Biotechnology and Bioengineering最新文献_第4页

Cover Image, Volume 121, Number 11, November 2024 封面图片，第 121 卷第 11 期，2024 年 11 月

IF 3.5 2区生物学

Biotechnology and Bioengineering Pub Date : 2024-10-15 DOI: 10.1002/bit.28867

Jimmy Boman, Tjaša Marušič, Tina Vodopivec Seravalli, Janja Skok, Fredrik Pettersson, Kristina Šprinzar Nemec, Henrik Widmark, Rok Sekirnik

引用次数: 0

Biotechnology and Bioengineering: Volume 121, Number 11, November 2024 生物技术与生物工程第 121 卷第 11 号，2024 年 11 月

IF 3.5 2区生物学

Biotechnology and Bioengineering Pub Date : 2024-10-15 DOI: 10.1002/bit.28856

引用次数: 0

Blue-Purple evaluation: Chromoproteins facilitate the identification of BioBrick compatibility 蓝紫色评价：色素蛋白有助于鉴定生物特异性

IF 3.8 2区生物学

Biotechnology and Bioengineering Pub Date : 2024-10-14 DOI: 10.1002/bit.28862

Fang Ba, Yufei Zhang, Luyao Wang, Wan-Qiu Liu, Jian Li

{"title":"Blue-Purple evaluation: Chromoproteins facilitate the identification of BioBrick compatibility","authors":"Fang Ba, Yufei Zhang, Luyao Wang, Wan-Qiu Liu, Jian Li","doi":"10.1002/bit.28862","DOIUrl":"https://doi.org/10.1002/bit.28862","url":null,"abstract":"Synthetic BioBricks introduce novel capabilities to manipulate genetic information, direct transcription-translation processes, and program cellular behaviors in living organisms. To maintain the stability and functionality of synthetic BioBricks, assembled DNA fragments should be mutually compatible without inducing negative effects such as metabolic burden or cellular toxicity in host cells. However, a simple, rapid, and reliable method to evaluate BioBrick compatibility remains to be developed. In this study, we report BP (Blue/Purple, Ban/Pick) evaluation, a method utilizing chromoproteins to facilitate the identification of BioBrick compatibility in one-pot reactions. By visualizing and quantifying the ratio of blue to purple Escherichia coli (E. coli) colonies on LB-agar plates, we can easily validate the compatibility of desired BioBrick constructions. To demonstrate our design, we characterized BioBrick assemblies with antitoxin-toxin pair ccdA-ccdB, lysis protein E, or heterologous protein sfGFP. Among these, we successfully identified several compatible assemblies. We anticipate that BP evaluation will enhance biotechnological assessments of BioBrick compatibility in vivo and expand the application of chromoproteins in synthetic biology.","PeriodicalId":9168,"journal":{"name":"Biotechnology and Bioengineering","volume":"28 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142436435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Application and progress of 3D tumor models in breast cancer 三维肿瘤模型在乳腺癌中的应用和进展

IF 3.8 2区生物学

Biotechnology and Bioengineering Pub Date : 2024-10-14 DOI: 10.1002/bit.28860

Jiaojiao Xu, Wanxia Fang, Huanhuan Zhou, Ruiyuan Jiang, Zhanhong Chen, Xiaojia Wang

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Biosynthesis of pterostilbene in Escherichia coli from resveratrol on macroporous adsorption resin using a two-step substrate addition strategy 采用两步底物添加策略，在大肠杆菌中利用大孔吸附树脂从白藜芦醇中生物合成紫檀芪

