Roux's archives of developmental biology : the official organ of the EDBO最新文献

{"title":"Enhancer-trap detection of expression patterns corresponding to the polar coordinate system in the imaginal discs of Drosophila melanogaster.","authors":"Satoshi Goto,&nbsp;Teiichi Tanimura,&nbsp;Yoshiki Hotta","doi":"10.1007/BF00360483","DOIUrl":"https://doi.org/10.1007/BF00360483","url":null,"abstract":"<p><p>We have isolated three classes of \"enhancertrap\" lines of Drosophila in which lacZ expression patterns in the imaginal discs are consistent with the idea of a polar (radial and angular) coordinate system of positional information. In the first class (HZ76), a circular pattern was expressed transiently during the mid-third instar larval stage when the radial components of the coordinate are probably generated. The expression patterns of the second class (HZ84) were sector-shaped and circular in the leg disc, suggesting a correlation with both radial and angular coordinate values. The expression patterns found in the third class (PZ63 and PZ22) were circular and appeared to reflect radial positional values. Expression in the latter two classes always began in the presumptive dorsal region of the leg disc and gradually spread to the ventral region. These developmental profiles of expression suggested the existence of a centre that initiates patterned gene expression in the presumptive dorsal region of the leg disc. The PZ22 line showed transient expression during tarsal segmentation, suggesting its involvement in tarsal segment formation. We have cloned the PZ22 gene and partially determined its sequence. The deduced amino acid sequence contained a zinc finger motif found in DNA/RNA binding proteins. By in situ hybridization, we determined that the PZ22 gene was transcribed in the leg disc in a pattern identical to that of the lacZ expression. In addition, it was expressed transiently in the embryonic mesoderm during mesoderm segmentation. The PZ22 gene, therefore, may function both in mesodermal segmentation in the embryo and in tarsal segmentation in the imaginal disc.</p>","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"204 6","pages":"378-391"},"PeriodicalIF":0.0,"publicationDate":"1995-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00360483","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"pag gene-like protein (ABP-25) of the Cynops embryo: regional distribution and gene expression during early embryogenesis.","authors":"Toshiaki Tabata,&nbsp;Kazuhiko Kamio,&nbsp;Tatsuya Tajima,&nbsp;Teruo Kaneda,&nbsp;Akio S Suzuki","doi":"10.1007/BF00360485","DOIUrl":"https://doi.org/10.1007/BF00360485","url":null,"abstract":"<p><p>A maternal protein showing a unique distribution during early Cynops embryogenesis was screened by monoclonal antibody. The antigen protein, designated as ABP-25 (animal blastomere protein, molecular weight 25,000), was distributed uniformly in the uncleaved egg and concentrated into blastomeres of the animal half during cleavage. At the blastula stage, ABP-25 was definitely localized in cells of the animal half and a polarized distribution was observed within the cytoplasm. During gastrulation, immunohistochemical analysis indicated that the reactivity of the marginal zone (presumptive mesoderm) to the monoclonal antibody ABP-25 decreased after involution. At the end of gastrulation, a polarized distribution was still clearly observed in the ventral epidermis, but not in the neuroectoderm. Both Western and Northern blots indicated that the amount of antigen protein and the intensity of gene expresion were almost constant until the neurula stage. The deduced amino acid sequence of the ABP-25 cDNA showed a strong homology (84%) with that of the pag gene associated with cell proliferation.</p>","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"204 6","pages":"400-405"},"PeriodicalIF":0.0,"publicationDate":"1995-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00360485","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of carp-cdx1, a caudal homolog, in embryos of the carp, Cyprinus carpio.","authors":"Henri W J Stroband,&nbsp;Carine Stevens,&nbsp;Geertruy Te Kronnie,&nbsp;Johannis Samallo,&nbsp;Henk Schipper,&nbsp;Bianca Kramer,&nbsp;Lucy P M Timmermans","doi":"10.1007/BF00360482","DOIUrl":"https://doi.org/10.1007/BF00360482","url":null,"abstract":"<p><p>A carp caudal cDNA of 1.3 kb was cloned after screening an early segmentation stage cDNA library with a probe produced by PCR using conserved homeobox sequences as primers and genomic DNA as template. The homeobox gene was called carp-cdxl. The gene appears highly similar to other vertebrate caudal homologs, especially the zebrafish gene cdx(Zf-cad). The possible relationship to homeobox genes within the Hox-C gene complexes is discussed. A weak expression of the gene, detected by in situ hybridization, was found shortly before gastrulation (at 25% epiboly) in cells likely to have a posterior fate. During gastrulation expression became stronger. At the early segmentation stage, cells of the neural keel in the area of the prospective spinal cord expressed the gene. During the progression of segmentation, expression retracted in a caudal direction. The tailbud expressed the gene throughout, but the somites lost expression shortly after their formation. Only the most lateral mesoderm cells maintained expression in the trunk area. Carp-cdxl was also expressed in the endoderm. At 24 h after fertilization the gene was only expressed in the tailbud. At 48 h, no expression could be detected. The expression pattern suggests a function for carp-cdxl in gastrulation and patterning along the anterior-posterior axis of the embryo.