Roux's archives of developmental biology : the official organ of the EDBO最新文献_第2页

Editorial. 社论。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1996-05-01 DOI: 10.1007/BF00377211

Diethard Tautz

引用次数: 0

Axis formation in half blastoderms of the chick: Stage at separation and the relative positions of fused halves influence axis development. 鸡胚半胚层中轴的形成:分离阶段和融合半胚层的相对位置影响轴的发育。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1996-05-01 DOI: 10.1007/BF00377216

O Khaner

{"title":"Axis formation in half blastoderms of the chick: Stage at separation and the relative positions of fused halves influence axis development.","authors":"O Khaner","doi":"10.1007/BF00377216","DOIUrl":"https://doi.org/10.1007/BF00377216","url":null,"abstract":"The blastoderm of the avian embryo acts during the early stages of development as an integrative system programmed to form a single embryonic axis. Isolated parts of the blastoderm are known to each form an axis, owing to the system's properties. In the work reported here, the regulative capability of the right and left halves of chick blastoderms to form an embryonic axis was examined systematically at different stages. This revealed a progressive change in the developing blastoderm. After early separation, the axis in each half will form at some distance from the blastoderm's original midline, while with late separation the axis will form next to the original midline and may even lack one row of somites at the medial rim. Since development stops in culture after about 2 days, axis development after early separation ceases before somites are formed, whereas after late separation somites and brain vesicles can develop. In addition, an attempt was made to learn whether the two halves of blastoderm, when shifted along the midline and then reunited in staggered fashion, act as a single or two separate embryonic fields. When reunion of the right and left halves was achieved so that the posterior end of one half was adjoining the posterior area pellucida region of the other half, a single embryonic axis developed. When, on the other hand, the shift was larger so that the posterior end was fused to the central area pellucida of the other half, two separated embryonic axes developed.","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"205 7-8","pages":"364-370"},"PeriodicalIF":0.0,"publicationDate":"1996-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00377216","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34821888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Establishment of self-sterility of eggs in the ovary of the solitary ascidian, Halocynthia roretzi. 孤海鞘卵自育性的建立。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1996-05-01 DOI: 10.1007/BF00377219

M Fuke, T Numakunai

{"title":"Establishment of self-sterility of eggs in the ovary of the solitary ascidian, Halocynthia roretzi.","authors":"M Fuke, T Numakunai","doi":"10.1007/BF00377219","DOIUrl":"10.1007/BF00377219","url":null,"abstract":"When unfertilized eggs (UFE) of the solitary ascidian, Halocynthia roretzi, are released naturally they are strictly self-sterile, whereas almost all ovarian eggs isolated after spawning are self-fertile. Self-sterile eggs are prepared within a relatively short period of several hours before the spawning. The morphological changes in ovarian eggs during late oogenesis were studied with special reference to the establishment of self-sterility. Four types of eggs at serial developmental stages were classified according to the morphology of their external envelopes. Self-sterility was established in the last stage, from the ovarian egg type 3 (OVE3) to UFE stages. Ovarian eggs which had become committed to UFE were denoted as full-grown ovarian eggs (FOE). FOE were able to differentiate into self-sterile UFE in vitro, whereas OVE3 could not. Several morphological differences between OVE3 and UFE were found. OVE3 had a germinal vesicle (GV), a type of vitelline coat (VC-OVE3) and no expanded perivitelline space, whereas UFE had completed germinal vesicle break down (GVBD), had another type of coat (VC-UFE) and showed an expanded perivitelline space. There were also some differences in the mode of fertilization between OVE3 and UFE. In UFE, sperm became bound firmly to the vitelline coat and passed through the coat with the help of follicle cells, whereas in OVE3, sperm did not bind so strongly and entered the perivitelline space without the aid of follicle cells. The relationships between the establishment of self-sterility and these morphological and functional changes in ovarian eggs are discussed.","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"205 7-8","pages":"391-400"},"PeriodicalIF":0.0,"publicationDate":"1996-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34821891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of mesenchymal influence on the pepsinogen gene expression in the epithelium of chicken embryonic digestive tract. 鸡胚消化道上皮间质对胃蛋白酶原基因表达的影响分析。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1996-05-01 DOI: 10.1007/BF00377218

