Biomedical research and clinical practice最新文献_第3页

Screening of Angelman Syndrome deletion and methylation aberration using MS-MLPA assay in a Tunisian population 在突尼斯人群中使用MS-MLPA法筛选Angelman综合征缺失和甲基化畸变

Biomedical research and clinical practice Pub Date : 2019-01-01 DOI: 10.15761/brcp.1000188

W. Manoubi, A. Rouissi, D. Hmida, A. Mili, Ilhem B Charfeddine, A. Kdissa, Rihab B Sgaier, Hatem Elghezel, I. Turki, N. Gueddiche, Nejla Soyah, S. Mougou, A. Saâd, M. Gribaa

{"title":"Screening of Angelman Syndrome deletion and methylation aberration using MS-MLPA assay in a Tunisian population","authors":"W. Manoubi, A. Rouissi, D. Hmida, A. Mili, Ilhem B Charfeddine, A. Kdissa, Rihab B Sgaier, Hatem Elghezel, I. Turki, N. Gueddiche, Nejla Soyah, S. Mougou, A. Saâd, M. Gribaa","doi":"10.15761/brcp.1000188","DOIUrl":"https://doi.org/10.15761/brcp.1000188","url":null,"abstract":"Background: Angelman syndrome (AS) is a neurodevelopmental disorder characterized by severe mental retardation, absent speech, dysmorphic facial features, microcephaly, epileptic seizures, Electroencephalography (EEG) abnormalities and neurological problem. Four known molecular mechanisms lead to a deficiency in maternal UBE3A expression and consequently to AS: (1) Deletion of the AS critical region on the maternal chromosome 15q11.2–q13 (70%), (2) paternal uniparental disomy (pUPD) (2-7%), (3) imprinting defects (3–5%), and (4) mutations in the maternal copy of UBE3A (10%). Materials and methods: Here, we report 11 Tunisian AS patients suspected on the basis of clinical features, behavior, EEG findings and confirmed by molecular analysis using FISH technique, microsatellites study and Methylation Specific Multiplex Ligation-dependent Probe Amplification (MS-MLPA). Results: The diagnosis was confirmed in these patients (7 males, 4 females) by detecting the presence of deletion of the critical AS region on chromosome 15 through the use of fluorescence in situ hybridization (FISH) technique in 10 patients, and confirmed by Methylation-Specific Multiplex Ligation-dependent Probe Amplification (MS-MLPA). A microsatellite analysis detected only one patient with uniparental disomy. Conclusion: Deletion and methylation aberration screening by MS-MLPA assay is considered as a rapid and cost-effective method to confirm Angelman syndrome diagnosis contributing to an early interventional therapy and genetic counseling should be provided. *Correspondence to: Wiem Manoubi, Laboratory of Human Cytogenetics, Molecular Genetics and Reproductive Biology, Farhat Hached University Hospital, Sousse, Tunisia, Tel: +216 73 102 500; E-mail: wiem.manoubi@yahoo.fr","PeriodicalId":92336,"journal":{"name":"Biomedical research and clinical practice","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67430810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gram-positive uropathogens: Empirical treatment and emerging antimicrobial resistance 革兰氏阳性尿路病原体:经验性治疗和新出现的抗菌素耐药性

Biomedical research and clinical practice Pub Date : 2019-01-01 DOI: 10.15761/brcp.1000182

