Brazilian dental journal最新文献_第4页

EGFR, VEGF, and angiogenesis promote the development of lipoma in the oral cavity. EGFR, VEGF和血管新生促进口腔脂肪瘤的发展。

Brazilian dental journal Pub Date : 2023-03-01 DOI: 10.1590/0103-6440202305117

Weslay Rodrigues da Silva, Hévila de Figueiredo Pires, Glória Maria de França, Roseana de Almeida Freitas, Hannah Gil de Farias Morais, Hébel Cavalcanti Galvão

{"title":"EGFR, VEGF, and angiogenesis promote the development of lipoma in the oral cavity.","authors":"Weslay Rodrigues da Silva, Hévila de Figueiredo Pires, Glória Maria de França, Roseana de Almeida Freitas, Hannah Gil de Farias Morais, Hébel Cavalcanti Galvão","doi":"10.1590/0103-6440202305117","DOIUrl":"https://doi.org/10.1590/0103-6440202305117","url":null,"abstract":"This study aimed to detect, quantify and compare the immunohistochemical expression of EGFR and VEGF and microvessel count (MVC) in oral lipomas, and to correlate the findings with clinical and morphological characteristics of the cases studied. The sample consisted of 54 oral lipomas (33 classic and 21 non-classic) and 23 normal adipose tissue specimens. Cytoplasmic and/or nuclear immunohistochemical staining of EGFR and VEGF was analyzed. The angiogenic index was determined by MVC. Cells were counted using the Image J® software. The Statistical Package for the Social Sciences was used for data analysis, adopting a level of significance of 5% for all statistical tests. A statistically significant difference in EGFR immunoexpression (p=0.047), especially, between classic lipomas and normal adipose tissue. There was a significant difference in MVC between non-classic lipomas and normal adipose tissue (p=0.022). In non-classic lipomas, only VEGF immunoexpression showed a significant moderate positive correlation (r=0.607, p=0.01) with MVC. In classic lipomas, the number of EGFR-immunostained adipocytes was directly proportional to the number of VEGF-positive cells, demonstrating a significant moderate positive correlation (r=0.566, p=0.005). The results suggest that EGFR, VEGF, and angiogenesis participate in the development of oral lipomas but are not primarily involved in the growth of these tumors.","PeriodicalId":9211,"journal":{"name":"Brazilian dental journal","volume":"34 2","pages":"113-121"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10208286/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9520402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low concentrations of grape seed extract maintain osteoblast morphology, cell adhesion, and mineralization. 低浓度的葡萄籽提取物维持成骨细胞形态、细胞粘附和矿化。

Brazilian dental journal Pub Date : 2023-03-01 DOI: 10.1590/0103-6440202304987

Maria Carolina Coelho, Viviann Ruocco Vetucci, Roger Rodrigo Fernandes, Paula Katherine Vargas Sanchez, Selma Siessere, Karina Fittipaldi Bombonato-Prado

