Biomedical research and clinical practice最新文献

{"title":"Bacterial, viral, and fungal infections among patients with coronavirus disease 2019: A mini-review","authors":"Sabah Mohamed Alharazy","doi":"10.15761/brcp.1000221","DOIUrl":"https://doi.org/10.15761/brcp.1000221","url":null,"abstract":"","PeriodicalId":92336,"journal":{"name":"Biomedical research and clinical practice","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67432163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resolution of clinical signs, a complete response, and long-term survival (> 23 Years) in a 3 and ½ month female with a newly diagnosed diffuse intrinsic pontine glioma treated with antineoplastons","authors":"Stanislaw R. Burzynski, T. Janicki, Gregory S. Burzynski, S. Beenken","doi":"10.15761/brcp.1000220","DOIUrl":"https://doi.org/10.15761/brcp.1000220","url":null,"abstract":"","PeriodicalId":92336,"journal":{"name":"Biomedical research and clinical practice","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67432084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hypoglycaemia in the early neonatal period in newborns with risk factors","authors":"Tatyana E. Taranushenko, Ekaterina V. Antsiferova, N. G. Kiseleva","doi":"10.15761/brcp.1000217","DOIUrl":"https://doi.org/10.15761/brcp.1000217","url":null,"abstract":"","PeriodicalId":92336,"journal":{"name":"Biomedical research and clinical practice","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67432351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A controlled respiratory training to improve quality of life and well-being perception in chronic obstructive pulmonary disease patients","authors":"F. Mastorci, F. Mannucci, Maria Francesca Lodovica Lazzeri, M. Passera, Fabio Brucini, I. Pavlickova, A. Pingitore, G. Catapano","doi":"10.15761/brcp.1000218","DOIUrl":"https://doi.org/10.15761/brcp.1000218","url":null,"abstract":"","PeriodicalId":92336,"journal":{"name":"Biomedical research and clinical practice","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67432205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polymer microbubbles loaded with gold nanoparticles as hybrid contrast agent for computed tomography and ultrasound","authors":"Hongjian Chen, Ying-zheng Zhao, D. Grishenkov","doi":"10.15761/brcp.1000203","DOIUrl":"https://doi.org/10.15761/brcp.1000203","url":null,"abstract":"Microbubbles (MBs) with size below 10 µm are commonly used as an ultrasound contrast agent (UCA). The aim of the novel UCA developed in our lab is to support imaging modalities other than ultrasound to form hybrid contrast agents. The hybrid contrast agents through the synergistic effect can potentially improve the diagnostic outcome of the combined multimodal imaging technique. In this study, we modified the polyvinyl alcohol (PVA) MB fabrication protocol to encapsulate the gold nanoparticles into the shell and also in the core of the MBs. Furthermore, we evaluated the morphology, nonlinear ultrasound response, and X-ray property of dual modal contrast agents. The results revealed that the loading of the gold nanoparticles into the PVA MB core is a promising step towards the development of the dual modal contrast agent.","PeriodicalId":92336,"journal":{"name":"Biomedical research and clinical practice","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67431826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro evaluation of Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans polymicrobial biofilm growth on synthetic surgical implant materials","authors":"B. Colgan, Brooke A Pati, W. Kurata, Timothy S. Horseman, L. Pierce","doi":"10.15761/brcp.1000206","DOIUrl":"https://doi.org/10.15761/brcp.1000206","url":null,"abstract":"Biofilms are a known important contributor to the infectious complications associated with prosthetic mesh implantation. Previous studies have demonstrated the formation of monomicrobial biofilms on surgical mesh materials by different bacterial species in vitro , but a paucity of data exists examining polymicrobial biofilm formation by antibiotic-resistant organisms, which may be more clinically relevant. Therefore, the aim of this study was to evaluate the ability of three clinical bacterial and fungal isolates to form mixed-species and single-species biofilms on five synthetic implant materials in vitro , including monofilament polypropylene, monofilament polyester, multifilament polyester, monofilament polytetrafluoroethylene (PTFE), and silicone. Methicillin-resistant Staphylococcus aureus (MRSA), multidrug-resistant Pseudomonas aeruginosa , and Candida albicans (alone or in combination) were inoculated into culture medium containing meshes and allowed to