Biomarkers in medicine最新文献

{"title":"Association between miRNA polymorphisms and rheumatoid arthritis susceptibility: a meta-analysis.","authors":"Young Ho Lee, Gwan Gyu Song","doi":"10.1080/17520363.2025.2577626","DOIUrl":"10.1080/17520363.2025.2577626","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the association between microRNA (miRNA) polymorphisms and rheumatoid arthritis (RA) susceptibility through meta-analysis.</p><p><strong>Methods: </strong>We systematically searched MEDLINE, EMBASE, and Web of Science for case-control studies examining five miRNA polymorphisms: miR-146a rs2910164, miR-499 rs3746444, miR-196a2 rs11614913, miR-155 rs767649, and miR-149 rs2292832. Odds ratios (ORs) with 95% confidence intervals (CIs) were calculated using random-effects models.</p><p><strong>Results: </strong>Twenty-three studies comprising 4,303 RA patients and 5,337 controls were included. Significant associations were identified for three polymorphisms. MiR-499 rs3746444 demonstrated increased RA risk across multiple genetic models (allelic, recessive, and homozygote). MiR-146a rs2910164 showed population-specific effects, conferring protection in Arabs (OR = 0.666, 95% CI = 0.520-0.853, <i>p</i> = 0.001) but not in Asian or European populations. The miR-149 rs2292832 A allele exhibited strong protective effects (OR = 0.314, 95% CI = 0.215-0.458, <i>p</i> < 0.001). No significant associations were observed for miR-196a2 rs11614913 or miR-155 rs767649 polymorphisms.</p><p><strong>Conclusions: </strong>This meta-analysis demonstrates that miRNA polymorphisms exhibit variable associations with RA susceptibility. MiR-499 rs3746444 increases risk, while miR-146a rs2910164 and miR-149 rs2292832 provide protection, with ethnic-specific effects for miR-146a rs2910164.</p>","PeriodicalId":9182,"journal":{"name":"Biomarkers in medicine","volume":" ","pages":"947-955"},"PeriodicalIF":2.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12622312/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145336386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic significance of combined serum immunological HER2 and ERα36 biomarkers in estrogen receptor-positive breast cancer subtypes.","authors":"Yiyang Guo, Shiru Cao, Yanli Deng, Hong Zhang","doi":"10.1080/17520363.2025.2577630","DOIUrl":"10.1080/17520363.2025.2577630","url":null,"abstract":"<p><strong>Background: </strong>Breast cancer is the most common cancer among women, with rising incidence and mortality rates, posing clinical treatment challenges.</p><p><strong>Objective: </strong>To analyze the prognostic significance of serum immune HER2 and ERα36 biomarkers in estrogen receptor-positive breast cancer subtypes.</p><p><strong>Methods: </strong>Sixty-one estrogen receptor-positive breast cancer cases from Fourth Hospital of Hebei Medical University (2016-2023) were analyzed. The qPCR was used to detect the expression level of ERα36 mRNA in the sample, and ELISA was used to detect the expression of HER2 in the sample. Associations between biomarker expression, clinicopathological features, and prognosis were evaluated.</p><p><strong>Results: </strong>ERα36 mRNA expression was higher in breast cancer tissues than in adjacent tissues (<i>p</i> < 0.05). High ERα36 expression was associated with menopause (77.78%) and lymph node metastasis (75.00%) (<i>p</i> < 0.05). HER2 positivity correlated with menopause (55.56%) and lymph node metastasis (56.00%) (<i>p</i> < 0.05). No significant differences were found in age, reproductive history, family history, clinical stage, histologic grade, or T stage (<i>p</i> > 0.05). OS rates showed no significant difference, but PFS rates varied significantly (<i>p</i> < 0.05). Patients with high ERα36 expression in HER2-positive cases had the poorest prognosis (<i>p</i> < 0.05).</p><p><strong>Conclusion: </strong>ERα36 and HER2 are valuable prognostic biomarkers, with their co-expression linked to increased lymph node metastasis and poorer survival outcomes.</p>","PeriodicalId":9182,"journal":{"name":"Biomarkers in medicine","volume":" ","pages":"937-946"},"PeriodicalIF":2.