Roux's archives of developmental biology : the official organ of the EDBO最新文献_第7页

Tissue specific loss of proliferative capacity of parthenogenetic cells in fetal mouse chimeras. 胚胎小鼠嵌合体孤雌生殖细胞增殖能力的组织特异性丧失。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1995-08-01 DOI: 10.1007/BF00360851

R Bender, R Fundele, M A Surani, L-L Li, R Kothary, D O Fürst, B Christ

{"title":"Tissue specific loss of proliferative capacity of parthenogenetic cells in fetal mouse chimeras.","authors":"R Bender, R Fundele, M A Surani, L-L Li, R Kothary, D O Fürst, B Christ","doi":"10.1007/BF00360851","DOIUrl":"https://doi.org/10.1007/BF00360851","url":null,"abstract":"Parthenogenetic cells are lost from fetal chimeras. This may be due to decreased proliferative potential. To address this question, we have made use of combined cell lineage and cell proliferation analysis. Thus, the incorporation of bromodeoxyuridine in S-phase was determined for both parthenogenetic and normal cells in several tissues of fetal day 13 and 17 chimeras. A pronounced reduction of bromodesoxyuridine incorporation by parthenogenetic cells at both developmental stages was only observed in cartilage. In brain, skeletal muscle, heart and intestinal epithelium, this reduction was either less pronounced or observed only at one of the developmental stages analysed. No difference between parthenogenetic and normal cells was observed in epidermis and ganglia. Our results show that a loss of proliferative potential of parthenogenetic cells during fetal development contributes to their rapid elimination in some tissues. The analysis of the fate of parthenogenetic cells in skeletal muscle and cartilage development demonstrated different selection mechanisms in these tissues. In skeletal muscle, parthenogenetic cells were largely excluded from the myogenic lineage proper by early post-midgestation. In primary hyaline cartilage, parthenogenetic cells persisted into adulthood but were lost from cartilages that undergo ossification during late fetal development.","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"204 7-8","pages":"436-443"},"PeriodicalIF":0.0,"publicationDate":"1995-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00360851","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Cell adhesion to extracellular matrix is different in marine hydrozoans compared with vertebrates. 与脊椎动物相比，海洋水生动物细胞对细胞外基质的粘附性不同。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1995-08-01 DOI: 10.1007/BF00360854

V Schmid, B Aeschbach, K Agata, J Kosaka, S Reber-Müller, N Sprenger, G Eguchi

{"title":"Cell adhesion to extracellular matrix is different in marine hydrozoans compared with vertebrates.","authors":"V Schmid, B Aeschbach, K Agata, J Kosaka, S Reber-Müller, N Sprenger, G Eguchi","doi":"10.1007/BF00360854","DOIUrl":"10.1007/BF00360854","url":null,"abstract":"Extracellular matrices (ECMs) of phylogenetically very distant organisms were tested for their ability to support cell adhesion, spreading and DNA replication in reciprocal xenograft adhesion tests. Mechanically dissociated cells of the medusa Podocoryne carnea (Cnidaria, Hydrozoa) were seeded on ECMs of polyps and medusa, and on several ECM glycoproteins or entire ECMs from vertebrates. In reciprocal experiments, cells from different vertebrate cell-lines were seeded on ECMs of polyps, medusae and also on electrophoresed and blotted extracts of both types of ECMs. The results demonstrate that medusa cells adhere and spread on polyp and medusa ECMs but do not recognize vertebrate ECMs or purified ECM glycoproteins. Vertebrate cells in contrast adhere, spread and proliferate on ECMs of polyps and medusae. The number of attached cells depends on the cell type, the type of ECM and, in certain cases, on the stage of the cell cycle. Cell adhesion experiments with pretreated ECMs of polyps and medusae, e.g. oxidation of carbohydrate residues with sodium-metaperiodate, or blocking of certain carbohydrate moieties with the lectin wheat germ agglutinin or a carbohydrate-specific monoclonal antibody, demonstrate that ECM carbohydrates are more important for cell-ECM interactions of medusa cells than for vertebrate cells. Furthermore, the experiments indicate that polyp and medusa ECMs contain different components which strongly modulate adhesion, spreading and DNA replication of vertebrate cells.","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"204 7-8","pages":"465-476"},"PeriodicalIF":0.0,"publicationDate":"1995-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proliferation and differentiation of androgenetic cells in fetal mouse chimeras. 胚胎小鼠嵌合体中雄激素细胞的增殖和分化。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1995-08-01 DOI: 10.1007/BF00360857

