Botanical Studies最新文献

{"title":"Uncovering waterlogging-responsive genes in cucumber through machine learning and differential gene correlation analysis.","authors":"Zahra Zinati, Leyla Nazari, Ali Niazi","doi":"10.1186/s40529-024-00433-z","DOIUrl":"10.1186/s40529-024-00433-z","url":null,"abstract":"<p><p>As climate change intensifies, the frequency and severity of waterlogging are expected to increase, necessitating a deeper understanding of the cucumber response to this stress. In this study, three public RNA-seq datasets (PRJNA799460, PRJNA844418, and PRJNA678740) comprising 36 samples were analyzed. Various feature selection algorithms including Uncertainty, Relief, SVM (Support Vector Machine), Correlation, and logistic least absolute shrinkage, and selection operator (LASSO) were performed to identify the most significant genes related to the waterlogging stress response. These feature selection techniques, which have different characteristics, were used to reduce the complexity of the data and thereby identify the most significant genes related to the waterlogging stress response. Uncertainty, Relief, SVM, Correlation, and LASSO identified 4, 4, 10, 21, and 13 genes, respectively. Differential gene correlation analysis (DGCA) focusing on the 36 selected genes identified changes in correlation patterns between the selected genes under waterlogged versus control conditions, providing deeper insights into the regulatory networks and interactions among the selected genes. DGCA revealed significant changes in the correlation of 13 genes between control and waterlogging conditions. Finally, we validated 13 genes using the Random Forest (RF) classifier, which achieved 100% accuracy and a 1.0 Area Under the Curve (AUC) score. The SHapley Additive exPlanations (SHAP) values clearly showed the significant impact of LOC101209599, LOC101217277, and LOC101216320 on the model's predictive power. In addition, we employed the Boruta as a wrapper feature selection method to further validate our gene selection strategy. Eight of the 13 genes were common across the four feature weighting algorithms, LASSO, DGCA, and Boruta, underscoring the robustness and reliability of our gene selection strategy. Notably, the genes LOC101209599, LOC101217277, and LOC101216320 were among genes identified by multiple feature selection methods from different categories (filtering, wrapper, and embedded). Pathways associated with these specific genes play a pivotal role in regulating stress tolerance, root development, nutrient absorption, sugar metabolism, gene expression, protein degradation, and calcium signaling. These intricate regulatory mechanisms are crucial for cucumbers to adapt effectively to waterlogging conditions. These findings provide valuable insights for uncovering targets in breeding new cucumber varieties with enhanced stress tolerance.</p>","PeriodicalId":9185,"journal":{"name":"Botanical Studies","volume":"65 1","pages":"25"},"PeriodicalIF":3.4,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11324642/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141975109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Camellia neriifolia and Camellia ilicifolia (Theaceae) as separate species: evidence from morphology, anatomy, palynology, molecular systematics.","authors":"Zhaohui Ran, Zhi Li, Xu Xiao, Ming Tang","doi":"10.1186/s40529-024-00430-2","DOIUrl":"10.1186/s40529-024-00430-2","url":null,"abstract":"<p><strong>Background: </strong>The systematic status of sect. Tuberculata and its taxonomy have recently attracted considerable attention. However, the different bases for defining the characteristics of sect. Tuberculata has led to many disagreements among the plants in this group. Camellia neriifolia and Camellia ilicifolia have been the subject of taxonomic controversy and have been treated as different species or varieties of the same species. Therefore, it is important to use multiple methods, i.e., integrative taxonomy, to determine the taxonomic status of C. neriifolia and C. ilicifolia. This is the first study to systematically explore the taxonomic position of these two plants on the basis of Morphology, Anatomy, Palynology and Molecular Systematics.</p><p><strong>Results: </strong>Extensive specimen reviews and field surveys showed that many differences exist in C. neriifolia and C. ilicifolia, such as the number of trunk (heavily debarked vs. slightly peeling), leaf type (smooth thin leathery, shiny vs. smooth leathery, obscure or slightly shiny), leaf margin (entire vs. serrate), flower type (subsessile vs. sessile), number of styles (3-4 vs. 3), and sepal (ovate vs. round). Moreover, C. neriifolia has a more distinctive faint yellow flower color, and trunk molting was more severe in C. neriifolia than that in C. ilicifolia. In addition, micromorphological analysis of the leaf epidermis showed that the two species differed in the anticlinal wall, stomatal apparatus, and stomatal cluster, and pollen morphology analyses based on pollen size, germination furrow, and polar and equatorial axes showed that they are both distinct from each other. The results of the phylogenetic tree constructed based on the whole chloroplast genome, protein-coding genes, and ITS2 showed that both C. ilicifolia and C. neriifolia were clustered in different branches and gained high support.