Roux's archives of developmental biology : the official organ of the EDBO最新文献_第3页

Spatial and temporal coincidence of induction processes and gap-junctional communication in Patella vulgata (Mollusca, Gastropoda). 外翻髌骨诱导过程和间隙连接通讯的时空重合(软体动物，腹足动物)。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1996-05-01 DOI: 10.1007/BF00377220

P Damen, W J A G Dictus

{"title":"Spatial and temporal coincidence of induction processes and gap-junctional communication in Patella vulgata (Mollusca, Gastropoda).","authors":"P Damen, W J A G Dictus","doi":"10.1007/BF00377220","DOIUrl":"https://doi.org/10.1007/BF00377220","url":null,"abstract":"In the early development of the gastropod mollusc Patella vulgata, two inductive processes take place between the animal and vegetal hemispheres. One is the induction of the vegetally-located stem cell of the mesoderm (the 3D macromere) by the first-quartet micromeres (which are located in the animal hemisphere). The other is the induction of dorsoventral symmetry in the first-quartet micromeres by the 3D macromere. As a consequence of the latter induction process, a dorsoventrally-organised prototroch is formed. In this paper, the moment of 3D induction is determined by deleting the inducing first-quartet micromeres at successive stages of development. Furthermore, the role of gap junctions in the two above-mentioned induction processes is investigated. This was done by studying lucifer yellow dye transfer between the 3D macromere and the first-quartet micromeres, in relation to these two induction processes. Analysis of the pattern of dye-transfer in vivo, in sections and with confocal laser scanning microscopy demonstrated that the moment of dye transfer coincides with the moment of 3D induction and with the moment of induction of the dorsoventral pattern of the prototroch. This indicates that gap-junctional communication may be involved in these two induction processes.","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"205 7-8","pages":"401-409"},"PeriodicalIF":0.0,"publicationDate":"1996-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00377220","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34821892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Behaviour of centrosomes in early Tubifex embryos: asymmetric segregation and mitotic cycle-dependent duplication. 早期输卵管胚胎中心体的行为：不对称分离和有丝分裂周期依赖的复制。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1996-02-01 DOI: 10.1007/BF00365807

Takashi Shimizu

{"title":"Behaviour of centrosomes in early Tubifex embryos: asymmetric segregation and mitotic cycle-dependent duplication.","authors":"Takashi Shimizu","doi":"10.1007/BF00365807","DOIUrl":"10.1007/BF00365807","url":null,"abstract":"An antibody raised against a highly conserved peptide of γ-tubulin (Joshi et al. 1992) recognized a 50 kDa polypeptide in centrosomes in Tubifex embryos. Centrosomes labelled with this antibody are found at both poles of the first meiotic spindle and at the inner pole of the second meiotic spindle. At the transition to the second meiosis, there is no change in morphology of the centrosomes which are retained in the egg proper. In contrast, as the second meiosis proceeds from anaphase to telophase, centrosomes labelled with the antibody gradually become smaller, but are still recognized as tiny dots; each egg exhibits no more than one tiny dot. The first cleavage spindles exhibit a centrosome at one pole but not at the other. The spindle pole with a centrosome forms an aster which is inherited by the larger cell, CD, of the two-cell embryo; the centrosome-free spindle pole then becomes anastral and is segregated to a smaller cell AB. Centrosomes are present in the C and D cell lineages but not in the A and B lineages, at least up to the eighth cleavage cycle. During cleavage stages, centrosomes duplicate early in telophase of each mitosis, and their size changes in a cell cycle-specific fashion. Centrosomes which otherwise duplicate asynchronously in separate cells do so synchronously in a common cytoplasm. Centrosome duplication is inhibited by nocodazole but not by cytochalasin D. An examination of embryos treated with cycloheximide or aphidicolin also suggests that centrosome duplication during cleavages requires protein synthesis but no DNA replication per se. These results suggest that the centrosome cycle in Tubifex blastomeres is linked to the mitotic cycle more closely than is that in other animals.","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"205 5-6","pages":"290-299"},"PeriodicalIF":0.0,"publicationDate":"1996-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heart formative factor(s) is localized in the anterior endoderm of early Xenopus neurula. 早期非洲爪蟾的心脏形成因子位于前内胚层。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1996-02-01 DOI: 10.1007/BF00365806

