Journal of analytical & pharmaceutical research最新文献

Effects of paraquat dichloride on adult male wistar rat. an approach in the toxicity of body weights and hematological tissues 百草枯二氯对成年雄性wistar大鼠的影响。体重和血液组织毒性的一种方法

Journal of analytical & pharmaceutical research Pub Date : 2022-01-21 DOI: 10.15406/japlr.2022.11.00394

G. Edo

{"title":"Effects of paraquat dichloride on adult male wistar rat. an approach in the toxicity of body weights and hematological tissues","authors":"G. Edo","doi":"10.15406/japlr.2022.11.00394","DOIUrl":"https://doi.org/10.15406/japlr.2022.11.00394","url":null,"abstract":"The main objective of the experiment is aimed to examine the impact of paraquat dichloride on the body weights of adult male Wistar rats as well as in their haematological tissues. The total number of male Wistar rats was 48 and was divided into 4 groups of 12 each. The 1st group (Test 1) served as control. The other three groups served as experimental groups in which the paraquat dichloride was administered. The experimental groups which are 2nd group (Test 2), 3rd group (Test 3) and 4th group (Test 4) received doses of paraquat dichloride of 10 mg/kg b.wt/per oral/day, 20 mg/kg b.wt/per oral/day and 40 mg/kg b.wt/per oral/day respectively. The experiment was carried out for a time duration of 42 days. The mean values of body weights, erythrocyte indices, total erythrocyte count (TEC), total leucocyte count (TLC) and Hb concentrations were significantly (P<0.05) decreased in Test 2, 3 and 4 rats, excluding packed cell volume (PCV), which in comparison to the control groups. Outcomes and observations from hematological parameters and bone marrow revealed the toxicity of paraquat dichloride to hematological tissues resulting in anemia and bone marrow hypoplasia which eventually leads to failure of the bone marrow and extensively hematopoietic system of adult male Wistar rats following long term exposure and high concentration.","PeriodicalId":92063,"journal":{"name":"Journal of analytical & pharmaceutical research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44624485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Whole genome and transcriptome analysis of genetic determinants associated with cyclophosphamide in vitro in pediatric acute leukemias 体外儿科急性白血病中与环磷酰胺相关的遗传决定因素的全基因组和转录组分析

Journal of analytical & pharmaceutical research Pub Date : 2021-12-03 DOI: 10.15406/japlr.2021.10.00389

Joanna Szczepanek, Sylwia Górka, K. Domagalski, Joanna Dejewska, M. Pogorzała, J. Styczyński, A. Tretyn

{"title":"Whole genome and transcriptome analysis of genetic determinants associated with cyclophosphamide in vitro in pediatric acute leukemias","authors":"Joanna Szczepanek, Sylwia Górka, K. Domagalski, Joanna Dejewska, M. Pogorzała, J. Styczyński, A. Tretyn","doi":"10.15406/japlr.2021.10.00389","DOIUrl":"https://doi.org/10.15406/japlr.2021.10.00389","url":null,"abstract":"Introduction: The main objective was to implement the determinations of the ex vivo resistance to cyclophosphamide and to identify the genetic profile for pediatric patients with acute leukemias. Methods: In order to determine the ex vivo drug resistance profile, MTT cytotoxicity assay was performed on mononuclear cells. Gene expression profiles were prepared on the basis of cRNA hybridization to oligonucleotide arrays of the human genome (Affymetrix). We performed also array-based comparative genomic hybridization using a SurePrint G3 Human CGH Microarray. Data was analyzed by bioinformatics tools. Verification of the relative expression level of 20 genes was carried out by qRT- PCR. Results: We observed a multitude of differentially expressed genes, e.g. ANXA1 (FC=3,04), BCL2A1 (FC=2,69), SERPINA1 (FC=2,12), DHRS7 (FC=2,13), PCDH9 (FC=- 4,58), TTC28 (FC=-2,25) and DUSP1 (FC=-2,91). The expression of genes that code for inflammation mediated by chemokine and cytokine signaling, Wnt, angiogenesis and integrin signaling and T cell activation pathways genes affect the sensitivity of leukemic blasts to cyclophosphamide. Transcriptome level changes are associated with chromosomal aberrations, especially located on chromosomes 8, 10, 14, 15, 16 and 22. Conclusion: Our work delineated genes with differentiated expression and recurrent copy number changes, and revealed novel amplified loci and frequent deletions in resistant to cyclophosphamide cells, which may guide future work aimed at identifying the relevant target genes. In particular, deletion seems to be a frequent mechanism of IFIT3 gene inactivation. ANXA1, SERPINA1, TCF7 and BCL2A1 may also be included among the candidate genes of resistance (Ontological analysis).","PeriodicalId":92063,"journal":{"name":"Journal of analytical & pharmaceutical research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47182575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antioxidant activity and resveratrol content in wines the national origin 国产葡萄酒的抗氧化活性和白藜芦醇含量

