Botanical Studies最新文献

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Morphological characterization of intraspecific variation for trichome traits in tomato (Solanum lycopersicum). 番茄(Solanum lycopersicum)毛状体性状种内变异的形态特征。
IF 3.4 3区 生物学
Botanical Studies Pub Date : 2023-03-29 DOI: 10.1186/s40529-023-00370-3
Satinderpal Kaur, Neetu Khanal, Robert Dearth, Rupesh Kariyat
{"title":"Morphological characterization of intraspecific variation for trichome traits in tomato (Solanum lycopersicum).","authors":"Satinderpal Kaur,&nbsp;Neetu Khanal,&nbsp;Robert Dearth,&nbsp;Rupesh Kariyat","doi":"10.1186/s40529-023-00370-3","DOIUrl":"https://doi.org/10.1186/s40529-023-00370-3","url":null,"abstract":"<p><p>Trichomes, the hairlike protuberances in plants, have been well known to act as the first line of defense against herbivores, and abiotic stresses, along with other structural defenses such as spines, thorns, and waxes. We previously reported the tremendous variation in trichome traits among different wild and cultivated Solanum species and demonstrated that trichomes types and density are traditionally miscalculated and often misnamed. However, intraspecific variation in trichome traits is poorly understood, although this has implications for stress tolerance and resistance breeding programs in economically important crop species and can also mediate ecological interactions at multiple trophic levels in their wild congeners. In this study, using tomato as a model, we characterized the trichomes from 10 commonly grown varieties using a minimal sample prep desktop scanning electron microscopy, and followed up with estimating their dimensions across the varieties and trichome types. We hypothesized that although trichome number may vary, the varieties will have similar trichome types, based on current literature. Our results show that there is significant variation for trichome number as well as dimensions of trichome types among these varieties. Furthermore, when we separately analyzed the number and dimensions of commonly found glandular and non-glandular trichomes, the results were consistent with broad assessment of trichomes, showing consistent variation among varieties, suggesting that trichome studies should not be limited to basic classification into glandular and non-glandular, and should accommodate the sub-types and their dimensions.</p>","PeriodicalId":9185,"journal":{"name":"Botanical Studies","volume":"64 1","pages":"7"},"PeriodicalIF":3.4,"publicationDate":"2023-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10060485/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9592364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Fungal communities on alpine cheese rinds in Southern Switzerland. 瑞士南部高山奶酪皮上的真菌群落。
IF 3.4 3区 生物学
Botanical Studies Pub Date : 2023-03-11 DOI: 10.1186/s40529-023-00371-2
Sophie De Respinis, AnnaPaola Caminada, Elisa Pianta, Antoine Buetti-Dinh, Patrizia Riva Scettrini, Liliane Petrini, Mauro Tonolla, Orlando Petrini
{"title":"Fungal communities on alpine cheese rinds in Southern Switzerland.","authors":"Sophie De Respinis, AnnaPaola Caminada, Elisa Pianta, Antoine Buetti-Dinh, Patrizia Riva Scettrini, Liliane Petrini, Mauro Tonolla, Orlando Petrini","doi":"10.1186/s40529-023-00371-2","DOIUrl":"10.1186/s40529-023-00371-2","url":null,"abstract":"<p><strong>Background: </strong>The biodiversity of the mycobiota of soft cheese rinds such as Brie or Camembert has been extensively studied, but scant information is available on the fungi colonizing the rinds of cheese produced in the Southern Switzerland Alps. This study aimed at exploring the fungal communities present on rinds of cheese matured in five cellars in Southern Switzerland and to evaluate their composition with regards to temperature, relative humidity, type of cheese, as well as microenvironmental and geographic factors. We used macro- and microscopical morphology, matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry, and sequencing to characterize the fungal communities of the cheeses, and compared them with metabarcoding targeting the ITS region.</p><p><strong>Results: </strong>Isolation by serial dilution yielded 201 isolates (39 yeasts and 162 filamentous fungi) belonging to 9 fungal species. Mucor and Penicillium were dominant, with Mucor racemosus, M. lanceolatus, P. biforme, and P. chrysogenum/rubens being the most frequent species. All but two yeast isolates were identified as Debaryomyces hansenii. Metabarcoding detected 80 fungal species. Culture work and metabarcoding produced comparable results in terms of similarity of the fungal cheese rind communities in the five cellars.