K Urase, K Fukuda, Y Ishii, N Sakamoto, S Yasugi

{"title":"Analysis of mesenchymal influence on the pepsinogen gene expression in the epithelium of chicken embryonic digestive tract.","authors":"K Urase, K Fukuda, Y Ishii, N Sakamoto, S Yasugi","doi":"10.1007/BF00377218","DOIUrl":"10.1007/BF00377218","url":null,"abstract":"We performed tissue recombination experiments to discover the mesenchymal influences on differentiation of epithelia in chicken digestive organs. Epithelia and mesenchymes were taken from the lung, esophagus, proventriculus, gizzard, small intestine and large intestine of 6-day chicken embryos and recombined in various associations and cultivated in vitro for 6 days. Rather unexpectedly, embryonic chicken pepsinogen (ECPg) gene, a marker of the proventricular epithelium, was induced in the gizzard epithelium, which does not express ECPg in normal development, by the proventricular and lung mesenchymes. In the second half of this study, we investigated the mode of action of mesenchymal cells on ECPg expression in gizzard epithelial cells more precisely using the cell aggregate culture system, in which gizzard epithelial cells were mixed with proventricular, gizzard or lung mesenchymal cells. We found that supporting action of lung mesenchymal cells on ECPg expression was even stronger than that of proventricular mesenchymal cells, and suggest that the action of lung mesenchyme may be due partly to the enhancement of epithelial cell proliferation. According to the results of this study, together with many facts obtained so far, we will discuss a new model for restricted expression of ECPg in the proventricular epithelium in normal development.","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"205 7-8","pages":"382-390"},"PeriodicalIF":0.0,"publicationDate":"1996-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34821890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hilde Mangold (1898-1924) and Spemann's organizer: achievement and tragedy. 希尔德·曼戈尔德（1898-1924）和施佩曼的组织者：成就与悲剧。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1996-05-01 DOI: 10.1007/BF00377212

Peter E Fässler, Klaus Sander

引用次数: 0

Muscle regeneration in the holothurian Stichopus japonicus. 海参刺参的肌肉再生。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1996-05-01 DOI: 10.1007/BF00377230

I Y Dolmatov, M G Eliseikina, T T Ginanova, N E Lamash, V P Korchagin, A A Bulgakov

{"title":"Muscle regeneration in the holothurian Stichopus japonicus.","authors":"I Y Dolmatov, M G Eliseikina, T T Ginanova, N E Lamash, V P Korchagin, A A Bulgakov","doi":"10.1007/BF00377230","DOIUrl":"10.1007/BF00377230","url":null,"abstract":"The regeneration of longitudinal muscle bands (LMBs) in the sea cucumber Stichopus japonicus was studied using light and electron microscopic and immunocytochemical methods. Previous investigations of holothurian organs showed the presence of some cytoskeletal proteins which were specific for LMBs only. One of them, the 98 KDa protein, was isolated by means of SDS-electrophoresis and used as an antigen to obtain polyclonal antibodies. When tested on paraffin sections of sea cucumber organs, the antibodies were shown to interact only with coelomic epithelial cells covering the LMBs. The antibodies were used to study LMB regeneration after transverse cutting. During regeneration no signs of myocyte dedifferentiation or mitotic division were observed. In the wound region, damaged myocytes degenerated and muscle bundles desintegrated. However, the coelomic epithelial cells dedifferentiated and began to invade the LMB. Just beneath the surface these cells formed clusters (muscle bundle rudiments). The number and size of the clusters gradually increased, the cells lengthened and developed contractile filaments. These observations suggest that new muscle bundles arise from coelomic epithelial cells covering the LMBs. The migration of coelomic epithelial cells into the damaged LMBs and their myogenic transformation are the basic mechanism of holothurian muscle regeneration.","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"205 7-8","pages":"486-493"},"PeriodicalIF":0.0,"publicationDate":"1996-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stage, tissue, and cell specific distribution of alternative Ultrabithorax mRNAs and protein isoforms in the Drosophila embryo. 在果蝇胚胎中，不同的超胸腔mrna和蛋白亚型的阶段、组织和细胞特异性分布。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1996-05-01 DOI: 10.1007/BF00377226