IF 3.8 2区生物学

Biotechnology and Bioengineering Pub Date : 2024-10-14 DOI: 10.1002/bit.28865

Bao Yingling, Wu Xueqin, Chen Xiaolong

{"title":"Biosynthesis of pterostilbene in Escherichia coli from resveratrol on macroporous adsorption resin using a two-step substrate addition strategy","authors":"Bao Yingling, Wu Xueqin, Chen Xiaolong","doi":"10.1002/bit.28865","DOIUrl":"https://doi.org/10.1002/bit.28865","url":null,"abstract":"Pterostilbene (PST), a 3’,5’-O-methylated derivative of resveratrol (RSV), is a potent natural antioxidant produced by some plants in trace amounts as defense compound. It exhibits various health-promoting activities, such as anticancer, antiviral, and antimicrobial effects. Large-scale biosynthesis of PST is crucial due to the challenges associated with extracting it from plants. This study aims to develop an efficient method for PST production using an engineered Escherichia coli strain by feeding RSV as a precursor. We introduced a two-step substrate addition strategy combined with immobilized RSV (IMRSV) on macroporous adsorption resin (MAR) to enhance PST production. Five MARs were selected for RSV immobilization, and the substrate addition strategy and fermentation parameters for PST synthesis were optimized. A maximum PST concentration of 403 ± 9 mg/L was achieved, representing a 239% increase over the control, which in a one-step addition of free RSV. The PST titer reached 395 ± 24 mg/L in a 3-L bioreactor. In conclusion, the combination of a two-step substrate addition system and IMRSV is a promising approach for the economical and industrial-scale production of PST.","PeriodicalId":9168,"journal":{"name":"Biotechnology and Bioengineering","volume":"19 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142431837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PCR- and wash-free detection of serum miRNA via signaling probe hybridization 通过信号探针杂交以 PCR 和免清洗方式检测血清 miRNA

IF 3.8 2区生物学

Biotechnology and Bioengineering Pub Date : 2024-10-13 DOI: 10.1002/bit.28859

Haruka Uno, Hiyori Takeuchi, Ishin Abe, Tomoko Yoshino, Tomoyuki Taguchi, Yuko Hirakawa, Tadashi Matsunaga, Tsuyoshi Tanaka

引用次数: 0

Acetaminophen production in the edible, filamentous cyanobacterium Arthrospira platensis. 可食用丝状蓝藻 Arthrospira platensis 中的对乙酰氨基酚生产。

IF 3.5 2区生物学

Biotechnology and Bioengineering Pub Date : 2024-10-11 DOI: 10.1002/bit.28858

Jacob M Hilzinger, Skyler Friedline, Divya Sivanandan, Ya-Fang Cheng, Shunsuke Yamazaki, Douglas S Clark, Jeffrey M Skerker, Adam P Arkin

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Online monitoring of methane transfer rates unveils nitrogen fixation dynamics in Methylococcus capsulatus. 甲烷转移率的在线监测揭示了荚膜甲球菌的固氮动态。

IF 3.5 2区生物学

Biotechnology and Bioengineering Pub Date : 2024-10-11 DOI: 10.1002/bit.28855

Dominik Engel, Maximilian Hoffmann, Udo Kosfeld, Marcel Mann

{"title":"Online monitoring of methane transfer rates unveils nitrogen fixation dynamics in Methylococcus capsulatus.","authors":"Dominik Engel, Maximilian Hoffmann, Udo Kosfeld, Marcel Mann","doi":"10.1002/bit.28855","DOIUrl":"10.1002/bit.28855","url":null,"abstract":"This study explores methane utilization by the methanotrophic microorganism Methylococcus capsulatus (Bath) for biomass production, presenting a promising approach to mitigate methane emissions and foster the development sustainable biomaterials. Traditional screening methods for gas cultivations involve either serum flasks without online monitoring or costly, low-throughput fermenters. To address these limitations, the Respiration Activity MOnitoring System was augmented with methane sensors for real-time methane transfer rate (MTR) monitoring in shake flasks. Utilizing online monitoring of the MTR in shake flasks results in enhanced throughput and cost-effectiveness for cultivating M. capsulatus. Simultaneous monitoring of transfer rates for oxygen, methane, and carbon dioxide was conducted in up to eight shake flasks, ensuring the success of the cultivation process. Alterations in methane-to-oxygen transfer rate ratios and carbon fixation rates reveal the impact of transfer limitations on microbial growth. Detection of gas transfer limitations, exploration of process parameter influences, and investigations of medium components were enabled by the introduced method. Optimal nitrogen concentrations could be determined to ensure optimal growth. This streamlined approach accelerates the screening process, offering efficient investigations into metabolic effects, limitations, and parameter influences in gas fermentations without the need for elaborate offline sampling, significantly reducing costs and enhanced reproducibility.","PeriodicalId":9168,"journal":{"name":"Biotechnology and Bioengineering","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142399412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The proliferation and differentiation of skeletal muscle stem cells are enhanced in a bioreactor. 骨骼肌干细胞的增殖和分化在生物反应器中得到增强。

IF 3.5 2区生物学

Biotechnology and Bioengineering Pub Date : 2024-10-06 DOI: 10.1002/bit.28857

Wei-Hsuan Lin, Chung-Yuh Tzeng, Fan-Che Kao, Chia-Wen Tsao, Ning Li, Chuan-Che Wu, Sheng-Huei Lee, Kai-Fan Huang, Wei-Wen Hu, Shen-Liang Chen