U. Urmi, N. Jahan, S. Nahar, M. Rana, F. Sultana, B. Hossain, S. Iqbal, M. Hossain, A. Mosaddek, Salequl Islam

{"title":"Gram-positive uropathogens: Empirical treatment and emerging antimicrobial resistance","authors":"U. Urmi, N. Jahan, S. Nahar, M. Rana, F. Sultana, B. Hossain, S. Iqbal, M. Hossain, A. Mosaddek, Salequl Islam","doi":"10.15761/brcp.1000182","DOIUrl":"https://doi.org/10.15761/brcp.1000182","url":null,"abstract":"Urinary tract infection (UTI) is a global problem. Most UTI research focuses on gram-negative etiology. Enterobacteriaceae was found to be the most prevalent UTI infection constituting more than 80% of all the reported cases. The major gram-positive bacteria in UTI cases are Staphylococcus saprophyticus, Enterococcus faecalis, Streptococcus agalactiae. Gram-positive pathogens were reported in multiple countries in both uncomplicated and complicated UTI. Antibiotic therapy of gram-positive bacteria is completely different than that of gram-negative UTI pathogens. However, symptoms associated with UTI caused by gram-positive and gram-negative are very similar. Without proper diagnosis, there is a high possibility of getting the wrong diagnosis and subsequent antibiotic therapy. Very limited studies are available focusing etiology and their antibiotic susceptibilities in Bangladesh perspective. We aimed to seek in this gap-filling research area. This study has detected 8.2% of gram-positive bacteria in UTI patients. The prevalence shows the harmony with the earlier published reports. Male and female were found to be infected equally by gram-positive UTI pathogens. Most of the earlier publication shows that female is more vulnerable to gram-negative UTI bacteria. Unlikely, our findings look males are equally vulnerable by gram-positive in comparison to females. Further studies with more sample sizes can warrant the preliminary findings. Antibiogram analyses showed amikacin and gentamicin as the most effective antibiotics against our tested isolates. In contrast, nitrofurantoin was found the most ineffective drug in this study. Findings of the study could help in prescribing antibiotics from this evidence-based study. *Correspondence to: Umme Laila Urmi, Department of Microbiology, Jahangirnagar University, Savar, Dhaka, Bangladesh, Tel: +880-1725996390; E-mail: ummelailaurmi@gmail.com","PeriodicalId":92336,"journal":{"name":"Biomedical research and clinical practice","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67430834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Brain segmentation tools under uncertain conditions for radiotherapy treatment planning 不确定条件下的脑分割工具用于放射治疗计划

Biomedical research and clinical practice Pub Date : 2019-01-01 DOI: 10.15761/brcp.1000183

S. Zimeras

{"title":"Brain segmentation tools under uncertain conditions for radiotherapy treatment planning","authors":"S. Zimeras","doi":"10.15761/brcp.1000183","DOIUrl":"https://doi.org/10.15761/brcp.1000183","url":null,"abstract":"Objective : Radiotherapy Treatment Planning (RTP) is a procedure to plan the irradiation treatment which is usually simulated on a conventional simulator before applying it on the patient. The main goal is to deliver the adequate irradiation dose to a tumour without causing severe damage to surrounding normal and health tissues. Material and methods : The major weaknesses of current RTP systems come from their rendering methods since most of them use surface rendering rather than volume rendering. All target objects and other critical organs are required to be modelled with interactive contouring slice by slice. The sizes of the segmentation objects are not accurate and some of small but critical organs sometimes maybe neglected. Results : Image segmentation is currently used into several medical imaging applications that involve diagnosis or treatment. Segmentation of volumes is an essential tool for the radiation therapy treatment of the cancer. One of the key organs that must be protected during the irradiation treatment is the brain. Nowadays, high resolution computed tomography (CT) data are required to perform accurate 3D treatment planning, and therefore there is the demand for quick but at the same time accurate segmentation tools. Inappropriate contours results have been performed for the cases where uncertain conditions are appeared (like position of the body in the table and metal material of the bed). Conclusions : In this work we presented an algorithm that can be used for the accurate semi-automatic segmentation of the brain in three dimensions (3D) from CT images. Our method, which is currently in clinical use, is basically composed from an edge detection algorithm and statistical extreme values techniques (outliers).","PeriodicalId":92336,"journal":{"name":"Biomedical research and clinical practice","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67430436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Evaluation of repellent activities of the essential oil of Ocimum basilicum against Anopheles mosquito and formulation of mosquito repellent cream 罗勒精油对按蚊驱避活性的评价及驱蚊膏的研制

Biomedical research and clinical practice Pub Date : 2019-01-01 DOI: 10.15761/brcp.1000184