{"title":"Low concentrations of grape seed extract maintain osteoblast morphology, cell adhesion, and mineralization.","authors":"Maria Carolina Coelho, Viviann Ruocco Vetucci, Roger Rodrigo Fernandes, Paula Katherine Vargas Sanchez, Selma Siessere, Karina Fittipaldi Bombonato-Prado","doi":"10.1590/0103-6440202304987","DOIUrl":"https://doi.org/10.1590/0103-6440202304987","url":null,"abstract":"The increase in life expectancy has led to a higher incidence of osteoporosis, characterized by an imbalance in bone remodeling. Several drugs are used for its treatment, but most promote undesirable side effects. The present investigation evaluated the effects of two low concentrations of grape seed extract (GSE) rich in proanthocyanidins on MC3T3-E1 osteoblastic cells. The cells were cultured in an osteogenic medium and divided into control (C), 0.1 µg/mL GSE (GSE0.1), and 1.0 µg/mL GSE (GSE1.0) groups to evaluate cell morphology, adhesion, and proliferation, in situ alkaline phosphatase (ALP) detection, mineralization and immunolocalization of osteopontin (OPN). The data obtained were analyzed by statistical tests for a significance of 5%. Cell morphology was maintained with both GSE concentrations, whereas cell adhesion significantly increased within three days in all groups. Cell proliferation increased significantly at seven days of culture, followed by a significant decrease in all experimental periods, with no statistical difference among them. In situ detection of ALP and mineralization increased with time, but within each period, no statistical differences among groups were observed. The expression of osteopontin was distributed regularly with more intensity after 24 hours in the GSE0.1 group. After three days, OPN expression was more intense in the control group, followed by GSE0.1 and GSE1.0 groups. Data obtained suggest that low concentrations of GSE do not affect the morphology and may stimulate the functional activity of osteoblastic cells.","PeriodicalId":9211,"journal":{"name":"Brazilian dental journal","volume":"34 2","pages":"97-104"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10208284/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9521780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of automated preparation of long oval-shaped root canals using supplementary instrumentation. 辅助器械自动制备长椭圆形根管的优化研究。

Brazilian dental journal Pub Date : 2023-03-01 DOI: 10.1590/0103-6440202305231

Marco Antonio Castro-Perez, Cleber Keiti Nabeshima, Emmanuel J N L Silva, José Edgar Valdivia, Giulio Gavini, Manoel Eduardo de Lima Machado

{"title":"Optimization of automated preparation of long oval-shaped root canals using supplementary instrumentation.","authors":"Marco Antonio Castro-Perez, Cleber Keiti Nabeshima, Emmanuel J N L Silva, José Edgar Valdivia, Giulio Gavini, Manoel Eduardo de Lima Machado","doi":"10.1590/0103-6440202305231","DOIUrl":"https://doi.org/10.1590/0103-6440202305231","url":null,"abstract":"This study compared the preparation of long oval-shaped root canals using WaveOne Gold and XP-endo Shaper systems, both supplemented or not with manual instrumentation. Twenty-four long oval-shaped canals of mandibular incisors were distributed into two groups according to the instrumentation: WaveOne Gold Primary or XP-endo Shaper systems. All root canals were manually instrumented with a size 25 K-file after automated preparation. The specimens were scanned by using a micro-CT device (17.42 µm) before and after automated preparation and manual instrumentation. The increased surface of the root canal and the percentage of untouched areas were assessed. Both WaveOne Gold and XP-endo Shaper systems increased the root canal surface and had similar untouched areas (p>0.05). Supplementary instrumentation increased the surface of the root canal and decreased the untouched walls (p<0.05). WaveOne Gold and XP-endo Shaper systems provided a similar preparation of long oval-shaped canals and manual instrumentation improved their preparation.","PeriodicalId":9211,"journal":{"name":"Brazilian dental journal","volume":"34 2","pages":"35-40"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10208298/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9513648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the rotary kinematics between actual and set speeds of X-Smart Plus, VDW.Silver and iRoot motors. X-Smart Plus, VDW的实际速度和设定速度之间的旋转运动学评估。银色和iRoot电机。

Brazilian dental journal Pub Date : 2023-03-01 DOI: 10.1590/0103-6440202304953

Renata de Castro Monteiro-Netto, Dieimes Braambati, Rodrigo Arruda-Vasconcelos, Adriana de-Jesus Soares, Marcos Frozoni