</p><p><strong>Conclusions: </strong>Our study has shown that the mycobiota on the rinds of the cheeses studied is a comparatively species-poor community influenced by temperature, relative humidity, type of cheese, and manufacturing steps, as well as microenvironmental and possibly geographic factors.</p>","PeriodicalId":9185,"journal":{"name":"Botanical Studies","volume":"64 1","pages":"6"},"PeriodicalIF":3.4,"publicationDate":"2023-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10008522/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9100583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Responses of photosynthesis and chlorophyll fluorescence during light induction in different seedling ages of Mahonia oiwakensis. 光诱导下不同苗龄马洪草光合作用和叶绿素荧光的响应
IF 3.4 3区 生物学
Botanical Studies Pub Date : 2023-03-09 DOI: 10.1186/s40529-023-00369-w
Chung-I Chen, Kuan-Hung Lin, Tzu-Chao Lin, Meng-Yuan Huang, Yung-Chih Chen, Chau-Ching Huang, Ching-Wen Wang
{"title":"Responses of photosynthesis and chlorophyll fluorescence during light induction in different seedling ages of Mahonia oiwakensis.","authors":"Chung-I Chen,&nbsp;Kuan-Hung Lin,&nbsp;Tzu-Chao Lin,&nbsp;Meng-Yuan Huang,&nbsp;Yung-Chih Chen,&nbsp;Chau-Ching Huang,&nbsp;Ching-Wen Wang","doi":"10.1186/s40529-023-00369-w","DOIUrl":"https://doi.org/10.1186/s40529-023-00369-w","url":null,"abstract":"<p><strong>Background: </strong>The aim of this study was to determine the actual state of the photosynthetic apparatus and exhibit distinguishable differences in the chlorophyll fluorescence (ChlF) components in different seedling ages of M. oiwakensis plants subjected to different light intensity (LI). Potted 6-month-old greenhouse seedlings and field collected 2.4-year-old seedlings with 5 cm heights were selected and randomly separated into seven groups for photosynthesis measurements illuminated with 50, 100 (assigned as low LI), 300, 500, 1,000 (as moderate LI), 1,500 and 2,000 (as high LI) μmol m<sup>-2</sup> s<sup>-1</sup> photosynthetic photon flux density (PPFD) treatments.</p><p><strong>Results: </strong>n 6-month-old seedlings, as LI increased from 50 to 2,000 PPFD, the values of non-photochemical quenching and photo-inhibitory quenching (qI) increased but potential quantum efficiency of PSII (Fv/Fm) and photochemical efficiency of photosystem II (ΦPSII) values decreased. High electron transport rate and percentage of actual PSII efficiency by Fv/Fm values were observed in 2.4-year-old seedlings at high LI conditions. Furthermore, higher ΦPSII was detected under low LI conditions, with lower energy-dependent quenching (qE) and qI values and photo-inhibition % decreased as well. However, qE and qI increased as ΦPSII decreased and photo-inhibition% increased under high LI treatments.</p><p><strong>Conclusions: </strong>These results could be useful for predicting the changes in growth and distribution of Mahonia species grown in controlled environments and open fields with various combinations of varying light illuminations, and ecological monitoring of their restoration and habitat creation is important for provenance conservation and helps to formulate better conservation strategies for the seedlings.</p>","PeriodicalId":9185,"journal":{"name":"Botanical Studies","volume":"64 1","pages":"5"},"PeriodicalIF":3.4,"publicationDate":"2023-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9995626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9087038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Alleviation of the adverse effects of NaCl stress on tomato seedlings (Solanum lycopersicum L.) by Trichoderma viride through the antioxidative defense system. 毛霉菌通过抗氧化防御系统缓解氯化钠胁迫对番茄幼苗的不利影响。
IF 3.4 3区 生物学
Botanical Studies Pub Date : 2023-02-09 DOI: 10.1186/s40529-023-00368-x
Rabab A Metwally, Shereen A Soliman