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12622316/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145343389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endocan in nonalcoholic fatty liver disease (NAFLD): a systematic review and meta-analysis.","authors":"Amir Hossein Behnoush, Amirmohammad Khalaji, Elaheh Karimi, Zahra Shokri Varniab, Sai Gautham Kanagala, Alireza Yaghoobi, Filiz Mercantepe, Aleksandra Klisic","doi":"10.1080/17520363.2025.2585781","DOIUrl":"10.1080/17520363.2025.2585781","url":null,"abstract":"<p><strong>Background: </strong>Nonalcoholic fatty liver disease (NAFLD) is a common hepatic disorder linked to metabolic syndrome. Endothelial dysfunction is crucial in NAFLD progression. Endocan, a marker of endothelial injury, has been studied in NAFLD, and we aim to systematically evaluate these findings.</p><p><strong>Methods: </strong>PubMed, Scopus, Embase, and Web of Science were searched for studies measuring endocan levels in NAFLD patients compared to healthy controls. A random-effects meta-analysis was performed to estimate the standardized mean difference (SMD) with a 95% confidence interval (CI).</p><p><strong>Results: </strong>Ten studies with 1045 participants (mean age 44.1 ± 9.8 years, 48.8% male) met inclusion criteria. Meta-analysis showed no significant difference in endocan levels between NAFLD patients and controls (SMD 0.03, 95% CI -0.59 to 0.65, <i>p</i> = 0.931). The diagnostic accuracy of endocan for NAFLD varied (AUC 0.647-0.867). Endocan was linked to endothelial dysfunction, atherosclerosis, and coronary artery disease in NAFLD patients.</p><p><strong>Conclusion: </strong>Although serum endocan levels did not differ between NAFLD and controls, its role in endothelial dysfunction and disease prognosis suggests potential clinical relevance. Larger studies are needed to confirm these findings.</p>","PeriodicalId":9182,"journal":{"name":"Biomarkers in medicine","volume":" ","pages":"1027-1036"},"PeriodicalIF":2.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12645869/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145456977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Could the De Ritis ratio be a marker to predict the higher international normalized ratio values?","authors":"Osman Onur Kaplan, Mesut Engin, Seray Yazgan, Doğan Ormancı, Ahmet Kağan As, Ufuk Aydın, Yusuf Ata, Şenol Yavuz","doi":"10.1080/17520363.2025.2585782","DOIUrl":"10.1080/17520363.2025.2585782","url":null,"abstract":"<p><strong>Aim: </strong>In the present study, we aimed to investigate the prognostic importance of the De Ritis ratio [Aspartate Aminotransferase (AST)/Alanine Aminotransferase (ALT)] in predicting higher international normalized ratio in patients on warfarin treatment.</p><p><strong>Patients and methods: </strong>Patients who were followed up for warfarin use at the Training and Research Hospital, between September 2023 and September 2024, were included in this study, consecutively and retrospectively.</p><p><strong>Results: </strong>The study included a total of 357 consecutive patients, who were divided into three groups according to INR values: Group 1: Those with INR below 2, Group 2: Those with INR between 2 and 3.5, Group 3: INR values above 3.5. The median ages of the patients were 62 years (range, 22 to 88), 61 years (range, 24 to 87), and 61 years (range, 40 to 87), respectively (<i>p</i> = 0.833). There were significant differences between the groups regarding lymphocyte values (median: 2.1 × 10³/µL vs 2.1 × 10³/µL vs 1.6 × 10³/µL, respectively) and De Ritis ratio (median 1.03 vs 1.15 vs 1.92) (<i>p</i> = 0.005, and <i>p</i> < 0.001, respectively).</p><p><strong>Conclusion: </strong>We demonstrated a significant correlation between a high De Ritis ratio and high INR values. In light of this finding, a high De Ritis ratio may be indicative of elevated INR values for us.</p>","PeriodicalId":9182,"journal":{"name":"Biomarkers in medicine","volume":" ","pages":"971-977"},"PeriodicalIF":2.