{"title":"Evaluation of the rotary kinematics between actual and set speeds of X-Smart Plus, VDW.Silver and iRoot motors.","authors":"Renata de Castro Monteiro-Netto, Dieimes Braambati, Rodrigo Arruda-Vasconcelos, Adriana de-Jesus Soares, Marcos Frozoni","doi":"10.1590/0103-6440202304953","DOIUrl":"https://doi.org/10.1590/0103-6440202304953","url":null,"abstract":"The present study evaluated the actual rotational speed of three different endodontic motors compared to the values provided by the manufacturers. A total of three endodontic motors (X-Smart Plus, VDW.Silver, and iRoot) were tested at 400 rpm and 800 rpm and 2 N/cm2 torque. The kinematics of the devices was recorded by using a custom angle-measuring disc with a 50-mm diameter attached to the handpiece provided by the manufacturer, whereas their movement was captured by a high-speed camera at 2,400 frames per second, 800 x 800 pixel-resolution and distance of 0.3 m from the target object. Statistical analysis was performed at a significance level of 5%. At 400 rpm, the iRoot motor had a value of 17.94 rpm above that indicated by the manufacturer, which was significantly different from those of X-Smart Plus (5.20 rpm below that indicated by the manufacturer) and VDW.Silver (0.62 rpm above that indicated by the manufacturer) motors (P < 0.05). At 800 rpm, the iRoot motor had a value of 51.34 rpm below that indicated by the manufacturer, whereas the X-Smart Plus motor had a value of 13.00 rpm below that indicated by the manufacturer (P > 0.05). The VDW.Silver motor statistically differed from the iRoot and X-Smart Plus ones, showing a value of 1.68 rpm above that indicated by the manufacturer. In conclusion, the X-Smart Plus, VDW.Silver, and iRoot motors showed lower variations in the rotational speed values compared to those reported by their manufacturers. The endodontic motors presented different behaviors between them, with the VDW.Silver motor presents the most accurate values and the iRoot presents the most divergent values.","PeriodicalId":9211,"journal":{"name":"Brazilian dental journal","volume":"34 2","pages":"21-26"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10208292/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9513649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of temperature on the torsional properties of two thermally treated NiTi rotary instruments 温度对两种热处理镍钛旋转仪表扭转性能的影响

Brazilian dental journal Pub Date : 2023-02-01 DOI: 10.11606/d.25.2020.tde-26102021-101827

Roberto Barreto Osaki, C. Bramante, R. Vivan, M. Alcalde, P. Calefi, M. Duarte

{"title":"Influence of temperature on the torsional properties of two thermally treated NiTi rotary instruments","authors":"Roberto Barreto Osaki, C. Bramante, R. Vivan, M. Alcalde, P. Calefi, M. Duarte","doi":"10.11606/d.25.2020.tde-26102021-101827","DOIUrl":"https://doi.org/10.11606/d.25.2020.tde-26102021-101827","url":null,"abstract":"Abstract This study aimed to evaluate the influence of temperature on torsional strength and angular deflection of two experimental NiTi rotary instruments manufactured from Blue and Gold thermal treatments and with identical cross-sections. A total of 40 experimental NiTi instruments 25.06 and with a triangular cross-section and manufactured from Blue and Gold thermal treatments were used (n=20). The torsional test was performed in the 3 mm from the tip of the instrument according to ISO 3630-1. The torsional test evaluated the torsional strength and angular deflection to failure at room temperature (21°C ± 1° C) and body temperature (36°C ±1°C). The fractured surface of each fragment was observed by using scanning electron microscopy (SEM). Data were analyzed using an unpaired t test for inter and intra-group comparison and the level of significance was set at 5%. The results showed that the body temperature did not affect the torsional strength and angular deflection of the instruments when compared with room temperature (P>0.05). However, at body temperature, the Blue NiTi instruments presented significantly lower angular deflection in comparison with Gold NiTi instruments (P<0.05). There was no significant difference regarding the torsional strength of the instruments at body temperature (P>0.05). The temperature did not affect the torsional strength of the instruments manufactured from Blue and Gold technology. However, the Blue NiTi instruments presented significantly lower angular deflection than Gold instruments at 36°C temperature.","PeriodicalId":9211,"journal":{"name":"Brazilian dental journal","volume":"34 1","pages":"12 - 18"},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44976684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Amalgam x Composite Resin: supplies and restorative procedures more performed among Oral Health Teams in Brazil Amalgam x复合树脂：巴西口腔健康团队更多地进行补给品和恢复程序

