Roux's archives of developmental biology : the official organ of the EDBO最新文献

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Fate mapping study of the endoderm of the 1.5-day-old chick embryo. 1.5日龄鸡胚内胚层命运定位研究。
Susumu Matsushita
{"title":"Fate mapping study of the endoderm of the 1.5-day-old chick embryo.","authors":"Susumu Matsushita","doi":"10.1007/BF00365800","DOIUrl":"10.1007/BF00365800","url":null,"abstract":"<p><p>Various portions of the endoderm between the levels of the first and the 10th somite of 1.5-day-old chick embryos were marked by local application of the vital dye Dil, and the fate of marked cells was analyzed after cultivation of the embryos for 2 days in vitro.The presumptive area of digestive tract ranging from the posterior pharynx to the jejunum was found to extend bilaterally from the midline of the 1.5-day embryo with a width two or three times as great as the distance between the midline and the lateral edge of the somite. Either side of this area contributed to the same side of the endodermal tube of digestive tract. The anterior and posterior portions generally contributed to the anterior and posterior regions of the digestive tract, respectively, and the cells originating from the portion farther from the midline took the more ventral and posterior position in the digestive tract endoderm. Most of the presumptive areas of the digestive organs in the endoderm of 1.5-day embryo were located in a more anterior position than those in the splanchnic mesoderm.</p>","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"205 5-6","pages":"225-231"},"PeriodicalIF":0.0,"publicationDate":"1996-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The multi sex combs gene of Drosophila melanogaster is required for proliferation of the germline. 黑腹果蝇的多性别梳子基因是种系增殖所必需的。
France Docquier, Olivier Saget, Françoise Forquignon, Neel B Randsholt, Pedro Santamaria
{"title":"The multi sex combs gene of Drosophila melanogaster is required for proliferation of the germline.","authors":"France Docquier,&nbsp;Olivier Saget,&nbsp;Françoise Forquignon,&nbsp;Neel B Randsholt,&nbsp;Pedro Santamaria","doi":"10.1007/BF00365798","DOIUrl":"https://doi.org/10.1007/BF00365798","url":null,"abstract":"<p><p>We present a genetic analysis showing that the Drosophila melanogaster gene multi sex combs (mxc; Santamaria and Randsholt 1995) is needed for proliferation of the germline. Fertility is the feature most easily affected by weak hypomorphic mutations of this very pleiotropic locus. Pole cell formation and early steps of gonadogenesis conform to the wild-type in embryos devoid of zygotic mxc <sup>+</sup> product. mxc mutant gonad phenotypes and homozygous mxc germline clones suggest a role for mxc <sup>+</sup> in control of germ cell proliferation during the larval stages. mxc <sup>+</sup> requirement is germ cell autonomous and specific in females, whilst in males mxc <sup>+</sup> product is also needed in somatic cells of the gonads. Although mxc can be classified among the Polycomb group (Pc-G) of genes, negative trans-regulators of the ANT-C and BX-C gene complexes, germline requirement for mxc appears independent of a need for other Pc-C gene products, and mxc gonad phenotypes are different from those induced by mutations in BX-C genes. We discuss the possible functions of the mxc <sup>+</sup> product which helps to maintain homeotic genes repressed and prevents premature larval haemocyte differentiation and neoplasic overgrowth, but promotes growth and differentiation of male and female gonads.</p>","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"205 5-6","pages":"203-214"},"PeriodicalIF":0.0,"publicationDate":"1996-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00365798","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Morphogenetic movements during axolotl neural tube formation tracked by digital imaging. 用数字成像技术跟踪蝾螈神经管形成过程中的形态发生运动。
G Wayne Brodland, Michael J Scott, Andrew F MacLean, M Globus, S Vethamany-Globus, R Gordon, Jim H Veldhuis, R Del Maestro
{"title":"Morphogenetic movements during axolotl neural tube formation tracked by digital imaging.","authors":"G Wayne Brodland, Michael J Scott, Andrew F MacLean, M Globus, S Vethamany-Globus, R Gordon, Jim H Veldhuis, R Del Maestro","doi":"10.1007/BF00365809","DOIUrl":"10.1007/BF00365809","url":null,"abstract":"<p><p>During neurulation in vertebrate embryos, epithelial cells of the neural plate undergo complex morphogenetic movements that culminate in rolling of the plate into a tube. Resolution of the determinants of this process requires an understanding of the precise movements of cells within the epithelial sheet. A computer algorithm that allows automated tracking of epithelial cells visible in digitized video images is presented. It is used to quantify the displacement field associated with morphogenetic movements in the axolotl (Ambystoma mexicanum) neural plate during normal neural tube formation. Movements from lateral to medial, axial elongations and area changes are calculated from the displacement field data and plotted as functions of time. Regional and temporal differences are identified. The approach presented is suitable for analyzing a wide variety of morphogenetic movements.