British Journal of Biomedical Science最新文献

{"title":"Changes in Mitochondrial Epigenome in Type 2 Diabetes Mellitus.","authors":"Hui Ching Low,&nbsp;William M Chilian,&nbsp;Wickneswari Ratnam,&nbsp;Tilakavati Karupaiah,&nbsp;Mohd Fairulnizal Md Noh,&nbsp;Fazliana Mansor,&nbsp;Zhi Xiang Ng,&nbsp;Yuh Fen Pung","doi":"10.3389/bjbs.2023.10884","DOIUrl":"https://doi.org/10.3389/bjbs.2023.10884","url":null,"abstract":"<p><p>Type 2 Diabetes Mellitus is a major chronic metabolic disorder in public health. Due to mitochondria's indispensable role in the body, its dysfunction has been implicated in the development and progression of multiple diseases, including Type 2 Diabetes mellitus. Thus, factors that can regulate mitochondrial function, like mtDNA methylation, are of significant interest in managing T2DM. In this paper, the overview of epigenetics and the mechanism of nuclear and mitochondrial DNA methylation were briefly discussed, followed by other mitochondrial epigenetics. Subsequently, the association between mtDNA methylation with T2DM and the challenges of mtDNA methylation studies were also reviewed. This review will aid in understanding the impact of mtDNA methylation on T2DM and future advancements in T2DM treatment.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"80 ","pages":"10884"},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9970885/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9393294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic Role of CD68⁺ and CD163⁺ Tumour-Associated Macrophages and PD-L1 Expression in Oral Squamous Cell Carcinoma: A Meta-Analysis.","authors":"Mohammed Haseeb Chohan,&nbsp;Matthew Perry,&nbsp;Paul Laurance-Young,&nbsp;Vehid M Salih,&nbsp;Andrew D Foey","doi":"10.3389/bjbs.2023.11065","DOIUrl":"https://doi.org/10.3389/bjbs.2023.11065","url":null,"abstract":"<p><p><b>Background:</b> Oral squamous cell carcinoma (OSCC) is a common malignant cancer in humans. An abundance of tumour associated macrophages (TAMs) create an immunosuppressive tumour microenvironment (TME). TAM markers (CD163 and CD68) are seen to serve as prognostic factors in OSCC. PD-L1 has seen to widely modulate the TME but its prognostic significance remains controversial. The aim of this meta-analysis is to evaluate the prognostic role of CD163⁺, CD68⁺ TAMs and PD-L1 in OSCC patients. <b>Methods:</b> Searches in PubMed, Scopus and Web of Science were performed; 12 studies were included in this meta-analysis. Quality assessment of included studies was performed according to REMARK guidelines. Risk of bias across studies was investigated according to the rate of heterogeneity. Meta-analysis was performed to investigate the association of all three biomarkers with overall survival (OS). <b>Results:</b> High expression of CD163⁺ TAMs were associated with poor overall survival (HR = 2.64; 95% Cl: [1.65, 4.23]; <i>p</i> < 0.0001). Additionally, high stromal expression of CD163⁺ TAMs correlated with poor overall survival (HR = 3.56; 95% Cl: [2.33, 5.44]; <i>p</i> < 0.00001). Conversely, high CD68 and PD-L1 expression was not associated with overall survival (HR = 1.26; 95% Cl: [0.76, 2.07]; <i>p</i> = 0.37) (HR = 0.64; 95% Cl: [0.35, 1.18]; <i>p</i> = 0.15). <b>Conclusion:</b> In conclusion, our findings indicate CD163⁺ can provide prognostic utility in OSCC. However, our data suggests CD68⁺ TAMs were not associated with any prognostic relevance in OSCC patients, whereas PD-L1 expression may prove to be a differential prognostic marker dependent on tumour location and stage of progression.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"80 ","pages":"11065"},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10310926/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10143762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Faecalibacterium prausnitzii</i> Improves Lipid Metabolism Disorder and Insulin Resistance in Type 2 Diabetic Mice.","authors":"Wenting Xuan,&nbsp;Yijing Ou,&nbsp;Wenting Chen,&nbsp;Lishan Huang,&nbsp;Chuangyu Wen,&nbsp;Guangying Huang,&nbsp;Wenting Tang,&nbsp;Daidi Zeng,&nbsp;Suran Huang,&nbsp;Lijuan Xiao,&nbsp;Zhongjun Li","doi":"10.3389/bjbs.2023.10794","DOIUrl":"https://doi.org/10.3389/bjbs.2023.10794","url":null,"abstract":"<p><p><b>Purpose:</b> Additional effective therapeutic strategies for Type 2 diabetes (T2D) patients are urgently needed<i>.