Brazilian dental journal Pub Date : 2023-01-15 DOI: 10.1590/0103-6440202305003

M. Silva-Júnior, Elis Carolina Pacheco, N. Bastos-Bitencourt, Pollyana Kassia de Oliveira Borges, M. H. Baldani

{"title":"Amalgam x Composite Resin: supplies and restorative procedures more performed among Oral Health Teams in Brazil","authors":"M. Silva-Júnior, Elis Carolina Pacheco, N. Bastos-Bitencourt, Pollyana Kassia de Oliveira Borges, M. H. Baldani","doi":"10.1590/0103-6440202305003","DOIUrl":"https://doi.org/10.1590/0103-6440202305003","url":null,"abstract":"Abstract This study aimed to compare the availability of supplies and amalgam and composite resin restorations among Oral Health Teams (OHT) in Brazilian regions. Secondary data were extracted from Modules I and II of the 1st (2012) and V and VI of the 2nd (2014) and 3rd cycle (2017) of the external evaluation of the National Program for Access and Quality in Primary Care2. The proportions between regions and cycles were compared using the Chi-square test with the z-test adjusted by the Bonferroni method (p<0.05). Among 2012, 2014, and 2017 there was a significant reduction in the proportion of OHT that performed amalgam restorations (87.5%, 89.2%, and 80.2%; p<0.001) and an increase in resin composite (92.5 %, 97.7%, and 99.0%; p<0.001), with the same trend in Brazilian regions (p<0.001). Amalgamator availability decreased among 2012 (99.0%), 2014 (98.4%) and 2017 (85.6%) (p<0.001). Amalgam availability was lower in 2017 (80.1%), compared to 2012 (87.5%) and 2014 (97.5%) (p<0.001). The availability of light curing decreased between 2012 (99.0%), 2014 (98.4%) and 2017 (85.6%) (p<0.001), being less available in the North (95.7%) (p<0.001). The light-curing resin increased between 2012 (94.1%), 2014 (96.6%) and 2017 (97.0%) (p<0.001), with no increase only in the North (p=0.134). While there was a reduction in supplies and amalgam restoration, there was an increase in supplies and resin composite restorations in the period evaluated in all Brazilian regions. However, regional disparities are still evident, with fewer supplies of restorative services in the North region.","PeriodicalId":9211,"journal":{"name":"Brazilian dental journal","volume":"34 1","pages":"75 - 87"},"PeriodicalIF":0.0,"publicationDate":"2023-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46151946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Tomographic, microbiological and histological characterization of secondary apical periodontitis: case series. 继发性根尖牙周炎的断层摄影、微生物学和组织学特征:病例系列。

Brazilian dental journal Pub Date : 2023-01-01 DOI: 10.1590/0103-6440202304590

Marla Mora-Carabalí, Adolfo Contreras, Patricia Rodríguez, Ingrid Zamora, Martha Rodríguez