{"title":"Alleviation of the adverse effects of NaCl stress on tomato seedlings (Solanum lycopersicum L.) by Trichoderma viride through the antioxidative defense system.","authors":"Rabab A Metwally, Shereen A Soliman","doi":"10.1186/s40529-023-00368-x","DOIUrl":"10.1186/s40529-023-00368-x","url":null,"abstract":"<p><strong>Background: </strong>Trichoderma viride are well known for their biocontrol capabilities, but little is known about how they stimulate plant development and increase their resistance to salt stress. One of the main abiotic factors limiting crop development and yield is salt stress. Therefore, the purpose of this work was to ascertain how NaCl effects on T. viride growth as well as on the seedlings morphological and physio-biochemical parameters of tomato (Solanum lycopersicum L.) under plate culture conditions. Additionally, a pot experiment was conducted to determine how T. viride affected the development characteristics of tomato plants subjected to various salt concentrations (50 and 100 mM NaCl). T. viride's contribution to tomato seedling stress tolerance was also closely examined.</p><p><strong>Results: </strong>Results showed that 100 mM NaCl decreased the colony diameter of T. viride by 13.4% compared to the control. Under plate and greenhouse conditions, tomato seedlings exposed to salt exposure exhibited an overall decline in growth. Also, a reduction in relative water content (RWC) and protein contents occurred under salt stress. At the same time, increases were found in proline, total phenolics, flavonoids, H<sub>2</sub>O<sub>2</sub> content, malondialdehyde, likewise the activities of peroxidase (POD), catalase (CAT), polyphenol oxidase (PPO), and ascorbate peroxidase (APX) enzymes. Even though, with T. viride application, the salt negative effects on both morphological and physio-biochemical parameters were mitigated to a greater extent. T. viride increased proline and total antioxidant capacity (TAC) in tomato seedlings at 100 mM NaCl by an average of 20.66 and 43.82% compared to their comparable control. T. viride increased the activities of CAT, PPO, and APX enzymes by 74.6, 58.48, and 61.61% at 50 mM NaCl compared to non-saline control seedlings. As well, T. viride decreased MDA and H<sub>2</sub>O<sub>2</sub> contents by an average of 14 and 24.8% in tomato seedlings at 50 mM NaCl compared to their comparable control. Also, under 100 mM NaCl, the T. viride-treated tomato seedlings showed increased total phenolics (17.85%) and flavonoids (33.17%) compared to non- treated one.</p><p><strong>Conclusion: </strong>Hence, our research sheds new insight on the pathways by which T. viride can boost tomato seedling tolerance to salt stress at morphological and physio-biochemical levels by activating both enzymatic and non-enzymatic antioxidant defense systems.</p>","PeriodicalId":9185,"journal":{"name":"Botanical Studies","volume":"64 1","pages":"4"},"PeriodicalIF":3.4,"publicationDate":"2023-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9908811/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10678335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Veronicastrum wulingense (Plantaginaceae), a new species from Southwestern Hubei, China. 标题武林车前草属(车前草科)一新种。
IF 3.4 3区 生物学
Botanical Studies Pub Date : 2023-02-01 DOI: 10.1186/s40529-023-00367-y
Shi-Xiong Ding, Hui Jiang, Jing Tian, Jing Ren, Fredrick Munyao Mutie, Emmanuel Nyongesa Waswa, Guang-Wan Hu, Qing-Feng Wang
{"title":"Veronicastrum wulingense (Plantaginaceae), a new species from Southwestern Hubei, China.","authors":"Shi-Xiong Ding,&nbsp;Hui Jiang,&nbsp;Jing Tian,&nbsp;Jing Ren,&nbsp;Fredrick Munyao Mutie,&nbsp;Emmanuel Nyongesa Waswa,&nbsp;Guang-Wan Hu,&nbsp;Qing-Feng Wang","doi":"10.1186/s40529-023-00367-y","DOIUrl":"https://doi.org/10.1186/s40529-023-00367-y","url":null,"abstract":"<p><strong>Background: </strong>The genus Veronicastrum Heist. ex Fabr. are mainly distributed in East Asia, and only Veronicastrum virginicum (L.) Farw. is disjunctively distributed in eastern North America. The south area of China (extending to Taiwan Island) is the richest in Veronicastrum species. It is of medicinal importance in China as traditional herbs used to treat ascites diseases that caused by schistosomiasis. During field investigation of plant resources in Pingbaying National Forest Park, Southwestern Hubei, China, an unknown flowering population of Veronicastrum was discovered from thick humus layers adjacent to rocks under broad-leaved forests by walkways. They were collected and morphological characters assesed for further taxonomic treatment. Molecular analysis was also conducted to ascertain its phylogenetic position in the genus Veronicastrum.</p><p><strong>Results: </strong>This species is similar to Veronicastrum liukiuense (Ohwi) T.Yamaz. from the Ryukyu Islands, but can be distinctly differed by its axillary inflorescences (versus terminal on short leafy branches), pedicels up to 2.5 mm (versus sessile), corollas purple to purple-red (versus white tinged with pale purple) and florescence June to July (versus September to October). Also, phylogenetic studies showed the species was an independent clade in the genus Veronicastrum based on the maximum likelihood (ML) analyses using two different matrix sequences of concatenated molecular markers. The plastid genome of this new species is also reported in this study for the first time.