</p><p><strong>Conclusions: </strong>The results combine morphology, anatomy, palynology, and molecular systematics to treat both C. neriifolia and C. ilicifolia as separate species in the sect. Tuberculata, and the species names continue to be used as they were previously. In conclusion, clarifying the taxonomic status of C. neriifolia and C. ilicifolia deepens our understanding of the systematic classification of sect. Tuberculata.</p>","PeriodicalId":9185,"journal":{"name":"Botanical Studies","volume":"65 1","pages":"23"},"PeriodicalIF":3.4,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11266325/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141747365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An independent Taiwanese lineage of powdery mildew on the endemic host species Koelreuteria henryi.","authors":"Yu-Wei Yeh, Roland Kirschner","doi":"10.1186/s40529-024-00431-1","DOIUrl":"10.1186/s40529-024-00431-1","url":null,"abstract":"<p><strong>Background: </strong>Powdery mildews (Erysiphaceae, Ascomycota) are common plant disease agents and also cause stress for forest and fruit trees worldwide as well as in Taiwan. The powdery mildew Erysiphe bulbouncinula on Koelreuteria host trees was considered an endemic species in China. While in China the host was K. paniculata and only the teleomorph stage found, the anamorph and the teleomorph were both recorded for the host in Taiwan, K. henryi. We aimed to clarify the relationship of the powdery mildews recorded under E. bulbouncinula with an apparently disjunct distribution.</p><p><strong>Results: </strong>Specimens of powdery mildew on K. henryi from Taiwan were characterized based on the anamorph morphology and DNA sequences. They revealed a new record of Sawadaea koelreuteriae for this host species and Taiwan and a new species of Erysiphe, E. formosana, sister to E. bulbouncinula from China.</p><p><strong>Conclusions: </strong>In Erysiphe on Koelreuteria hosts, speciation of plant parasitic fungi seems to be correlated with disjunct host and geographic distribution possibly shaped by extinction of potential host species which are known only as fossils. Two of the three extant East Asian species of Koelreuteria are now known as hosts of specific Erysiphe species. We may predict a further not yet discovered Erysiphe species on the third East Asian species, K. bipinnata, in South and Southwest China. In the speciation in Sawadaea, the extinction events in Koelreuteria can be excluded from being involved.</p>","PeriodicalId":9185,"journal":{"name":"Botanical Studies","volume":"65 1","pages":"22"},"PeriodicalIF":3.4,"publicationDate":"2024-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11264589/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141723088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New perspectives, additions, and amendments to plant endemism in a North African flora.","authors":"Monier Abd El-Ghani, Hasnaa Hosni, Eman Shamso, Faten Ellmouni","doi":"10.1186/s40529-024-00428-w","DOIUrl":"10.1186/s40529-024-00428-w","url":null,"abstract":"<p><strong>Background: </strong>Endemism is essential in biodiversity, biogeography, and conservation tasks. Based on herbarium specimens kept in some local herbaria, many published literature, and available information, we compiled a comprehensive list and an updated assessment of the Egyptian endemic and near-endemic taxa. The application of quantitative approaches to the distribution patterns, conservation status, and habitat preference of endemic taxa in Egypt was provided. Comparisons of the near-endemic taxa with other neighbouring flora were explained. For each taxon, the distribution patterns, most preferable habitat, biological spectrum, and taxa among 14 phytogeographical regions (Operational Geographical Units; OGUs) of Egypt were determined.