</p>","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"204 6","pages":"369-377"},"PeriodicalIF":0.0,"publicationDate":"1995-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00360482","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of genes for two C-type lectins during budding of the ascidian Polyandrocarpa misakiensis.","authors":"Masumi Shimada, Shigeki Fujiwara, Kazuo Kawamura","doi":"10.1007/BF00360486","DOIUrl":"10.1007/BF00360486","url":null,"abstract":"<p><p>Two closely related cDNA fragments, named pTC14-1 and pTC14-2, encoding C-type lectins were cloned from the budding ascidian Polyandrocarpa misakiensis by means of the polymerase chain reaction. The amino acid sequence deduced from pTC14-1 was identical to that of a 14-kDa calcium-dependent galactose-binding lectin, TC-14, that had been purified from this species. Between the two clones, nucleotide sequence similarity was 90%, whilst that of the deduced amino acid sequences was 82%. The cDNA inserts of these clones hybridized weakly with each other. Antisense RNA probes prepared from these clones gave intense hybridization signals on Northern blots of the W strain, but very weak signals on those of the other strains. Therefore, both clones were suggested to originate from the W strain, but from two separate genes, since the base substitution was scattered throughout the entire translated region. The amount of TC14-1 mRNA increased during bud development, and peaked at 36 h after separation of the bud from the parental body wall. At this stage, extracellular matrix containing TC-14 lectin developed in the mesenchymal space around the morphogenetic region of the bud. There was much less TC14-2, than TC14-1 mRNA at every stage of bud development. TC14-1 and TC14-2 mRNAs were detected on Northern blots of RNAs from adults and growing buds, suggesting that these genes can be used as the earliest markers of budding in this species.</p>","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"204 6","pages":"406-411"},"PeriodicalIF":0.0,"publicationDate":"1995-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of the labyrinth in different amphibian species and its correlation with determination of the other ectoderm derivatives.","authors":"Anna Samoilovna Ginsburgt","doi":"10.1007/BF00360480","DOIUrl":"10.1007/BF00360480","url":null,"abstract":"<p><p>The process of labyrinth determination has been studied in three urodelean and seven anuran species by means of homoplastic transplantation of ear region epidermis, defined as the piece of epidermal layer containing the material of the prospective ear vesicle (labyrinth rudiment). The ear region epidermis was grafted onto the abdominal wall of embryos of the same developmental stage. The earliest stage of operation resulting in ectopic ear vesicle formation was determined, suggesting the appearance of organ-specific properties in the ear ectoderm. These properties were enhanced in the further course of development, as indicated by the frequency of ear vesicle formation and by the volume and degree of complexity that the vesicles reached. The data obtained allowed us to arrange the species studied in a sequence, ranging from most Ranidae and Bufo viridis, in which organ-specific properties appear earlier and are most strongly expressed, to Triturus vulgaris in which their expression is least pronounced. Comparison of properties of the material giving rise to the ear vesicle or to several other ectodermal derivatives led to the conclusion that species-specific differences in determination of different ectodermal rudiments are due to species specific properties of the whole ectoderm. These differences appear to be determined by an evolutionary shift of the beginning of gastrulation towards later cleavage cycles.</p>","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"204 6","pages":"351-358"},"PeriodicalIF":0.0,"publicationDate":"1995-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developmental regulation of presence of binding sites for neoglycoproteins and endogenous lectins in various embryonic stages of human lung, liver and heart.","authors":"Klaus Kayser,&nbsp;Sabine André,&nbsp;Gerhard Böhm,&nbsp;Sonia Donaldo-Jacinto,&nbsp;Peter Fritz,&nbsp;Herbert Kaltner,&nbsp;Gian Kayser,&nbsp;Wolf-Peter Kunze,&nbsp;Andreas Nehrlich,&nbsp;Fu-Yue Zeng,&nbsp;Hans-Joachim Gabius","doi":"10.1007/BF02179503","DOIUrl":"https://doi.org/10.1007/BF02179503","url":null,"abstract":"<p><p>Protein-carbohydrate interactions are supposed to play key roles in the mechanisms of cell adhesion, biosignalling and intracellular routing, warranting the analysis of the developmental course of expression of epitopes of this system. Thus, a panel of carrier-immobilized carbohydrate ligands was used as probes, namely lactose,N-acetylgalactosamine,N-acetylglucosamine, mannose, fucose and maltose. Additionally, an antibody to an endogenous β-galactoside-binding lectin (anti-galectin-1), the biotinylated lectin and two further human lectins, namely the macrophage migration inhibitory factor-binding sarcolectin and serum amyloid P component (SAP) that displays selectivity for sulphated sugars and mannose-6-phosphate, were included. They enabled us to assess the extent of the presence of respective binding sites in fixed sections from human lungs (pulmonary epithelial cells), livers (hepatocytes) and hearts (myocard cells) of 10-50 weeks gestation. Invariably, specific binding was detected in the three organ types, at least in certain stages. In most of the cases, the intensity of staining exhibited developmental regulation. The apparent patterns reveal similarities between the different cell types, as seen with immobilizedN-acetylglucosamine as well as with labelled galectin-1 and sarcolectin. However, drastic differences among such patterns with nearly opposite developmental courses do also occur, as detected for carrier-attached mannose and maltose residues. These results point to a potential importance for the detected glycohistochemical features in human development and substantiate the possibility of differential regulation of the presence of binding sites for distinct sugars within a certain organ and between the individual cell types of the monitored organs.