British Journal of Biomedical Science最新文献

{"title":"<i>ACE2</i> and <i>TMPRSS2</i> SNPs as Determinants of Susceptibility to, and Severity of, a COVID-19 Infection.","authors":"S Abdelsattar, Z A Kasemy, S F Ewida, R A A Abo-Elsoud, A A Zytoon, G A Abdelaal, A S Abdelgawad, F O Khalil, H F M Kamel","doi":"10.3389/bjbs.2021.10238","DOIUrl":"10.3389/bjbs.2021.10238","url":null,"abstract":"<p><p><b>Background:</b> Genetic risk factors may be related to the infectivity and severity of SARS-CoV-2 infection. Angiotensin-converting enzyme 2 (ACE2) and host transmembrane serine protease (TMPRSS2) have key role in viral cell entrance and priming. <b>Methods:</b> This case-control study on 147 healthy controls and 299 COVID-19 patients identified potential determinants and risk factors, including gene polymorphism involved in the severity (mild, moderate, severe) of COVID-19 disease defined by CORAD radiological criteria. <b>Results:</b> The ACE2 s2285666 and TMPRSS2 rs12329760 SNPs were significantly linked with COVID-19 disease severity, as were certain co-morbidities (hypertension, heart disease) and laboratory parameters. Both SNPs were amongst the highest predictors of disease severity: TMPRSS2 rs12329760 CT + TT [odds ratio (95% CI) 17.6 (5.1-61.10), ACE2 rs2285666 CT + TT 9.9 (3.2-30.9), both <i>p</i> < 0.001]. There was an increase in the expression of genotype frequencies of ACE2 rs2285666 and TMPRSS2 rs1232976 (TT), (CT + TT), and (T) allele in severe COVID-19 group compared to control and mild groups. Disease severity was also linked to elevated CRP, ferritin and D-dimer, and lower lymphocytes and platelet count (all <i>p</i> < 0.001). <b>Conclusion:</b> ACE2 rs2285666 and TMPRSS2 rs12329760 SNPs, in addition to lymphocyte count, CRP, D-dimers, ferritin, and hypertension, are predictors of COVID-19 disease severity.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":null,"pages":null},"PeriodicalIF":2.7,"publicationDate":"2022-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8915702/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40435897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Significance of PEAK1 Expression and BRAF V600E Mutation in Papillary Thyroid Cancer.","authors":"P Li,&nbsp;H Zhao,&nbsp;X Liu,&nbsp;Y Huang,&nbsp;D Chen","doi":"10.3389/bjbs.2021.10268","DOIUrl":"https://doi.org/10.3389/bjbs.2021.10268","url":null,"abstract":"Department of Endocrinology, The Affiliated Hospital of Qingdao University, Qingdao, China, Department of Pathology, The Affiliated Hospital of Qingdao University, Qingdao, China, Department of Nuclear Medicine, The Affiliated Hospital of Qingdao University, Qingdao, China, Department of Otorhinolaryngology, The Affiliated Hospital of Qingdao University, Qingdao, China, Department of General Surgery, The Affiliated Hospital of Qingdao University, Qingdao, China","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8915583/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40631253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Diagnostics of Ciliopathies and Insights Into Novel Developments in Diagnosing Rare Diseases.","authors":"K Modarage,&nbsp;S A Malik,&nbsp;P Goggolidou","doi":"10.3389/bjbs.2021.10221","DOIUrl":"https://doi.org/10.3389/bjbs.2021.10221","url":null,"abstract":"<p><p>The definition of a rare disease in the European Union describes genetic disorders that affect less than 1 in 2,000 people per individual disease; collectively these numbers amount to millions of individuals globally, who usually manifest a rare disease early on in life. At present, there are at least 8,000 known rare conditions, of which only some are clearly molecularly defined. Over the recent years, the use of genetic diagnosis is gaining ground into informing clinical practice, particularly in the field of rare diseases, where diagnosis is difficult. To demonstrate the complexity of genetic diagnosis for rare diseases, we focus on Ciliopathies as an example of a group of rare diseases where an accurate diagnosis has proven a challenge and novel practices driven by scientists are needed to help bridge the gap between clinical and molecular diagnosis. Current diagnostic difficulties lie with the vast multitude of genes associated with Ciliopathies and trouble in distinguishing between Ciliopathies presenting with similar phenotypes. Moreover, Ciliopathies such as Autosomal Recessive Polycystic Kidney Disease (ARPKD) and Meckel-Gruber syndrome (MKS) present with early phenotypes and may require the analysis of samples from foetuses with a suspected Ciliopathy. Advancements in Next Generation Sequencing (NGS) have now enabled assessing a larger number of target genes, to ensure an accurate diagnosis. The aim of this review is to provide an overview of current diagnostic techniques relevant to Ciliopathies and discuss the applications and limitations associated with these techniques.