</p>","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"205 5-6","pages":"311-318"},"PeriodicalIF":0.0,"publicationDate":"1996-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetic interactions of pokkuri with seven in absentia, tramtrack and downstream components of the sevenless pathway in R7 photoreceptor induction in Drosophila melanogaster. 黑腹果蝇R7光感受器诱导中7缺失途径及其下游组分的遗传互作
Daisuke Yamamoto, Itsuko Nihonmatsu, Takashi Matsuo, Hiroshi Miyamoto, Shunzo Kondo, Kanako Hirata, Yuko Ikegami
{"title":"Genetic interactions of pokkuri with seven in absentia, tramtrack and downstream components of the sevenless pathway in R7 photoreceptor induction in Drosophila melanogaster.","authors":"Daisuke Yamamoto,&nbsp;Itsuko Nihonmatsu,&nbsp;Takashi Matsuo,&nbsp;Hiroshi Miyamoto,&nbsp;Shunzo Kondo,&nbsp;Kanako Hirata,&nbsp;Yuko Ikegami","doi":"10.1007/BF00365799","DOIUrl":"https://doi.org/10.1007/BF00365799","url":null,"abstract":"<p><p>The sevenless (sev) cascade plays an inductive role in formation of the R7 photoreceptor, whilst the pokkuri (pok) and tramtrack (ttk) gene products are known to repress R7 induction in developing ommatidia of Drosophila melanogaster. To elucidate how these positive and negative signalling mechanisms co-operate in the normal fate determination of R7, genetic interactions of mutations in the pok locus with ttk and downstream elements of sev including Gap1, raf1, rolled (r1) and seven in absentia (sina) were examined. The eye phenotype of a weak hypomorph, pok <sup>15</sup>, was enhanced dominantly by Gap1-<sup>mip</sup>, a recessive mutation in a gene encoding a down-regulator of Ras1, producing multiple R7 in ommatidia. Ras1 has been reported to activate r1-encoded mitrogen-activated protein (MAP) kinase via Raf1 that is associated physically with Rasl. Ommatidia of raf1 <sup>c110</sup> and rl <sup>2</sup>/rl<sup>EMS64</sup> typically lacked R7 and a few outer photoreceptors. The pok <sup>1</sup> mutation suppressed dominantly the rafl <sup>c110</sup> rl<sup>2</sup>/rl<sup>EMS64</sup> eye phenotypes, allowing single R7 cells to develop in ommatidia. The rafl <sup>c110</sup> mutation improved adult viability of pok <sup>1</sup> homozygotes. An in vitro experiment demonstrated that MAP kinase phosphorylates Pok protein. Ttk is a transcriptional repressor which binds to the regulatory sequence upstream of the fushi-tarazu (ftz), even skipped (eve) and engrailed (en) coding region. A reduced activity in ttk resulted in enhancement of the pok phenotype. ttk mutations produced extra R7 cells even in sina homozygotes whilst the pok mutation did not. This result indicates that Ttk represses R7 induction downstream of the sites where Pok and Sina function.</p>","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"205 5-6","pages":"215-224"},"PeriodicalIF":0.0,"publicationDate":"1996-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00365799","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Establishment and movement of egg regions revealed by the size class of yolk platelets in Xenopus laevis. 卵黄血小板大小分类揭示非洲爪蟾卵区建立和运动。
Hiroshi Imoh
{"title":"Establishment and movement of egg regions revealed by the size class of yolk platelets in Xenopus laevis.","authors":"Hiroshi Imoh","doi":"10.1007/BF00357759","DOIUrl":"10.1007/BF00357759","url":null,"abstract":"<p><p>Sizes of yolk platelets were measured in sections of oocytes and embryos in Xenopus. It was found that the average size of the largest group of platelets in cells differed between germ layers of neurulae. It was small (3 to 5 μm) in the ectoderm, medium-sized (5 to 8 µm) in the mesoderm, and large (over 8 μm) in the endoderm. Platelets of these size classes formed layers in egg, the yolk gradient, by the end of oocyte maturation. The yolk gradient contained products of the mitochondrial cloud and a part of the germinal vesicle material at certain positions. The layers of small, medium and large platelets in the egg changed their locations to distribute to the ectoderm, mesoderm and endoderm of neurulae, respectively. The yolk layers in the egg thus represented different prospective fates, and a figure describing the locations of these layers could be regarded as a fate map for the one-cell stage. Most of the marginal blastomeres of embryos at cleavage stages consisted of a few parts with different prospective fates. Results were discussed with reference to available fate maps for cleavage stage embryos.</p>","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"205 3-4","pages":"128-137"},"PeriodicalIF":0.0,"publicationDate":"1995-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34823084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multiple, alternative cleavage patterns precede uniform larval morphology during normal development of Dreissena polymorpha (Mollusca, Lamellibranchia). 在多形软体动物(软体动物,板鳃目)的正常发育过程中,多种不同的卵裂模式先于统一的幼虫形态。
