Effects of BlueM® against Streptococcus mutans biofilm and its virulence gene expression.

Q2 Medicine

Brazilian dental journal Pub Date : 2023-01-01 DOI:10.1590/0103-6440202305133

Veronica Canela Estevam Dos Santos, Patricia Milagros Maquera-Huacho, Maria Júlia Mancim Imbriani, Vivian M Tellaroli Rodrigues Minhaco, Denise M Palomari Spolidorio

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引用次数: 1

Abstract

This study evaluated the antimicrobial capacity of BlueM® mouthwash against the bacterium Streptococcus mutans and its influence on gbpA gene expression as well as its cytotoxic effect on fibroblast cells. BlueM® showed antimicrobial activity, with MIC and MBC values of 0.005% and 0.01%, respectively. The MBIC was 6.25% for S. mutans. CFU count and confocal microscopy revealed significant effect of BlueM® on S. mutans biofilm pre-formed on dentin surfaces. Interestingly, the analysis of gbpA gene expression indicated a decrease in gene expression after 15 min of treatment with BlueM® at a concentration of 25%. Moreover, BlueM® exhibited low levels of cytotoxicity. In conclusion, our results showed the antimicrobial effectiveness of BlueM® against S. mutans, its ability to modulate the expression of the gbpA gene and its low cytotoxicity. This study supports the therapeutic potential of BlueM® as an alternative agent for the control of oral biofilm.

Abstract Image

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BlueM®抗变形链球菌生物膜及其毒力基因表达的影响。

本研究评估了BlueM®漱口水对变形链球菌的抑菌能力、对gbpA基因表达的影响以及对成纤维细胞的细胞毒作用。BlueM®具有抗菌活性，MIC和MBC分别为0.005%和0.01%。变形链球菌的MBIC为6.25%。CFU计数和共聚焦显微镜显示BlueM®对牙本质表面预形成的变形链球菌生物膜有显著的影响。有趣的是，gbpA基因表达分析显示，用浓度为25%的BlueM®治疗15分钟后，基因表达下降。此外，BlueM®表现出低水平的细胞毒性。总之，我们的研究结果显示了BlueM®对变形链球菌的抗菌效果，其调节gbpA基因表达的能力和低细胞毒性。这项研究支持了BlueM®作为控制口腔生物膜的替代药物的治疗潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Brazilian dental journal Dentistry-Dentistry (all)

CiteScore

2.20

自引率

0.00%

发文量

审稿时长

12 weeks

期刊介绍： Brazilian Dental Journal, publishes Full-Length Papers, Short Communications and Case Reports, dealing with dentistry or related disciplines and edited six times a year.