Botanical Studies最新文献

{"title":"Analysing protein complexes in plant science: insights and limitation with AlphaFold 3.","authors":"Pei-Yu Lin, Shiang-Chin Huang, Kuan-Lin Chen, Yu-Chun Huang, Chia-Yu Liao, Guan-Jun Lin, HueyTyng Lee, Pao-Yang Chen","doi":"10.1186/s40529-025-00462-2","DOIUrl":"10.1186/s40529-025-00462-2","url":null,"abstract":"<p><p>AlphaFold 3 (AF3), an artificial intelligence (AI)-based software for protein complex structure prediction, represents a significant advancement in structural biology. Its flexibility and enhanced scalability have unlocked new applications in various fields, specifically in plant science, including improving crop resilience and predicting the structures of plant-specific proteins involved in stress responses, signalling pathways, and immune responses. Comparisons with existing tools, such as ClusPro and AlphaPulldown, highlight AF3's unique strengths in sequence-based interaction predictions and its greater adaptability to various biomolecular structures. However, limitations persist, including challenges in modelling large complexes, protein dynamics, and structures from underrepresented plant proteins with limited evolutionary data. Additionally, AF3 encounters difficulties in predicting mutation effects on protein interactions and DNA binding, which can be improved with molecular dynamics and experimental validation. This review presents an overview of AF3's advancements, using examples in plant and fungal research, and comparisons with existing tools. It also discusses current limitations and offers perspectives on integrating molecular dynamics and experimental validation to enhance its capabilities.</p>","PeriodicalId":9185,"journal":{"name":"Botanical Studies","volume":"66 1","pages":"14"},"PeriodicalIF":3.4,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12098255/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative metabolomics of acetylcholinesterase and α-glucosidase inhibitors in pericarp of Garcinia mangostana L.","authors":"Yun-Han Wang, Ta-Wei Liu, Sui-Wen Hsiao, Man-Hsiu Chu, Tzong-Huei Lee, Su-Jung Hsu, Shih Yin Chen, Ching-Kuo Lee","doi":"10.1186/s40529-025-00460-4","DOIUrl":"10.1186/s40529-025-00460-4","url":null,"abstract":"<p><strong>Background: </strong>Mangosteen (Garcinia mangostana L.) pericarp extract has demonstrated potential against Alzheimer's disease (AD) and diabetes mellitus (DM). This study introduces a rapid dereplication and comparative approach to identify and characterize acetylcholinesterase (AChE) and α-glucosidase inhibitors in mangosteen pericarp. Using protein-subtraction, MS profiling, and computational modeling is effective for screening, identifying, and analyzing enzyme-inhibiting compounds from plant sources, and quantitative analysis of the main components has been performed.</p><p><strong>Results: </strong>The Mangosteen pericarp extract observed significant inhibitory activity against α-glucosidase and AChE, with IC50 values of 31.02 and 70.56 µg/mL, respectively. By comparing profiles of protein-subtracted extracts with non-treated extracts, eight potential inhibitors for each enzyme were identified: 8-desoxygartanin, gartanin, 3-isomangostin, β-mangostin, 9-hydroxycalabaxanthone, γ-mangostin, α-mangostin, and garcinone E. The α-mangostin was the most abundant, comprising 39.589% of the extract. Molecular docking revealed these inhibitors target the peripheral anionic site of AChE and the active site of α-glucosidase, forming key hydrogen bonds and pi-pi stacking interactions.</p><p><strong>Conclusion: </strong>This study emphasizes mangosteen pericarp as a promising natural source of these inhibitors, with potential for use in developing nutraceuticals and pharmaceuticals. The study validated a systems biology approach by applying dereplication and comparative UPLC-ESI-MS/MS metabolomics profiling to identify target-binding molecules in both protein-treated and untreated plant extracts. Further confirmation was obtained through molecular docking predictions, mechanism analysis, and compound quantification assays.