Roux's archives of developmental biology : the official organ of the EDBO最新文献_第10页

Localization and quantification of cyclin A and B mRNA during the embryonic development of Patella vulgata. 外翻髌骨胚胎发育过程中细胞周期蛋白A和B mRNA的定位和定量。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1995-01-01 DOI: 10.1007/BF00241267

A van der Kooij, H J Goedemans, A E van Loon

{"title":"Localization and quantification of cyclin A and B mRNA during the embryonic development of Patella vulgata.","authors":"A van der Kooij, H J Goedemans, A E van Loon","doi":"10.1007/BF00241267","DOIUrl":"https://doi.org/10.1007/BF00241267","url":null,"abstract":"As the first five cleavages of the Patella vulgata embryo are synchronous, they are well suited to determine the mRNA level of cyclin A and B genes in an embryo. During the third and fourth cleavage cycle the quantity of A and B mRNA is regulated in a cell-cycle-dependent way, reaching a high level between cleavages and a lower level just after mitosis. This implies that transcription of the cyclin genes occurs before the overall transcription increases directly after the fifth cleavage. During the first cleavages cyclin A and B mRNA is localized in distinct parts of the cytoplasm. Between two successive cell devisions it is found as a crescent-shaped domain at the peripheral side of the nucleus. At cytokinesis it is present between two separating nuclei and at newly formed cell membranes. At the fifth cleavage this localization disappears. Changes in the expression pattern of cyclin A and B may be expected after the fifth cleavage, when the first cells become arrested in cell division and differentiate. The mechanism causing cell division arrest of these primary trochoblasts is still unknown. Cell division arrest caused by the absence of cyclin A and/or B mRNA could be conditional for further differentiation. However, a decrease in cyclin A and B mRNA level in the trochoblasts is not detectable until 4 h after their last division. Later in development no cyclin A and B mRNA can be detected in these cells, whereas cyclin A and B mRNA is present in other cells of the embryo. Thus, the absence of cyclin A and B mRNA in primary trochoblasts, and in the later differentiating secondary and accessory trochoblasts is not obligatory for cell division arrest or cell differentiation.","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"204 3","pages":"157-163"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00241267","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34823162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fission yeast tmsl protein abrogates normal development in Xenopus laevis embryos. 分裂酵母tms1蛋白破坏非洲爪蟾胚胎的正常发育。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1995-01-01 DOI: 10.1007/BF00241273

Peter Wagner, Michael Hoever, Katrin Appel, Walter Knöchel, Mathias Montenarh

引用次数: 0

Muscle determinants in the ascidian egg are inactivated by UV irradiation and the inactivation is partially rescued by injection of maternal mRNAs. 海鞘卵中的肌肉决定因子在紫外线照射下失活，部分失活可通过母体mrna的注射来恢复。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1995-01-01 DOI: 10.1007/BF00241270

Y Marikawa, S Yoshida, N Satoh

{"title":"Muscle determinants in the ascidian egg are inactivated by UV irradiation and the inactivation is partially rescued by injection of maternal mRNAs.","authors":"Y Marikawa, S Yoshida, N Satoh","doi":"10.1007/BF00241270","DOIUrl":"10.1007/BF00241270","url":null,"abstract":"The ascidian egg contains cytoplasmic determinants that specify the fate of larval muscle cells. In a previous study, we developed an experimental system to identify the molecular nature of muscle determinants, in which unfertilized Ciona savignyi eggs were fragmented into four pieces by centrifugation. When inseminated, only nucleated fragments (red fragments) develop into partial embryos that only show differentiation of epidermal cells. One type of enucleated fragment (black fragment) has the remarkable ability to promote muscle differentiation when fused with red fragments. In the present study, using this experimental system, we investigated the molecular nature of muscle determinants. UV irradiation of black fragments suppressed the ability to promote expression of the muscle-specific protein, myosin heavy chain. The wavelength of UV light responsible for the inactivation (250-275 nm) suggested that UV-sensitive targets are nucleic acids. Injection of poly(A)+ RNA isolated from an un-irradiated black-fragment-rich fraction into UV-irradiated black fragments partially recovered the ability to promote the expression of myosin heavy chain protein. Poly(A)+ RNA from a red-fragment-rich fraction did not rescue the suppression of UV-irradiated black fragments. These results suggest that maternal mRNAs enriched in black fragments are closely associated with muscle determinants in the ascidian egg.","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"204 3","pages":"180-186"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34823165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temporally and spatially restricted expression of a gland cell gene during regeneration and in vitro transdifferentiation in the hydrozoan Podocoryne carnea. 水螅体Podocoryne carnea再生和体外转分化过程中腺体细胞基因的时空限制表达。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1995-01-01 DOI: 10.1007/BF00241268