Akane Tonegawa, Megumi Moriya, Masazumi Tada, Shinichiro Nishimatsu, Chiaki Katagiri, Naoto Ueno

引用次数: 3

Thyroid hormone-dependent expression of bullfrog tadpole collagenase gene. 甲状腺激素依赖性牛蛙蝌蚪胶原酶基因的表达。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1996-02-01 DOI: 10.1007/BF00365802

Ken Oofusa, Katsutoshi Yoshizato

{"title":"Thyroid hormone-dependent expression of bullfrog tadpole collagenase gene.","authors":"Ken Oofusa, Katsutoshi Yoshizato","doi":"10.1007/BF00365802","DOIUrl":"10.1007/BF00365802","url":null,"abstract":"Collagenase has been suggested to play a pivotal role in tissue remodeling during anuran metamorphosis. RNA blot analysis and nuclear run-on assay showed that collagenase genes of caudal cells of tadpoles of the bullfrog, Rana catesbeiana, are up-regulated by thyroid hormone after only 24 h. The gene for collagenase was cloned from genome DNA of the bullfrog and its structure, including the 5'-upstream region with-1 kbp, was determined. The gene contained 4 exons with sequences of the enzyme active centre in exon 2. Cis-acting regulatory sequences were recognized in the 5'-upstream region as AP-1 and Sp1, which had also been found in the mammalian collagenase gene. A special sequence was noticed near -870 bp, 5'-AG-GTAAGAACAGGATA-3', that satisfies a general criterion for the identification of the thyroid hormone responsive element (TRE) proposed by Umesono et al. (1991) and is homologous to the element reported in genes of thyroid-hormone responsive proteins such as thyroid hormone receptor (TR) β, growth hormone, malic enzyme and myosin heavy chain. Results of the gel mobility shift assay supported the idea that the sequence functions as a TRE. The supershift procedure demonstrated that the complex formed in the gel mobility shift assay contains TR. The present study revealed the uniqueness of bullfrog collagenase as a metamorphosis-associated enzyme, because, to our knowledge, known mammalian collagenase genes do not contain THE in their transcription regulatory regions.","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"205 5-6","pages":"243-251"},"PeriodicalIF":0.0,"publicationDate":"1996-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Subcortical microtubule network separates the peripiasm from the endoplasm and is responsible for maintaining the position of accessory nuclei in hymenopteran oocytes. 在膜翅目卵母细胞中，皮层下微管网络将外周质与内质分离，并负责维持副核的位置。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1996-02-01 DOI: 10.1007/BF00365810

Szczepan M Biliński, Jerzy Klag, Janusz Kubrakiewicz

引用次数: 1

Vitamin A deficiency and the expression of retinoic acid receptors during early cardiogenesis in quail embryo. 鹌鹑胚胎早期心脏发生过程中维生素A缺乏与视黄酸受体的表达。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1996-02-01 DOI: 10.1007/BF00365804

I Kostetskii, K K Linask, M H Zile

{"title":"Vitamin A deficiency and the expression of retinoic acid receptors during early cardiogenesis in quail embryo.","authors":"I Kostetskii, K K Linask, M H Zile","doi":"10.1007/BF00365804","DOIUrl":"https://doi.org/10.1007/BF00365804","url":null,"abstract":"The vitamin A deficiency-associated lethal syndrome observed in avian embryos may be linked to dysfunction of vitamin A-dependent genes. We tested this hypothesis in a quail embryo model by examining the expression of retinoic acid receptors (RARs) and cytosolic retinoic acid binding protein (CRABP) in normal and vitamin A-deficient embryos during early development. RARα and RARγ mRNA were expressed at the same level in normal and vitamin A-deficient embryos during all stages studied. Expression of CRABP I was low in normal and vitamin A-deficient quail embryos during early development, but increased rapidly at later stages. Two transcripts of RARβ, 3.2 and 3.5 kb, were detected in quail embryos during developmental stages 6-12. In normal emryos the level of the 3.2-kb isoform increased as embryonic development advanced. The expression of the 3.5-kb transcript was significantly decreased in vitamin A-deficient embryos, while the 3.2-kb transcript was undetectable by northern analysis. In situ hybridization of stage 7-8 normal quail embryos using a chicken RARβ2 riboprobe revealed that RARβ2 expression was predominantly associated with the cell populations in heart-forming regions, somites, neural folds, notochord and the presumptive thyroid. In stark contrast, in the vitamin A-deficient quail embryo RARβ2 was not expressed in any of the above cell populations. We conclude that the expressions of RARβ and CRABP I are developmentally regulated. Additionally, the expression of RARβ2 during early embryogenesis is regulated by vitamin A status. We propose that RARβ2 plays an important role in the mechanism of action of retinoids in early avian development. The lack of expression of RARβ2 may be linked to abnormalities of the cardiovascular system.","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"205 5-6","pages":"260-271"},"PeriodicalIF":0.0,"publicationDate":"1996-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00365804","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Isolation of Xenopus HGF gene promoter and its functional analysis in embryos and animal caps. 爪蟾HGF基因启动子的分离及其在胚胎和动物帽中的功能分析。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1996-02-01 DOI: 10.1007/BF00365808