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8915726/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40435896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Value of Bile Acids in Diagnosing Hepatitis C Virus-Induced Liver Cirrhosis and Hepatocellular Carcinoma.","authors":"Ashraf Khalil,&nbsp;Azza ElSheashaey,&nbsp;Eman Abdelsameea,&nbsp;Manar Obada,&nbsp;Mohamed Bayomy F F,&nbsp;Hala El-Said","doi":"10.3389/bjbs.2021.10191","DOIUrl":"https://doi.org/10.3389/bjbs.2021.10191","url":null,"abstract":"<p><p><b>Background:</b> Metabonomic studies have related bile acids to hepatic impairment, but their role in predicting hepatocellular carcinoma still unclear. The study aimed to examine the feasibility of bile acids in distinguishing hepatocellular carcinoma from post hepatitis C virus-induced liver cirrhosis. <b>Methods:</b> An ultra-performance liquid chromatography coupled with mass spectrometry measured 14 bile acids in patients with noncirrhotic post hepatitis C virus disease (n = 50), cirrhotic post hepatitis C virus disease (n = 50), hepatocellular carcinoma (n = 50), and control group (n = 50). <b>Results:</b> The spectrum of liver disease was associated with a significant increase in many conjugated bile acids. The fold changes in many bile acid concentrations showed a linear trend with hepatocellular carcinoma > cirrhotic disease > noncirrhotic disease > healthy controls (<i>p</i> < 0.05). Receiver operating characteristic curve analysis revealed five conjugated acids TCA, GCA, GUDCA, TCDCA, GCDCA, that discriminated hepatocellular carcinoma from noncirrhotic liver patients (AUC = 0.85-0.96) with a weaker potential to distinguish it from chronic liver cirrhosis (AUC = 0.41-0.64). <b>Conclusion:</b> Serum bile acids are associated primarily with liver cirrhosis with little value in predicting the progress of cirrhotic disease to hepatocellular carcinoma.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8915635/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40435900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNA Variants miR-27a rs895819 and miR-423 rs6505162, but not miR-124-1 rs531564, are Linked to Endometriosis and its Severity.","authors":"S O Jaafar,&nbsp;J O Jaffar,&nbsp;S A Ibrahim,&nbsp;K K Jarjees","doi":"10.3389/bjbs.2021.10207","DOIUrl":"https://doi.org/10.3389/bjbs.2021.10207","url":null,"abstract":"<p><p><b>Background:</b> While different studies have investigated the association of SNPs with female reproductive disorders, a limited number of studies have investigated the effect of microRNAs variants in endometriosis. In this study, we evaluated the prevalence and the association of three different miRNAs variants including, miR-27a rs895819, miR-124-1 rs531564, and miR-423 rs6505162 with endometriosis to help further elucidate the importance of these variants in female reproductive disorders. <b>Methods:</b> A total number of 440 women (220 cases and 220 controls) were included. DNA was extracted and genotyping of the SNPs was carried out by PCR. <b>Results:</b> The results showed that rs895819 and rs6505162 had a significant association with endometriosis under the dominant, recessive, co-dominant, and allelic model, but rs531564 was not linked to endometriosis. Our results also imply a protective effect on endometriosis severity for AG genotype and G allele in rs895819 (<i>p</i> < 0.001), and also for AA and AC genotypes in rs6505162 with severity in endometriosis (<i>p</i> < 0.001). Moreover, Hardy-Weinberg equilibrium, haplotype frequency, and linkage disequilibrium between SNPs were performed. <b>Conclusion:</b> miR-27a rs895819 and miR-423 rs6505162, but not miR-124-1 rs531564, are linked to endometriosis.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8915672/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40435899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Variants miR-126, miR-146a, miR-196a2, and miR-499 in Polycystic Ovary Syndrome.","authors":"R Li,&nbsp;Y Yu,&nbsp;S O Jaafar,&nbsp;B Baghchi,&nbsp;M Farsimadan,&nbsp;I Arabipour,&nbsp;H Vaziri","doi":"10.3389/bjbs.2021.10209","DOIUrl":"https://doi.org/10.3389/bjbs.2021.10209","url":null,"abstract":"<p><p><b>Introduction:</b> Alterations in certain microRNAs (miRNAs) and their target genes have reported in polycystic ovary syndrome (PCOS) and other disease of the female reproductive system, and so may be potential biomarkers. We hypothesised alterations in the prevalence of four miRNAs single nucleotide polymorphism (SNP) variants miR-126 rs4636297, miR-146a rs2910164, miR-196a2 rs11614913, and miR-499 rs3746444 in women with PCOS in comparison to healthy controls. <b>Methods:</b> SNPs in the four miRNAs were determined in 385 patients and 385 controls by standard RT-PCR techniques. <b>Results:</b> SNPs in miR-126 and miR-246a were significant linked with PCOS under the allelic, dominant, co-dominant, and