</p><p><strong>Conclusion: </strong>The morphological and molecular evidences support the recognition of Veronicastrum wulingense as a new species.</p>","PeriodicalId":9185,"journal":{"name":"Botanical Studies","volume":"64 1","pages":"3"},"PeriodicalIF":3.4,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9889593/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10646716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Living on the edge: morphological, karyological and genetic diversity studies of the Hungarian Plantago maxima populations and established ex situ collection. 生活在边缘:匈牙利车前草种群和已建立的迁地收集的形态学、核生物学和遗传多样性研究。
IF 3.4 3区 生物学
Botanical Studies Pub Date : 2023-01-24 DOI: 10.1186/s40529-022-00365-6
Zsófia Kovács, Jelena Mlinarec, Mária Höhn
{"title":"Living on the edge: morphological, karyological and genetic diversity studies of the Hungarian Plantago maxima populations and established ex situ collection.","authors":"Zsófia Kovács,&nbsp;Jelena Mlinarec,&nbsp;Mária Höhn","doi":"10.1186/s40529-022-00365-6","DOIUrl":"https://doi.org/10.1186/s40529-022-00365-6","url":null,"abstract":"<p><strong>Background: </strong>The analysis of genetic diversity of protected plant species can greatly support conservation efforts. Plantago maxima Juss. ex Jacq. is a perennial species distributed along the Eurasian steppe. The westernmost range edge of the species' distribution is located in the Pannonian basin, in Hungary where it is represented by a few, fragmented and highly endangered populations. We studied population diversity of all Hungarian range edge, natural populations, and one established ex situ population. One population from the centre of distribution (Kazakhstan) was implemented in the cpDNA haplotype study to compare the peripheral vs. central populations. We performed morphometric trait-based analysis, chromosome studies (morphometric analyses and FISH) and genetic diversity evaluations using inter simple sequence repeats (ISSR) and cpDNA trnL-trnF to evaluate differences between the in situ and ex situ populations as well as central vs. peripheral populations.</p><p><strong>Results: </strong>Our results showed no obvious morphological differences among the in situ and ex situ populations in the period between 2018 and 2020. One ex situ subpopulation develops flowers three years in a row from 2019, which is a favourable indicator of the introduction success. Hungarian populations are exclusively diploids (2n = 2x = 12). The karyogram consists of 5 metacentric and 1 acrocentric chromosome pair. Plantago maxima has one 35S and two 5S rDNA loci, located on the acrocentric chromosome pair. Eight variable ISSR primers yielded 100 fragments, of which 74.6% were polymorphic (mean H<sub>e</sub> = 0.220). A high level of genetic variation within population was observed (92%) while the genetic differentiation among the populations was only 8%. STRUCTURE analysis revealed that the largest Kunpeszér population separated from the rest of the Hungarian populations, indicating a high rate of admixture among the other ones. Based on the trnL-trnF sequence analysis the Hungarian populations represent a single haplotype, which can indicate a reduced diversity due to isolation and recent population decline. By contrast, Kazakh population represents a distinct haplotype compared to the Hungarian samples.</p><p><strong>Conclusions: </strong>The present study draws the attention to the high conservation value of the Plantago maxima populations from the westernmost range edge of the species' distribution.</p>","PeriodicalId":9185,"journal":{"name":"Botanical Studies","volume":"64 1","pages":"2"},"PeriodicalIF":3.4,"publicationDate":"2023-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9873897/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9176473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
MethylC-analyzer: a comprehensive downstream pipeline for the analysis of genome-wide DNA methylation. MethylC-analyzer:用于全基因组DNA甲基化分析的综合下游管道。
IF 3.4 3区 生物学
Botanical Studies Pub Date : 2023-01-06 DOI: 10.1186/s40529-022-00366-5
Rita Jui-Hsien Lu, Pei-Yu Lin, Ming-Ren Yen, Bing-Heng Wu, Pao-Yang Chen