A J López, R D Artero, M Pérez-Alonso

{"title":"Stage, tissue, and cell specific distribution of alternative Ultrabithorax mRNAs and protein isoforms in the Drosophila embryo.","authors":"A J López, R D Artero, M Pérez-Alonso","doi":"10.1007/BF00377226","DOIUrl":"10.1007/BF00377226","url":null,"abstract":"The homeotic gene Ultrabithorax encodes a family of six homeoproteins translated from alternatively spliced mRNAs. The structures of these UBX isoforms have been conserved among anciently diverged Drosoph-ila species and functional distinctions between some isoforms have been reported that suggest subtle but important roles in Ubx action. We present a detailed analysis of the expression patterns of Ubx mRNAs and proteins during embryogenesis, using isoform-specific monoclonal antibodies and synthetic oligonucleotide probes. These patterns are remarkably complex, each mRNA and corresponding protein isoform being expressed in a partially overlapping but distinct stage and tissue-specific pattern. The complexity is greatest in the central nervous system, where different isoforms predominate during successive developmental stages and where their relative proportions differ from one metamere to another and even among individual neurons within a given metamere. The distributions of UBX isoforms are consistent with those functional distinctions that have been described; they also suggest that different isoforms may be specialized or optimized to control different aspects of central nervous system development. The close correspondence between the mRNA and protein patterns indicates that the mRNAs do not differ strongly in translatability, despite the abundance of rare codons in the optional exons. There is a delay between the detection of particular splicing events in the nucleus and the detection of the 3' end of the message or the appearance of the corresponding mRNAs and proteins in the cytoplasm. This delay is consistent with the size of the Ubx introns and indicates a cotranscriptional mechanism of splicing.","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"205 7-8","pages":"450-459"},"PeriodicalIF":0.0,"publicationDate":"1996-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Embryonic gut differentiation in nematodes: endocytosis of macromolecules and its experimental inhibition. 线虫的胚胎肠分化:大分子的内吞作用及其实验抑制。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1996-05-01 DOI: 10.1007/BF00377231

O Bossinger, O Wiegner, E Schierenberg

{"title":"Embryonic gut differentiation in nematodes: endocytosis of macromolecules and its experimental inhibition.","authors":"O Bossinger, O Wiegner, E Schierenberg","doi":"10.1007/BF00377231","DOIUrl":"https://doi.org/10.1007/BF00377231","url":null,"abstract":"During embryogenesis of Caenorhabditis elegans cytoplasmic components are transferred from nongut cells into the developing gut primordium and an exo/endocytosis mechanism has been hypothesized (Bossinger and Schierenberg 1992). To test endocytotic activity of the gut primordium, we compared the uptake of different fluorochrome-conjugated marker molecules in two nematode species, C. elegans and Cephalobus spec., which differ in the pattern of early cleavage and cell-cell communication. We found no uptake of dextran (as a marker for pinocytosis) but rapid internalization of 30-fold larger transferrin molecules (as a marker for receptor-coupled endocytosis) into the differentiating gut primordium in both nematodes. The two studied species differ with respect to when this process starts. While the uptake of macromolecules in the fast developing C. elegans is first observed at a stage when essentially all cells of the hatching juvenile have been generated, in the slow developing Cephalobus endocytosis begins during the early proliferation phase when only two gut precursor cells are present. We found that the polysulfated hydrocarbon dye trypan blue and the cationic amphiphilic drug chlorpromazine both inhibit endocytosis into the gut primodium.","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"205 7-8","pages":"494-497"},"PeriodicalIF":0.0,"publicationDate":"1996-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00377231","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Spatio-temporal expression of pamlin during early embryogenesis in sea urchin and importance of N-linked glycosylation for the glycoprotein function. 海胆胚胎早期pamlin的时空表达及n -链糖基化对糖蛋白功能的重要性