C D Baader, P Schuchert, V Schmid, Reinhard Heiermann, Günter Plickert

{"title":"Temporally and spatially restricted expression of a gland cell gene during regeneration and in vitro transdifferentiation in the hydrozoan Podocoryne carnea.","authors":"C D Baader, P Schuchert, V Schmid, Reinhard Heiermann, Günter Plickert","doi":"10.1007/BF00241268","DOIUrl":"10.1007/BF00241268","url":null,"abstract":"An antiserum to transdifferentiated striated muscle cells from the medusa of Podocoryne carnea was prepared and used to screen a λ gt11-expression library prepared from gonozoids of P. carnea. We isolated a cDNA clone termed Pod-EPPT with at least 63 tandem repeats of the tetrapeptide-motive glu-pro-pro-thr, named Pod-EPPT. Using Pod-EPPT as a molecular marker for head quality the morphological relationship between the two metagenic life stages of this hydroid, the polyp and the medusa, was studied. In situ hybridization demonstrated that expression of the gene corresponding is restricted to secretory cells in the endoderm of the oral hypostome region of polyps and medusae and, presumably, to progenitor cells of this type. Cells expressing Pod-EPPT could not be observed in the larval stage. During head regeneration in polyps, Pod-EPPT expression is upregulated soon after head removal in previously non-expressing cells and in newly differentiating secretory cells. This activation of a head-specific gene precedes the morphologically obvious events of head regeneration. Pod-EPPT is one of the genes that are activated during manubrium (mouth) regeneration from experimentally combined subumbrellar plate endoderm and striated muscle of the medusa.","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"204 3","pages":"164-171"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34823163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heart targeting of retroviral expression in avian embryos: a species-independent phenomenon. 逆转录病毒在禽类胚胎中的心脏靶向表达:一种非物种现象。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1995-01-01 DOI: 10.1007/BF00241275

I Hlozanek, D Luton, F Dieterlen-Lièvre, T Jaffredo

{"title":"Heart targeting of retroviral expression in avian embryos: a species-independent phenomenon.","authors":"I Hlozanek, D Luton, F Dieterlen-Lièvre, T Jaffredo","doi":"10.1007/BF00241275","DOIUrl":"https://doi.org/10.1007/BF00241275","url":null,"abstract":"A replication-incompetent retroviral vector derived from spleen necrosis virus (SNV), in which the viral structural genes gag, pol, and env were replaced with the bacterial β-galactosidase gene lacZ, was used to infect embryos from outbred and inbred chicken lines, japanese quail and duck between embryonic day 0 and 13. LacZ expression was restricted to a few organs or cell types, and this distribution was not influenced by the different routes of inoculation tested but was specified by the age of the embryo at the time of inoculation. Inoculations at E0-E1 beneath or onto the blastodisc resulted in lacZ expression in ectodermal derivatives, i.e. skin and neural structures. From E2 onwards, heart muscle and skin were the preferential targets in all the species or inbred lines tested. Heart muscle was positive in 100% of the embryos displaying lacZ+ clones. Skin exhibited on and off periods depending on the age at inoculation. No lacZ-positive clones were detected in chick embryos infected after Ell. Outbred chick embryos displayed the largest array of organs labelled (heart, skin, liver, gizzard) while quail and duck embryos exhibited a more restrictive pattern. These results are of import if the vector is to be used as a tool to map lineages or to transfer genes into the developing embryo.","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"204 3","pages":"212-218"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00241275","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34823069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Different spatial distribution of mRNAs for activin receptors (type IIA and IIB) and follistatin in developing embryos of Xenopus laevis. 非洲爪蟾胚胎中激活素受体（IIA型和IIB型）和卵泡抑素mrna的空间分布差异