</i> Gut microbiota plays an important role in T2D development and is a promising treatment strategy for T2D patients. Faecalibacterium prausnitzii (<i>F. prausnitzii</i>) is regarded as one of the most important bacterial indicators for a healthy gut, but the mechanisms of its anti-diabetic properties are still unclear. <b>Methods and Results:</b> The abundance of <i>F. prausnitzii</i> in feces of patients with T2D was detected by using qPCR. The effects of <i>F. prausnitzii</i> on glucose homeostasis, insulin resistance (IR), dyslipidemia, hepatic steatosis and inflammation were investigated in type 2 diabetic (T2D) db/db mice. We also investigated <i>F. prausnitzii</i> in people. Our results showed that the abundance of <i>F. prausnitzii</i> was significantly lower in T2D patients compared to healthy subjects. In T2D mice, we found that <i>F. prausnitzii</i> treatment significantly decreased fasting blood glucose and IR index, indicating improved glucose intolerance as well as IR. Furthermore, based on evaluation of lipid-regulating enzyme activities and proinflammatory cytokine levels, <i>F. prausnitzii</i> was not only able to improve inflammation in both adipose tissue and liver, but also ameliorate hepatic steatosis through inhibiting the activity of hepatic lipogenic enzymes. <b>Conclusion:</b> These results suggested that <i>F. prausnitzii</i> might serve as a therapeutic option for T2D by improved IR, lipid metabolism and inflammation.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"80 ","pages":"10794"},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10070466/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9271374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changing Molecular Profiles of Human Cryptosporidiosis Cases in Scotland as a Result of the Coronavirus Disease, COVID-19 Pandemic.","authors":"Ross Bacchetti,&nbsp;Lisa Connelly,&nbsp;Lynda Browning,&nbsp;Claire L Alexander","doi":"10.3389/bjbs.2023.11462","DOIUrl":"https://doi.org/10.3389/bjbs.2023.11462","url":null,"abstract":"<p><p><i>Cryptosporidium</i>, the most frequently reported parasite in Scotland, causes gastrointestinal illness resulting in diarrhoea, nausea and cramps. Two species are responsible for most cases: <i>Cryptosporidium hominis (C. hominis)</i> and <i>Cryptosporidium parvum</i> (<i>C. parvum</i>). Transmission occurs faecal-orally, through ingestion of contaminated food and water, or direct contact with faeces. In 2020, the COVID-19 pandemic led to global restrictions, including national lockdowns to limit viral transmission. Such interventions led to decreased social mixing, and reduced/no local and international travel, which are factors associated with transmission of multiple communicable diseases, including cryptosporidiosis. This report assessed the impact of the pandemic on Scottish cryptosporidiosis cases, and identified changes in circulating molecular variants of <i>Cryptosporidium</i> species. Molecular data generated using real time PCR and GP60 nested-PCR assays on laboratory-confirmed cryptosporidiosis cases reported during 2018-22 were analysed. The Scottish Microbiology Reference Laboratories (SMiRL), Glasgow, received 774 <i>Cryptosporidium</i>-positive faeces during 2018-22, of which 486 samples were successfully subtyped. During this time period, <i>C. hominis</i> (<i>n</i> = 155; 21%) and <i>C. parvum</i> (<i>n</i> = 572; 77%) were the most commonly detected species. The total number of cases during 2020, which was greatly affected by the pandemic, was markedly lower in comparison to case numbers in the 2 years before and after 2020. The most predominant <i>C. hominis</i> family detected prior to 2020 was the Ib family which shifted to the Ie family during 2022. The most common <i>C. parvum</i> variant during 2018-22 was the IIa family, however a rise in the IId family was observed (<i>n</i> = 6 in 2018 to <i>n</i> = 25 in 2022). The dominant <i>C. hominis</i> subtype IbA10G2, which accounted for 71% of <i>C. hominis</i> subtypes in 2018-19 was superseded by three rare subtypes: IeA11G3T3 (<i>n</i> = 15), IdA16 (<i>n</i> = 8) and IbA9G3 (<i>n</i> = 3) by 2022. Frequently reported <i>C. parvum</i> subtypes in 2018-19 were IIaA15G2R1 and IIaA17G1R1, accounting for 59% of total <i>C. parvum</i> subtypes. By 2022, IIaA15G2R1 remained the most common (<i>n</i> = 28), however three unusual subtypes in Scotland emerged: IIdA24G1 (<i>n</i> = 7), IIaA16G3R1 (<i>n</i> = 7) and IIaA15G1R2 (<i>n</i> = 7). Continuous monitoring of <i>Cryptosporidium</i> variants following the pandemic will be essential to explore further changes and emergence of strains with altered virulence.