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12893730/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145450875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unique dysregulation of miR-7, miR-15, and miR-203 as novel biomarkers in Egyptian colorectal cancer.","authors":"Sally Farouk, Maha M Elbrashy, Rehab I Moustafa, Reem M El-Shenawy, Naiera M Helmy, Ahmed Khairy, Noha G Bader El Din","doi":"10.1080/17520363.2025.2585779","DOIUrl":"10.1080/17520363.2025.2585779","url":null,"abstract":"<p><strong>Background: </strong>Over the past decade, multiple research studies have focused on using miRNA expression in colorectal cancer (CRC) pathogenesis.</p><p><strong>Materials & methods: </strong>150 cases of two phases, where 14 miRNAs were first estimated in 75 cases for screening, followed by measuring miR-203a-3p, miR-7-5p, and miR-15b-5p relative expression in 75 subjects for further validation. Insilico analyses were done for confirmation.</p><p><strong>Results: </strong>miR-203a-3p and miR-15b-5p were significantly downregulated, whereas miR-7-5p was highly expressed in CRC patients. Significantly elevated diagnostic efficacy for CRC was observed for miR-203a-3p, miR-7-5p, and miR-15b-5p (<i>p</i> < 0.001). Serum expression levels of these miRNAs are positively correlated with their matched tissue expression levels (<i>p</i> < 0.001).</p><p><strong>Conclusion: </strong>Diagnostic biomarker potential was identified for miR-203a-3p, miR-7-5p, and miR-15b-5p in CRC.</p>","PeriodicalId":9182,"journal":{"name":"Biomarkers in medicine","volume":" ","pages":"999-1008"},"PeriodicalIF":2.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12893733/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145487804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum uric acid-creatinine ratio as a marker of chronic kidney disease: a retrospective observational study.","authors":"Ayşe Karaduru Avcı, Ayça İnci, Fatih Sargın, Burak Turgut, Esra Rızaoğulları Güzel, Semih Gül, Üstün Yılmaz","doi":"10.1080/17520363.2025.2586141","DOIUrl":"10.1080/17520363.2025.2586141","url":null,"abstract":"<p><strong>Objective: </strong>Serum uric acid (SUA) level has been associated with the progression of chronic kidney disease (CKD), but the role of the SUA/creatinine (SUA/Cre) ratio remains unclear. This study aimed to evaluate whether the SUA/Cre ratio is superior to isolated SUA as a marker for CKD.</p><p><strong>Methods: </strong>A retrospective observational study including 524 participants was conducted to compare SUA and SUA/Cre ratio in groups with and without CKD. Participants were categorized into 3 groups according to CKD stage: no CKD (<i>n</i> = 84), CKD stage 1-2 (<i>n</i> = 124), and CKD stage 3-4 (<i>n</i> = 316). Multivariate and receiver operating characteristic (ROC) analyses were performed to assess the association of SUA and SUA/Cre ratio with the presence of CKD.</p><p><strong>Results: </strong>According to the ROC curve, SUA level showed a stronger correlation than the SUA/Cre ratio (area under the curve [AUC] = 0.768 vs AUC = 0.261; <i>p</i> < .001).</p><p><strong>Conclusions: </strong>Although both the SUA/Cre ratio and SUA level were associated with renal dysfunction, SUA was found to be a better marker of CKD.</p>","PeriodicalId":9182,"journal":{"name":"Biomarkers in medicine","volume":" ","pages":"1019-1026"},"PeriodicalIF":2.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12893728/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145480937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A LASSO-based integrative model of serum biomarkers and gene polymorphisms for predicting poor sepsis prognosis.","authors":"Dalong Zhang, Huijun Dong, Yizhe Wang, Zhengtao Hao, Hanxiao Nie, Zhenzhen Wang, Ningyang Ding, Xingguo Niu","doi":"10.1080/17520363.2025.2586136","DOIUrl":"10.1080/17520363.2025.2586136","url":null,"abstract":"<p><strong>Background: </strong>The integration of serum biomarkers and gene polymorphisms may enhance early prognostic assessment in sepsis. Early and accurate prediction of outcomes is crucial for optimizing treatment strategies and improving survival. However, the clinical utility of combining genetic markers with conventional inflammatory indicators remains insufficiently validated.