</p><p><strong>Results: </strong>In this study, 19 endemics (out of 70) and 76 near-endemics (out of 181) are newly added taxa. Differentiation indices represented the taxonomic degrees of differentiation among endemic taxa. Two different indices were used to assess endemism: single-region endemic taxa (SRET) and multiple-region endemic taxa (MRET). Most endemic and near-endemic taxa were recorded from the mountainous Sinai (S) and the Mareotis sector of the Mediterranean coastal land (Mm). Generally, the most represented families in endemic and near-endemic areas were Asteraceae, Caryophyllaceae, Lamiaceae, and Fabaceae. More than 60% of the endemic taxa occurred in the sandy plains, wadis (desert valleys), and rocky plains and mountains. Applying hierarchical cluster analysis to the occurrences of 70 endemic taxa in the 14 studied OGUs revealed five main floristic groups (I-V), each characterized by certain OGUs. We provided eight groups of near-endemic taxa that represented their extension in neighbouring countries.</p><p><strong>Conclusions: </strong>The presented data will help to fill the gap in our knowledge of endemism, provide baseline information to understand biogeographical processes and facilitate further cooperation toward conservation purposes.</p>","PeriodicalId":9185,"journal":{"name":"Botanical Studies","volume":"65 1","pages":"21"},"PeriodicalIF":3.4,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11252113/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141619381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding the role of magnetic (Fe₃O₄) nanoparticle to mitigate cadmium stress in radish (Raphanus sativus L.).","authors":"Amina Aslam, Zahra Noreen, Madiha Rashid, Muhammad Aslam, Tanveer Hussain, Afifa Younas, Sajid Fiaz, Kotb A Attia, Arif Ahmed Mohammed","doi":"10.1186/s40529-024-00420-4","DOIUrl":"10.1186/s40529-024-00420-4","url":null,"abstract":"<p><p>Heavy metals stress particularly cadmium contamination is hotspot among researchers and considered highly destructive for both plants and human health. Iron is examined as most crucial element for plant development, but it is available in inadequate amount because they are present in insoluble Fe³⁺ form in soil. Fe₃O₄ have been recently found as growth promoting factor in plants. To understand, a sand pot experiment was conducted in completely randomized design (control, cadmium, 20 mg/L Fe₃O₄ nanoparticles,40 mg/L Fe₃O₄ nanoparticles, 20 mg/L Fe₃O₄ nanoparticles + cadmium, 40 mg/L Fe₃O₄ nanoparticles + cadmium) to study the mitigating role of Fe₃O₄ nanoparticles on cadmium stress in three Raphanus sativus cultivars namely i.e., MOL SANO, MOL HOL PARI, MOL DAQ WAL. The plant growth, physiological and biochemical parameters i.e.,shoot length, shoot fresh weight, shoot dry weight, root length, root fresh and dry weight, MDA content, soluble protein contents, APX, CAT, POD activities and ion concentrations, membrane permeability, chlorophyll a, chlorophyll b and anthocyanin content, respectively were studied. The results displayed that cadmium stress remarkably reduces all growth, physiological and biochemical parameters for allcultivars under investigation. However, Fe₃O₄ nanoparticles mitigated the adverse effect of cadmium by improving growth, biochemical and physiological attributes in all radish cultivars. While, 20 mg/L Fe₃O₄ nanoparticles have been proved to be more useful against cadmium stress. The outcome of present investigation displayed that Fe₃O₄ nanoparticles can be utilized for mitigating heavy metal stress.</p>","PeriodicalId":9185,"journal":{"name":"Botanical Studies","volume":"65 1","pages":"20"},"PeriodicalIF":3.4,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11245460/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141589580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogenomics identifies parents of naturally occurring tetraploid bananas.","authors":"Yu-En Lin, Hui-Lung Chiu, Chung-Shien Wu, Shu-Miaw Chaw","doi":"10.1186/s40529-024-00429-9","DOIUrl":"10.1186/s40529-024-00429-9","url":null,"abstract":"<p><strong>Background: </strong>Triploid bananas are almost sterile. However, we succeeded in harvesting seeds from two edible triploid banana individuals (Genotype: ABB) in our conservation repository where various wild diploid bananas were also grown. The resulting rare offspring survived to seedling stages. DNA content analyses reveal that they are tetraploid. Since bananas contain maternally inherited plastids and paternally inherited mitochondria, we sequenced and assembled plastomes and mitogenomes of these seedlings to trace their hybridization history.</p><p><strong>Results: </strong>The coding sequences of both organellar genomic scaffolds were extracted, aligned, and concatenated for constructing phylogenetic trees. Our results suggest that these tetraploid seedlings be derived from hybridization between edible triploid bananas and wild diploid Musa balbisiana (BB) individuals. We propose that generating female triploid gametes via apomeiosis may allow the triploid maternal bananas to produce viable seeds.