Journal of analytical & pharmaceutical research最新文献_第3页

Formulation development of topical antibacterial lotion with theobroma cacao pod husk ash extract for treatment of shaving bumps 可可豆荚灰提取物局部抗菌洗剂治疗剃须疙瘩的配方研制

Journal of analytical & pharmaceutical research Pub Date : 2021-02-11 DOI: 10.15406/JAPLR.2021.10.00362

Hannah Olufunmilola Akinrotohun, A. O. Oyedele, O. Igbeneghu, L. O. Orafidiya

{"title":"Formulation development of topical antibacterial lotion with theobroma cacao pod husk ash extract for treatment of shaving bumps","authors":"Hannah Olufunmilola Akinrotohun, A. O. Oyedele, O. Igbeneghu, L. O. Orafidiya","doi":"10.15406/JAPLR.2021.10.00362","DOIUrl":"https://doi.org/10.15406/JAPLR.2021.10.00362","url":null,"abstract":"The aims of this study were to determine the emulsifying properties of Theobroma cacao pod husk ash (CPHA) methanolic extract combined with shea butter and explore the antibacterial activities and physicochemical characteristics of resulting emulsions toward the development of a topical antibacterial lotion formulation for shaving bumps treatment. The ash resulting from combustion of pod husks of freshly harvested ripe cocoa fruits was extracted with methanol and the extract evaporated to dryness. Shea butter was also extracted by traditional method from kernels from the shea tree. These natural-source materials were combined with pharmaceutical ingredients (buffer, viscosity enhancer, preservative) to develop fluid emulsion formulations. Stability characteristics (droplet size, viscosity, creaming, and pH) of the formulations were evaluated as well as their antibacterial activities against microorganisms isolated from after-shave bump swabs of adult male volunteers and against reference organisms; in order to select product(s) of best qualities suitable as shaving bumps medication. The prototype formulations exhibited suitable physicochemical properties and demonstrated inhibitory activities against several isolated shaving bump microbes and the reference organisms namely, Staphylococcus aureus, Bacillus subtilis and Pseudomonas aeruginosa. Two formulations were finally selected as having physicochemical and antibacterial qualities most suitable for shaving bumps therapy, which contained shea butter (20%), citrate buffer (5%), and parabens (0.3%), prepared using 5% CPHA extract solution with and without methyl cellulose (2%), respectively. The novel shea butter-incorporated emulsion-lotion formulations of CPHA extract provide a useful therapeutic option of topical medication for the treatment of shaving bumps in men.","PeriodicalId":92063,"journal":{"name":"Journal of analytical & pharmaceutical research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43597882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination and quantitation of benzofuran, Indole and piperazine containing selective serotonin reuptake inhibitor vilazodone hydrochloride in human plasma by LC-ESI-MS/MS with an application to pharmacokinetic study under the frame work of bioequivalence study LC-ESI-MS/MS法测定人血浆中苯并呋喃、吲哚和哌嗪选择性5-羟色胺再摄取抑制剂盐酸维拉唑酮的含量及其在生物等效性框架下的药代动力学研究中的应用

Journal of analytical & pharmaceutical research Pub Date : 2021-02-08 DOI: 10.15406/japlr.2021.10.00361