</p><p><strong>Methods: </strong>In this retrospective cohort (<i>n</i> = 81; July 2022-July 2024), patients were grouped by 28-day outcome. Candidate genes (TLR4, PPARγ, IL-12B, IL-27) were shortlisted from GSE54514. After data standardization and complete-case handling of < 5% missingness, ten-fold cross-validated LASSO selected predictors for multivariable logistic regression. Model performance was assessed by ROC AUC, Hosmer - Lemeshow (H - L) test, calibration plots, bootstrap optimism-correction (1,000 resamples), and decision-curve analysis (DCA).</p><p><strong>Results: </strong>6 predictors were retained - PCT, CRP, lactate (LAC), lactate clearance rate (LCR), TLR4 rs4986790, and PPARγ rs1801282. The nomogram achieved AUC 0.885 (95% CI 0.812-0.943) with sensitivity 88.6% and specificity 73.9%; calibration was good (H - L χ² = 9.191, <i>p</i> = 0.156). Bootstrap-corrected AUC was 0.872, with calibration slope 0.97 and Brier score 0.165. DCA indicated higher net benefit than SOFA or APACHE II across 0.05-0.40 threshold probabilities. In benchmarking, the integrative model outperformed SOFA and APACHE II (ΔAUC 0.129 and 0.104; DeLong <i>p</i> = 0.004 and 0.009) and showed higher net benefit on decision-curve analysis across 0.05-0.40 threshold probabilities.</p><p><strong>Conclusion: </strong>This integrative biomarker-genotype model demonstrated strong internal performance and potential clinical utility for individualized risk stratification in sepsis. The results support combining genetic susceptibility and inflammatory biomarkers for enhanced prognostic precision, although external and multi-ethnic validation remains warranted before widespread adoption.</p>","PeriodicalId":9182,"journal":{"name":"Biomarkers in medicine","volume":" ","pages":"1009-1018"},"PeriodicalIF":2.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12893731/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145522931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of serum biomarkers as prognostic indicators in pediatric cardiac arrest: a systematic review.","authors":"Maria Magalhães de Carvalho, Marta João Silva, Maria João Baptista","doi":"10.1080/17520363.2025.2574837","DOIUrl":"10.1080/17520363.2025.2574837","url":null,"abstract":"<p><strong>Purpose: </strong>After Cardiac Arrest (CA), serum biomarkers (SB) are released to the blood in response to brain damage and, therefore, may be helpful tools to predict neurological outcome. This systematic review aims at assessing the role of SB as neurological outcome indicators after pediatric CA.</p><p><strong>Methods: </strong>A systematic literature search was performed in PubMed, Scopus, WebOfScience and Cochrane Library. All information retrieved from the selected articles was summarized in a data table.</p><p><strong>Results: </strong>Eight studies, including a total of 539 patients, were analyzed. Neurological outcomes were assessed by using the Pediatric Cerebral Performance Category (PCPC) scale in all studies except one. Eleven serum biomarkers were evaluated, with higher concentrations observed in the unfavorable outcome group at various time points. However, only one study defined a specific cutoff value. The biomarkers with the highest predictive accuracy for neurological outcomes appear to be initial levels of IL-17 and CNFT, as well as NSE and S100B.</p><p><strong>Conclusion: </strong>Following pediatric CA, SB may be helpful indicators of unfavorable neurological outcome, as they seem to appear in higher concentrations in this group. Further research is required to establish specific timepoints and cutoff values and to determine the implication of these findings in clinical practice.</p>","PeriodicalId":9182,"journal":{"name":"Biomarkers in medicine","volume":" ","pages":"957-969"},"PeriodicalIF":2.1,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12622307/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145291143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic valuation of MMP family members as biomarkers for pancreatic cancer: a systematic review and meta-analysis.","authors":"Mengyuan Lu, Donglin Jiang, Jiaxin Zhang, Ling Liu, Yajing Shen, Tiandong Li, Peng Wang, Jianxiang Shi, Keyan Wang, Hua Ye","doi":"10.1080/17520363.2025.2571394","DOIUrl":"10.1080/17520363.2025.2571394","url":null,"abstract":"<p><strong>Background: </strong>There are numerous controversies surrounding the diagnosis of matrix metalloproteinases (MMPs) in pancreatic cancer (PC). Consequently, this study conducts the first comprehensive meta-analysis evaluating the diagnostic performance of MMP family members in PC, highlight potential biomarkers, and investigate sources of heterogeneity.