attach and propagate into mature biofilms for 48 hours at 37 o C. Additional samples were inoculated with Staphylococcus epidermidis for comparison. Biofilm biomass and the number of viable cells were quantified by crystal violet staining and colony forming units (CFU) counting, respectively. Images of polymicrobial biofilms were obtained using fluorescence microscopy of FM ® 1-43-stained meshes. All materials investigated were capable of harboring biofilms, but the relative abundance was dependent upon both material and organism type. Overall, PTFE meshes exhibited the highest levels of polymicrobial biofilm formation. When comparing monomicrobial biofilms, Pseudomonas aeruginosa demonstrated greater biomass than MRSA and Staphylococcus epidermidis on PTFE, polypropylene, and monofilament polyester, while MRSA and Staphylococcus epidermidis biomass did not differ significantly among mesh types. Thus MRSA, Pseudomonas aeruginosa , and Candida albicans can cooperatively form mature biofilms on surgical implant materials, but different mesh constructs vary in their susceptibility to biofilm formation depending upon the organism(s) present. This suggests that the type of implant material chosen for surgical procedures may affect the patient’s wound healing response and clinical outcome, particularly when used in a contaminated environment.","PeriodicalId":92336,"journal":{"name":"Biomedical research and clinical practice","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67431432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regenerative Surgery Unit (RSU) a new model of care","authors":"V. Nicola","doi":"10.15761/BRCP.1000209","DOIUrl":"https://doi.org/10.15761/BRCP.1000209","url":null,"abstract":"Regenerative Surgery (RS) defines all the Regenerative Medicine (RM) treatments that can be applied through minimally invasive surgical procedures. This biomedical approach includes the use of stem cells, various local biochemical mediators, and tissue engineering. The Regenerative Surgery Unit (RSU) is a new model of health care governance integrating clinical, scientific and research activities. RSU works in a multidisciplinary setting involving surgeon , biologist, immunologist, laboratory technician, radiologist, and physiotherapist. RSU setting also aims to generate a special relation between doctor and patient. The aim of this relationship is to establish a solid and mutually beneficial alliance, founded on medical ethics, between doctor and patient. RSU organises clinical services through ambulatory care and day surgery settings. The scientific and research activities are integrated as components of daily medical practice. This article describes some established procedures in RS and some refinements that are more recently available as a result of our clinical and research experience in RM fields. Health Tourism is one element of the sustainable growth model we are developing in the RSU - Villa Aurora Hospital, Foligno (Italy).","PeriodicalId":92336,"journal":{"name":"Biomedical research and clinical practice","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67431704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Meta-analysis of immune signaling pathways between human type 2 diabetic tissue and mouse bone repair","authors":"James McCauley, A. Walsh, J. F. Bejar, Jason Ianni, M. Georges, Zaneta Zachwieja, R. Gray, Tinchun Chu, J. Cottrell, Sulie L. Chang","doi":"10.15761/brcp.1000202","DOIUrl":"https://doi.org/10.15761/brcp.1000202","url":null,"abstract":"Type II diabetes mellitus (T2DM) is a chronic metabolic disorder characterized by insulin insensitivity, hyperglycemia, and immune dysregulation. Recent findings have shown that T2DM has a significant impact on the skeletal system, including the impairment of the fracture healing process which commonly leads to nonunion. Throughout the process, heterotypic interactions between different immune cells are required for the recruitment and differentiation of osteogenic cells vital for fracture repair. The purpose of this study was to compare inflammatory gene expression induced in T2DM with those occurring during fracture repair with a specific focus on immune cell expression. Using publicly available RNA-seq datasets and Ingenuity Pathway Analysis (IPA), we compared gene expression profiles of human diabetic and non-diabetic data to gene expression profiles of mice post-fracture. IPA core analysis of diabetic vs. non-diabetic immune gene expression revealed top canonical pathways (p-value < 1.0 x 10-6) involved in the Th1 Activation Pathway, Granulocyte Adhesion and diapedesis, and IL-7 Signaling, had an average activation z-score of -2.373, thus exhibiting a predicted