Journal of analytical & pharmaceutical research Pub Date : 2021-11-22 DOI: 10.15406/japlr.2021.10.00388

Callisaya A Juan Carlos, Arteaga SM del Carmen, Taquichiri T Marco A, Gutiérrez Barea Pastor, Paco Sarzuri Joel, Solano Iván T, Díaz de Oropeza Rosario, Vargas M Mariela

{"title":"Antioxidant activity and resveratrol content in wines the national origin","authors":"Callisaya A Juan Carlos, Arteaga SM del Carmen, Taquichiri T Marco A, Gutiérrez Barea Pastor, Paco Sarzuri Joel, Solano Iván T, Díaz de Oropeza Rosario, Vargas M Mariela","doi":"10.15406/japlr.2021.10.00388","DOIUrl":"https://doi.org/10.15406/japlr.2021.10.00388","url":null,"abstract":"Wines are an important source of resveratrol and polyphenols, in this sense this work has focused on the determination of antioxidant activity and resveratrol content in wines of origin. The different wines analyzed exhibited strong antioxidant activity for the ABTS and DPPH tests, which are in the ranges of 2.123 to 25.097 [µmol/ml] and 2.348 to 17.138 [µmol/ml] for red wines respectively, while for white wines the ranges they are 0.793 to 2.604 [µmol/ml] and 0.419 to 3.07 [µmol/ml] respectively. Regarding the quantification and presence of trans-resveratrol, this was confirmed in most of the wines analyzed, with concentrations ranging between 0.77 and 3.25 [ppm] in red wines. As for white wines, these oscillate in the range of 0.18 to 0.69 [ppm]. Analyzing and comparing the results obtained in wines of origin, we observe that red wines have a good content of resveratrol and that in turn comparing results of other authors, we observe that the content of resveratrol in wines of origin is within the bibliographic range, both for red and white wines.","PeriodicalId":92063,"journal":{"name":"Journal of analytical & pharmaceutical research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47902305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quality assurance and quality control. current furtherances and hereafter point of view 质量保证和质量控制。当前的进一步发展和以后的观点

Journal of analytical & pharmaceutical research Pub Date : 2021-10-21 DOI: 10.15406/japlr.2021.10.00386

Gudisa Bereda

{"title":"Quality assurance and quality control. current furtherances and hereafter point of view","authors":"Gudisa Bereda","doi":"10.15406/japlr.2021.10.00386","DOIUrl":"https://doi.org/10.15406/japlr.2021.10.00386","url":null,"abstract":"Quality assurance can be delineated as \"section of quality management concentrated on furnishing confidence that quality must-have will be performed.\" The confidence delivered by quality assurance is twice intrinsically to management and extrinsically to clients, government agencies, regulators, certifiers, and third parties. An alternate delineation is \"entire the aimed and organized activities accomplishment within the quality method that can be substantiated to hand over confidence that a commodity or service will perform must-have for quality. Quality assurance is across-the-board and does not have to do with the specific must-have of the product being developed. QA hobbies and responsibilities fill in practically entire of the fantabulous technique in lone fashion or distinctive, while QC is a subset of the QA hobbies. Also, constituents in the fantabulous technique might not be concretely substituted by QA/QC hobbies and responsibilities but perhaps enclose QA and QC. Quality control is a procedure which contemplates on performing the quality demand. Quality control intend to distinguish (and dead-on) imperfection in the finished product. Quality control, in consequence, is a reactive procedure. Quality control can be delineated as \"section of quality management emphasized on furnishing quality must-have.","PeriodicalId":92063,"journal":{"name":"Journal of analytical & pharmaceutical research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43278054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A novel practical approach to calculate measurement uncertainty in clinical pathology laboratories using quality control data with the use of biological variation where applicable 一种新的实用方法来计算测量不确定度在临床病理实验室使用质量控制数据与使用生物变异在适用的地方

Journal of analytical & pharmaceutical research Pub Date : 2021-10-20 DOI: 10.15406/japlr.2021.10.00385