Hisashi Nakamura, Kosuke Tashiro, Koichiro Shiokawa

{"title":"Isolation of Xenopus HGF gene promoter and its functional analysis in embryos and animal caps.","authors":"Hisashi Nakamura, Kosuke Tashiro, Koichiro Shiokawa","doi":"10.1007/BF00365808","DOIUrl":"10.1007/BF00365808","url":null,"abstract":"Previously, we isolated Xenopus HGF (hepatocyte growth factor) cDNA and showed in Xenopus embryos that expression of this gene starts at the late gastrula stage mainly in the ventral mesoderm, and furthermore that the expression is induced in animal cap by activin A and bFGF (basic fibroblast growth factor). Here we have cloned the Xenopus HGF gene, covering a 14 kb 5'-upstream region and a 0.2 kb 5'-coding region. Within about 0.5 kb of the 5'-flanking region, the Xenopus HGF gene contained a TATA-like element AATGAAA, one putative NF-1 binding site, two NF-IL-6 binding motif sequences, one putative TGF-β-dependent inhibitory element (TIE) and one AP-1 binding site. A recombinant circular plasmid consisting of a 1.7 kb HGF promoter region and the bacterial chloramphenicol acetyltransferase (CAT) gene was first expressed at the late gastrula stage in the ventral mesoderm, as was the endogenous HGF gene. The expression of the fusion gene was induced in animal cap cells by activin A and bFGF although induction by the latter was not so strong. Using a series of 5'-deletion constructs introduced into animal caps, silencer elements, which seem to be essential for the gene's regionally correct expression, and the element responsible for induction by activin were found. The results show that the HGF gene promoter isolated here contains elements which may endow the gene with the regulative function for its temporally and spatially regulated expression, although the element necessary for induction by bFGF seems to be missing.","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"205 5-6","pages":"300-310"},"PeriodicalIF":0.0,"publicationDate":"1996-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The relationship of innervation and differentiation to regenerative capacity in the reamputated hindlimb of larval Xenopus laevis. 非洲爪蟾断肢幼体神经支配和分化与再生能力的关系。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1996-02-01 DOI: 10.1007/BF00365803

S Bernardini, S M Cannata, G La Mesa, S Filoni

{"title":"The relationship of innervation and differentiation to regenerative capacity in the reamputated hindlimb of larval Xenopus laevis.","authors":"S Bernardini, S M Cannata, G La Mesa, S Filoni","doi":"10.1007/BF00365803","DOIUrl":"https://doi.org/10.1007/BF00365803","url":null,"abstract":"Regenerated hindlimbs of larval Xenopus laevis were reamputated at critical larval stages and levels, viz when amputation of the control limb at the same larval stage and level is followed by reduced regeneration. Reamputations were performed at the level of (1) the original plane of amputation, (2) the early regenerate (cone/palette stage), (3) the late regenerate (digit stage). Reamputation increased both the percentage rate of regeneration and the morphological complexity of the regenerates in all experimental series. Cell counts in lateral motor columns and spinal ganglia innervating the hindlimb, together with histological observations and mitotic index and labelling index determinations in reamputated and control limbs showed that improved regeneration in the reamputated limb was related to an increase in undifferentiated and proliferating cells in the stump. We did not find any evidence suggesting that renewed regeneration in reamputated anuran limbs results from an increase in innervation, as has previously been hypothesized. We support our conclusions by demonstrating an improvement in regenerationen in the reamputated and denervated hindlimbs.","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"205 5-6","pages":"252-259"},"PeriodicalIF":0.0,"publicationDate":"1996-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00365803","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Early axonogenesis in the embryo of a primitive insect, the silverfish Ctenolepisma longicaudata. 原始昆虫Ctenolepisma longicaudata银鱼胚胎的早期轴突发育。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1996-02-01 DOI: 10.1007/BF00365805