B. Ghosh, P. Mandal, S. Chakraborty, Rakesh Bera, C. Saha, S. Poddar, S. Barma, J. Khanam, Sanmoy Karmakar, T. Pal

{"title":"Determination and quantitation of benzofuran, Indole and piperazine containing selective serotonin reuptake inhibitor vilazodone hydrochloride in human plasma by LC-ESI-MS/MS with an application to pharmacokinetic study under the frame work of bioequivalence study","authors":"B. Ghosh, P. Mandal, S. Chakraborty, Rakesh Bera, C. Saha, S. Poddar, S. Barma, J. Khanam, Sanmoy Karmakar, T. Pal","doi":"10.15406/japlr.2021.10.00361","DOIUrl":"https://doi.org/10.15406/japlr.2021.10.00361","url":null,"abstract":"Aim and objectives: Among the various SSRI and 5-HT-1A, partial agonist vilazodone is one of them. It has antidepressant and anti-anxiety activities. This method's main aim and the objective was to develop and validate a bio-analytical method of Vilazodone in human plasma by LC-MS/MS (API-4000) and its application to estimate pharmacokinetics. Method: For reporting this investigation, Analyst software, 1.6.3 used. The mobile phase was acetonitrile with 0.1% formic acid as an organic solvent and Milli Q water with 10Mm ammonium acetate and 0.1% formic acid using the gradation method 7.0min run time. The calibration standard concentrations were 1.0 to 64 ng/ml. Plasma precipitation was by protein precipitation technique. Result: The accuracy of calibration concentrations of Vilazodone was 93.5-104.39% and stability study showed 96.41-106.71%, 94.77-96.36%, 92.22-101.38%, 94.15-98.47%, 93.95-95.75% remaining for freeze-thaw, short term, long term, benchtop and autosampler stability respectively. Recovery was to be 98.10-98.99%; the matrix factor was 0.94-0.96. The maximum plasma concentration of reference preparation was 13.445±2.842ng/ml (Cmax) at a time 6.792±0.846hr. (Tmax). The maximum plasma concentration of test preparation was 13.218±3.231ng/ml (Cmax) at a time 6.958±0.793hr (Tmax) The relative bioavailability of the test preparation was to be 94.66 % of that of the reference preparation. Conclusion: The present investigation was highly selective, sensitive, reproducible, low matrix effect, high recovery and low time-consuming method. It was validated as per USFDA and EMA guideline and successfully used in comparative pharmacokinetics.","PeriodicalId":92063,"journal":{"name":"Journal of analytical & pharmaceutical research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45031119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Reporting and interpreting the numerical results of a vaccine trial: a practical guide for students and health care professionals 报告和解释疫苗试验的数值结果:给学生和卫生保健专业人员的实用指南

Journal of analytical & pharmaceutical research Pub Date : 2021-01-01 DOI: 10.15406/japlr.2021.10.00370

A. Manfrin, Miland Joshi

引用次数: 0

Homology modeling and global computational mutagenesis of human myosin VIIa. 人肌球蛋白via的同源性建模与全局计算诱变。

Journal of analytical & pharmaceutical research Pub Date : 2021-01-01 Epub Date: 2021-03-04 DOI: 10.15406/japlr.2021.10.00364

Annapurna Kuppa, Yuri V Sergeev

{"title":"Homology modeling and global computational mutagenesis of human myosin VIIa.","authors":"Annapurna Kuppa, Yuri V Sergeev","doi":"10.15406/japlr.2021.10.00364","DOIUrl":"10.15406/japlr.2021.10.00364","url":null,"abstract":"<p><p>Usher syndrome type 1B (USH1B) is a genetic disorder caused by mutations in the unconventional Myosin VIIa (MYO7A) protein. USH1B is characterized by hearing loss due to abnormalities in the inner ear and vision loss due to retinitis pigmentosa. Here, we present the model of human MYO7A homodimer, built using homology modeling, and refined using 5 ns molecular dynamics in water. Global computational mutagenesis was applied to evaluate the effect of missense mutations that are critical for maintaining protein structure and stability of MYO7A in inherited eye disease. We found that 43.26% (77 out of 178 in HGMD) and 41.9% (221 out of 528 in ClinVar) of the disease-related missense mutations were associated with higher protein structure destabilizing effects. Overall, most mutations destabilizing the MYO7A protein were found to associate with USH1 and USH1B. Particularly, motor domain and MyTH4 domains were found to be most susceptible to mutations causing the USH1B phenotype. Our work contributes to the understanding of inherited disease from the atomic level of protein structure and analysis of the impact of genetic mutations on protein stability and genotype-to-phenotype relationships in human disease.</p>","PeriodicalId":92063,"journal":{"name":"Journal of analytical & pharmaceutical research","volume":"10 1","pages":"41-48"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8059627/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38901018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Miscibility and thermal properties of poly(vinylpyrrolidone-vinyl acetate)/lamivudine and tenofovir binary blends prepared by hot melt extrusion 热熔挤压法制备聚乙烯吡咯烷酮-醋酸乙烯酯/拉米夫定-替诺福韦二元共混物的混相及热性能

Journal of analytical & pharmaceutical research Pub Date : 2021-01-01 DOI: 10.15406/japlr.2021.10.00373