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"80 ","pages":"11462"},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10493326/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10244476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relevance of HLA-DP/DQ and INF-λ4 Polymorphisms to COVID-19 Outcomes.","authors":"Amany A Ghazy,&nbsp;Abdullah N Alrasheedi,&nbsp;Mohammed Elashri,&nbsp;Hany Hussein Moussa,&nbsp;Eman K Rashwan,&nbsp;Ibrahim Amer,&nbsp;Shimaa El Sharawy,&nbsp;Shimaa Elgamal,&nbsp;Salwa Tawfik,&nbsp;Mohamed Abdelnasser,&nbsp;Amel Elsheredy","doi":"10.3389/bjbs.2023.11044","DOIUrl":"https://doi.org/10.3389/bjbs.2023.11044","url":null,"abstract":"<p><p><b>Background:</b> Single nucleotide polymorphisms provide information on individuals' potential reactions to environmental factors, infections, diseases, as well as various therapies. A study on SNPs that influence SARS-CoV-2 susceptibility and severity may provide a predictive tool for COVID-19 outcomes and improve the customized coronavirus treatment. <b>Aim:</b> To evaluate the role of human leukocyte antigens DP/DQ and IFNλ4 polymorphisms on COVID-19 outcomes among Egyptian patients. <b>Participants and Methods:</b> The study involved 80 patients with severe COVID-19, 80 patients with mild COVID-19, and 80 non-infected healthy volunteers. Genotyping and allelic discrimination of HLA-DPrs3077 (G/A), HLA-DQrs7453920 (A/G), and IFNλ4 rs73555604 (C/T) SNPs were performed using real-time PCR. <b>Results:</b> Ages were 47.9 ± 8, 44.1 ± 12.1, and 45.8 ± 10 years in severe, mild and non-infected persons. There was a statistically significant association between severe COVID-19 and male gender (<i>p</i> = 0.002). A statistically significant increase in the frequency of HLA-DPrs3077G, HLA-DQrs7453920A, and IFNλ4rs73555604C alleles among severe COVID-19 patients when compared with other groups (<i>p</i> < 0.001). Coexistence of these alleles in the same individual increases the susceptibility to severe COVID-19 by many folds (<i>p</i> < 0.001). Univariate and multivariate logistic regression analysis for the studied parameters showed that old age, male gender, non-vaccination, HLA-DQ rs7453920AG+AA, HLA-DPrs3077GA+GG, and IFNλ4rs73555604CT+CC genotypes are independent risk factors for severe COVID-19 among Egyptian patients. <b>Conclusion</b>: HLA-DQ rs7453920A, HLA-DPrs3077G, and IFNλ4rs73555604C alleles could be used as markers of COVID-19 severity.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"80 ","pages":"11044"},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9894893/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9236831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CCAT 1- A Pivotal Oncogenic Long Non-Coding RNA in Colorectal Cancer.","authors":"Xiew Leng Liau,&nbsp;Shamala Salvamani,&nbsp;Baskaran Gunasekaran,&nbsp;Dinesh Kumar Chellappan,&nbsp;Anthony Rhodes,&nbsp;Vaidehi Ulaganathan,&nbsp;Yee Lian Tiong","doi":"10.3389/bjbs.2023.11103","DOIUrl":"https://doi.org/10.3389/bjbs.2023.11103","url":null,"abstract":"<p><p>Colorectal cancer (CRC) is ranked as the third most common cancer and second deadliest cancer in both men and women in the world. Currently, the cure rate and 5-year survival rate of CRC patients remain relatively low. Therefore, discovering a novel molecular biomarker that can be used to improve CRC screening, diagnosis, prognosis, and treatment would be beneficial. Long non-coding RNA colon cancer-associated transcript 1 (CCAT 1) has been found overexpressed in CRC and is associated with CRC tumorigenesis and treatment outcome. CCAT 1 has a high degree of specificity and sensitivity, it is readily detected in CRC tissues and is significantly overexpressed in both premalignant and malignant CRC tissues. Besides, CCAT 1 is associated with clinical manifestation and advanced features of CRC, such as lymph node metastasis, high tumor node metastasis stage, differentiation, invasion, and distant metastasis. In addition, they can upregulate oncogenic c-MYC and negatively modulate microRNAs <i>via</i> different mechanisms of action. Furthermore, dysregulated CCAT 1 also enhances the chemoresistance in CRC cells while downregulation of them reverses the malignant phenotypes of cancer cells. In brief, CCAT 1 serves as a potential screening, diagnostic and prognostic biomarker in CRC, it also serves as a potential therapeutic marker to treat CRC patients.