</p><p><strong>Conclusions: </strong>Our study suggests a practical avenue towards expanding genetic recombination and increasing genetic diversity of banana breeding programs. Further cellular studies are needed to understand the fusion and developmental processes that lead to formation of hybrid embryos in banana reproduction, polyploidization, and evolution.</p>","PeriodicalId":9185,"journal":{"name":"Botanical Studies","volume":"65 1","pages":"19"},"PeriodicalIF":3.4,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11245450/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141589579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating local plant species for effective fall armyworm management strategies in Taiwan.","authors":"Yuan-Ching Tsai, Pei-Qi Luo, Chang-Lin Sung, Yi Li, Fang-Yu Hu, Chih-Lu Wang, Yi-Ning Chen, Ju-Hsin Hsu, Chien-En Liao, Shyh-Rong Chang, Wen-Po Chuang","doi":"10.1186/s40529-024-00424-0","DOIUrl":"10.1186/s40529-024-00424-0","url":null,"abstract":"<p><strong>Background: </strong>The emergence of Spodoptera frugiperda (fall armyworm; FAW) in the world has raised concerns regarding its impact on crop production, particularly on corn and sorghum. While chemical control and Bt crops have been effective in managing FAW damage, the development of pesticide-resistant and Bt-resistant strains necessitates alternative control methods. The push-pull farming system has gained attention, but direct utilization of African plant species in Taiwan faces challenges due to invasive potential and climatic disparities. Therefore, identifying and evaluating suitable local plant species, such as Napier grass (Pennisetum purpureum), Desmodium species, and signal grass (Brachiaria brizantha), is crucial for implementing effective FAW management strategies in Taiwan.</p><p><strong>Results: </strong>In screening fifty Napier grass germplasms, all demonstrated an antibiotic effect, reducing leaf consumption compared to corn. Notably, thirty-five germplasms exhibited robust antibiotic traits, decreasing FAW consumption and increasing mortality rates. Three Napier grass germplasms also attracted more female moths for oviposition. Further evaluation of selected Napier grass germplasms and signal grass demonstrated efficacy in reducing FAW larval weight and survival duration. Additionally, Desmodium species, particularly D. uncinatum, showed promising toxicity against FAW larvae.</p><p><strong>Conclusion: </strong>Our findings support the effectiveness of selected Napier grass germplasms and signal grass as pull plants, and highlight the potential of D. uncinatum as a push plant in FAW management strategies in Taiwan.</p>","PeriodicalId":9185,"journal":{"name":"Botanical Studies","volume":"65 1","pages":"18"},"PeriodicalIF":3.4,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11239643/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141589578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beneficial role of Coronatine on the morphological and physiological responses of Cress Plants (Lepidium sativum) exposed to Silver Nanoparticle.","authors":"Shahla Hashemi Shahraki, Fereshteh Mohamadhasani Javar, Babak Jamali, Fatemeh Sargazi","doi":"10.1186/s40529-024-00425-z","DOIUrl":"10.1186/s40529-024-00425-z","url":null,"abstract":"<p><strong>Background: </strong>Silver nanoparticles are widely used in various fields such as industry, medicine, biotechnology, and agriculture. However, the inevitable release of these nanoparticles into the environment poses potential risks to ecosystems and may affect plant productivity. Coronatine is one of the newly identified compounds known for its beneficial influence on enhancing plant resilience against various stress factors. To evaluate the effectiveness of coronatine pretreatment in mitigating the stress induced by silver nanoparticles on cress plants, the present study was carried out.</p><p><strong>Results: </strong>Our findings indicated a decrease in multiple growth parameters, proline content, chlorophyll a, chlorophyll b, total chlorophyll, and carotenoids in cress plants exposed to silver nanoparticle treatment. This decline could be attributed to the oxidative stress induced by the presence of silver nanoparticles in the plants. Conversely, when coronatine treatment was applied, it effectively mitigated the reduction in growth parameters and pigments induced by the silver nanoparticles. Furthermore, we observed an increase in silver content in both the roots and shoot portions, along with elevated levels of malondialdehyde (MDA) content, hydrogen peroxide (H₂O₂), anthocyanins, glutathione (GSH), and antioxidant enzyme activities in plants exposed to silver nanoparticles. Concurrently, there was a decrease in total phenolic compounds, ascorbate, anthocyanins, and proline content. Pre-treatment of cress seeds with coronatine resulted in increased levels of GSH, total phenolic compounds, and proline content while reducing the silver content in both the root and shoot parts of the plant.