</p>","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"204 5","pages":"344-349"},"PeriodicalIF":0.0,"publicationDate":"1995-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02179503","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34823178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Defects in the adult abdominal integument ofDrosophila caused by mutations intorpedo, a DER homolog.","authors":"Kornath Madhavan,&nbsp;Mekkara Mandaravally Madhavan","doi":"10.1007/BF02179501","DOIUrl":"https://doi.org/10.1007/BF02179501","url":null,"abstract":"<p><p>TheDrosophila homolog of the vertebrate EGF receptor (DER) gene is encoded by thetorpedo (top) locus. We examined the role oftop in the development and differentiation of the integument of the adult abdomen ofDrosophila, by analysing these processes in transheterozygotes of twotop alleles. The mutation, when compared to the wild type, affected mitosis, spreading and differentiation of adult epidermal cells derived from the various histoblast and spiracular nests. Our observations indicate that the need for wild-typetop gene product becomes critical after pupation, and the requirement continues throughout the rest of adult development for the normal morphogenesis of the abdominal integument and spiracles.</p>","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"204 5","pages":"330-335"},"PeriodicalIF":0.0,"publicationDate":"1995-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02179501","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eugene Bataillon (1864-1953) and traumatic parthenogenesis.","authors":"Jean-Louis Fischer","doi":"10.1007/BF02179498","DOIUrl":"https://doi.org/10.1007/BF02179498","url":null,"abstract":"","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"204 5","pages":"281-283"},"PeriodicalIF":0.0,"publicationDate":"1995-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02179498","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution, classification, and development ofDrosophila glial cells in the late embryonic and early larval ventral nerve cord.","authors":"Kei Ito,&nbsp;Joachim Urban,&nbsp;Gerhard Martin Technau","doi":"10.1007/BF02179499","DOIUrl":"https://doi.org/10.1007/BF02179499","url":null,"abstract":"<p><p>To facilitate the investigation of glial development inDrosophila, we present a detailed description of theDrosophila glial cells in the ventral nerve cord. A GAL4 enhancer-trap screen for glial-specific expression was performed. Using UAS-lacZ and UAS-kinesin-lacZ as reporter constructs, we describe the distribution and morphology of the identified glial cells in the fully differentiated ventral nerve cord of first-instar larvae just after hatching. The three-dimensional structure of the glial network was reconstructed using a computer. Using the strains with consistent GAL4 expression during late embryogenesis, we traced back the development of the identified cells to provide a glial map at embryonic stage 16. We identify typically 60 (54-64) glial cells per abdominal neuromere both in embryos and early larvae. They are divided into six subtypes under three categories: surface-associated glia (16-18 subperineurial glial cells and 6-8 channel glial cells), cortex-associated glia (6-8 cell body glial cells), and neuropile-associated glia (8-10 nerve root glial cells, 14-16 interface glial cells, and 3-4 midline glial cells). The proposed glial classification system is discussed in comparison with previous insect glial classifications.</p>","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"204 5","pages":"284-307"},"PeriodicalIF":0.0,"publicationDate":"1995-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02179499","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genes controlling posterior gut development in theDrosophila embryo.","authors":"Ruth Harbecke, Judith A Lengyel","doi":"10.1007/BF02179500","DOIUrl":"10.1007/BF02179500","url":null,"abstract":"<p><p>Our present detailed understanding of the genetic mechanisms controlling segmentation has been made possible, in large part, by comprehensive screens of cuticular morphology that identified genes involved in epidermal patterning. To systematically identify genes involved in internal morphogenesis, specifically development of the gut, we have screened mutant embryos produced by a collection of 53 embryonic lethal mutations affecting embryonic pattern formation or differentiation, and a collection of 161 deficiencies covering, in aggregate, approximately 70% of the genome. Staining with the anti-crumbs antibody was used to characterize the Malpighian tubules and hindgut, as well as other internal organs. The geneshuckebein, tailless andwingless, and two previously undescribed loci at 24C/D and 68D/E, are required to establish the primordia for the posterior midgut and hindgut/Malpighian tubules. A locus in region 30A/C is required for extension of the midgut epithelium to surround the yolk, and region 36E/37F is required for outbudding of the Malpighian tubule primordia. Several deficiencies were identified that uncover loci with specific effects on the morphogenesis (elongation, lumen formation) of the hindgut and Malpighian tubules and on the formation of constrictions in the midgut.</p>","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"204 5","pages":"308-329"},"PeriodicalIF":0.0,"publicationDate":"1995-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}