Transcriptome profiles reveal gene regulation of ginger flowering induced by photoperiod and light quality.

IF 3.4 3区 生物学 Q1 Agricultural and Biological Sciences
Qinyu Deng, Yangtao Zhang, Kang Liu, Guo Zheng, Longyan Gao, Zhexin Li, Mengjun Huang, Yusong Jiang
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引用次数: 0

Abstract

Background: Under natural conditions, ginger (Zingiber officinale Rosc.) rarely blossom and has seed, which limits new variety breeding of ginger and industry development. In this study, the effects of different photoperiods and light quality on flowering induction in ginger were performed, followed by gene expression analysis of flower buds differentiation under induced treatment using RNA-seq technology.

Results: First, both red light and long light condition (18 h light/6 h dark) could effectively induce differentiation of flower buds in ginger. Second, a total of 3395 differentially expressed genes were identified from several different comparisons, among which nine genes, including CDF1, COP1, GHD7, RAV2-like, CO, FT, SOC1, AP1 and LFY, were identified to be associated with flowering in induced flower buds and natural leaf buds. Aside from four down-regulated genes (CDF1, COP1, GHD7 and RAV2-like), other five genes were all up-regulated expression. These differentially expressed genes were mainly classified into 2604 GO categories, which were further enriched into 120 KEGG metabolic pathways. Third, expression change of flowering-related genes in ginger indicated that the induction may negatively regulated expression of CDF1, COP1, GHD7 and RAV2-like, and subsequently positively regulated expression of CO, FT, SOC1, LFY and AP1, which finally led to ginger flowering. In addition, the RNA-seq results were verified by qRT-PCR analysis of 18 randomly selected genes, which further demonstrated the reliability of transcriptome analysis.

Conclusion: This study revealed the ginger flowering mechanism induced by light treatment and provided abundant gene information, which contribute to the development of hybrid breeding of ginger.

Abstract Image

Abstract Image

Abstract Image

转录组分析揭示了光周期和光质量诱导姜开花的基因调控。
背景:在自然条件下,生姜(Zingiber officinale Rosc.)很少开花且有种子,这限制了生姜新品种的选育和产业发展。本研究研究了不同光周期和光质量对生姜开花诱导的影响,并利用RNA-seq技术分析了诱导处理下生姜花芽分化的基因表达。结果:①红光和长光照条件(光照18 h /暗6 h)均能有效诱导生姜花芽分化;其次,从多个不同的比较中共鉴定出3395个差异表达基因,其中CDF1、COP1、GHD7、RAV2-like、CO、FT、SOC1、AP1和LFY等9个基因在诱导花蕾和天然叶蕾中与开花相关。除CDF1、COP1、GHD7、RAV2-like 4个基因表达下调外,其余5个基因均表达上调。这些差异表达基因主要分为2604个GO类,并进一步富集为120个KEGG代谢途径。第三,生姜开花相关基因的表达变化表明,诱导可以负调控CDF1、COP1、GHD7和rav2样基因的表达,随后正调控CO、FT、SOC1、LFY和AP1的表达,最终导致生姜开花。另外,对随机选取的18个基因进行qRT-PCR分析,验证了RNA-seq结果,进一步证明了转录组分析的可靠性。结论:本研究揭示了光处理诱导生姜开花的机制,提供了丰富的基因信息,为生姜杂交育种的开展提供了依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Botanical Studies
Botanical Studies 生物-植物科学
CiteScore
5.50
自引率
2.90%
发文量
32
审稿时长
2.4 months
期刊介绍: Botanical Studies is an open access journal that encompasses all aspects of botany, including but not limited to taxonomy, morphology, development, genetics, evolution, reproduction, systematics, and biodiversity of all plant groups, algae, and fungi. The journal is affiliated with the Institute of Plant and Microbial Biology, Academia Sinica, Taiwan.
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