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1995-01-01 DOI: 10.1007/BF00241269

C Koga, K Tashiro, K Shiokawa

{"title":"Different spatial distribution of mRNAs for activin receptors (type IIA and IIB) and follistatin in developing embryos of Xenopus laevis.","authors":"C Koga, K Tashiro, K Shiokawa","doi":"10.1007/BF00241269","DOIUrl":"10.1007/BF00241269","url":null,"abstract":"Spatial distribution of mRNAs for activin receptors and follistatin was studied by Northern blot hybridization using RNAs from different parts of dissected Xenopus embryos. mRNAs of two activin receptors (type IIA and IIB) occurred uniformly in pre-gastrular embryos, but occurred in larger amounts in ectoderm (in gastrulae), neural plate (in neurulae) and anterior (head) regions (in tailbud embryos) than in other embryonic regions. By contrast, follistatin mRNA appeared almost exclusively in the dorsal mesoderm including invaginating organizer region at the gastrula stage, in notochord and in dorsal ectoderm at the neurula stage, then in anterior part at the tailbud stage. The localized patterns of the distribution of these mRNAs may be due to the regionally different zygotic expression of genes in embryos at later stages. From the relatively widespread pattern of distribution of their mRNAs, we assume that both type IIA and type IIB activin receptors have broad functions in ectodermal and neural differentiation. On the other hand, follistatin mRNA showed quite a restricted pattern of expression, and therefore, we assume that follistatin may have functions more specifically related to the sites of expression of its mRNA. Thus, follistatin may be involved in the differentiation of notochord itself and/or directly be responsible for organizer functions such as neural induction and subsequent differentiation of induced neural tissues at the gastrula and later stages.","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"204 3","pages":"172-179"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34823164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeted mutagenesis and genetic analysis of a Drosophila receptor-linked protein tyrosine phosphatase gene. 果蝇受体连接蛋白酪氨酸磷酸酶基因的靶向诱变和遗传分析。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1995-01-01 DOI: 10.1007/BF00241271

B A Hamilton, A Ho, Kai Zinn

{"title":"Targeted mutagenesis and genetic analysis of a Drosophila receptor-linked protein tyrosine phosphatase gene.","authors":"B A Hamilton, A Ho, Kai Zinn","doi":"10.1007/BF00241271","DOIUrl":"https://doi.org/10.1007/BF00241271","url":null,"abstract":"Several Drosophila receptor-linked protein tyrosine phosphatases (R-PTPs) are selectively expressed on axons of the developing embryonic central nervous system. The extracellular domains of these axonal R-PTPs are homologous to neural adhesion molecules. Thus, R-PTPs may directly couple cell recognition to signal transduction via control of tyrosine phosphorylation. To examine the function of these molecules during nervous system development, we wished to generate mutations in R-PTP genes. It was unclear whether a mutation in a single R-PTP gene would confer lethality, however, because the similarities in sequence and expression pattern between the axonal R-PTPs suggest that they may have partially redundant functions. To circumvent this problem, we developed a directed mutagenesis strategy based on local transposition of P elements, and used this approach to isolate a null mutation in the DPTP99A gene. This strategy, which we describe in detail here, should be applicable to any Drosophila gene within a lettered division of an appropriately marked P element. Flies lacking DPTP99A expression are viable and fertile, and we have been unable to detect any alterations in the embryonic nervous system of DPTP99A embryos using a variety of antibody markers.","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"204 3","pages":"187-192"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00241271","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34823166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Onset of transcription in Patella vulgata coincides with cell cycle elongation and expression of tubulin genes. 外翻髌骨的转录开始与细胞周期延长和微管蛋白基因的表达一致。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1995-01-01 DOI: 10.1007/BF00241272