Paul M Whitington, Kerri-Lee Harris, David Leach

{"title":"Early axonogenesis in the embryo of a primitive insect, the silverfish Ctenolepisma longicaudata.","authors":"Paul M Whitington, Kerri-Lee Harris, David Leach","doi":"10.1007/BF00365805","DOIUrl":"https://doi.org/10.1007/BF00365805","url":null,"abstract":"The pattern of axon growth from the population of neurons that pioneers the major axon pathways in the central nervous system is highly conserved in winged insects. This study sought to determine whether the same pattern of axon growth is shared by an apterygotic insect, the silverfish. We have found that homologues to at least nine early differentiating winged insect neurons are present in the silverfish. The axon trajectories and the sequence of axon outgrowth from these neurons are very similar in silverfish and winged insects, suggesting that the pterygotic and apterygotic insects share a common developmental Bauplan for the construction of the central nervous system. Some of these neurons do show differences in several aspects of axon growth, including the relative timing of axonogenesis, the polarity of axon growth and the pattern of axon fasciculation. In addition, a major, early-appearing fascicle in the posterior commissure of the silverfish is pioneered by a neuron which does not appear to have an equivalent in the winged insects. These differences are similar in character to, albeit more pronounced than, differences previously reported between two winged insects, the fruitfly Drosophila and the grasshopper. Some of the features of early central axon growth, that set the silverfish embryo apart from the winged insects, are shared by crustacean embryos, providing support for the claim that insects and crustaceans share a common developmental Bauplan for the construction of central axonal pathways.","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"205 5-6","pages":"272-281"},"PeriodicalIF":0.0,"publicationDate":"1996-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00365805","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

LWamides from Cnidaria constitute a novel family of neuropeptides with morphogenetic activity. 来自刺胞菌的LWamides构成了一个具有形态发生活性的神经肽新家族。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1996-02-01 DOI: 10.1007/BF00365801

Martin Gajewski, Thomas Leitz, Jörg Schloßherr, Günter Plickert

{"title":"LWamides from Cnidaria constitute a novel family of neuropeptides with morphogenetic activity.","authors":"Martin Gajewski, Thomas Leitz, Jörg Schloßherr, Günter Plickert","doi":"10.1007/BF00365801","DOIUrl":"https://doi.org/10.1007/BF00365801","url":null,"abstract":"Metamorphosin A (MMA) isolated from the anthozoan Anthopleura elegantissima has recently been shown to interfere with developmental control in the colonial hydroid Hydractinia echinata. In order to identify the functional homologue in this species we have cloned cDNAs of the precursor protein from Hydractinia and, for comparison, precursor sequences from two further anthozoans. The deduced preproproteins contain multiple copies of propeptides to be processed into a great variety of novel neuropeptides most of which are N-terminally different from MMA. Original MMA is only contained in the anthozoan precursors. Most of the novel neuropeptides will have the carboxyl terminus LWamide. Therefore, we term this novel neuropeptide family the LWamides. Peptides synthesized according to the precursor sequence of H. echinata and added to planulae trigger metamorphosis. In contrast, none of 11 other known biologically active peptides including carboxamidated neuropeptides were effective. Expression analysis by in situ hybridization and by antibodies against the H. echinata peptide reveals the presence of the gene product in planulae at the proper time and at the due spatial location expected for a natural role in metamorphosis. LWamide transcripts are also observed in nerve cells of primary and adult polyps, suggesting LWamides to be a multifunctional family of neuropeptides.","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"205 5-6","pages":"232-242"},"PeriodicalIF":0.0,"publicationDate":"1996-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00365801","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 73