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1996-05-01 DOI: 10.1007/BF00377217

H Katow, S Komazaki

{"title":"Spatio-temporal expression of pamlin during early embryogenesis in sea urchin and importance of N-linked glycosylation for the glycoprotein function.","authors":"H Katow, S Komazaki","doi":"10.1007/BF00377217","DOIUrl":"10.1007/BF00377217","url":null,"abstract":"Expression of pamlin, a heterotrimeric primary mesenchyme cell (PMC) adhesion glycoprotein, and its role during early embryogenesis were examined using immunochemistry and microinjection of pamlin to tunicamycin-treated embryos of the sea urchin, Hemicentrotus pulcherrimus. Pamlin faintly detected in egg cortex before fertilization was strongly expressed in the hyaline layer after fertilization. The embryonic apical surface retained pamlin throughout early embryogenesis, whereas pamlin on the basal surface showed a dynamic change of spatio-temporal distribution from morula to gastrula stage. Pamlin distributed on the entire basal surface of the ectoderm before onset of invagination gradually disappeared from the presumptive archenteron during gastrulation, and then was restricted to the apical tuft region and the PMC sessile sites in early gastrulae. Tunicamycin, an inhibitor of N-glycosydically linked carbohydrate formation, inhibited PMC migration and gastrulation. Tunicamycin also inhibited the assembly of mannose moieties of 180 and 52 kDa subunits of pamlin. Pamlin microinjection to the tunicamycin-treated embryos rescued them from this morphogenetic disturbance. PMCs did not bind to pamlin isolated from the tunicamycin-treated embryos. The present study indicated that pamlin plays an essential role in PMC migration, its termination and gastrulation, and the presence of N-glycosydically linked carbohydrate moieties that contain mannose are necessary to preserve the biological function of pamlin.","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"205 7-8","pages":"371-381"},"PeriodicalIF":0.0,"publicationDate":"1996-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34821889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Terminal filament cell organization in the larval ovary of Drosophila melanogaster: ultrastructural observations and pattern of divisions. 黑腹果蝇子房末丝细胞组织:超微结构观察及分裂模式。

Roux's archives of developmental biology : the official organ of the EDBO Pub Date : 1996-05-01 DOI: 10.1007/BF00377215

Isabelle Sahut-Barnola, Bernard Dastugue, Jean-Louis Couderc

{"title":"Terminal filament cell organization in the larval ovary of Drosophila melanogaster: ultrastructural observations and pattern of divisions.","authors":"Isabelle Sahut-Barnola, Bernard Dastugue, Jean-Louis Couderc","doi":"10.1007/BF00377215","DOIUrl":"https://doi.org/10.1007/BF00377215","url":null,"abstract":"The adult ovary of Drosophila is composed of approximately twenty parallel repetitive structures called ovarioles. The ovarioles appear at the prepupal stage and their formation requires the presence of stacks of discshaped cells called the terminal filaments. Terminal filaments form in a progressive manner during the third larval instar. We have looked at the beginning of formation of both the terminal filaments and ovarioles at an ultrastructural level. Moreover, we studied the pattern of division of the terminal filament cell precursors using the base analog, BrdU. Two main waves of division are observed. The first wave consists of divisions of almost all the terminal filament cell precursors during a short period of time at the transition between the second and third larval instar. The second wave, in which the precursors carry out their final divisions before differentiating, occurs gradually, going from the medial to the lateral side of the ovary during the first half of the third larval instar.","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"205 7-8","pages":"356-363"},"PeriodicalIF":0.0,"publicationDate":"1996-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00377215","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34821887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24