R Fundele, A Herzfeld, L-L Li, S C Barton, M A Surani

{"title":"Proliferation and differentiation of androgenetic cells in fetal mouse chimeras.","authors":"R Fundele, A Herzfeld, L-L Li, S C Barton, M A Surani","doi":"10.1007/BF00360857","DOIUrl":"https://doi.org/10.1007/BF00360857","url":null,"abstract":"The properties of androgenetic cells and their ability to proliferate and differentiate were examined in post-midgestation chimeras. In several tissues, namely the brain, cardiac muscle, skeletal muscle and intestinal epithelium, the rate of proliferation of androgenetic cells was higher than that of normal cells in day 13 embryos. This higher rate of proliferation was however less pronounced by day 17-18 of development. It is possible that IGF2, a major growth factor regulating fetal growth, could play a role in the increased proliferation of androgenetic cells. Igf2 is also an imprinted gene that is expressed only when inherited paternally. Indeed, in the smooth muscle, cartilage and intestinal epithelium, patches of androgenetic (ag) cells exhibited higher levels of IGF2 mRNA than neighbouring wild-type cells. Surprisingly, we also detected expression of Igf2 in ag cells of ectodermal origin; this gene is not normally expressed in this lineage. This expression was observed in the brain, epidermis and in the epithelium of the tongue. We attempted to confirm the identity and differentiation status of ag cells with the help of cell-type specific antibodies and lectins. Evidence for only one of the cell types analysed, i.e. the goblet cells of the gut, suggests a delay or aberrant differentiation of ag cells.","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"204 7-8","pages":"494-501"},"PeriodicalIF":0.0,"publicationDate":"1995-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00360857","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Expression domains of the Cf1a POU domain protein during Drosophila development. 果蝇发育过程中Cf1a POU结构域蛋白的表达域。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1995-08-01 DOI: 10.1007/BF00360858

A N Billin, S J Poole

引用次数: 1

Segmental differentiation processes in embryonic muscle development of the grasshopper. 蚱蜢胚胎肌肉发育的节段分化过程。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1995-08-01 DOI: 10.1007/BF00360853

G Steffens, W Kutsch, F Xie, H Reichert

{"title":"Segmental differentiation processes in embryonic muscle development of the grasshopper.","authors":"G Steffens, W Kutsch, F Xie, H Reichert","doi":"10.1007/BF00360853","DOIUrl":"https://doi.org/10.1007/BF00360853","url":null,"abstract":"To analyse segmental differentiation processes in muscle development, we studied the embryogenesis of the ventral body wall muscles in thoracic and abdominal segments of the grasshopper Schistocerca gregaria at the identified cell level. We visualized differentiating muscle pioneer and muscle precursor cells by staining with a muscle-specific monoclonal antibody and with rhodamine-coupled phalloidin. Our results show that a similar pattern of serially reiterated early muscle pioneers is initially established in all segments. Subsequently, two major segmental differentiation processes occur. First, segment-specific sets of additional, later differentiating muscle pioneers are generated de novo. Second, segment-specific sets of existing early muscle precursors are eliminated through atrophy and eventual loss. These events have consequences for matching homonomy of muscles and their innervating motoneurons. Taken together, these processes in the embryo, in concert with postembryonic differentiation events, play critical roles in shaping the highly specialized muscular structures of the mature animal.","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"204 7-8","pages":"453-464"},"PeriodicalIF":0.0,"publicationDate":"1995-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00360853","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

The relationship between migration and chondrogenic potential of trunk neural crest cells in Ambystoma mexicanum. 墨西哥Ambystoma mexicanum主干神经嵴细胞迁移与成软骨潜能的关系。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1995-08-01 DOI: 10.1007/BF00360855

A C Graveson, B K Hall

引用次数: 5

Distribution of androgenetic cells in fetal mouse chimeras. 雄激素细胞在胚胎小鼠嵌合体中的分布。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1995-08-01 DOI: 10.1007/BF00360856

R Fundele, R Krause, S C Barton, M A Surani, B Christ

引用次数: 3

Developmental asynchrony caused by steep temperature gradients does not impair pattern formation in the wasp, Pimpla turionellae L. 陡峭的温度梯度引起的发育不同步不影响黄蜂的模式形成，Pimpla turionellae L。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1995-08-01 DOI: 10.1007/BF00360852