M. Dias, Lucyenne S Barbosa, Rodrigo B Anjos, Adriana PD Baptista, Fabio ML Dantas

{"title":"Miscibility and thermal properties of poly(vinylpyrrolidone-vinyl acetate)/lamivudine and tenofovir binary blends prepared by hot melt extrusion","authors":"M. Dias, Lucyenne S Barbosa, Rodrigo B Anjos, Adriana PD Baptista, Fabio ML Dantas","doi":"10.15406/japlr.2021.10.00373","DOIUrl":"https://doi.org/10.15406/japlr.2021.10.00373","url":null,"abstract":"Preparation of amorphous solid dispersions (ASD) by hot melt extrusion (HME) of poly(vinylpyrrolidone-vinyl acetate) (Kollidon) and the active pharmaceutical ingredients (API) Lamivudine (3TC) and Tenofovir Disoproxyl Fumarate (TDF) was investigated aiming to study their miscibility and thermal behavior. These two drugs are currently used as drugs in first line treatment of patients with Acquired Immunodeficiency Syndrome (AIDS). In order to predetermine parameters for extrusion and the maximum concentration of API to be used without any recrystallization, binary blends were first processed in the mixing chamber at 130°C using a roller type rotor at 10, 20 and 30 rpm for 7 min, giving rise to ASD of both API, at least up to 20 wt% of the drug. Both 3TC and TDF were then extruded individually with Kollidon in a single screw extruder. HME produced ASD with high concentration of both Kollidon/API combinations, being part of the API soluble in the polymer matrix. From HME, Kollidon/3TC forms ASD in concentration up to 50 wt%, but from 30 wt% 3TC, the ASD has no time stability, showing recrystallization in less than 5 months, while the Kollidon/TDF system forms ASD up to 30 wt% of TDF, but it has aging stability inferior to 4 months.","PeriodicalId":92063,"journal":{"name":"Journal of analytical & pharmaceutical research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67073181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis and simulation of new cyclodextrin derivatives breaker for Aß42 新型环糊精衍生物Aß42破胶剂的合成与模拟

Journal of analytical & pharmaceutical research Pub Date : 2020-11-30 DOI: 10.15406/JAPLR.2020.09.00360

Assia Keniche, K. Taleb

引用次数: 0

Phytochemistry and method preparation of some medicinal plants used to treat asthma-review 几种治疗哮喘的药用植物化学及方法制备综述

Journal of analytical & pharmaceutical research Pub Date : 2020-10-30 DOI: 10.15406/JAPLR.2020.09.00359

Bihonegn Sisay, E. Debebe, Asfaw Meresa, Worku Gemechu, T. Kasahun, Frehiwot Teka, Tigist Abera, Samson Taye, Kidus Tesfay, Samuel T. Tessema, Sileshi Degu

{"title":"Phytochemistry and method preparation of some medicinal plants used to treat asthma-review","authors":"Bihonegn Sisay, E. Debebe, Asfaw Meresa, Worku Gemechu, T. Kasahun, Frehiwot Teka, Tigist Abera, Samson Taye, Kidus Tesfay, Samuel T. Tessema, Sileshi Degu","doi":"10.15406/JAPLR.2020.09.00359","DOIUrl":"https://doi.org/10.15406/JAPLR.2020.09.00359","url":null,"abstract":"Asthma is one of the most common chronic diseases in the present day in which its incidence and severity are increasing. It is highly prevalent global disease; affecting approximately 300 million individuals worldwide. Different medicinal plants have been discovered and used in traditional medicine practices for the treatment of asthma in different part of the world since prehistoric times. The aim of this review article is to explore and compile the ethno medicinal uses, phytochemistry and method of preparation of commonly used medicinal plants for the management of asthma in Ethiopia. A comprehensive and systematic web-based search of full length experimental, ethno-botanical and ethno medicinal survey articles from scientific database like Pub Med, Science Direct, Web of Science and Google Scholar, were undertaken using key words: asthma, medicinal plants, ethno-botanical, herbal, phytochemistry so as to document this review article. About 23 medicinal plants like Papilionaceae, Caricaceae, Moringaceae, Liliaceae, Asteraceae etc. were found among the most common used medicinal plants in the society for the treatment of asthma. A good number of contemporary medicines have evolved from traditional medicines thus in the present circumstances, our traditional system must be given an objective and critical examination. Further research must be carried out to determine the fraction(s)/compounds that are mostly responsible for the observed anti-asthmatic activity as this will lead to drug development. As well, the potency of plants used in combination as beside being used individually should be further researched. It is recommended that further research is needed to anti-asthmatic plants and standardization of herbal formulations/preparations and to determine their side effect, efficacy, safety etc.","PeriodicalId":92063,"journal":{"name":"Journal of analytical & pharmaceutical research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47157963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Dental practice management and research during the COVID-19 pandemic- An overview COVID-19大流行期间的牙科实践管理和研究-概述