</p>","PeriodicalId":9185,"journal":{"name":"Botanical Studies","volume":"66 1","pages":"13"},"PeriodicalIF":3.4,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12095715/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Chromosome number variation and phylogenetic divergence of East Asian Cirsium sect. Onotrophe subsect. Nipponocirsium (Compositae), with a new species from Taiwan.","authors":"Chih-Yi Chang, Pei-Chun Liao, Hsy-Yu Tzeng, Junko Kusumi, Zhi-Hui Su, Yen-Hsueh Tseng","doi":"10.1186/s40529-025-00458-y","DOIUrl":"10.1186/s40529-025-00458-y","url":null,"abstract":"","PeriodicalId":9185,"journal":{"name":"Botanical Studies","volume":"66 1","pages":"12"},"PeriodicalIF":3.4,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950611/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leaf dry mass per unit area and leaf pigments underlying the higher stomatal conductance of deciduous species relative to evergreen species in Dendrobium.","authors":"Feng-Ping Zhang, Xiao-Di Zhao, Li-Jun Han, Han-Run Li","doi":"10.1186/s40529-025-00457-z","DOIUrl":"10.1186/s40529-025-00457-z","url":null,"abstract":"<p><strong>Background: </strong>Leaf stomatal conductance is an important indicator of photosynthetic capacity. However, stomatal conductance is poorly quantified and rarely explored in the context of the leaf functional traits for epiphytes, particularly when it comes to herbaceous species with different leaf habits (deciduous vs. deciduous species). Here, we investigated leaf stomatal conductance, leaf dry mass per unit area, leaf thickness, stomatal density, abaxial epidermal cell size and pigment contents in 23 Dendrobium evergreen and deciduous species from a greenhouse. Our main objectives were to compare differences in all measured traits between evergreen and deciduous species, and to determine the relationships of leaf stomatal conductance with leaf functional traits and leaf pigments.</p><p><strong>Results: </strong>The results showed that the evergreen species of Dendrobium had thicker leaves and higher leaf dry mass per unit area, whereas deciduous species had higher leaf stomatal conductance and higher leaf chlorophyll contents. Leaf stomatal conductance had a negative correlation with leaf thickness, and dry mass per unit area, but a positive correlation with leaf pigment contents. There was a negative correlation between pigment contents and leaf dry mass per unit area.</p><p><strong>Conclusion: </strong>The results reveal the clear differences in leaf stomatal conductance, leaf functional traits and leaf pigments between deciduous and evergreen Dendrobium species, with the form groups showing trait values indicative of less investments in structural components and of more investments in photosynthetic carbon gain. Furthermore, leaf dry mass per unit area and leaf pigments play an important role in shaping leaf stomatal conductance.</p>","PeriodicalId":9185,"journal":{"name":"Botanical Studies","volume":"66 1","pages":"11"},"PeriodicalIF":3.4,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11930909/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143691029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biogeographic patterns and evolutionary history of Elatostema (Urticaceae).","authors":"Yu-Hsin Tseng, Alex K Monro, Jer-Ming Hu","doi":"10.1186/s40529-025-00456-0","DOIUrl":"10.1186/s40529-025-00456-0","url":null,"abstract":"<p><strong>Background: </strong>The paleotropics, home to half of Earth's rainforests, exhibit remarkable biodiversity and complex biogeographic patterns. Understanding the intercontinental distribution of plant taxa between Africa and Asia in this region is crucial for resolving longstanding debates on plant evolution and dispersal mechanisms. This study investigates the genus Elatostema, a widely distributed taxon found in subtropical and tropical Africa, Asia, and Australasia, aiming to elucidate the factors shaping its modern tropical disjunctions and evolutionary history.</p><p><strong>Result: </strong>Using molecular dating and ancestral area reconstruction, we reconstructed the historical biogeographic pattern of Elatostema. Our results indicated that the genus originated in tropical Asia during the Eocene, likely associated with boreotropical floras. The evolutionary history of Elatostema involved multiple intercontinental dispersal events, including two independent colonizations of Africa from Asia. Diversification within the core Elatostema clade was primarily driven by events in Asia and Oceania, with key factors contributing to this diversification including reciprocal dispersal between Malesia and Australasia, eastward island hopping and karstification in China. Furthermore, a geographical phylogenetic structure was observed within the core Elatostema clade, possibly due to limited seed and pollen dispersal.</p><p><strong>Conclusions: </strong>The present study provides the first comprehensive insights into the biogeography and evolution of Elatostema. The presence of numerous narrowly distributed endemics, relatively few widespread species, and geographical structures within Elatostema suggest that limited gene flow may be a crucial factor in speciation and evolutionary processes, similar to other species-rich genera.