{"title":"MethylC-analyzer: a comprehensive downstream pipeline for the analysis of genome-wide DNA methylation.","authors":"Rita Jui-Hsien Lu,&nbsp;Pei-Yu Lin,&nbsp;Ming-Ren Yen,&nbsp;Bing-Heng Wu,&nbsp;Pao-Yang Chen","doi":"10.1186/s40529-022-00366-5","DOIUrl":"https://doi.org/10.1186/s40529-022-00366-5","url":null,"abstract":"<p><p>DNA methylation is a crucial epigenetic modification involved in multiple biological processes and diseases. Current approaches for measuring genome-wide DNA methylation via bisulfite sequencing (BS-seq) include whole-genome bisulfite sequencing (WGBS), reduced representation bisulfite sequencing (RRBS), and enzymatic methyl-seq (EM-seq). The computational analysis tools available for BS-seq data include customized aligners for mapping bisulfite-converted reads and computational pipelines for downstream data analysis. Current post-alignment methylation tools are specialized for the interpretation of CG methylation, which is known to dominate mammalian genomes, however, non-CG methylation (CHG and CHH, where H refers to A, C, or T) is commonly observed in plants and fungi and is closely associated with gene regulation, transposon silencing, and plant development. Thus, we have developed a MethylC-analyzer to analyze and visualize post-alignment WGBS, RRBS, and EM-seq data focusing on CG. The tool is able to also analyze non-CG sites to enhance deciphering genomes of plants and fungi. By processing aligned data and gene location files, MethylC-analyzer generates a genome-wide view of methylation levels and methylation in user-specified genomic regions. The meta-plot, for example, allows the investigation of DNA methylation within specific genomic elements. Moreover, our tool identifies differentially methylated regions (DMRs) and investigates the enrichment of genomic features associated with variable methylation. MethylC-analyzer functionality is not limited to specific genomes, and we demonstrated its performance on both plant and human BS-seq data. MethylC-analyzer is a Python- and R-based program designed to perform comprehensive downstream analyses of methylation data, providing an intuitive analysis platform for scientists unfamiliar with DNA methylation analysis. It is available as either a standalone version for command-line uses or a graphical user interface (GUI) and is publicly accessible at https://github.com/RitataLU/MethylC-analyzer .</p>","PeriodicalId":9185,"journal":{"name":"Botanical Studies","volume":"64 1","pages":"1"},"PeriodicalIF":3.4,"publicationDate":"2023-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9823188/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9527595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Biostimulation of tomato growth and biocontrol of Fusarium wilt disease using certain endophytic fungi. 某些内生真菌对番茄生长的生物刺激及枯萎病的生物防治。
IF 3.4 3区 生物学
Botanical Studies Pub Date : 2022-12-09 DOI: 10.1186/s40529-022-00364-7
Amer M Abdelaziz, Mohamed H Kalaba, Amr H Hashem, Mohamed H Sharaf, Mohamed S Attia
{"title":"Biostimulation of tomato growth and biocontrol of Fusarium wilt disease using certain endophytic fungi.","authors":"Amer M Abdelaziz,&nbsp;Mohamed H Kalaba,&nbsp;Amr H Hashem,&nbsp;Mohamed H Sharaf,&nbsp;Mohamed S Attia","doi":"10.1186/s40529-022-00364-7","DOIUrl":"https://doi.org/10.1186/s40529-022-00364-7","url":null,"abstract":"<p><strong>Background: </strong>Tomato plant (Solanum lycopersicum L.) suffers from numerous fungal pathogens that cause damage to yeild production qualitatively and quantitatively. One of the most destructive disease of tomato is Fusarium wilt that caused by soil borne fungus called F. oxysporum.</p><p><strong>Methods: </strong>In this study, the anti-Fusarium capabilities of the foliar application of fungal endophytes extracts have been investigated on tomato under Fusarium challenges. Antifungal assay, inhibition of conidial germination, disease severity, photosynthetic pigments, osmolytes, secondary metabolites, oxidative stress, peroxidase (POD) and polyphenol oxidases (PPO) isozymes were tested for potential resistance of tomato growing under Fusarium infection.