Françoise Huang, Jacques van Helden, Christine Dambly-Chaudière, Alain Ghysen
{"title":"Contribution of the geneextramacrochaetae to the precise positioning of bristles inDrosophila.","authors":"Françoise Huang, Jacques van Helden, Christine Dambly-Chaudière, Alain Ghysen","doi":"10.1007/BF02179502","DOIUrl":"https://doi.org/10.1007/BF02179502","url":null,"abstract":"<p><p>We examine the effect of mutations in theextramacrochaetae (emc) gene on the positioning of macrochaetes on the notum ofDrosophila. We show that, inemc mutants, most of the precursor cells appear earlier than in wild-type individuals, consistent with an antagonizing effect ofemc on the action of the proneural genesachaete andscute. We also show that reducingemc function affects the position of three bristles and/or of their precursors, but has no marked effect on the positioning of the other bristles.</p>","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"204 5","pages":"336-343"},"PeriodicalIF":0.0,"publicationDate":"1995-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02179502","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34823177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Lectin binding sites during Drosophila embryogenesis.","authors":"P Callaerts, V Vulsteke, A De Loof, W Peumans","doi":"10.1007/BF00208490","DOIUrl":"https://doi.org/10.1007/BF00208490","url":null,"abstract":"<p><p>The spectrum of lectin binding sites as it emerges during embryonic development of Drosophila was analysed by means of fluorescein-labelled lectins. As development and morphogenesis proceed, the reaction pattern becomes more and more complex. Mannose/glucose-, mannose-, N-acetylglucosamine- and poly-N-ace-tylglucosamine-specific lectins bind ubiquitously. Nuclear envelopes only have binding sites for wheat germ agglutinin. N-acetylgalactosamine-binding lectins are specific for ectodermal derivatives. Gaβ-3-N-acetylgalac-tosamine-binding lectins are highly selective markers for neural structures, haemocytes and Garland cells. It is also shown that Drosophila laminin is differentially glycosylated. The possible implications of differential and germ layer-specific glycosylation are discussed.</p>","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"204 4","pages":"229-243"},"PeriodicalIF":0.0,"publicationDate":"1995-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00208490","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34823289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Yves Delage (1854-1920) and the ideology behind his research on fécondation.","authors":"Jean-Louis Fischer","doi":"10.1007/BF00208488","DOIUrl":"https://doi.org/10.1007/BF00208488","url":null,"abstract":"","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"204 4","pages":"219-222"},"PeriodicalIF":0.0,"publicationDate":"1995-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00208488","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34823287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Metamorphosin A is a neuropeptide.","authors":"Thomas Leitz, Marion Lay","doi":"10.1007/BF00208495","DOIUrl":"10.1007/BF00208495","url":null,"abstract":"<p><p>A novel biologically active peptide (metamorphosin A, MMA, pEQPGLW.NH<sub>2</sub>) has recently been described. It was isolated from Anthopleura elegantissima and triggers metamorphosis in Hydractinia echinata. Antibodies directed against the C-terminal part of the molecule immunohistochemically stain neurosensory cells and processes in the anterior part of larvae of H. echinata. We assume that in metamorphosis MMA (or a closely related LW-amide) is an internal signal transmitted from the anterior to the posterior body parts. Immunoreactivity is also found in ectodermal nerve processes - but not cell bodies - in the tentacles and in the basal disk of the foot of Hydra magnipapillata. This is, to our knowledge, the first report of LW-amide(s) as (a) neuropeptide(s).</p>","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"204 4","pages":"276-279"},"PeriodicalIF":0.0,"publicationDate":"1995-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Mutations in neuromusculin, a gene encoding a cell adhesion molecule, cause nervous system defects.","authors":"Artur Kania, Hugo J Bellen","doi":"10.1007/BF00208493","DOIUrl":"https://doi.org/10.1007/BF00208493","url":null,"abstract":"<p><p>The Drosophila neuromusculin (nrm) gene encodes an immunoglobulin-like (Ig-like) cell adhesion molecule expressed in the precursors of the embryonic peripheral nervous system (PNS), in the midline precursors of the central nervous system (CNS), and in muscles. During the initial phases of CNS axonogenesis, nrm is expressed in cells involved in the development of commissures and longitudinal tracts. Mutations which alter expression of nrm mRNAs cause aberrant development of commissures and longitudinal axon pathways. Defects in the PNS and muscles of nrm mutants are also observed. In most nrm embryos, abnormal development can be detected in a subset of abdominal segments; however, in approximately 1 of 10 nrm embryos, the defects extend to all segments. Herein, we present evidence that nrm plays an important role in early morphogenesis, possibly by mediating or facilitating inductive cell contacts and movements.</p>","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"204 4","pages":"259-270"},"PeriodicalIF":0.0,"publicationDate":"1995-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00208493","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Astrid Zimmermann, Anke Haina, Ute Gröschel-Stewart