</p>","PeriodicalId":9185,"journal":{"name":"Botanical Studies","volume":"66 1","pages":"10"},"PeriodicalIF":3.4,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11923331/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of arbuscular mycorrhizal fungi in drought-resilient soybeans (Glycine max L.): unraveling the morphological, physio-biochemical traits, and expression of polyamine biosynthesis genes.","authors":"Elham R S Soliman, Reda E Abdelhameed, Rabab A Metwally","doi":"10.1186/s40529-025-00455-1","DOIUrl":"10.1186/s40529-025-00455-1","url":null,"abstract":"<p><strong>Background: </strong>Drought stress is a catastrophic abiotic stressor that impedes the worldwide output of commodities and the development of plants. The Utilizing biological antioxidant stimulators, Arbuscular mycorrhizal fungi (AMF) are one example increased the plants' ability to withstand the effects of drought. The symbiotic response of soybean (Glycine max L.) to AMF inoculation was assessed in the experiment presented herewith at different watering regimes (field capacity of 25, 50, and 90%). The vegetative, physio-biochemical traits, and regulation of genes involved in polyamine synthesis in G. max plants were evaluated.</p><p><strong>Results: </strong>The results obtained suggested that AMF inoculation has an advantage over plants that were non-inoculated in terms of their growth and all assessed criteria, which responded to drought stress by showing slower development. It is evident that the gas exchange parameters of the soybean plant were substantially reduced by 36.79 (photosynthetic rate; A), 60.59 (transpiration rate; E), and 53.50% (stomatal conductance gs), respectively, under severe stress of drought in comparison to control; non-stressed treatment. However, the AMF inoculation resulted in a 40.87, 29.89, and 33.65% increase in A, E, and gs levels, respectively, in extremely drought-stressful circumstances, when in contrast to non-AMF one that was grown under well-watered conditions. The drought level was inversely proportional to mycorrhizal colonization. The total antioxidant capacity, protein, and proline contents were all enhanced by AMF inoculation, while the malondialdehyde and hydrogen peroxide contents were decreased. Polyamine biosynthesis genes expression; Ornithine decarboxylase (ODC2), Spermidine synthase (SPDS) and Spermine synthase (SpS) were upregulated in drought and to even higher level in AMF's mild drought inoculated plants' shoots. This implies that AMF plays apart in the enhanced survival of soybean plants stressed by drought and reduced plant membranes damage by limiting the excessive production of oxidative stress generators; ROS.</p><p><strong>Conclusions: </strong>In summary, the present investigation demonstrates that inoculation of AMF may be a supportable and environmentally advantageous method for improving the physio-biochemical traits, plant growth, and polyamine biosynthesis genes of soybean plants in the incident of limited water availability.</p>","PeriodicalId":9185,"journal":{"name":"Botanical Studies","volume":"66 1","pages":"9"},"PeriodicalIF":3.4,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11914442/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143647123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chromosome number variation and phylogenetic divergence of East Asian Cirsium sect. Onotrophe subsect. Nipponocirsium (Compositae), with a new species from Taiwan.","authors":"Chih-Yi Chang, Pei-Chun Liao, Hsy-Yu Tzeng, Junko Kusumi, Zhi-Hui Su, Yen-Hsueh Tseng","doi":"10.1186/s40529-025-00454-2","DOIUrl":"10.1186/s40529-025-00454-2","url":null,"abstract":"<p><strong>Background: </strong>This study explored chromosome number variation, phylogenetic divergence, and mechanisms underlying speciation in East Asian thistle Cirsium Mill. sect. Onotrophe (Cass.) DC. subsect. Nipponocirsium Kitam. (Compositae). The study focused on the newly identified species from Taiwan: Cirsium pengii Y.H. Tseng, P.C. Liao & Chih Y. Chang. Utilizing phylotranscriptomic data to reconstruct evolutionary relationships between the Taiwanese and Japanese taxa of Cirsium subsect. Nipponocirsium as well as their divergence times and chromosomal characteristics. Additionally, the chromosome number, morphology, and pollen morphology of the unknown Cirsium taxon are compared with other known subsect. Nipponocirsium taxa from Taiwan.