Veronica Canela Estevam Dos Santos, Patricia Milagros Maquera-Huacho, Maria Júlia Mancim Imbriani, Vivian M Tellaroli Rodrigues Minhaco, Denise M Palomari Spolidorio
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Effects of BlueM® against Streptococcus mutans biofilm and its virulence gene expression.
This study evaluated the antimicrobial capacity of BlueM® mouthwash against the bacterium Streptococcus mutans and its influence on gbpA gene expression as well as its cytotoxic effect on fibroblast cells. BlueM® showed antimicrobial activity, with MIC and MBC values of 0.005% and 0.01%, respectively. The MBIC was 6.25% for S. mutans. CFU count and confocal microscopy revealed significant effect of BlueM® on S. mutans biofilm pre-formed on dentin surfaces. Interestingly, the analysis of gbpA gene expression indicated a decrease in gene expression after 15 min of treatment with BlueM® at a concentration of 25%. Moreover, BlueM® exhibited low levels of cytotoxicity. In conclusion, our results showed the antimicrobial effectiveness of BlueM® against S. mutans, its ability to modulate the expression of the gbpA gene and its low cytotoxicity. This study supports the therapeutic potential of BlueM® as an alternative agent for the control of oral biofilm.
期刊介绍：
Brazilian Dental Journal, publishes Full-Length Papers, Short Communications and Case Reports, dealing with dentistry or related disciplines and edited six times a year.
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