C M Luetjens, A W C Dorresteijn
{"title":"Multiple, alternative cleavage patterns precede uniform larval morphology during normal development of Dreissena polymorpha (Mollusca, Lamellibranchia).","authors":"C M Luetjens, A W C Dorresteijn","doi":"10.1007/BF00357760","DOIUrl":"10.1007/BF00357760","url":null,"abstract":"<p><p>In this study we reinvestigate the early development of the freshwater mussel Dreissena polymorpha, previously studied by Meisenheimer (1901). The data include video time-lapse recordings of living embryos and bisbenzimide stains of fixed embryos as well as morphometry on fixed, serially-sectioned embryos. We present the cell lineage and cell cycle durations up to the first indication of symmetrization within this embryo. We show that early cell cycles last approximately 1h. A dramatic extension of cell cycle duration and a concomitant asynchrony among the various cell lines was observed starting at the fifth cleavage. Short cell cycles, like those of early blastomeres, were a constant property of the largest descendants of the 2d-cell line only. In contrast to Meisenheimer's observations and our experiences with other spiralian embryos, the cleavage pattern proved to follow multiple alternatives. The embryonic quadrants A-D were arranged in either a clockwise or counter-clockwise fashion and the chirality of the third cleavage was either dextral or sinistral irrespective of the arrangement of the quadrants. As a consequence, four different blastomere configurations were encountered and the dorsoventral axis could take four different angles with respect to the plane of first cleavage. The dorsal side was most easily recognized by the position of the 2d-micromere at the 16-cell stage. The fact that all of such embryos could develop into normal, uniform larvae is interpreted as the result of cell-cell interactions in morphogenetic regulation.</p>","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"205 3-4","pages":"138-149"},"PeriodicalIF":0.0,"publicationDate":"1995-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Retinoic acid in the anteroposterior patterning of the zebrafish trunk. 斑马鱼躯干前后图案中的视黄酸。
Nicholas Marsh-Armstrong, Peter McCaffery, George Hyatt, Laura Alonso, John E Dowling, Walter Gilbert, Ursula C Dräger
{"title":"Retinoic acid in the anteroposterior patterning of the zebrafish trunk.","authors":"Nicholas Marsh-Armstrong, Peter McCaffery, George Hyatt, Laura Alonso, John E Dowling, Walter Gilbert, Ursula C Dräger","doi":"10.1007/BF00357756","DOIUrl":"10.1007/BF00357756","url":null,"abstract":"<p><p>Retinoic acid has been linked to pattern formation in the vertebrate anteroposterior axis. This report describes the spatial and temporal distributions of both endogenous retinoic acid and retinoic acid synthase activity along the anteroposterior axis of neurulating zebrafish embryos, as detected by a transient transgenic assay and by a zymography bioassay. Both retinoic acid levels and synthase activity were found to be highest in anterior regions of the trunk at all of the stages which were analysed. The drug disulfiram inhibited retinoic acid synthase activity in the zebrafish trunk both in vitro and in vivo, and reduced retinoic acid levels in vivo. Disulfiram treatment of neurulating embryos resulted in larvae with hypertrophic wavy notochords, shortened spinal cords and deformed pectoral fins. The results support the hypothesis that retinoic acid plays a role in the coordination of axial patterning at the developing node/zone of involution, as well as in the subsequent development of anterior trunk structures such as the fins.</p>","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"205 3-4","pages":"103-113"},"PeriodicalIF":0.0,"publicationDate":"1995-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34823081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of mono- and divalent cations on polyp morphogenesis in isolated tentacles of Aurelia aurita(Scyphozoa). 单价和二价阳离子对水螅离体触须息肉形成的影响。
Andrea Hujer, Georgia Elizabeth Lesh-Laurie
