Journal of analytical & pharmaceutical research最新文献

{"title":"Antioxidant actions of olive leaf extract (Olea europaea L.) on reactive species scavengers","authors":"M. Melo","doi":"10.15406/JAPLR.2020.09.00354","DOIUrl":"https://doi.org/10.15406/JAPLR.2020.09.00354","url":null,"abstract":"This study aimed to evaluate in vitro antioxidant action of olive leaf extract (Olea europaea L.) by: i) Trolox equivalent antioxidant capacity (TEAC) by ABTS•+, DPPH and Ferric reducing antioxidant power (FRAP) assays; ii) scavenging of superoxide anion (O2•-), hypochlorous acid (HOCl) and nitric oxide (NO), compared to ascorbic acid. Results showed TEAC values as 0.148±0.015, 0.215±0.076 and 0.282±0.023 gram of trolox equivalent per gram of dry extract weight, to respective ABTS•+, DPPH• and FRAP. Olive leaf extract was better antioxidant than ascorbic acid on O2•- scavenging, at concentrations over 50 µg/mL; similar effects on NO scavenging for both was seen and on HOCl inhibition, the extract showed lower antioxidant action than ascorbic acid at all concentrations. Olive leaf extract showed potentiality to be used as antioxidant in biological systems.","PeriodicalId":92063,"journal":{"name":"Journal of analytical & pharmaceutical research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45477380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design, formulation and characterization of oral disintegrating tablets for lamotrigine","authors":"R. Gunda","doi":"10.15406/JAPLR.2020.09.00353","DOIUrl":"https://doi.org/10.15406/JAPLR.2020.09.00353","url":null,"abstract":"Objective: The purpose of present investigation to formulate, characterize the oral dissolving tablets (ODT) for Lamotrigine. Lamotrigine, an antiepileptic agent, belongs to type –II as per Biopharmaceutical Classification System (BCS). Methods: ODT formulations of Lamotrigine were prepared using different quantities of Sodium Starch Glycolate & Crospovidone employed as Super disintegrants by Direct Compression technique. Nine trials were formulated and evaluated for Pharmaceutical Product Performance. Results: Results shows that all the formulations were lie within the acceptance criterion and the In-vitro dissolution profiles were subjected to kinetic modeling. Conclusion: Formulation (F4) containing 35 mg of Sodium Starch Glycolate & 40 mg of Crospovidone was found to be best one among all and also similar to the Marketed product (LAMICTAL-25) (f2=73.17, f1=3.65 & No significant difference, t=0.0218) to marketed product. Formulation (F4) follows First order, Higuchi’s kinetics, mechanism of drug release was found to be Non-Fickian Diffusion (n= 0.554).","PeriodicalId":92063,"journal":{"name":"Journal of analytical & pharmaceutical research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45000240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conductive acrylic pressure-sensitive adhesives","authors":"A. Antosik","doi":"10.15406/japlr.2020.09.00352","DOIUrl":"https://doi.org/10.15406/japlr.2020.09.00352","url":null,"abstract":"patches with transdermal medications) - transdermal drug delivery systems (TDDS), self-adhesive hydrogels and surgical drapes. Abstract The paper presents the results of research on the impact of the amount of copper on acrylic pressure-sensitive adhesives. The effect of filler addition of 1.0–50.0%wt. on useful properties of adhesives was investigated. Increasing the amount of copper powder in the composition decreased adhesion and tack; cohesion and conductivity was increased. Conductive acrylic pressure-sensitive adhesives have been obtained.","PeriodicalId":92063,"journal":{"name":"Journal of analytical & pharmaceutical research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46687095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural antimicrobial and bioactive compounds from Ludwigia parviflora Roxb","authors":"R. Gobalakrishnan, R. Bhuvaneswari, M. Rajkumar","doi":"10.15406/japlr.2020.09.00349","DOIUrl":"https://doi.org/10.15406/japlr.2020.09.00349","url":null,"abstract":"In vitro screening method could offer a preliminary observation which is necessary to select crude plant extract with potentially useful properties for further chemical and pharmacological investigations. The present research work was designed to investigate the natural antimicrobial and bioactive constituents from Ludwigia parviflora (fruit extract). was tested for antimicrobial activity against four bacterial and three fungal pathogens using the well diffusion method with six different solvent extracts of these, ethyl acetate and ethanol extract of L. parviflora showed significant antimicrobial activity against all the tested pathogens. Bioactive constituents, as revealed by quantitative and qualitative. GC-MS analysis identified the presence of ten bioactive compounds. In conclusion, L. parviflora fruit extracts might be to treat diverse of human diseases and it could be responsible for the discovery of novel therapeutic drugs.","PeriodicalId":92063,"journal":{"name":"Journal of analytical & pharmaceutical