André E van Loon, Hans J Goedemans, Mo E M Weijtens, A J J M Daemen

{"title":"Onset of transcription in Patella vulgata coincides with cell cycle elongation and expression of tubulin genes.","authors":"André E van Loon, Hans J Goedemans, Mo E M Weijtens, A J J M Daemen","doi":"10.1007/BF00241272","DOIUrl":"10.1007/BF00241272","url":null,"abstract":"In this study we show that the onset of embryonic transcription in the marine snail Patella vulgata coincides with the start of the sixth cleavage, when the cell-cycle elongates and divisions become asynchronous. Changes in mRNA content before and after onset of transcription were initially demonstrated by in vitro translation of isolated mRNA from different stages. Before the sixth cleavage, three major mRNAs encoding proteins of 36, 50 and 52 kDa were present. These proteins probably correspond to cyclin A and B and ribonucleotide reductase. After this stage, three major proteins with molecular weights of 36.5, 52.5 and 53 kDa were found after in vitro translation. Via hybrid selected translation and differential screening cDNAs corresponding to the 52.5 and 53 kDa proteins were cloned. The encoded proteins resemble tubulins from other animals to a high extent (between 96.5 and 93.1% identity for α-tubulin and 97.9 and 75.9% for β-tubulin). The 36.5 kDa protein is the previously described actin. Both tubulins were expressed at or shortly after the first asynchronous division after the fifth cleavage.","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"204 3","pages":"193-197"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34823167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Drosophila glial development is regulated by genes involved in the control of neuronal cell fate. 果蝇的神经胶质发育受参与控制神经元细胞命运的基因调控。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1994-12-01 DOI: 10.1007/BF00361106

Heidi B Nelson, Allen Laughon

引用次数: 2

Cell-specific and spatio-temporal expression of intestinal fatty acid-binding protein gene during amphibian metamorphosis. 两栖动物变态过程中肠道脂肪酸结合蛋白基因的细胞特异性和时空表达。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1994-12-01 DOI: 10.1007/BF00361110

Atsuko Ishizuya-Oka, Atsumi Shimozawa, Hiroyuki Takeda, Yun-Bo Shi

{"title":"Cell-specific and spatio-temporal expression of intestinal fatty acid-binding protein gene during amphibian metamorphosis.","authors":"Atsuko Ishizuya-Oka, Atsumi Shimozawa, Hiroyuki Takeda, Yun-Bo Shi","doi":"10.1007/BF00361110","DOIUrl":"10.1007/BF00361110","url":null,"abstract":"Intestinal fatty acid-binding protein (IFABP) gene is known to be regulated during Xenopus metamorphosis. To determine the relationship between its regulation and cellular differentiation during metamorphosis, we have examined the distribution of IFABP mRNA in the Xenopus digestive tract by in situ hybridization techniques. Throughout all stages examined, transcripts of IFABP gene were observed exclusively in absorptive epithelial cells of the small intestine, and they decreased in amount towards the posterior intestine. Around stage 58, just before metamorphic climax, IFABP mRNA level began to decrease in larval absorptive cells that still remained intact morphologically. Thereafter, IFABP mRNA was no longer detected among larval cells. In turn, at stage 62, IFABP mRNA became detectable in some of the newly formed adult epithelium that had not yet developed a brush border, but not in the remaining larval cells. By the end of metamorphosis, IFABP mRNA became more abundant towards the crest of intestinal folds. These results suggest that IFABP gene expression is specific for absorptive epithelial cells of the small intestine and is regionally regulated along the intestinal anterior-posterior axis in both tadpoles and frogs and also along the trough-crest axis of frog intestinal folds. In addition, our present study directly shows that IFABP mRNA level decreases in larval absorptive cells but increases in adult ones during metamorphosis, preceding morphological changes of both types of cells. Therefore, the regulation of IFABP gene is an early event during both larval epithelial cell death and adult epithelial cell differentiation.","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"204 2","pages":"150-155"},"PeriodicalIF":0.0,"publicationDate":"1994-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0