{"title":"Neural and smooth muscle development in the chicken gizzard.","authors":"Astrid Zimmermann, Anke Haina, Ute Gröschel-Stewart","doi":"10.1007/BF00208494","DOIUrl":"https://doi.org/10.1007/BF00208494","url":null,"abstract":"<p><p>The development of the autonomic ganglia of Auerbach's plexus and gizzard smooth muscle was studied in chicken embryos. Nervous system and smooth-muscle-specific antibodies were employed in immunofluorescence stainings on tissue sections to investigate the temporal and spatial frame of neural and muscular differentiation in relation to each other. Subserosal clusters of neural cells were clearly demonstrable at embryonic day 5 (ED5), the earliest stage analysed, with the monoclonal antibody El (SGIII-1). Fine nerve fibres (ED6) and, later, large axon bundles projecting from subserosal neuron clusters towards the lumen were followed and found to reach the luminal border by ED11. Already in early development the area of the future laminar tendons on the ventral and dorsal surface of the gizzard was devoid of neuroblasts, and nerve fibres were not extending to the muscle-tendon borderline until ED16. Double stainings with antibodies to smooth muscle myosin (SMM) and El revealed that SMM expression, taken as an indicator for muscle differentiation, followed neural growth. It was first detectable in close apposition to the differentiating neuroblasts in the caudal and cranial portion of the gizzard at ED6. With further development, myosin expression proceeded inward towards the lumen in a wave which followed the ingrowth of E1-positive nerve fibres from the prospective Auerbach plexus. Neuromuscular differentiation deviated from this pattern in the lateral tendon area where nerve growth was delayed and myosin expression preceeded the arrival of E1-positive nerve fibres. The findings suggest that the gizzard could serve as a model system for the analysis of potential early nervous system imprints on smooth premuscle mesenchyme differentiation.</p>","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"204 4","pages":"271-275"},"PeriodicalIF":0.0,"publicationDate":"1995-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00208494","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The chicken retinoid-X-receptor-α (RXR-α) gene and its expression in the developing limb.","authors":"E A P Seleiro, A Rowe, P M Brickell","doi":"10.1007/BF00208491","DOIUrl":"https://doi.org/10.1007/BF00208491","url":null,"abstract":"<p><p>Antero-posterior (a-p) patterning of the vertebrate limb bud is controlled by signals from the polarizing region, a group of cells in the posterior mesenchyme of the bud. Application of retinoic acid to the anterior margin of the chick wing bud induces polarizing region activity in anterior mesenchyme cells, resulting in digit duplications. Retinoic acid acts by binding to nuclear retinoid receptors, and so regulating expression of target genes. Retinoid receptors of the RXR class are essential for this activity. We have previously described a chicken RXR-γ cDNA clone (Rowe et al. 1991a). In this paper we report the isolation and characterization of a chicken RXR-α cDNA clone, and show by northern blotting that an RXR-α mRNA of approximately 5 kilobases is present in a range of tissues in embryonic and adult chickens. In situ hybridization experiments showed that RXR-α transcripts were present throughout the epithelium and mesenchyme of the chick wing bud at stages when retinoic acid can affect a-p patterning. In contrast, RXR-γ transcripts were undetectable in these cells, being restricted to peripheral nervous tissue in the bud. These data suggest that RXR-α, but not RXR-γ, could mediate the effects of locally applied retinoic acid on a-p patterning in the chick wing bud.</p>","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"204 4","pages":"244-249"},"PeriodicalIF":0.0,"publicationDate":"1995-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00208491","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Xenopus laevis tadpole limb regeneration in vivo and in vitro: thyroxine directly promotes blastemal cell proliferation and morphogenesis.","authors":"G La Mesa, S Bernardini, S M Cannata, S Filoni","doi":"10.1007/BF00208489","DOIUrl":"https://doi.org/10.1007/BF00208489","url":null,"abstract":"<p><p>Regeneration in hindlimbs of Xenopus laevis larvae which were amputated at stage 53 and 55 through the tarsalia region is promoted by thyroxine (T4), while propyl-thiouracil (PTU) inhibits regeneration when compared to controls. In this paper, by in