Almardi A Adam, S. A. Ahmed, T. A. Mohamed,, Rasha A Azrag, Salma E Mustfa, Omer Aa Hamdi

{"title":"Evaluation of repellent activities of the essential oil of Ocimum basilicum against Anopheles mosquito and formulation of mosquito repellent cream","authors":"Almardi A Adam, S. A. Ahmed, T. A. Mohamed,, Rasha A Azrag, Salma E Mustfa, Omer Aa Hamdi","doi":"10.15761/brcp.1000184","DOIUrl":"https://doi.org/10.15761/brcp.1000184","url":null,"abstract":"This study was conducted to evaluate the repellent activities of the essential oils of Ocimum basilicum against Anopheles mosquito and to formulate cream repellent for mosquitoes from essential oil of O. basilicum L . Ocimum basilicum L. (Lamiaceae) commonly known as sweet basil, has been used as a traditional medicinal plant for the treatment of headaches, coughs, diarrhoea, constipation, warts, worms, and kidney malfunctions.The essential oil of the plant of O. basilicum was obtained by hydro-distillation and analyzed by gas chromatography coupled to mass spectrometry (GC-MS).The physical properties of essential oil were also identified. Fifty-one compounds were characterized and identified by GC–MS, comprising 99.7% of the total oil. Major compounds were linalool (18.88%), cinnamic acid (18.31%), and eucalyptol (8.46%). In this work, the oil was assessed for topical repellence effects against malarial vector Anopheles mosquito in cages. The essential oil was tested at three different concentrations 2, 4 and 6% of the oil. From the results obtained, the essential oil of Ocimum basilicum exhibited relatively high repellency effect (> 250 minutes at 6% concentration). The oil was successively formulated as a cream. The formulated cream was prepared using the concentration of 6% of the essential oil. The cream was tested for repellency effect and showed same effect as the essential oil of Ocimum basilicum at the concentration of 6%. The results achieved suggest that essential oils of Ocimum basilicum have activities repellency against female Anopheles and could be used in the form of natural repellent cream.","PeriodicalId":92336,"journal":{"name":"Biomedical research and clinical practice","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67430524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Biochemical effects of nandrolone decanoate abuse on experimental animal model 滥用癸酸诺龙对实验动物模型的生化影响

Biomedical research and clinical practice Pub Date : 2019-01-01 DOI: 10.15761/brcp.1000185

Neveen A. Salem, Hanan S. Alnahdi

引用次数: 1

A comparative study of the antimicrobial activity of oregano, rosemary and thyme essential oils against Salmonella spp 牛至、迷迭香和百里香精油对沙门氏菌抑菌活性的比较研究

Biomedical research and clinical practice Pub Date : 2019-01-01 DOI: 10.15761/brcp.1000197

Grammato Evangelopoulo, N. Solomakos, Anastasios Ioannidis, A. Pexara, A. Burriel

{"title":"A comparative study of the antimicrobial activity of oregano, rosemary and thyme essential oils against Salmonella spp","authors":"Grammato Evangelopoulo, N. Solomakos, Anastasios Ioannidis, A. Pexara, A. Burriel","doi":"10.15761/brcp.1000197","DOIUrl":"https://doi.org/10.15761/brcp.1000197","url":null,"abstract":"The emergence of Salmonella resistance to therapeutic agents against human infections is attributed to their excessive use in the treatment of food-producing animals. The spreading of resistance necessitates vigorous research on alternative therapeutic schemes in order to replace commonly used antimicrobials. Thus, the aim of the present study was to investigate the inhibitory activity of three commercially available essential oils (EOs) against fifty-nine Salmonella strains belonging to fourteen serovars isolated from pig carcasses at slaughter. Their antimicrobial activity was compared with that of twenty-four commonly used antimicrobials previously tested against the same strains. The three chosen EOs were oregano, rosemary and thyme. Oregano and thyme EOs showed high antimicrobial activity, compared to that of rosemary and a better antimicrobial effect compared to that of commercial antimicrobials. The results showed that oregano and thyme have the potential of an effective alternative treatment against Salmonella spp., especially those strains resistant to synthetic antimicrobials. Thus, some EOs could be a safer alternative treatment against certain infections of food-producing animals or, perhaps, growth promoters, used for effective prevention. *Correspondence to: Grammato Evangelopoulou, Department of Nursing, Faculty of Human Movement and Quality of Life Sciences, Laboratory of Integrated Healthcare, University of Peloponnese, Sparta, Greece, Tel: 30-694584-7840, E-mail: matinavet@hotmail.com","PeriodicalId":92336,"journal":{"name":"Biomedical research and clinical practice","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67431232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Shingles in a pediatric healthcare provider: Can patient care continue? 带状疱疹在儿科医疗保健提供者:病人护理可以继续吗?

