frequency of the GG and AG genotypes in rs57095329 were strongly associated with Behçet's disease (adjusted OR 8.05, 95% CI 3.63-17.82; P < 0.001 and OR 2.26, 95% CI 1.27-4.04; P = 0.006, respectively), and in dominant (GG+AG > AA) and recessive (GG > AA+AG) models (both P < 0.001). Additionally, G allele distribution was significantly greater in Behçet's disease compared with controls (OR 2.85, 95% CI 1.98-4.11, P < 0.001). The AA genotype and A allele were linked to oral ulcers, the GG genotype and G allele to neurological disease, and the GG genotype and G allele to ocular disease (all P < 0.01). There were no links with genital ulceration, skin lesions, vascular disease or the result of the pathergy test.Conclusion: The miR-146a (rs57095329) is associated with Behçet's disease and certain genotypes and alleles with oral ulcers, and with ocular and neurological manifestations.","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"78 2","pages":"63-66"},"PeriodicalIF":1.9,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1786284","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38075917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Certain haplotypes of the 3'-UTR region of the HLA-G gene are linked to breast cancer. HLA-G基因的3'-UTR区域的某些单倍型与乳腺癌有关。

IF 1.9 4区医学

British Journal of Biomedical Science Pub Date : 2021-04-01 Epub Date: 2021-01-18 DOI: 10.1080/09674845.2020.1856495

M Haghi, M Ranjbar, K Karari, S Samadi-Miandoab, A Eftekhari, M A Hosseinpour-Feizi

{"title":"Certain haplotypes of the 3'-UTR region of the HLA-G gene are linked to breast cancer.","authors":"M Haghi, M Ranjbar, K Karari, S Samadi-Miandoab, A Eftekhari, M A Hosseinpour-Feizi","doi":"10.1080/09674845.2020.1856495","DOIUrl":"https://doi.org/10.1080/09674845.2020.1856495","url":null,"abstract":"Background: Human leukocyte antigen G belongs to the family of non-classical HLA class I genes, its expression considered an important immune escape mechanism of cancer cells. The polymorphisms in the 3'-untranslated region (UTR) region of HLA-G influence the magnitude of the protein by modulating HLA-G mRNA stability. We hypothesised links between any of eight (UTR) single nucleotide polymorphisms (SNPs) and their haplotype of the HLA-G gene with breast cancer. Materials and Methods: Peripheral blood DNA from 100 patients affected by breast cancer and 100 controls was PCR sequenced for genotyping of 25 HLA-G 3'-UTR regions, including rs371194629 (+2960), rs1707 (+3003), rs1710 (+3010), rs17179101 (+3027), rs1063320 (+3142), rs9380142 (+3187), rs1610696 (+3196), and rs1233331 (+3227). Results: The 14-bp deletion (p = 0.01), and the +3010 (p = 0.021), +3142 (p = 0.006) and +3187 (p = 0.046) variants were significantly more prevalent in patients than in controls. In combining these data, two haplotypes of all eight SNPs and deletion/insertion (UTR-1 and UTR-4) are associated with breast cancer. Conclusion: Certain variants in the 3-UTR, and their combination as a haplotype, of the HLA-G gene are linked to breast cancer.","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"78 2","pages":"87-91"},"PeriodicalIF":1.9,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1856495","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38641175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Pseudomonas aeruginosa - Candida interplay: effect on in vitro antibiotic susceptibility of Pseudomonas aeruginosa when grown in the presence of Candida culture. 铜绿假单胞菌与念珠菌的相互作用:在念珠菌培养中生长对铜绿假单胞菌体外抗生素敏感性的影响。

IF 1.9 4区医学

British Journal of Biomedical Science Pub Date : 2021-04-01 Epub Date: 2020-09-23 DOI: 10.1080/09674845.2020.1819003

R McIlroy, B C Millar, D W Nelson, A Murphy, J R Rao, D G Downey, J E Moore

引用次数: 1

KISS1R polymorphism rs587777844 (Tyr313His) is linked to female infertility. KISS1R多态性rs587777844 (Tyr313His)与女性不孕有关。

IF 1.9 4区医学

British Journal of Biomedical Science Pub Date : 2021-04-01 Epub Date: 2021-01-18 DOI: 10.1080/09674845.2020.1856496

Z Siahpoosh, M Farsimadan, M Pazhohan, H Vaziri, M Mahmoudi Gomari

引用次数: 17

Overexpression of long non-coding RNA MCM3AP-AS1 in breast cancer tissues compared to adjacent non-tumour tissues. 与邻近非肿瘤组织相比，长链非编码RNA MCM3AP-AS1在乳腺癌组织中的过表达

IF 1.9 4区医学

British Journal of Biomedical Science Pub Date : 2021-04-01 Epub Date: 2020-09-07 DOI: 10.1080/09674845.2020.1798058

A Riahi, M Hosseinpour-Feizi, A Rajabi, M Akbarzadeh, V Montazeri, R Safaralizadeh

{"title":"Overexpression of long non-coding RNA MCM3AP-AS1 in breast cancer tissues compared to adjacent non-tumour tissues.","authors":"A Riahi, M Hosseinpour-Feizi, A Rajabi, M Akbarzadeh, V Montazeri, R Safaralizadeh","doi":"10.1080/09674845.2020.1798058","DOIUrl":"https://doi.org/10.1080/09674845.2020.1798058","url":null,"abstract":"Background: Altered expression of several long non-coding RNAs (lncRNAs) has been described in numerous malignancies, including breast cancer, and some may have a role in carcinogenesis. We hypothesised differences in the expression of lncRNA MCM3AP-AS1 in breast cancer tissues compared to nearby healthy tissues and potential links with clinical features.Methods: We tested our hypothesis in 102 pairs of breast cancer tumours and adjacent non-tumour tissues from female patients. After RNA extraction, cDNA synthesis was performed for all specimens. The differential gene expression was assessed using Quantitative Real-Time PCR Technique.Results: There was a significant overexpression of the lncRNAs in tumour tissues as compared with their adjacent non-tumour tissues (P < 0.001). Expression was significantly linked with the tumour oestrogen receptor expression (P = 0.023) and tumour progesterone receptor expression (P < 0.001). ROC analysis showed an AUC of 0.67 (95% CI 0.60-0.75) (P < 0.001) with sensitivity and specificity of 58% and 76%, respectively.Conclusion: The lncRNA MCM3AP-AS1 may be a novel breast cancer lncRNA with high expression levels in breast cancer patients' tissue. Further investigations are needed to confirm its uses as a potential molecular marker and therapeutic target.","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"78 2","pages":"53-57"},"PeriodicalIF":1.9,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1798058","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38163992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13