{"title":"Tomographic, microbiological and histological characterization of secondary apical periodontitis: case series.","authors":"Marla Mora-Carabalí, Adolfo Contreras, Patricia Rodríguez, Ingrid Zamora, Martha Rodríguez","doi":"10.1590/0103-6440202304590","DOIUrl":"https://doi.org/10.1590/0103-6440202304590","url":null,"abstract":"This case series included a tomographic, microbiological, and histopathological description of 15 secondary apical periodontitis (SAP) lesions obtained by apical microsurgery performed in 10 patients to better understand the etiology and pathogenesis of SAP. Preoperative tomographic analyses were performed through Cone beam computerized tomography - Periapical index (CBCT-PAI), and apical microsurgeries were then carried out. The removed apices were used for microbial culturing and for molecular identification using PCR for the detection of 5 strict anaerobic bacteria (P. gingivalis, P. intermedia, P. nigrescens, T. forsythia, and T.denticola) and 3 viruses Herpes simplex viruses (HSV), Cytomegalovirus (CMG) and Epstein-Barr Virus (EBV) by nested PCR. The removed apical lesions were histologically described. Univariate statistical analyses were performed by using STATA MP/16 (StataCorp LLC, College Station, TX, United States). CBCT-PAI analyses revealed PAI 4 and PAI 5 score lesions that involved cortical plate destruction. Eight SAPs were positive by culture, while nine SAP lesions were positive by PCR. Fusobacterium species were the most frequently cultured organisms in 7 SAP lesions, followed by D. pneumosintes in 3. In contrast, by single PCR, T. forsythia and P. nigrescens were detected in 5 lesions, T. denticola in 4 lesions, and P. gingivalis in 2 lesions. Twelve periapical lesions were granulomas, and the remaining three SAP lesions were radicular cysts. In conclusion, this case series study revealed that secondary apical lesions presented tomographic involvement of PAI 3 to 5, and that most SAP lesions were apical granulomas containing anaerobic and facultative microorganisms.","PeriodicalId":9211,"journal":{"name":"Brazilian dental journal","volume":"34 1","pages":"123-132"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10027100/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9149339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Semantic equivalence of the Brazilian version of the Patient Satisfaction Questionnaire (B-PSQ). 巴西版患者满意度问卷(B-PSQ)的语义等价性。

Brazilian dental journal Pub Date : 2023-01-01 DOI: 10.1590/0103-6440202305074

Luisa Gatti-Reis, Renata Negreiros Alvarenga, Lucas Guimarães Abreu, Saul Martins Paiva

{"title":"Semantic equivalence of the Brazilian version of the Patient Satisfaction Questionnaire (B-PSQ).","authors":"Luisa Gatti-Reis, Renata Negreiros Alvarenga, Lucas Guimarães Abreu, Saul Martins Paiva","doi":"10.1590/0103-6440202305074","DOIUrl":"https://doi.org/10.1590/0103-6440202305074","url":null,"abstract":"The Patient Satisfaction Questionnaire (PSQ) is a self-administered instrument to assess adolescent patients' satisfaction with orthodontic treatment. A pre-existing North American instrument was further explored in the Netherlands. Semantic equivalence is part of cross-cultural adaptation and is necessary to achieve a valid and reliable instrument for a specific culture. The present study aimed to evaluate the semantic equivalence of the items, subscales, and overall PSQ between the original English version and the Brazilian Portuguese language version (B-PSQ). The PSQ has 58 items, distributed across 6 subscales: doctor-patient relationship, situational aspects of the clinic, dentofacial improvement, psychosocial improvement, dental function, and a residual category. Semantic equivalence was evaluated according to the following methods: (1) independent translations to Portuguese by two translators, both native in Brazilian Portuguese and fluent in English; (2) an expert committee drafted the first summarized version in Portuguese; (3) two independent back-translations into English by two native English-speaking translators fluent in Portuguese; (4) committee review; (5) committee drafted a summarized version of the back-translations; (6) expert committee drafted the second summarized version in Portuguese; (7) pre-test of the instrument using individual semi-structured interviews with 10 adolescents; (8) review and final version of the B-PSQ. Semantic equivalence between the original and the Brazilian versions of the questionnaire was achieved through diligent and rigorous methods, with effective translation and expert evaluations, incorporating the opinions of the target population.","PeriodicalId":9211,"journal":{"name":"Brazilian dental journal","volume":"34 1","pages":"107-122"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10027093/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9149340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of BlueM® against Streptococcus mutans biofilm and its virulence gene expression. BlueM®抗变形链球菌生物膜及其毒力基因表达的影响。

Brazilian dental journal Pub Date : 2023-01-01 DOI: 10.1590/0103-6440202305133

Veronica Canela Estevam Dos Santos, Patricia Milagros Maquera-Huacho, Maria Júlia Mancim Imbriani, Vivian M Tellaroli Rodrigues Minhaco, Denise M Palomari Spolidorio