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"80 ","pages":"11103"},"PeriodicalIF":1.9,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10070472/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9276695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intermittent Short-Duration Re-oxygenation Attenuates Cardiac Changes in Response to Hypoxia: Histological, Ultrastructural and Oxidant/Antioxidant Parameters.","authors":"Ayed A Shati,&nbsp;Mohamed Samir A Zaki,&nbsp;Youssef A Alqahtani,&nbsp;Mohamed A Haidara,&nbsp;Mohammed A Alshehri,&nbsp;Amal F Dawood,&nbsp;Refaat A Eid","doi":"10.3389/bjbs.2022.10150","DOIUrl":"https://doi.org/10.3389/bjbs.2022.10150","url":null,"abstract":"<p><p><b>Context:</b> Intermittent short-duration re-oxygenation attenuates cardiac changes in response to hypoxia. <b>Objective:</b> To see if intermittent short-duration re-oxygenation may protect the heart muscle from hypoxia damage. <b>Materials and Methods:</b> Eighteen albino rats were used to carry out the study. Rats divided into: (normoxia); rats exposed to room air as a control, second (hypoxic) group; rats subjected to a pressure of 405 mmHg in a hypobaric chamber to simulate hypoxia at 5,000 m, and third (intermittent short-duration re-oxygenation); rats exposed to room air three times per day. Experiments were all 14 days long. <b>Results:</b> Hypoxia enhanced the oxidative stress biomarker malondialdehyde while lowering the antioxidant superoxide dismutase . The levels of tumour necrosis factor (TNF-α) and interleukin-6 (IL-6) in the myocardium were elevated in hypoxic hearts. The hypoxic rats' cardiac myofibrils showed disarray of muscle fibres, vacuolation of the sarcoplasm, pyknosis of the nucleus, and expansion of intercellular gaps on histological examination. In addition, cardiomyocytes showed degenerative defects in ventricular myocardial cells on ultrastructural analysis. Myofibril thinning and degenerative mitochondrial changes affected intercalated discs with fascia adherent, desmosomes, and gap junction. Intermittent short-duration re-oxygenation improve cardiac histological, ultrastructural and oxidant/antioxidant parameters changes during hypoxia. <b>Conclusion:</b> Hypoxia showed a substantial impact on myocardial architecture, as well as increased oxidative stress and pro-inflammatory cytokines. Intermittent short-duration re-oxygenation significantly decreases hypoxia-induced cardiac changes.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"79 ","pages":"10150"},"PeriodicalIF":1.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9302540/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9828773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leukocytosis induced by tigecycline in two patients with severe acute pancreatitis.","authors":"X Li,&nbsp;L Li,&nbsp;T Liu,&nbsp;X Hai,&nbsp;B Sun","doi":"10.1080/09674845.2021.1885865","DOIUrl":"https://doi.org/10.1080/09674845.2021.1885865","url":null,"abstract":"Department of Pharmacy, The First Affiliated Hospital of Harbin Medical University, Harbin, China; Department of Pancreatic and Biliary Surgery, The First Affiliated Hospital of Harbin Medical University; Key Laboratory of Hepatosplenic Surgery, Ministry of Education, The First Affiliated Hospital of Harbin Medical University, Harbin, China; Department of Pharmacy, Harbin Medical University Cancer Hospital, Harbin, China","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"78 4","pages":"225-228"},"PeriodicalIF":1.9,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2021.1885865","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25378830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increased serum CA125 II, but not CEA，CA19-9，AFP or CA72-4 in colon cancer compared to rectal cancer.","authors":"T Liu,&nbsp;X Li,&nbsp;D Liu,&nbsp;S Liu,&nbsp;M Dong","doi":"10.1080/09674845.2020.1868685","DOIUrl":"https://doi.org/10.1080/09674845.2020.1868685","url":null,"abstract":"Globally, colorectal cancer is the third most frequent cancer type, with &gt;1.4 million new cases and &gt;690,000 deaths annually [1]. Survival from colorectal cancer is significantly dependent on the stage at diagnosis, with the 5-year rate at ~90% for localized disease, 70% for regional disease and 13% for distantly metastatic disease [2]. Several screening tests, including faecal occult blood test and colonoscopy, are frequently used in the detection of colorectal cancer. However, none are established and well-accepted screening tools due to their invasiveness, high cost or low sensitivity [3]. Therefore, the search for more