recessive models (all <i>p</i> ≤ 0.01). The SNP in miR-499 was linked to PCOS in allelic (T, <i>p</i> = 0.002), dominant (<i>p</i> = 0.035) and recessive (<i>p</i> = 0.003) models. The SNPs -196a was significant linked to PCOS only in the recessive model (<i>p</i> = 0.037). Combining these SNPs in miR-499, mi146a, miR-196a and miR-126 respectively into allele haplotypes found highly significant odds ratios (95% CI) of 0.40 (0.29-0.54) (<i>p</i> < 0.001) for the C-G-C-G haplotype, and 0.46 (0.30-0.70) (<i>p</i> = 0.002) for the C-C-C-A haplotype (<i>p</i> = 0.002) for PCOS. <b>Conclusion:</b> Single SNPs and haplotype combinations in certain SNPs in miR-126, miR-146a, miR-196a2 and miR-499 are strongly linked to PCOS, and so may be useful predictors of this condition.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8915673/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40632709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intermittent Short-Duration Re-oxygenation Attenuates Cardiac Changes in Response to Hypoxia: Histological, Ultrastructural and Oxidant/Antioxidant Parameters.","authors":"Ayed A Shati,&nbsp;Mohamed Samir A Zaki,&nbsp;Youssef A Alqahtani,&nbsp;Mohamed A Haidara,&nbsp;Mohammed A Alshehri,&nbsp;Amal F Dawood,&nbsp;Refaat A Eid","doi":"10.3389/bjbs.2022.10150","DOIUrl":"https://doi.org/10.3389/bjbs.2022.10150","url":null,"abstract":"<p><p><b>Context:</b> Intermittent short-duration re-oxygenation attenuates cardiac changes in response to hypoxia. <b>Objective:</b> To see if intermittent short-duration re-oxygenation may protect the heart muscle from hypoxia damage. <b>Materials and Methods:</b> Eighteen albino rats were used to carry out the study. Rats divided into: (normoxia); rats exposed to room air as a control, second (hypoxic) group; rats subjected to a pressure of 405 mmHg in a hypobaric chamber to simulate hypoxia at 5,000 m, and third (intermittent short-duration re-oxygenation); rats exposed to room air three times per day. Experiments were all 14 days long. <b>Results:</b> Hypoxia enhanced the oxidative stress biomarker malondialdehyde while lowering the antioxidant superoxide dismutase . The levels of tumour necrosis factor (TNF-α) and interleukin-6 (IL-6) in the myocardium were elevated in hypoxic hearts. The hypoxic rats' cardiac myofibrils showed disarray of muscle fibres, vacuolation of the sarcoplasm, pyknosis of the nucleus, and expansion of intercellular gaps on histological examination. In addition, cardiomyocytes showed degenerative defects in ventricular myocardial cells on ultrastructural analysis. Myofibril thinning and degenerative mitochondrial changes affected intercalated discs with fascia adherent, desmosomes, and gap junction. Intermittent short-duration re-oxygenation improve cardiac histological, ultrastructural and oxidant/antioxidant parameters changes during hypoxia. <b>Conclusion:</b> Hypoxia showed a substantial impact on myocardial architecture, as well as increased oxidative stress and pro-inflammatory cytokines. Intermittent short-duration re-oxygenation significantly decreases hypoxia-induced cardiac changes.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302540/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9828773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leukocytosis induced by tigecycline in two patients with severe acute pancreatitis.","authors":"X Li,&nbsp;L Li,&nbsp;T Liu,&nbsp;X Hai,&nbsp;B Sun","doi":"10.1080/09674845.2021.1885865","DOIUrl":"https://doi.org/10.1080/09674845.2021.1885865","url":null,"abstract":"Department of Pharmacy, The First Affiliated Hospital of Harbin Medical University, Harbin, China; Department of Pancreatic and Biliary Surgery, The First Affiliated Hospital of Harbin Medical University; Key Laboratory of Hepatosplenic Surgery, Ministry of Education, The First Affiliated Hospital of Harbin Medical University, Harbin, China; Department of Pharmacy, Harbin Medical University Cancer Hospital, Harbin, China","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2021.1885865","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25378830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increased serum CA125 II, but not CEA，CA19-9，AFP or CA72-4 in colon cancer compared to rectal cancer.","authors":"T Liu,&nbsp;X Li,&nbsp;D Liu,&nbsp;S Liu,&nbsp;M Dong","doi":"10.1080/09674845.2020.1868685","DOIUrl":"https://doi.org/10.1080/09674845.2020.1868685","url":null,"abstract":"Globally, colorectal cancer is the third most frequent cancer type, with >1.4 million new cases and >690,000 deaths annually [1]. Survival from colorectal cancer is significantly dependent on the stage at diagnosis, with the 5-year rate at ~90% for localized disease, 70% for regional disease and 13% for distantly metastatic disease [2]. Several screening tests, including faecal occult blood test and colonoscopy, are frequently used in