{"title":"Effects of BlueM® against Streptococcus mutans biofilm and its virulence gene expression.","authors":"Veronica Canela Estevam Dos Santos, Patricia Milagros Maquera-Huacho, Maria Júlia Mancim Imbriani, Vivian M Tellaroli Rodrigues Minhaco, Denise M Palomari Spolidorio","doi":"10.1590/0103-6440202305133","DOIUrl":"https://doi.org/10.1590/0103-6440202305133","url":null,"abstract":"This study evaluated the antimicrobial capacity of BlueM® mouthwash against the bacterium Streptococcus mutans and its influence on gbpA gene expression as well as its cytotoxic effect on fibroblast cells. BlueM® showed antimicrobial activity, with MIC and MBC values of 0.005% and 0.01%, respectively. The MBIC was 6.25% for S. mutans. CFU count and confocal microscopy revealed significant effect of BlueM® on S. mutans biofilm pre-formed on dentin surfaces. Interestingly, the analysis of gbpA gene expression indicated a decrease in gene expression after 15 min of treatment with BlueM® at a concentration of 25%. Moreover, BlueM® exhibited low levels of cytotoxicity. In conclusion, our results showed the antimicrobial effectiveness of BlueM® against S. mutans, its ability to modulate the expression of the gbpA gene and its low cytotoxicity. This study supports the therapeutic potential of BlueM® as an alternative agent for the control of oral biofilm.","PeriodicalId":9211,"journal":{"name":"Brazilian dental journal","volume":"34 1","pages":"19-28"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10027103/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9149341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Failure behavior of zirconia crowns subjected to air abrasion with different particle sizes. 氧化锆冠在不同粒度空气磨蚀下的失效行为。

Brazilian dental journal Pub Date : 2023-01-01 DOI: 10.1590/0103-6440202304998

João Cézar Mosele, Andressa Restani Oliveira, Gabriela Pizzolatto, Paula Benetti, Marcia Borba

{"title":"Failure behavior of zirconia crowns subjected to air abrasion with different particle sizes.","authors":"João Cézar Mosele, Andressa Restani Oliveira, Gabriela Pizzolatto, Paula Benetti, Marcia Borba","doi":"10.1590/0103-6440202304998","DOIUrl":"10.1590/0103-6440202304998","url":null,"abstract":"This study aimed to investigate the failure behavior of 3 mol.% yttria-stabilized tetragonal zirconia polycrystal (3Y-TZP) prosthetic crowns air-abraded with aluminum oxide (AO) particles of different sizes. Ninety ceramic premolar crowns were produced with 3Y-TZP frameworks veneered with porcelain. Crowns were randomly divided into three groups, according to the size of the air abrasion AO particles (n = 30): (GC) untreated (control); (G53) 53 µm; (G125) 125 µm. Air abrasion was performed with 0.25 mpa pressure, 10-mm distance, for 10 s. Crowns were adhesively cemented to dentin analog abutments. Specimens were loaded in compression to failure, in 37oC distilled water, using a universal testing machine (n = 30). Fractographic analysis was performed using a stereomicroscope and SEM. The roughness of the crown's inner surface was evaluated using an optical profilometer (n = 10). Fracture load data were statistically analyzed with Weibull analysis and roughness data with Kruskal-Wallis (α = 0.05). GC had the lowest characteristic fracture load (L0), while G53 and G125 had higher and statistically similar L0 values. The Weibull modulus (m) was similar among groups. The failure modes observed were catastrophic failure and porcelain chipping. There were no differences between the roughness parameters for the experimental groups (p > 0.05). The size of the AO particles did not affect the fracture load and failure mode of 3Y-TZP crowns. Air abrasion with 53 µm and 125 µm particles resulted in a higher fracture load of ceramic crowns than the untreated group while maintaining their reliability and surface characteristics.","PeriodicalId":9211,"journal":{"name":"Brazilian dental journal","volume":"34 1","pages":"71-79"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10027107/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9153291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0