A. Mina, Shanmugam Banukumar, Santiago Vazquez

{"title":"A novel practical approach to calculate measurement uncertainty in clinical pathology laboratories using quality control data with the use of biological variation where applicable","authors":"A. Mina, Shanmugam Banukumar, Santiago Vazquez","doi":"10.15406/japlr.2021.10.00385","DOIUrl":"https://doi.org/10.15406/japlr.2021.10.00385","url":null,"abstract":"Background: Measurement Uncertainty (MU) can assist the interpretation and comparison of the laboratory results against international diagnostic protocols, facilitate a reduction in health care costs and also help protect laboratories against legal challenges. Determination of MU for quantitative testing in clinical pathology laboratories is also a requirement for ISO 15189. Methods: A practical and simple to use statistical model has been designed to make use of data readily available in a clinical laboratory to assess and establish MU for quantitative assays based on internal quality control data to calculate Random Error and external quality assurance scheme results to calculate Systematic Error. The model explained in this article has also been compared and verified against quality specifications based on Biological Variation. Results: Examples that explain and detail MU calculations for the proposed model are given where different components of MU are calculated with tabulated results. Conclusions: The designed model is cost-effective because it utilises readily available data in a clinical pathology laboratory. Data obtained from internal quality control programs and external quality assurance schemes are used to calculate the MU using a practical and convenient approach that will not require resources beyond what is available. Such information can additionally be useful not only in establishing limits for MU to satisfy ISO 15189 but also in selecting and/or improving methods and instruments in use. MU can as well play an important role in reducing health care costs as shown by examples in the article.","PeriodicalId":92063,"journal":{"name":"Journal of analytical & pharmaceutical research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47861538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of viscosity in binary mixtures of dimethyl sulphoxide at 298.15K 298.15K时二甲基亚砜二元混合物粘度的评价

Journal of analytical & pharmaceutical research Pub Date : 2021-09-30 DOI: 10.15406/japlr.2021.10.00383

S. Baluja

引用次数: 0

Natural bioactive compounds and antimicrobial potential of Pavetta indica L. 印度帕韦塔的天然生物活性化合物及其抗菌潜力。

Journal of analytical & pharmaceutical research Pub Date : 2021-08-27 DOI: 10.15406/japlr.2021.10.00381

Gobalakrishnan R, B. R

{"title":"Natural bioactive compounds and antimicrobial potential of Pavetta indica L.","authors":"Gobalakrishnan R, B. R","doi":"10.15406/japlr.2021.10.00381","DOIUrl":"https://doi.org/10.15406/japlr.2021.10.00381","url":null,"abstract":"Terrestrial plants have opened up a new perspective for pharmaceutical research and development of novel antimicrobial agents for the treatment of microbial diseases. Therefore, it is necessary to start an urgent need to screen the plants for novel bioactive compounds. In this context, present study was carried out to screen phytochemical constituents from leaves of Pavetta indica L., and its antimicrobial potential. P. indica leaves possess Chlorophyll a (1.21 ±0.05 mg g-1), Chlorophyll b (1.61 ±0.09 mg g-1), total chlorophyll (2.82 ±0.13 mg g-1), carotenoids (0.57 ±0.06 mg g-1), total soluble sugars (30.08 ±0.79 mg g-1), total soluble starch (13.54 ± 0.72), total soluble proteins (47.81 ± 2.82 mg g-1), total free amino acids (5.77 ± 0.69 mg g-1), total phenol (7.67 ± 0.72 mg g-1), hydroxyl phenols (5.74 ± 0.29 mg g-1) and lipids (53.53 ± 2.50 mg g-1) while alkaloids, tannins, steroids, phenolic compounds, saponins, triterpenoids and cardiac glycosides were also present in the leaves of P. indica. Absolute ethanolic extract of P. indica showed significant antimicrobial activity against all the tested pathogens, followed by aqueous extract. In concluded that, P. indica leaves crude extracts have wide range of bioactive compounds and which might be led to potential antimicrobial activity. They may lend to developing novel therapeutic agent to different infectious diseases.","PeriodicalId":92063,"journal":{"name":"Journal of analytical & pharmaceutical research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46364240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of the addition of selected silicon fillers on Si- PSA shrinkage 硅填料对硅-PSA收缩性能的影响

Journal of analytical & pharmaceutical research Pub Date : 2021-08-25 DOI: 10.15406/japlr.2021.10.00380

A. Antosik, Nataniel Adrian Antosik

引用次数: 1

A novel acyl hydrazone schiff’s bases of benzimidazole-2-thiol 苯并咪唑-2-硫醇席夫碱的新型酰基腙

Journal of analytical & pharmaceutical research Pub Date : 2021-08-18 DOI: 10.15406/japlr.2021.10.00379

A. Hassan, N. Khan

引用次数: 1

Mitochondrial dysfunctions and neurodegenerative diseases: a mini-review 线粒体功能障碍和神经退行性疾病:一个小型综述

Journal of analytical & pharmaceutical research Pub Date : 2021-08-16 DOI: 10.15406/japlr.2021.10.00378

Ratan Kumari, Nikhila Shekhar, S. Tyagi, A. Thakur

引用次数: 1