vivo and in vitro experiments, we demonstrate that the promoting effect of T4 on the regenerative processes of larval X. laevis hindlimbs is a direct effect of this hormone on the blastemal cells. By contrast, the inhibitory effect of PTU on the regenerative process is not due to a direct effect on blastemal cells or to a general toxic effect on the treated larvae, but is related to hypothyroidism induced by the drug. We find that: (i) an increase in blastemal cell proliferation is observed not only in blastemata of T4-treated larvae, but also in blastemata cultured in vitro in a medium supplemented with T4; (ii) the renegerative process is accelerated not only in larvae reared in T4 but also in larvae submitted to a combined treatment of T4 and PTU; (iii) inhibition of cell proliferation is observed in blastemata of PTU-reared larvae but not in blastemata cultured in vitro in a medium supplemented with PTU. Experiments on thyroidless larvae (which were submitted to transplantation of hindlimbs from larvae at stages 53 and 55 followed by amputation of their own right hindlimb and the transplanted limbs) have shown that without thyroid hormone the regenerative process is arrested at cone stage and the promoting effect of T4 treatment is dependent on limb stage and amputation level.</p>","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"204 4","pages":"223-228"},"PeriodicalIF":0.0,"publicationDate":"1995-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00208489","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34823288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Mutations in supernova, indicate that this gene is required for the division of germ line cells in Drosophila.","authors":"L Billett, N G Brink","doi":"10.1007/BF00208492","DOIUrl":"https://doi.org/10.1007/BF00208492","url":null,"abstract":"<p><p>Mutations in supernova, previously shown to uncouple chromosome replication from segregation during cleavage in Drosophila embryos, also sanctions extra divisions of cystoblasts and spermatoblasts. This leads either to the formation of egg chambers which contain more than fifteen nurse cells or testes which have an excess of spermatocytes. In maturing egg chambers two potential oocytes may be specified in which case they are often ectopically located and connected with surrounding nurse cells by four ring canals. However, a typical oocyte nucleus is not always present and these chambers usually become necrotic and degenerate. The nurse cells are of variable size, but are still interconnected by a system of ring canals. They all possess a polyploid nucleus. Sequestering of maternal mRNA's from the nurse cells into the potential oocyte(s) takes place but there is no localization of this maternal information within the oocyte probably because of defective microtubule assembly. Many spermatocytes fail to complete meiosis so that bundles of spermatids are reduced in size and the males have reduced fertility. It is proposed that this gene is indirectly involved in regulating the timing of mitotic divisions in both cystoblasts and spermatoblasts through its interference with microtubule assembly which is consistent with its role during embryogenesis.</p>","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"204 4","pages":"250-258"},"PeriodicalIF":0.0,"publicationDate":"1995-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00208492","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Michaela Scheucher, Petra Dege, Jutta Lef, Sigurd Hille, Walter Knöchel
{"title":"Transcription patterns of four different fork head/HNF-3 related genes (XFD-4, 6, 9 and 10) in Xenopus laevis embryos.","authors":"Michaela Scheucher, Petra Dege, Jutta Lef, Sigurd Hille, Walter Knöchel","doi":"10.1007/BF00241274","DOIUrl":"https://doi.org/10.1007/BF00241274","url":null,"abstract":"<p><p>We have isolated and sequenced four different members of the Xenopus fork head domain related multigene family (XFD-4, 6, 9 and 10). These genes show a high degree of sequence conservation inside the evolutionary conserved fork head domain but they completely diverge outside this region. All four XFD genes are transcribed during embryogenesis but they are activated at different developmental stages. To investigate the localisation of transcripts we performed whole mount in situ hybridizations. We detected XFD-4 transcripts in somitogenic mesoderm, pronephros, pronephric duct, heart and the tip of the tail. XFD-6 transcripts were confined to migrating neural crest cells originating from the mesencephalon and rhombencephalon. Transcriptional activity of XFD-9 was observed in the region of rotating somites and the posterior part of the pronephric duct. XFD-10 transcripts were detected in lateral cell stripes located at the hyoid and the anterior branchial arches.</p>","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"204 3","pages":"203-211"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00241274","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34823068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}