</p><p><strong>Results: </strong>Ethyl acetate extracts of A. flavus MZ045563, A. fumigatus MZ045562 and A. nidulans MZ045561 exhibited antifungal activity toward F. oxysporum where inhibition zone diameters were 15, 12 and 20 mm, respectively. Moreover, extracts of all fungal isolates at concentration 7.5 mg/mL reduced conidia germination from 94.4 to 100%. Fusarium infection caused a destructive effects on tomato plant, high severity desiese index 84.37%, reduction in growth parameters, photosynthetic pigments, and soluble protein. However, contents of proline, total phenol, malondialdehyde (MDA), hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and antioxidant enzymes activity were increased in tomato plants grown under Fusarium wilt. Treatment of healthy or infected tomato plants by ethyl acetate fungal extracts showed improvements in morphological traits, photosynthetic pigments, osmolytes, total phenol and antioxidant enzymes activity. Besides, the harmful impacts of Fusarium wilt disease on tomato plants have also been reduced by lowering MDA and H<sub>2</sub>O<sub>2</sub> levels. Also, treated tomato plants showed different responses in number and density of POD and PPO isozymes.</p><p><strong>Conclusion: </strong>It could be suggested that application of ethyl acetate extracts of tested fungal endophytes especially combination of A. flavus, A. nidulans and A. fumigatus could be commercially used as safe biostimulation of tomato plants as well as biofungicide against tomato Fusarium wilt disease.</p>","PeriodicalId":9185,"journal":{"name":"Botanical Studies","volume":"63 1","pages":"34"},"PeriodicalIF":3.4,"publicationDate":"2022-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9733755/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10378533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
First report of chemical composition and cytotoxicity evaluation of Foraminispora rugosa basidiomata from Brazil. 巴西有孔孢子虫担子瘤化学成分及细胞毒性评价首次报道。
IF 3.4 3区 生物学
Botanical Studies Pub Date : 2022-11-26 DOI: 10.1186/s40529-022-00363-8
V O Garcia, M Fronza, R Von Borowski, G Alves-Silva, A R Zimmer, T Ruaro, S C B Gnoatto, A Dallegrave, R M B Silveira
{"title":"First report of chemical composition and cytotoxicity evaluation of Foraminispora rugosa basidiomata from Brazil.","authors":"V O Garcia,&nbsp;M Fronza,&nbsp;R Von Borowski,&nbsp;G Alves-Silva,&nbsp;A R Zimmer,&nbsp;T Ruaro,&nbsp;S C B Gnoatto,&nbsp;A Dallegrave,&nbsp;R M B Silveira","doi":"10.1186/s40529-022-00363-8","DOIUrl":"https://doi.org/10.1186/s40529-022-00363-8","url":null,"abstract":"<p><strong>Background: </strong>Foraminispora rugosa is a species reported from Brazil, Venezuela, French Guiana, Costa Rica and Cuba. It is a basidiomycete in the Ganodermataceae family. In this study, both chemical composition and cytotoxicity of the ethanolic extract of F. rugosa were investigated for the first time.</p><p><strong>Results: </strong>Phylogenetic analysis confirmed the identification of the specimens, and the results of cytotoxicity assays showed that at concentrations of 7.8-500.0 µg/mL the ethanolic extract displayed weak cytotoxicity against the tested cell lines. Five oxylipins were identified by ultra high performance liquid chromatography coupled with quadrupole time-of-flight and mass spectrometry (UHPLC-QTOF-MS).</p><p><strong>Conclusions: </strong>This study provides new insights into the current knowledge of bioactive compounds produced by macrofungi, and provides data for future biological assays with relative selectivity and safety.</p>","PeriodicalId":9185,"journal":{"name":"Botanical Studies","volume":" ","pages":"33"},"PeriodicalIF":3.4,"publicationDate":"2022-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9701286/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40706457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction: Southern Carpathian ultramafic grasslands within the central-southeast European context: syntaxonomic classification and overall eco-coenotic patterns. 更正:中欧-东南欧喀尔巴阡山脉南部的超镁铁质草原:分类学分类和整体生态格局。
IF 3.4 3区 生物学
Botanical Studies Pub Date : 2022-11-21 DOI: 10.1186/s40529-022-00362-9
Gheorghe Coldea, Dan Gafta, Gavril Negrean, Adrian Ilie Stoica, Bogdan-Iuliu Hurdu
{"title":"Correction: Southern Carpathian ultramafic grasslands within the central-southeast European context: syntaxonomic classification and overall eco-coenotic patterns.","authors":"Gheorghe Coldea,&nbsp;Dan Gafta,&nbsp;Gavril Negrean,&nbsp;Adrian Ilie Stoica,&nbsp;Bogdan-Iuliu Hurdu","doi":"10.1186/s40529-022-00362-9","DOIUrl":"https://doi.org/10.1186/s40529-022-00362-9","url":null,"abstract":"","PeriodicalId":9185,"journal":{"name":"Botanical Studies","volume":" ","pages":"32"},"PeriodicalIF":3.4,"publicationDate":"2022-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9679104/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40718354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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