</p><p><strong>Results: </strong>Phylotranscriptomic analysis reveals a division within subsect. Nipponocirsium into Japanese and Taiwanese clades. In the Taiwanese clade, C. pengii is basal, while C. tatakaense remains monophyletic with other Taiwanese species despite higher genetic diversity. The prevalent chromosome number in this subsection is tetraploid (2n = 4x = 68), common in Japanese taxa, while Taiwanese members have 2n = 4x = 64. Notably, C. pengii has a diploid number (2n = 32), indicating descending dysploidy followed by polyploidization in Taiwan. This polyploidization, driven by glaciations, likely shaped the evolution of Nipponocirsium. Divergence time estimates suggest the separation of Japanese and Taiwanese clades around 0.74 million years ago (Myr) during glacial periods. Cirsium pengii diverged around 0.47 Myr, while tetraploid species C. kawakamii and C. tatakaense diverged around 0.35 Myr. These species likely evolved in separate refugia, with distinct species boundaries confirmed through species delimitation analysis, karyotype, morphology, and pollen morphology comparisons.</p><p><strong>Conclusions: </strong>These findings enhance our understanding of chromosomal evolution and speciation within subsect. Nipponocirsium and underscore the importance of integrating transcriptomic data in phylogenetic studies. This study provides a comprehensive framework for further investigations into the genetic diversity and adaptive mechanisms of this ecologically vital group.</p>","PeriodicalId":9185,"journal":{"name":"Botanical Studies","volume":"66 1","pages":"8"},"PeriodicalIF":3.4,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11828781/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143424952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A plant endophytic bacterium Burkholderia seminalis strain 869T2 increases plant growth under salt stress by affecting several phytohormone response pathways.","authors":"Hau-Hsuan Hwang, Yu-Ting Huang, Pei-Ru Chien, Fan-Chen Huang, Chih-Lin Wu, Liang-Yu Chen, Shih-Hsun Walter Hung, I-Chun Pan, Chieh-Chen Huang","doi":"10.1186/s40529-025-00453-3","DOIUrl":"10.1186/s40529-025-00453-3","url":null,"abstract":"<p><strong>Background: </strong>Due to global warming and gradual climate change, plants are subjected to a wide range of environmental stresses, adversely affecting plant growth and production worldwide. Plants have developed various mechanisms to overpower these abiotic stresses, including salt stress, drought, and high light intensity. Apart from their own defense strategies, plants can get help from the beneficial endophytic bacteria inside host plants and assist them in enduring severe growth conditions. A previously isolated plant endophytic bacteria, Burkholderia seminalis 869T2, from vetiver grass can produce auxin, synthesize siderophore, and solubilize phosphate. The B. seminalis 869T2 can colonize inside host plants and increase the growth of bananas, Arabidopsis, and several leafy vegetables.</p><p><strong>Results: </strong>We further demonstrated that different growth parameters of Arabidopsis and pak choi plants were significantly increased after inoculating the B. seminalis 869T2 under normal, salt, and drought stress conditions compared to the mock-inoculated plants. Both transcriptome analysis and quantitative real-time PCR results showed that expression levels of genes related to phytohormone signal transduction pathways, including auxin, gibberellin, cytokinin, and abscisic acid were altered in Arabidopsis plants after inoculated with the strain 869T2 under salt stress, in comparison to the mock-inoculated control with salt treatments. Furthermore, the accumulation levels of hydrogen peroxide (H₂O₂), electrolyte leakage (EL), and malondialdehyde (MDA) were lower in the 869T2-inoculated Arabidopsis and pak choi plants than in control plants under salt and drought stresses.</p><p><strong>Conclusions: </strong>The plant endophytic bacterium strain B. seminalis 869T2 may affect various phytohormone responses and reduce oxidative stress damage to increase salt and drought stress tolerances of host plants.</p>","PeriodicalId":9185,"journal":{"name":"Botanical Studies","volume":"66 1","pages":"7"},"PeriodicalIF":3.4,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11794907/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143188382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing water status in rice plants in water-deficient environments using thermal imaging.","authors":"Chin-Ying Yang, Yan-Ci Zhang, Ya-Ling Hou","doi":"10.1186/s40529-025-00452-4","DOIUrl":"10.1186/s40529-025-00452-4","url":null,"abstract":"<p><strong>Background: </strong>Rice is a staple food for the global population. However, extreme weather events threaten the stability of the water supply for agriculture, posing a critical challenge to the stability of the food supply. The use of technology to assess the water status of rice plants enables the precise management of agricultural water resources.