Anther-derived microspore embryogenesis in pepper hybrids orobelle and Bomby.

IF 3.4 3区 生物学 Q1 Agricultural and Biological Sciences
K P Sahana, Arpita Srivastava, Anil Khar, Neelu Jain, P K Jain, Hemlata Bharti, Mohd Harun, Manisha Mangal
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引用次数: 0

Abstract

Background: Traditional breeding methods have long been employed worldwide for the evaluation and development of pepper cultivars. However, these methods necessitate multiple generations of screening, line development, evaluation, recognition, and crossing to obtain highly homozygous lines. In contrast, in vitro anther-derived microspore culture represents a rapid method to generate homozygous lines within a single generation. In the present study, we have optimized a protocol for microspore embryogenesis from anther cultures of pepper hybrids Orobelle and Bomby.

Results: We achieved early and successful embryo formation from both genotypes by subjecting the buds to a cold pretreatment at 4 °C for 4 days. Our optimized culture medium, comprised of MS medium supplemented with 4 mg/L NAA, 1 mg/L BAP, 0.25% activated charcoal, 2.6 g/L gelrite, 30 g/L sucrose, and 15 mg/L silver nitrate, exhibited the highest efficiency in embryo formation (1.85% and 1.46%) for Orobelle and Bomby, respectively. Furthermore, successful plant regeneration from the anther derived microspore embryos was accomplished using half-strength MS medium fortified with 2% sucrose and 0.1 mg/L 6-benzylaminopurine (BA), solidified with 2.6 g/L gelrite. The ploidy status of the microspore-derived plantlets was analyzed using flow cytometry technique. Notably, the haploid plants exhibited distinct characteristics such as reduced plant height, leaf length, leaf width, and shorter internode length when compared to their diploid counterparts derived from seeds.

Conclusion: Our findings highlight the potential of anther culture and microspore embryogenesis as an advanced method for accelerating pepper breeding programs, enabling the rapid production of superior homozygous lines.

辣椒杂交种 orobelle 和 Bomby 的花药小孢子胚胎发生。
背景:长期以来,全世界一直采用传统育种方法来评估和培育辣椒栽培品种。然而,这些方法需要经过多代筛选、品系开发、评估、识别和杂交才能获得高同质性品系。相比之下,体外花药小孢子培养是一种快速方法,可在一代内产生同源品系。在本研究中,我们对辣椒杂交种 Orobelle 和 Bomby 的花药小孢子胚胎发生方案进行了优化:结果:通过将花蕾在 4 °C 下进行 4 天的低温预处理,我们实现了这两种基因型胚胎的早期成功形成。我们优化的培养基由 MS 培养基组成,其中添加了 4 毫克/升 NAA、1 毫克/升 BAP、0.25% 活性炭、2.6 克/升明胶、30 克/升蔗糖和 15 毫克/升硝酸银,结果显示,Orobelle 和 Bomby 的胚胎形成效率最高(分别为 1.85% 和 1.46%)。此外,使用添加了 2% 蔗糖和 0.1 mg/L 6-苄基氨基嘌呤(BA)的半强度 MS 培养基,并用 2.6 g/L 凝胶凝固,也能成功地从花药衍生的小孢子胚中获得植株再生。利用流式细胞仪技术分析了小孢子衍生小植株的倍性状况。值得注意的是,与种子衍生的二倍体植株相比,单倍体植株表现出明显的特征,如株高、叶长、叶宽降低,节间长度变短:我们的研究结果凸显了花药培养和小孢子胚胎发生作为加速辣椒育种计划的先进方法的潜力,可快速培育出优良的同源品系。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Botanical Studies
Botanical Studies 生物-植物科学
CiteScore
5.50
自引率
2.90%
发文量
32
审稿时长
2.4 months
期刊介绍: Botanical Studies is an open access journal that encompasses all aspects of botany, including but not limited to taxonomy, morphology, development, genetics, evolution, reproduction, systematics, and biodiversity of all plant groups, algae, and fungi. The journal is affiliated with the Institute of Plant and Microbial Biology, Academia Sinica, Taiwan.
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