</p><p><strong>Conclusions: </strong>Coronatine pre-treatment appeared to enhance both enzymatic and non-enzymatic antioxidant activities, thereby alleviating oxidative stress and improving the response to stress induced by silver nanoparticles.</p>","PeriodicalId":9185,"journal":{"name":"Botanical Studies","volume":"65 1","pages":"17"},"PeriodicalIF":3.4,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11236835/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141562690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic variation induced by a low concentration of ethyl methanesulfonate (EMS) in quinoa 'Longli-4' variety.","authors":"Xiaofeng Li, Xiaoyun Cui, Ruilan Ran, Guoxiong Chen, Pengshan Zhao","doi":"10.1186/s40529-024-00427-x","DOIUrl":"10.1186/s40529-024-00427-x","url":null,"abstract":"<p><p>Quinoa (Chenopodium quinoa, 2n = 4x = 36), a super pseudocereal crop, has been introduced into China nearly 60 years. Many excellent varieties have been developed through massive selection; however, few are developed through mutagenesis breeding. In this study, the 'Longli-4' variety, locally cultivated in Gansu province, Northwest China, was selected for experimentation. The grains of 'Longli-4' were treated with ethyl methanesulfonate (EMS) at a concentration of 0.8% for 8 h. Nine plants from independent M₂ families were randomly selected to investigate the mutagenesis effect of EMS on the quinoa genome. The results indicated that the single nucleotide polymorphisms (SNPs) induced by EMS were unevenly distributed across all 18 chromosomes, with an average mutation frequency of 91.2 SNPs/Mb, ranging from 4.5 to 203.5 SNPs/Mb. A significant positive correlation between the number of SNPs and chromosome length was identified through linear model analysis. Transitions from G/C to A/T were the most predominated in all variant categories, accounting for 34.4-67.2% of the mutations, and SNPs were significantly enriched in intergenic regions, representing 69.2-75.1% of the total mutations. This study provides empirical support for the application of low concentration EMS treatment in quinoa breeding.</p>","PeriodicalId":9185,"journal":{"name":"Botanical Studies","volume":"65 1","pages":"15"},"PeriodicalIF":3.4,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11226418/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141533582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exogenous ethephon treatment on the biosynthesis and accumulation of astragaloside IV in Astragalus membranaceus Bge. Var. Mongholicus (Bge.) Hsiao.","authors":"Haonan Wu, Hang Cui, Yu Tian, Jiawen Wu, Zhenqing Bai, Xiujuan Zhang","doi":"10.1186/s40529-024-00426-y","DOIUrl":"10.1186/s40529-024-00426-y","url":null,"abstract":"<p><strong>Background: </strong>Astragaloside IV is a main medicinal active ingredient in Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao, which is also the key biomarker of A. membranaceus quality. Ethylene has been well-documented to involve in secondary metabolites biosynthesis in plants. Nevertheless, how ethylene regulates astragaloside IV biosynthesis in A. membranaceus is still unclear. Therefore, in the present study different dosages and time-dependent exogenous application of ethephon (Eth) were employed to analyze astragaloside IV accumulation and its biosynthesis genes expression level in hydroponically A. membranaceus.</p><p><strong>Results: </strong>Exogenous 200 µmol·L^- 1Eth supply is most significantly increased astragaloside IV contents in A. membranaceus when compared with non-Eth supply. After 12 h 200 µmol·L^- 1 Eth treatment, the astragaloside IV contents reaching the highest content at 3 d Eth treatment(P ≤ 0.05). Moreover, After Eth treatment, all detected key genes involved in astragaloside IV synthesis were significant decrease at 3rd day(P ≤ 0.05). However, SE displayed a significant increase at the 3rd day under Eth treatment(P ≤ 0.05). Under Eth treatment, the expression level of FPS, HMGR, IDI, SS, and CYP93E3 exhibited significant negative correlations with astragaloside IV content, while expression level of SE displayed a significant positive correlation.</p><p><strong>Conclusions: </strong>These findings suggest that exogenous Eth treatment can influence the synthesis of astragaloside IV by regulating the expression of FPS, HMGR, IDI, SS, CYP93E3 and SE. This study provides a theoretical basis for utilizing molecular strategies to enhance the quality of A. membranaceus.</p>","PeriodicalId":9185,"journal":{"name":"Botanical Studies","volume":"65 1","pages":"16"},"PeriodicalIF":3.4,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11226570/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141533581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}