</p><p><strong>Method: </strong>Articles on the MMP family and PC diagnosis were retrieved from PubMed and Web of Science. Diagnostic accuracy was assessed using sensitivity (SEN) and specificity (SPE). Threshold effects, heterogeneity (via meta-regression and subgroup analyses), and result robustness (via sensitivity analysis) were evaluated. Publication bias was examined using Deeks' funnel plot.</p><p><strong>Result: </strong>This meta-analysis included 48 studies from 23 articles, covering 12 types of MMP. The aggregated SEN, SPE, and area under the curve (AUC) for MMPs in diagnosing PC ranged from 0.61 to 0.76, 0.66 to 0.78, and 0.7273 to 0.8840, respectively. Meta-regression identified sample type as a major contributor to heterogeneity, with tissue-based MMPs showing markedly superior diagnostic performance compared to serum or plasma-based assays. Overall, MMPs showed potential diagnostic value, but their accuracy remains moderate and requires further validation in larger, well-designed studies.</p><p><strong>Conclusion: </strong>MMPs represent potential candidates for PC diagnosis, but current evidence is insufficient for clinical application, requiring further prospective studies.</p>","PeriodicalId":9182,"journal":{"name":"Biomarkers in medicine","volume":" ","pages":"909-919"},"PeriodicalIF":2.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12542613/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145306698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation between serum GFAP and 3-NT levels and the degree of neuronal injury in patients with glioma after surgery, and its diagnostic value.","authors":"Jiaode Jiang, Feng Liu","doi":"10.1080/17520363.2025.2559432","DOIUrl":"10.1080/17520363.2025.2559432","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the correlation between postoperative serum glial fibrillary acidic protein (GFAP) and 3-nitrotyrosine (3-NT) levels and neuronal injury severity in glioma patients.</p><p><strong>Methods: </strong>150 glioma patients were enrolled, with clinical baseline and pathological data recorded (age, sex, etc.). Neuronal injury was assessed using the National Institutes of Health Stroke Scale (NIHSS) postoperatively, categorizing patients into mild (NIHSS 1 ~ 4, <i>n</i> = 54) and moderate-to-severe (NIHSS ≥5, <i>n</i> = 96) groups. Serum GFAP and 3-NT levels were measured three days post-surgery via ELISA. Correlations with NIHSS were analyzed with Spearman's test. Patients were also stratified by median biomarker levels. Multivariate logistic regression identified severity risk factors. Receiver operating characteristic (ROC) curves evaluated diagnostic value.</p><p><strong>Results: </strong>GFAP and 3-NT levels were higher in the moderate-to-severe group (<i>p</i> < 0.001) and positively correlated with NIHSS scores (GFAP: <i>r</i> = 0.552; 3-NT: <i>r</i> = 0.545). Higher biomarker levels were significantly associated with worse injury (<i>p</i> < 0.001). Both were independent risk factors for severity. Combining GFAP and 3-NT predicted severity significantly better than either alone (<i>p</i> < 0.001).</p><p><strong>Conclusion: </strong>Postoperative serum GFAP and 3-NT levels correlate positively with neuronal injury severity in glioma patients. Their combination provides high diagnostic value for assessing postoperative injury severity.</p>","PeriodicalId":9182,"journal":{"name":"Biomarkers in medicine","volume":" ","pages":"837-846"},"PeriodicalIF":2.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12477868/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145028930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}