MicroRNA Variants miR-27a rs895819 and miR-423 rs6505162, but not miR-124-1 rs531564, are Linked to Endometriosis and its Severity.

IF 2.7 4区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

British Journal of Biomedical Science Pub Date : 2022-01-07 eCollection Date: 2022-01-01 DOI:10.3389/bjbs.2021.10207

S O Jaafar, J O Jaffar, S A Ibrahim, K K Jarjees

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引用次数: 4

Abstract

Background: While different studies have investigated the association of SNPs with female reproductive disorders, a limited number of studies have investigated the effect of microRNAs variants in endometriosis. In this study, we evaluated the prevalence and the association of three different miRNAs variants including, miR-27a rs895819, miR-124-1 rs531564, and miR-423 rs6505162 with endometriosis to help further elucidate the importance of these variants in female reproductive disorders. Methods: A total number of 440 women (220 cases and 220 controls) were included. DNA was extracted and genotyping of the SNPs was carried out by PCR. Results: The results showed that rs895819 and rs6505162 had a significant association with endometriosis under the dominant, recessive, co-dominant, and allelic model, but rs531564 was not linked to endometriosis. Our results also imply a protective effect on endometriosis severity for AG genotype and G allele in rs895819 (p < 0.001), and also for AA and AC genotypes in rs6505162 with severity in endometriosis (p < 0.001). Moreover, Hardy-Weinberg equilibrium, haplotype frequency, and linkage disequilibrium between SNPs were performed. Conclusion: miR-27a rs895819 and miR-423 rs6505162, but not miR-124-1 rs531564, are linked to endometriosis.

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与子宫内膜异位症及其严重程度相关的MicroRNA变体miR-27a rs895819和miR-423 rs6505162，而不是miR-124-1 rss531564。

背景:虽然不同的研究调查了snp与女性生殖障碍的关系，但有限数量的研究调查了microRNAs变异在子宫内膜异位症中的作用。在本研究中，我们评估了miR-27a rs895819、miR-124-1 rss531564和miR-423 rs6505162三种不同miRNAs变体与子宫内膜异位症的患病率和相关性，以帮助进一步阐明这些变体在女性生殖障碍中的重要性。方法:共纳入440例妇女(220例，对照组220例)。提取DNA，用PCR对snp进行基因分型。结果:结果显示rs895819和rs6505162在显性、隐性、共显性和等位基因模型下与子宫内膜异位症有显著相关性，而rss531564与子宫内膜异位症无相关性。我们的研究结果还表明，rs895819的AG基因型和G等位基因对子宫内膜异位症的严重程度有保护作用(p < 0.001)， rs6505162的AA和AC基因型对子宫内膜异位症的严重程度也有保护作用(p < 0.001)。此外，还进行了Hardy-Weinberg平衡、单倍型频率和snp之间的连锁不平衡。结论:miR-27a rs895819和miR-423 rs6505162与子宫内膜异位症有关，而miR-124-1 rss531564与子宫内膜异位症无关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

British Journal of Biomedical Science 医学-医学实验技术

CiteScore

4.40

自引率

15.80%

发文量

审稿时长

>12 weeks

期刊介绍： The British Journal of Biomedical Science is committed to publishing high quality original research that represents a clear advance in the practice of biomedical science, and reviews that summarise recent advances in the field of biomedical science. The overall aim of the Journal is to provide a platform for the dissemination of new and innovative information on the diagnosis and management of disease that is valuable to the practicing laboratory scientist.