research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67073535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis, characterization, anticancer activity and biological activities of vanadium complexes of 2-mercapto-5-methyl-benzimidazole as sulphur donor ligand","authors":"S. Shahzadi, Mudassir Jabeen, Saqib Ali, Li-wu Fu, M. Shahid, Matloob Ahmad","doi":"10.15406/japlr.2020.09.00351","DOIUrl":"https://doi.org/10.15406/japlr.2020.09.00351","url":null,"abstract":"Vanadium complexes have been synthesized by the reaction of 2-mercapto-5-methyl- benzimidazole with carbon disulphide and then with different vanadium salts in different M/L ratio. The synthesized vanadium complexes have been characterized by elemental microanalysis (CHN), FT-IR spectroscopy and NMR ( 1 H, 13 C) spectroscopy. Elemental analysis data shows good agreement between found and calculated values. FT-IR spectroscopic data suggests the bidentate nature of the ligand. The results of multinuclear NMR revealed that coordination occurs through NCS 2 moiety. Antibacterial activity data showed that ligand and complexes showed moderate antibacterial and anti-biofilm activity. The cytotoxicity studies showed that vanadium complex (3) is less cytotoxic (2.27%) and complex (1) has maximum cytotoxicity (11.04%). In vitro oxidative DNA damage protection assay showed that synthesized vanadium complexes exhibited plasmid DNA protection by scavenging the oxidation products. Results of in vitro anticancer activity showed that oxoperoxo vanadium complex (2) exhibited significant anticancer activity (IC 50 2.55µM) against H460 MX2 cell line.","PeriodicalId":92063,"journal":{"name":"Journal of analytical & pharmaceutical research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67073584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of the global warming and environmental temperature on the animal’s molecular response and enzymatic activity","authors":"T. Alinejad, S. Bhassu, R. Y. Othman, F. Mustafa","doi":"10.15406/japlr.2020.09.00350","DOIUrl":"https://doi.org/10.15406/japlr.2020.09.00350","url":null,"abstract":"The intergovernmental panel on climate change officially released an important report that addressed the global warming impact on living phenomena on the earth.1 The environmental temperature might have many fluctuations for many creatures throughout their lives. This effect may occur yearly, seasonally, or daily and usually does not remain constant. There is a question to ponder on, and that is that how an organism struggles with long-term or severe temperature changes.2 Initial heat shock research investigates the mechanisms behind the response to critical heat stress by observing the heat shock in Drosophila. In this study, puffs characteristics had been studied in the salivary gland chromosomes of Drosophila.3 Later, it was understood that these studied chromosomal puffs were associated with RNA synthesis and heat shock protein expression. Since 1974 up till now, it is well known that heat shock protein family including (e.g, Hsp100, Hsp90, Hsp70, Hsp60, Hsp40 and small heat shock protein families) are regulated under “heat shock response” mechanisms in almost all organisms.4 The heat shock proteins are grouped by size or categorized by function. They are also famed as molecular chaperones because they are involved in protein folding. It has also been reported that they are preventing the formation of protein accumulation inside the cell.5 Additionally, it has been reported that they have functions in ATP-independent` (small heat shock proteins) ATP-dependent (Hsp60, Hsp70) mechanisms.6 Initial studies revealed that the binding of the heat shock factor (HSF) to cis-regulatory heat shock element (HSE) regions induced heat shock regulatory networks. Furthermore, interaction between Hsp70 with HSF and can cause heat shock auto regulation or block it.7 Researchers have approved that the heat stress response is a complicated mechanism. Research has also confirmed that the heat shock response has a variation in the initiation or termination timing or in the stress intensity. It furthermore depends on different types of Hsps in different organisms. It also may comprise from the induction of many other non Hsps genes.4,8-11","PeriodicalId":92063,"journal":{"name":"Journal of analytical & pharmaceutical research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67073567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CpG methylation and various parameters interaction in myotonic dystrophy type 1","authors":"Ashok Kumar, S. Agarwal, S. Pradhan","doi":"10.15406/japlr.2020.09.00347","DOIUrl":"https://doi.org/10.15406/japlr.2020.09.00347","url":null,"abstract":"Myotonic dystrophy type 1 is a chronic, slowly progressing, inherited multisystemic autosomal-dominant disease, caused by expansion of CTG repeats in DMPK gene. The purpose of the present study was to analyze molecular expansion profiling of CTG repeat, status of CpG methylation at DMPK gene locus, and to established relationship between CpG methylation and CTG repeat expansion size along with other