{"title":"Effect of mono- and divalent cations on polyp morphogenesis in isolated tentacles of Aurelia aurita(Scyphozoa).","authors":"Andrea Hujer,&nbsp;Georgia Elizabeth Lesh-Laurie","doi":"10.1007/BF00357758","DOIUrl":"https://doi.org/10.1007/BF00357758","url":null,"abstract":"<p><p>Tentacles excised from syphistoma polyps of Aurelia aurita undergo rapid regeneration to form whole polyps following exposure to an excess or absence of specific ions. It has been shown that a 12-18 h exposure of isolated tentacles to 58 mM excess of Cs<sup>+</sup> results in a rapid firing of nematocysts, followed by an accelerated, synchronous polyp morphogenesis. Absence of Mgt<sup>2+</sup> from the culture solution for 4-24 h also led to an accelerated, synchronous polyp regeneration. In either experimental set-up, incubation in 5-10 mM hydroxyurea effectively halted regeneration. Exposure to an excess of Li<sup>+</sup> (50-200 mm) or K<sup>+</sup> (10-50 mM) caused no firing of nematocysts and a percentage of polyp regeneration only slightly higher than control tentacles. Use of the K<sup>+</sup> channel blocker tetraethylammonium (TEA; 100-300 mM) lead to similar levels of regeneration. A Ca<sup>2+</sup> or K<sup>+</sup>-reduced artificial culture solution did not enhance regeneration. Ouabain (1 mM) dampened the Cs<sup>+</sup> induced acceleration of polyp morphogenesis, and when given without Cs<sup>+</sup>, elicited a control level response.</p>","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"205 3-4","pages":"122-127"},"PeriodicalIF":0.0,"publicationDate":"1995-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00357758","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34823083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Reorganisation of microtubule arrays in the telotrophic ovaries of hemipteran insects: Correlation with meiotic reinitiation. 半足类昆虫远营养卵巢中微管阵列的重组:与减数分裂再起始的关系。
Jonathan D Lane, Howard Stebbings
{"title":"Reorganisation of microtubule arrays in the telotrophic ovaries of hemipteran insects: Correlation with meiotic reinitiation.","authors":"Jonathan D Lane,&nbsp;Howard Stebbings","doi":"10.1007/BF00357761","DOIUrl":"https://doi.org/10.1007/BF00357761","url":null,"abstract":"<p><p>We have shown that, in the ovaries of hemipteran insects, microtubule reorganisation and depolymerisation in the oocytes, and in the nutritive tubes supplying them, correlate with the activation of M-phase promoting factor (MPF) and mitogen-activated protein kinase (MAP kinase) as the oocytes proceed to arrest at the first meiotic metaphase. The application, however, of Xenopus egg extracts with high MPF activities to isolated nutritive tube microtubules failed to result in their depolymerisation, suggesting that a novel factor may be required for the breakdown of these highly stable microtubule arrays in vivo.</p>","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"205 3-4","pages":"150-159"},"PeriodicalIF":0.0,"publicationDate":"1995-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00357761","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
The development of cnidarian stinging cells: maturation and migration of stenoteles of Hydra vulgaris. 刺胞细胞的发育:水螅小管的成熟和迁移。
Jakob Weber
{"title":"The development of cnidarian stinging cells: maturation and migration of stenoteles of Hydra vulgaris.","authors":"Jakob Weber","doi":"10.1007/BF00357763","DOIUrl":"https://doi.org/10.1007/BF00357763","url":null,"abstract":"<p><p>The late stages of stenotele development and the migration and installation of freshly matured stenoteles in Hydra have been studied by kinetic and immunofluorescence investigations with rhodamine-labelled polyps. It was found that the high concentration of osmotic pressure-generating poly(γ-glutamic acid)s is synthesised exclusively within the lumen of the immature nematocyst. Assembly of the polymers, which is completed after approximately 0.5 days, is accompanied by a swelling of the capsule and ends when the cyst is mature. Active migration of the stenoteles to the tentacles begins only about 1.0 day later, and the time required for installation of a stenotele on the outer surface of the tissue amounts to about another 1.5 days. Furthermore, the results obtained suggest that the disintegration of the clusters of growing stenoteles, which begins 0.5 to 1.0 days before maturation, is a passive process; the ability of a nematocyte to migrate actively to the tentacles is acquired after maturation and might be controlled directly by regulating factors contained in the tissue.</p>","PeriodicalId":91963,"journal":{"name":"Roux's archives of developmental biology : the official organ of the EDBO","volume":"205 3-4","pages":"171-181"},"PeriodicalIF":0.0,"publicationDate":"1995-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF00357763","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34822519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
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