inhibition when compared to non-diabetic controls. Additionally, top upstream inflammatory regulators such as TNF-α, IL-1B, and IL-6 also exhibited an average 3.5 log-fold reduction in expression. When examining gene expression in normal fracture repair, previous upstream inflammatory regulators exhibit an average 2.1 log-fold increase. Our results suggest that during fracture repair, the early immune response required for recruitment of osteogenic cells and repair is impaired in T2DM signaling. *Correspondence to: Sulie L. Chang, Seton Hall University, 400 South Orange Avenue, South Orange, NJ, USA, Tel: 973-761-9456; E-mail: sulie.chang@shu.edu","PeriodicalId":92336,"journal":{"name":"Biomedical research and clinical practice","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67431783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Burmese grape, common lime, pomelo, salak, Southern langsat, star fruit, and orange modulate the expression of cytochrome P450 enzymes in mouse livers","authors":"K. Jarukamjorn, W. Chatuphonprasert","doi":"10.15761/BRCP.1000210","DOIUrl":"https://doi.org/10.15761/BRCP.1000210","url":null,"abstract":"Cytochrome P450 isoenzymes (CYPs) is responsible for xenobiotic and drug metabolism, and highly expressed in liver. Diet and daily dietary supplements might modify the expression of CYPs, leading to the undesirable side-effects of drugs. The effects of eight Thai sour fruits, namely Burmese grape (Baccaurea ramiflora Lour.), common lime (Citrus aurantifolia Linn.), pomelo (Citrus maxima (Burm) Merrill), salak (Salacca zalacca (Gaertn.) Voss), Southern langsat (Lansium domesticum Corr.), special honey orange (Lonicera japonica Thunb.), star fruit (Averrhoa carambola Linn.), and Thanathon orange (Citrus sinensis Osb.), on the expression of hepatic CYPs were determined. Effects of the aqueous extract of all fruits (0.8 to 25 mg/mL) on the CYPs enzyme activities were determined in vitro using the reactions established enzymatic assays (EROD, MROD, BROD/PROD, and ENDM). The fruit, which possessed the most potent inhibitory activity, was selected to determine its effect in vivo on the expression and activities of CYPs in mouse livers. All eight Thai sour fruits were found to inhibit the activities of Cyp1a1, Cyp1a2, Cyp2b9/10, and Cyp3a via inhibition of EROD (IC50 = 0.001 – 25.91 mg/mL), MROD (IC50 = 0.681 – 84.95mg/mL), BROD (IC50 = 0.221 – 52.28 mg/ mL), PROD (IC50 = 0.111 – 64.51 mg/mL), and ENDM (IC50 = 34.27 – 59.62 mg/mL) reactions, respectively. Burmese grape exhibited the most potent inhibitory activity and inhibited the largest number of CYPs. Burmese grape was therefore selected for further in vivo study, the fruit juice being tested at a dose of 250, 500, and 1,000 mg/kg/day for 7 and 28 days. The highest dose of Burmese grape (1,000 mg/kg/day) induced Cyp1a1, Cyp1a2, and Cyp2b9/10 activities. However, Cyp3a11 activity was significantly inhibited by Burmese grape treatments. Hence, Thai sour fruit consumption, especially Burmese grape, may induce food-drug interactions, particularly if they are consumed for a long time and in large quantities. *Correspondence to: Waranya Chatuphonprasert, Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand, Tel: +66-43-722-393; E-mail: waranya.c@msu.ac.th","PeriodicalId":92336,"journal":{"name":"Biomedical research and clinical practice","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67431714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flatus and toilet air may be routes for faecal-oral infection","authors":"Ryoichi Matsuda","doi":"10.15761/BRCP.1000216","DOIUrl":"https://doi.org/10.15761/BRCP.1000216","url":null,"abstract":"Flatus that passes through an individual’s clothing into the atmosphere contains a substantial number of enteric bacteria. Airborne bacteria were also found in toilet air after defecation. From the literature, SARS-CoV-2 RNA has been found in patients’ stool and sewage. Although the present study did not attempt to isolate any SARS-CoV-2, the results suggest that flatus and toilet air are potential routes for fecal and oral transmission of SARS-CoV-2. The new variants are shown to spread more easily between humans, and this hidden route should be aware of virus transmission. *Correspondence to: Ryoichi Matsuda, Department of Science Education, Graduate School of Science, Tokyo University of Science, 1-3 Kagurazaka, Shinjuku-ku, Tokyo 162-8601, Japan, Tel: +81-3-5228-8279, Fax: +81-3-5228-8279; E-mail: cmatsuda@ rs.tus.ac.jp","PeriodicalId":92336,"journal":{"name":"Biomedical research and clinical practice","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67432135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}