J Niemuth, R Wolf

{"title":"Developmental asynchrony caused by steep temperature gradients does not impair pattern formation in the wasp, Pimpla turionellae L.","authors":"J Niemuth, R Wolf","doi":"10.1007/BF00360852","DOIUrl":"10.1007/BF00360852","url":null,"abstract":"Generating developmental gradients by temperature gradients established within a developing organism is an easy, non-invasive technique to study physiological interdependencies between locally separated subsystems. A linear temperature gradient of about 10° C/mm was maintained up to 5 h in either direction along the long axis of a long-germ-type hymenopteran egg, which was simultaneously filmed by the 16 mm timelapse technique. The result was a dramatic desynchronization of development, which between the egg poles could reach up to 9.3 h relative to normal development. Within the same egg, up to seven mitotic waves (i.e. eight different nuclear generations) were observed at the same time, and the subsequent cellularization process was extremely asynchronous. The \"initial regions\" of the mitotic waves, the \"fountain flow\" of the ooplasm, and the gastrulation process were shifted towards the egg pole kept at higher temperatures. Developmental processes occurring successively in normal development now took place simultaneously, with either direction of the temperature gradient. For instance, while gastrulation had started in the warm region, midblastula transition and cellularization were in progress in the middle of the egg, and intravitelline nuclear multiplication occurred at the cold pole, by rapid and still biphasic cell cycles. In some respects, development resembled that of a short-germ-type insect egg. Nevertheless, the developmental processes were resynchronized after the temperature gradient was switched off. Surprisingly, the extreme desynchronization during early development did not affect the segment pattern of the resulting embryos. The technique of inducing well-defined developmental asynchronies might be applied in Drosophila to analyse the subtle interplay between maternal and zygotic gene activities described in this species.","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"204 7-8","pages":"444-452"},"PeriodicalIF":0.0,"publicationDate":"1995-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fate mapping study of the splanchnopleural mesoderm of the 1.5-day-old chick embryo. 1.5日龄鸡胚内脏胸膜中胚层命运定位研究。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1995-06-01 DOI: 10.1007/BF00360484

Susumu Matsushita, S Matsushita

引用次数: 0

The effects of the glucocorticoid, dexamethasone, on the development of the Drosophila embryo. 糖皮质激素地塞米松对果蝇胚胎发育的影响。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1995-06-01 DOI: 10.1007/BF00360481

Teresa Ruth Strecker, Peng Li, Sean Austin McGhee, Debby Ham, Su Kyong Smith, Jennifer Ann Schreck, Sue Jung Youn, Philantha Sue-Hwa Kon

{"title":"The effects of the glucocorticoid, dexamethasone, on the development of the Drosophila embryo.","authors":"Teresa Ruth Strecker, Peng Li, Sean Austin McGhee, Debby Ham, Su Kyong Smith, Jennifer Ann Schreck, Sue Jung Youn, Philantha Sue-Hwa Kon","doi":"10.1007/BF00360481","DOIUrl":"https://doi.org/10.1007/BF00360481","url":null,"abstract":"We have investigated the effects of the glucocorticoid, dexamethasone, and five structural analogs on Drosophila development in an effort to identify steroid ligands that may play a role in the embryogenesis of this organism. Embryos were exposed to glucocorticoids either by direct culture in supplemented medium, or by examining embryos from adult flies raised on supplemented fly food. After exposure, embryos were examined for developmental defects. At a morphological level, exposure to dexamethasone disrupts the dorsolateral folding of the amnioserosa during germ band extension. In addition, germ band retraction and dorsal closure is also disrupted. The phenocritical period of these effects is within the first 4 h of embryogenesis. This response is dosage sensitive, with embryos responding to concentrations of dexamethasone ranging from 10-6 to 10-3M. Furthermore, glucocorticoids which are closely related structural analogs of dexamethasone also disrupt germ band retraction and dorsal closure, while other tested steroids had no effect on embryonic development. At a molecular level, expression of the gene, Krüppel, is absent from the amnioserosa of dexamethasone-treated embryos. The cuticular phenocopy resulting from exposure to dexamethasone and related glucocorticoids is morphologically similar to the mutant phenotype associated with four genes required for germ band retraction, namely hindsight, serpent, tail-up and u-shaped. The results of this study represent the first association of a glucocorticoid with dose, stage and tissue specific effects on Drosophila development at both morphological and molecular levels.","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"204 6","pages":"359-368"},"PeriodicalIF":0.0,"publicationDate":"1995-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00360481","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5