{"title":"The proliferation and differentiation of skeletal muscle stem cells are enhanced in a bioreactor.","authors":"Wei-Hsuan Lin, Chung-Yuh Tzeng, Fan-Che Kao, Chia-Wen Tsao, Ning Li, Chuan-Che Wu, Sheng-Huei Lee, Kai-Fan Huang, Wei-Wen Hu, Shen-Liang Chen","doi":"10.1002/bit.28857","DOIUrl":"10.1002/bit.28857","url":null,"abstract":"Skeletal muscle (SKM) is the largest organ in mammalian body and it can repair damages by using the residential myogenic stem cells (MuSC), but this repairing capacity reduces with age and in some genetic muscular dystrophy. Under these circumstances, artificial amplification of autologous MuSC in vitro might be necessary to repair the damaged SKM. The amplification of MuSC is highly dependent on myogenic signals, such as sonic hedgehog (Shh), Wnt3a, and fibroblast growth factors, so formulating an optimum myogenic kit composed of specific myogenic signals might increase the proliferation and differentiation of MuSC efficiently. In this study, various myogenic signals have been tested on C2C12 myoblasts and primary MuSC, and a myogenic kit consists of insulin, lithium chloride, T3, and retinoic acid has been formulated, and we found it significantly increased the fusion index and MHC expression level of both C2C12 and MuSC myotubes. A novel bioreactor providing cyclic stretching (CS) and electrical stimulation (ES) has been fabricated to enhance the myogenic differentiation of both C2C12 and MuSC. We further found that coating the bioreactor substratum with collagen gave the best effect on proliferation and differentiation of MuSC. Furthermore, combining the collagen coating and physical stimuli (CS + ES) in the bioreactor can generate more proliferative primary MuSC cells. Our results have demonstrated that the combination of myogenic kit and bioreactor can provide environment for efficient MuSC proliferation and differentiation. These MuSC and mature myotubes amplified in the bioreactor might be useful for clinical grafting into damaged SKM in the future.","PeriodicalId":9168,"journal":{"name":"Biotechnology and Bioengineering","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142379100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biointerface engineering through amalgamation of gene technology and site-specific growth factor conjugation for efficient osteodifferentiation. 通过基因技术与特定位点生长因子结合的生物界面工程，实现高效骨分化。

IF 3.8 2区生物学

Biotechnology and Bioengineering Pub Date : 2024-09-19 DOI: 10.1002/bit.28852

Zhenxu Wu,Li Mo,Zongliang Wang,Liangsong Song,Eiry Kobatake,Yoshihiro Ito,Yi Wang,Peibiao Zhang

{"title":"Biointerface engineering through amalgamation of gene technology and site-specific growth factor conjugation for efficient osteodifferentiation.","authors":"Zhenxu Wu,Li Mo,Zongliang Wang,Liangsong Song,Eiry Kobatake,Yoshihiro Ito,Yi Wang,Peibiao Zhang","doi":"10.1002/bit.28852","DOIUrl":"https://doi.org/10.1002/bit.28852","url":null,"abstract":"The development of bone implants through bioinspired immobilization of growth factors remains a key issue in the generation of biological interfaces, especially in enhancing osteodifferentiation ability. In this study, we developed a strategy for surface functionalization of poly(lactide-glycolide) (PLGA) and hydroxyapatite (HA) composite substrates through site-specific conjugation of bone morphogenetic protein 2 containing 3,4-hydroxyphenalyalanine (DOPA-BMP2) mediated by tyrosinase and sortase A (SrtA). Firstly, the growth factor BMP2-LPETG containing LPETG motif was successfully expressed in Escherichia coli through recombinant DNA technology. The excellent binding affinity of binding growth factor (DOPA-BMP2) was achieved by converting the tyrosine residue (Y) of YKYKY-GGG peptide into DOPA (X) by tyrosinase, which bound to the substrates. Then its GGG motif was specifically bound to the end of BMP2-LPETG mediated by SrtA. Therefore, the generated bioactive DOPA-BMP2/PLGA/HA substrates significantly promoted the osteogenic differentiation of MC3T3-E1 cells. Thanks to this microbial-assisted engineering approach, our work presents a facile and highly site-specific strategy to engineer biomimetic materials for orthopedics and dentistry by effectively delivering growth factors, peptides, and other biomacromolecules.","PeriodicalId":9168,"journal":{"name":"Biotechnology and Bioengineering","volume":"11 1","pages":""},"PeriodicalIF":3.8,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142273585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0