clinical and biochemical parameters. Clinically suspected 21 DM1 subjects, 56 family members and 50 normal individuals were included in this study. Molecular diagnosis of CTG repeat expansion was performed by Myotonic Dystrophy Short PCR (MDSP) and Triplet primed-PCR (TP-PCR) and followed fragment analysis on ABI-310 Genetic Analyser. The CpG methylation was done by bisulphite conversion kit (Cells to CpG TM Bisulphite conversion kit, 4445555) and 7500 Fast RT-PCR. SPSS version 16 and Pearson correlation coefficient were used for statistical analysis. All clinically suspected 21 subjects had CTG repeat expansion. Among 56 family members, 16 were permutated, and 40 were normal for CTG repeat. Our previous findings (Kumar et al, 2016 and 2018) highlighted that pattern of CTG repeats differs according to ethnicity. Among positive DM1 samples (n=21), 13 samples were methylated. CpG methylation was significantly correlated only with CTG repeat expansion. This methylation may affect the disease environment and expression of neighbor gene which is responsible for disease pathogenesis.","PeriodicalId":92063,"journal":{"name":"Journal of analytical & pharmaceutical research","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67073486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional <i>in silico</i> analysis of human tyrosinase and OCA1 associated mutations.","authors":"Milan Patel, Yuri Sergeev","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Oculocutaneous albinism type 1 (OCA1) is an autosomal recessive disorder caused by mutations in the tyrosinase gene. OCA1 exists in two forms: OCA1A and OCA1B. OCA1A is caused by a full loss of the human tyrosinase protein (Tyr), leading to an absence of pigment in skin, hair, and eyes, while OCA1B has reduced Tyr catalytic activity and pigment. The current understanding of the disease is hampered by the absence of information regarding the alterations of protein structure and the effects leading to either form of OCA1. Here, we used computational methods to find a general mechanism for establishing this link. Tyr and mutant variants were built through homology modeling, glycosylated <i>in silico</i>, minimized, and simulated using 100 ns molecular dynamics in water. For OCA1B mutants, cavity size is linked to ΔΔG values for mutants, suggesting that partial loss of Tyr is associated with the destabilizing effect of the EGF-like domain movement. In OCA1A, active site mutation simulations indicate that the absence of O₂ leads to protein instability. OCA1B mutants are described in severity by the size of the cavity within the EGF-Tyr interface, while active site OCA1A mutants are unable to fully coordinate copper, leading to an absence of O₂ and Tyr instability. In patients with known genotypes, free energy changes may help identify the severity of the disease by assessing either the allosteric effect of the EGF-Tyr cavity in OCA1B or the active site instability in OCA1A.</p>","PeriodicalId":92063,"journal":{"name":"Journal of analytical & pharmaceutical research","volume":"9 3","pages":"81-89"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7808255/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38829611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of antioxidant properties of secondary metabolites in aqueous extracts of Ficus thonningii blume tested on wistar rats","authors":"C. Fokunang, Jessica Ketchemen Pougoue, E. T. Fokunang, E. B. Joseph, J. Ngoupayo, B. Njinkio, James Anjeck Mbah Berinyuy, John Fohnboh Dobgima","doi":"10.15406/japlr.2020.09.00348","DOIUrl":"https://doi.org/10.15406/japlr.2020.09.00348","url":null,"abstract":"Treatment of this disease requires in most cases a combination of several molecules with specific mechanisms of action. This treatment has 4 goals: relieve pain, accelerate healing, prevent complications and reduce the frequency of relapses. But while effective, treatment using conventional medicines is not usually well attended by patients.1 The reasons included their high cost and low availability to a large majority of the population especially those living in rural areas. In many developing countries, the health infrastructure is poor and a large majority of the population, mainly rural, has no access to primary health care and modern medicines. These patients use the resources of traditional herbal medicine as an alternative treatment.","PeriodicalId":92063,"journal":{"name":"Journal of analytical & pharmaceutical research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67073524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of water on ethanolic extraction yield of Azadirachta indica leaf","authors":"Cleidenely Evangelista Franco Borges, E. U. Bucek, Mariângela Terra Branco Camargos, J. Finzer","doi":"10.15406/JAPLR.2019.08.00315","DOIUrl":"https://doi.org/10.15406/JAPLR.2019.08.00315","url":null,"abstract":"","PeriodicalId":92063,"journal":{"name":"Journal of analytical & pharmaceutical research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45469092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}