</p><p><strong>Results: </strong>The results of this study reveal that rice-producing regions with more severe drought have higher ion leakage rates, lower Soil Plant Analysis Development (SPAD) meter values, and reduced total chlorophyll content in plants. Although no significant differences were observed in red-green-blue (RGB) images, physiological parameters and canopy temperature differed significantly from conventional farming when infrared thermography was used to capture rice plants in the early stages of drought. The Crop Water Stress Index (CWSI), calculated from canopy temperature and atmospheric temperature, indicated a high correlation between access to water for rice plants and their physiological parameters. Regression analysis using CWSI and plant water status yielded a corrected coefficient of determination of 0.86.</p><p><strong>Conclusion: </strong>Our study demonstrate that infrared thermography can effectively detect early signs of water stress in rice, aiding farmers in irrigation planning and enabling precise management and optimal utilization of water resources.</p>","PeriodicalId":9185,"journal":{"name":"Botanical Studies","volume":"66 1","pages":"6"},"PeriodicalIF":3.4,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11769923/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic and enzymatic analysis of peroxidase families at the early growth stage of halophyte ice plant (Mesembryanthemum crystallinum L.) under salt stress.","authors":"Li-Ching Hsieh, Chia-Che Lee, Kai-Fu Zhang, Hui-Hsien Chang, Cheng-Hsun Li, Hsuan-Jung Huang, Hungchen Emilie Yen","doi":"10.1186/s40529-024-00450-y","DOIUrl":"10.1186/s40529-024-00450-y","url":null,"abstract":"<p><p>Ice plant (Mesembryanthemum crystallinum L.) is a halophyte and an inducible CAM plant. Ice plant seedlings display moderate salt tolerance, with root growth unaffected by 200 mM NaCl treatments, though hypocotyl elongation is hindered in salt-stressed etiolated seedlings. Superoxide anion accumulation was prominent in cotyledons and primary leaves but decreased in root tissues over time, with no significant effect from salt treatment. Hydrogen peroxide levels initially surged in both control and salt-treated seedlings, with higher and more persistent accumulation in the salt-treated seedlings. The activities of H₂O₂-scavenging ascorbate-glutathione cycle enzymes ascorbate peroxidase (APX), monodehydroascorbate reductase, and dehydroascorbate reductase increased, while guaiacol-dependent peroxidase activity decreased and catalase activity showed no change, indicating APX activity as the primary response to salt stress. Salt-induced APX activities were detected mainly in the microsomal fraction for light-grown seedlings and the cytosolic fraction for etiolated seedlings, highlighting plastids as the primary site of ROS accumulation under salt stress. An RNA-seq analysis of etiolated seedlings revealed about 8% unigenes showing more than a four-fold change in expression after a 6-h 200 mM NaCl treatment. GO enrichment analysis indicated that differentially expressed genes (DEGs) with increased transcript abundance were associated with ion transport, antioxidant activity, and stress responses, while DEGs with decreased transcript abundance were linked to metabolic and biosynthesis processes such as ribosomal protein synthesis and cell wall formation. This indicates that salt stress hinders growth but enhances ion homeostasis and stress response mechanisms. The expression of all eight APX genes were induced by a 48-hour salt treatment, with varying expression patterns. For class III peroxidase family, 14 out of 53 identified unigenes qualified as DEGs. The time-course expression patterns revealed that the transcript levels of McPrx4.1, McPrx12.1, and McPrx12.3 increased, while McPrx60.3 decreased. These findings highlight the distinct roles of class III peroxidases in balancing plant growth and stress responses, advancing our understanding of the mechanisms behind salt tolerance in halophytes. This study comprehensively analyzed changes in gene expression, antioxidant enzyme activity, and ROS accumulation in ice plant seedlings. Unveiling these responses will advance our understanding of the growth-stress balance in the intrinsic salt tolerance in halophytes.</p>","PeriodicalId":9185,"journal":{"name":"Botanical Studies","volume":"66 1","pages":"5"},"PeriodicalIF":3.4,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11751343/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143000278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}