sensitive, easily detected and representative biomarkers is of great significance for the early diagnosis and monitoring of this disease. Several biological and clinical hallmarks indicate that rectal cancer is different from colon cancer. The rectum and colon have a different embryological origin, anatomy and function [4]. Consequently, the treatments for primary rectal and colon cancer are different. Primary rectal cancer requires specific surgical treatment: total mesorectal excision, preceded by neoadjuvant radiotherapy or chemoradiotherapy [5]. Despite a substantial rise in survival over the last two decades, the 5-year diseasespecific overall survival rate is approximately 59% for colon cancer and 61% for rectal cancer [6]. This indicates that it is very important to explore the difference between colon cancer and rectal cancer. Tumour markers are widely useful in the management of patients with tumours. Serum carcinoembryonic antigen (CEA) and cancer antigen 19–9 (CA19-9) are the most commonly used indexes in the clinical diagnosis of colorectal cancer, but both are non-specific. CEA is a glycoprotein produced by columnar and goblet cells in the normal colon cells, as well as colonic cancer cells with a half-life of 3–11 days. CA19-9 is also a glycoprotein with high molecular weight, which may be detected in the blood of gastrointestinal cancer patients [7]. We hypothesized different expressions of CEA, CA19-9, alpha-fetoprotein (AFP), cancer antigen 72–4 (CA72-4) and cancer antigen 125 II (CA125 II) between colon cancer and rectal cancer, hoping to provide reference for the different pathogenesis and treatment of these diseases. Of 219 patients with histopathologically confirmed colorectal cancer, 114 had colon cancer and 105 rectal cancer. There was no significant difference in age and gender between the colon cancer group and rectal cancer group (table 1). Five mL peripheral blood was extracted from a peripheral vein, and serum isolated by centrifugation at 2000× g for 15 min. Serum CA19-9, AFP, CA72-4 and CA125 II levels were determined by radioimmunoassay (Roche Diagnostics, Indianapolis, IN, USA), with a normal upper limit of 37 U/ml, 7 ng/ml, 6.9 U/ml and 35 U/ml, respectively. The serum CEA level was determined by ELISA (Dinabot, Tokyo, Japan), with a normal upper limit of 5 ng/ml. Statistical analysis was perfor","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"78 4","pages":"218-220"},"PeriodicalIF":1.9,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1868685","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38777024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association analysis of KISS1 polymorphisms and haplotypes with polycystic ovary syndrome.","authors":"M Farsimadan,&nbsp;F Moammadzadeh Ghosi,&nbsp;S Takamoli,&nbsp;H Vaziri","doi":"10.1080/09674845.2020.1864109","DOIUrl":"https://doi.org/10.1080/09674845.2020.1864109","url":null,"abstract":"<p><strong>Introduction: </strong><i>KISS1</i> play an essential role in human reproductive functions by regulating the hypothalamic-pituitary-gonadal axis. Loss-of-function mutations in this gene have been frequently identified in patients with different reproductive disorders. We hypothesised links between <i>KISS1</i> polymorphisms and polycystic ovary syndrome (PCOS).</p><p><strong>Materials and methods: </strong>In order to find links between <i>KISS1</i> polymorphisms rs4889 C > G, rs12998 G > A, and rs35431622 A > G with PCOS, 770 blood samples were obtained from 385 control and 385 PCOS women. DNA was extracted, and genotyped for <i>KISS1</i> variants by PCR.</p><p><strong>Results: </strong>rs12998 G > A was linked to PCOS in dominant (p < 0.001), recessive (p < 0.001), co-dominant (p < 0.001), and allelic models (p < 0.001). In addition, rs4889 C > G was linked in recessive, dominant, co-dominant, and allelic models (p < 0.001). rs35431622 A > G was not linked to PCOS. Further analysis indicated that C-G-G haplotype was more common and G-A-G haplotype was less prevalent in cases compared with controls.</p><p><strong>Conclusion: </strong><i>KISS1</i> variants rs12998 G > A and rs4889 C > G may be linked to the pathophysiology of PCOS.</p>","PeriodicalId":9236,"journal":{"name":"British Journal of Biomedical Science","volume":"78 4","pages":"201-205"},"PeriodicalIF":1.9,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/09674845.2020.1864109","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38700882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}