the detection of colorectal cancer. However, none are established and well-accepted screening tools due to their invasiveness, high cost or low sensitivity [3]. Therefore, the search for more sensitive, easily detected and representative biomarkers is of great significance for the early diagnosis and monitoring of this disease. Several biological and clinical hallmarks indicate that rectal cancer is different from colon cancer. The rectum and colon have a different embryological origin, anatomy and function [4]. Consequently, the treatments for primary rectal and colon cancer are different. Primary rectal cancer requires specific surgical treatment: total mesorectal excision, preceded by neoadjuvant radiotherapy or chemoradiotherapy [5]. Despite a substantial rise in survival over the last two decades, the 5-year diseasespecific overall survival rate is approximately 59% for colon cancer and 61% for rectal cancer [6]. This indicates that it is very important to explore the difference between colon cancer and rectal cancer. Tumour markers are widely useful in the management of patients with tumours. Serum carcinoembryonic antigen (CEA) and cancer antigen 19–9 (CA19-9) are the most commonly used indexes in the clinical diagnosis of colorectal cancer, but both are non-specific. CEA is a glycoprotein produced by columnar and goblet cells in the normal colon cells, as well as colonic cancer cells with a half-life of 3–11 days. CA19-9 is also a glycoprotein with high molecular weight, which may be detected in the blood of gastrointestinal cancer patients [7]. We hypothesized different expressions of CEA, CA19-9, alpha-fetoprotein (AFP), cancer antigen 72–4 (CA72-4) and cancer antigen 125 II (CA125 II) between colon cancer and rectal cancer, hoping to provide reference for the different pathogenesis and treatment of these diseases. Of 219 patients with histopathologically confirmed colorectal cancer, 114 had colon cancer and 105 rectal cancer. There was no significant difference in age and gender between the colon cancer group and rectal cancer group (table 1). Five mL peripheral blood was extracted from a peripheral vein, and serum isolated by centrifugation at 2000× g for 15 min. Serum CA19-9, AFP, CA72-4 and CA125 II levels were determined by radioimmunoassay (Roche Diagnostics, Indianapolis, IN, USA), with a normal upper limit of 37 U/ml, 7 ng/ml, 6.9 U/ml and 35 U/ml, respectively. The serum CEA level was determined by ELISA (Dinabot, Tokyo, Japan), with a normal upper limit of 5 ng/ml. Statistical analysis was perfor","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1868685","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38777024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Faecal immunochemical testing (FIT): sources of result variation based on three years of routine testing of symptomatic patients in English primary care.","authors":"T James,&nbsp;B D Nicholson,&nbsp;R Marr,&nbsp;M Paddon,&nbsp;J E East,&nbsp;S Justice,&nbsp;J L Oke,&nbsp;B Shine","doi":"10.1080/09674845.2021.1896204","DOIUrl":"https://doi.org/10.1080/09674845.2021.1896204","url":null,"abstract":"<p><p><b>Introduction</b>: We aimed to determine the analytical capabilities of a commonly used faecal immunochemical test (FIT) to detect faecal haemoglobin (Hb) in symptomatic people attending primary care in the context of the English NICE DG30 guidance.<b>Materials and Methods</b>: Data obtained from independent verification studies and clinical testing of the HM-JACKarc FIT method in routine primary care practice were analysed to derive performance characteristics.<b>Results</b>: Detection capabilities for the FIT method were 0.5 µg/g (limit of blank), 1.3 µg/g (limit of detection) and 3.0 µg/g (limit of quantitation). Of 33 non-homogenized specimens, 31 (93.9%) analysed in triplicate were consistently categorized relative to 10 µg/g, compared to all 33 (100%) homogenized specimens. Imprecision was higher (median 27.8%, (range 20.5% to 48.6%)) in non-homogenized specimens than in homogenized specimens (10.2%, (7.0 to 13.5%)). Considerable variation was observed in sequential clinical specimens from individual patients but no positive or negative trend in specimen degradation was observed over time (p = 0.26).<b>Discussion</b>: The FIT immunoassay evaluated is capable of detecting faecal Hb at concentrations well below the DG30 threshold of 10 µg/g and is suitable for application in this context. The greatest practical challenge to FIT performance is reproducible sampling, the pre-analytical step associated with most variability. Further research should focus on reducing sampling variability, particularly as post-COVID-19 guidance recommends greater FIT utilization.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2021.1896204","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25401836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}