Biomedical research and clinical practice Pub Date : 2019-01-01 DOI: 10.15761/brcp.1000177

L. Mazur, E. Reznicek, S. Wootton

引用次数: 0

Sex-specific activity of hesperidin, diosmin and genistein on human umbilical vein 橙皮苷、薯蓣皂苷和染料木素对人脐静脉的性别特异性活性

Biomedical research and clinical practice Pub Date : 2019-01-01 DOI: 10.15761/brcp.1000196

C. Pinna, A. Sala

{"title":"Sex-specific activity of hesperidin, diosmin and genistein on human umbilical vein","authors":"C. Pinna, A. Sala","doi":"10.15761/brcp.1000196","DOIUrl":"https://doi.org/10.15761/brcp.1000196","url":null,"abstract":"Flavonoids intake may be associated to beneficial effects on the cardiovascular system. Aim of this study was to investigate biological activities and mechanism of action of two Citrus-derived flavonoids, hesperidin and diosmin, and the estrogenic isoflavone genistein on vascular tone of human umbilical vein (HUV). Umbilical cords obtained at delivery from healthy women were divided into two groups according to the sex of the new-born. Experiments with flavonoids were carried out in serotonin (5-HT)-precontracted HUV rings, before and after tissue pre-treatment with L-NAME (NOS inhibitor) or glybenclamide (K ATP channel blocker). Hesperidin and genistein evoked larger NO-dependent vasorelaxation than diosmin. Endothelium-dependent responses to hesperidin and genistein, but not to diosmin, were gender-specific, being larger in female- as compared with male-derived HUV. Pre-exposure of tissues to L-NAME significantly impaired relaxant responses to hesperidin and genistein and completely blocked those to diosmin. Residual vasorelaxations elicited by hesperidin and genistein after L-NAME pre- treatment were blocked by incubation with glybenclamide. In conclusion, while all flavonoids studied evoked NO-dependent vasorelaxation in HUV rings, the effects of hesperidin and genistein were gender-specific and greater than that of diosmin. Hesperidin, diosmin, and genistein might exert a beneficial effect on the vascular and endothelial function of HUV. This information may be important for foetal medicine and practice regarding the effects on foetal development of umbilical vascular functions.","PeriodicalId":92336,"journal":{"name":"Biomedical research and clinical practice","volume":"57 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67431052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Lactational nicotine alters pancreatic histomorphology and carbohydrate metabolism in Wistar rats 哺乳期尼古丁改变Wistar大鼠胰腺组织形态学和碳水化合物代谢

Biomedical research and clinical practice Pub Date : 2019-01-01 DOI: 10.15761/brcp.1000187

O. Gabriel, Shinshima, Iveren, S. Fatima, Gbadamosi Ismail, Amedu Nathaniel, K. Risikat

引用次数: 0

The chromosomal effects of GSM-like electromagnetic radiation exposure on human fetal cells gsm样电磁辐射暴露对人胎儿细胞的染色体效应

Biomedical research and clinical practice Pub Date : 2019-01-01 DOI: 10.15761/brcp.1000192

Nuri Uslu, O. Demirhan, M. Emre, G. Seydaoglu

引用次数: 4