Journal of analytical & pharmaceutical research Pub Date : 2020-09-10 DOI: 10.15406/japlr.2020.09.00357

Vamsi Lavu, Sindhu Saeralaathan, S. K. Balaji

引用次数: 1

Production of biodiesel by enzymatic catalysis from the castor oil: an analytical chemical study 蓖麻油酶催化生产生物柴油的分析化学研究

Journal of analytical & pharmaceutical research Pub Date : 2020-08-07 DOI: 10.15406/JAPLR.2020.09.00355

João Carlos Assunção

{"title":"Production of biodiesel by enzymatic catalysis from the castor oil: an analytical chemical study","authors":"João Carlos Assunção","doi":"10.15406/JAPLR.2020.09.00355","DOIUrl":"https://doi.org/10.15406/JAPLR.2020.09.00355","url":null,"abstract":"The study aimed at the development of a method for obtaining biodiesel by transesterification by via methylic from castor oil using biocatalysts. Seven varieties of lipase (PPL, Candida rugosa, OS cepacea, OS cepacea immobilized on diatomaceous earth, Novozyme 435, Lipozyme and Lipozyme RM) and seven enzymatic sources from a vegetable origin (latex of the species Ficus benjamina, Manihot glaziovii Muell. Arg., Calotropis procera, Jatropha curcas L., Ficus pumila, Euphorbia tirucalli L.) were tested. Enzymatic sources of plant origin were submitted, initially, the hydrolytic activity tests to evaluate their potential as biocatalysts and, from the results obtained, two samples were selected for the experiments further synthesis of biodiesel. Validation of the method of production of biodiesel evaluated linearity, reproducibility (precision), recovery (accuracy) and the limits of detection and quantification. Among the sources of enzymes used in the synthesis of biodiesel presented the best result was the commercial enzyme Novozym 435 with 55.33% conversion yield of biodiesel in initial tests. Were conducted new tests for optimization of reactional parameters co-solvent, alcohol: oil ratio, amount of enzyme and solvent for recovery of the enzyme, which enabled a maximum yield of biodiesel to 98.84%.","PeriodicalId":92063,"journal":{"name":"Journal of analytical & pharmaceutical research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43377209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Functional in silico analysis of human tyrosinase and OCA1 associated mutations 人酪氨酸酶和OCA1相关突变的功能性计算机分析

Journal of analytical & pharmaceutical research Pub Date : 2020-06-10 DOI: 10.15406/JAPLR.2020.09.00356

Milan Patel, Y. Sergeev

{"title":"Functional in silico analysis of human tyrosinase and OCA1 associated mutations","authors":"Milan Patel, Y. Sergeev","doi":"10.15406/JAPLR.2020.09.00356","DOIUrl":"https://doi.org/10.15406/JAPLR.2020.09.00356","url":null,"abstract":"Oculocutaneous albinism type 1 (OCA1) is an autosomal recessive disorder caused by mutations in the tyrosinase gene. OCA1 exists in two forms: OCA1A and OCA1B. OCA1A is caused by a full loss of the human tyrosinase protein (Tyr), leading to an absence of pigment in skin, hair, and eyes, while OCA1B has reduced Tyr catalytic activity and pigment. The current understanding of the disease is hampered by the absence of information regarding the alterations of protein structure and the effects leading to either form of OCA1. Here, we used computational methods to find a general mechanism for establishing this link. Tyr and mutant variants were built through homology modeling, glycosylated in silico, minimized, and simulated using 100 ns molecular dynamics in water. For OCA1B mutants, cavity size is linked to ΔΔG values for mutants, suggesting that partial loss of Tyr is associated with the destabilizing effect of the EGF-like domain movement. In OCA1A, active site mutation simulations indicate that the absence of O2 leads to protein instability. OCA1B mutants are described in severity by the size of the cavity within the EGF–Tyr interface, while active site OCA1A mutants are unable to fully coordinate copper, leading to an absence of O2 and Tyr instability. In patients with known genotypes, free energy changes may help identify the severity of the disease by assessing either the allosteric effect of the EGF-Tyr cavity in OCA1B or the active site instability in OCA1A.","PeriodicalId":92063,"journal":{"name":"Journal of analytical & pharmaceutical research","volume":"9 1","pages":"81 - 89"},"PeriodicalIF":0.0,"publicationDate":"2020-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43188904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6