Biotechnology and Bioengineering最新文献_第10页

Adjustment of the main biosynthesis modules to enhance the production of l-homoserine in Escherichia coli W3110 调整大肠杆菌 W3110 的主要生物合成模块以提高 l-高丝氨酸的产量

IF 3.5 2区生物学

Biotechnology and Bioengineering Pub Date : 2024-10-18 DOI: 10.1002/bit.28861

Kun Niu, Rui Zheng, Miao Zhang, Mao-Qin Chen, Yi-Ming Kong, Zhi-Qiang Liu, Yu-Guo Zheng

{"title":"Adjustment of the main biosynthesis modules to enhance the production of l-homoserine in Escherichia coli W3110","authors":"Kun Niu, Rui Zheng, Miao Zhang, Mao-Qin Chen, Yi-Ming Kong, Zhi-Qiang Liu, Yu-Guo Zheng","doi":"10.1002/bit.28861","DOIUrl":"10.1002/bit.28861","url":null,"abstract":"l-homoserine is an important platform compound of many valuable products. Construction of microbial cell factory for l-homoserine production from glucose has attracted a great deal of attention. In this study, l-homoserine biosynthesis pathway was divided into three modules, the glucose uptake and upstream pathway, the downstream pathway, and the energy supply module. Metabolomics of the chassis strain HS indicated that the supply of ATP was inadequate, therefore, the energy supply module was firstly modified. By balancing the ATP supply module, the l-homoserine production increased by 66% to 12.55 g/L. Further, the results indicated that the upstream pathway was blocked, and increasing the culture temperature to 37°C could solve this problem and the l-homoserine production reached 21.38 g/L. Then, the downstream synthesis pathways were further strengthened to balance the fluxes, and the l-homoserine production reached the highest reported level of 32.55 g/L in shake flasks. Finally, fed-batch fermentation in a 5-L bioreactor was conducted, and l-homoserine production could reach to 119.96 g/L after 92 h cultivation, with the yield of 0.41 g/g glucose and productivity of 1.31 g/L/h. The study provides a well research foundation for l-homoserine production by microbial fermentation with the capacity for industrial application.","PeriodicalId":9168,"journal":{"name":"Biotechnology and Bioengineering","volume":"122 1","pages":"223-232"},"PeriodicalIF":3.5,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142449589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transfer learning Bayesian optimization for competitor DNA molecule design for use in diagnostic assays 用于诊断分析的竞争对手 DNA 分子设计的迁移学习贝叶斯优化方法

IF 3.5 2区生物学

Biotechnology and Bioengineering Pub Date : 2024-10-16 DOI: 10.1002/bit.28854

Ruby Sedgwick, John P. Goertz, Molly M. Stevens, Ruth Misener, Mark van der Wilk

引用次数: 0

Reshaping the substrate-binding pocket of acyl-ACP reductase to enhance the production of sustainable aviation fuel in Escherichia coli 重塑酰基-ACP 还原酶的底物结合袋，提高大肠杆菌可持续航空燃料的产量

IF 3.5 2区生物学

Biotechnology and Bioengineering Pub Date : 2024-10-16 DOI: 10.1002/bit.28863

Jiahu Han, Takuya Matsumoto, Ryosuke Yamada, Hiroyasu Ogino

{"title":"Reshaping the substrate-binding pocket of acyl-ACP reductase to enhance the production of sustainable aviation fuel in Escherichia coli","authors":"Jiahu Han, Takuya Matsumoto, Ryosuke Yamada, Hiroyasu Ogino","doi":"10.1002/bit.28863","DOIUrl":"10.1002/bit.28863","url":null,"abstract":"To reduce carbon emissions and address environmental concerns, the aviation industry is exploring the use of sustainable aviation fuel (SAF) as an alternative to traditional fossil fuels. In this context, bio-alkane is considered a potentially high-value solution. The present study focuses on the enzymes acyl-acyl carrier protein [ACP] reductase (AAR) and aldehyde-deformylating oxygenase (ADO), which are crucial enzymes for alka(e)ne biosynthesis. By using protein engineering techniques, including semi-rational design and site-directed mutagenesis, we aimed to enhance the substrate specificity of AAR and improve alkane production efficiency. The co-expression of a modified AAR (Y26G/Q40M mutant) with wild-type ADO in Escherichia coli significantly increased alka(e)ne production from 28.92 mg/L to 167.30 mg/L, thus notably demonstrating a 36-fold increase in alkane yield. This research highlights the potential of protein engineering in optimizing SAF production, thereby contributing to the development of more sustainable and efficient SAF production methods and promoting greener air travel.","PeriodicalId":9168,"journal":{"name":"Biotechnology and Bioengineering","volume":"122 1","pages":"211-222"},"PeriodicalIF":3.5,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142444008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cover Image, Volume 121, Number 11, November 2024 封面图片，第 121 卷第 11 期，2024 年 11 月

IF 3.5 2区生物学

Biotechnology and Bioengineering Pub Date : 2024-10-15 DOI: 10.1002/bit.28867

Jimmy Boman, Tjaša Marušič, Tina Vodopivec Seravalli, Janja Skok, Fredrik Pettersson, Kristina Šprinzar Nemec, Henrik Widmark, Rok Sekirnik

引用次数: 0

Biotechnology and Bioengineering: Volume 121, Number 11, November 2024 生物技术与生物工程第 121 卷第 11 号，2024 年 11 月

IF 3.5 2区生物学

Biotechnology and Bioengineering Pub Date : 2024-10-15 DOI: 10.1002/bit.28856

引用次数: 0

Blue-Purple evaluation: Chromoproteins facilitate the identification of BioBrick compatibility 蓝紫色评价：色素蛋白有助于鉴定生物特异性

IF 3.5 2区生物学

Biotechnology and Bioengineering Pub Date : 2024-10-14 DOI: 10.1002/bit.28862

Fang Ba, Yufei Zhang, Luyao Wang, Wan-Qiu Liu, Jian Li

{"title":"Blue-Purple evaluation: Chromoproteins facilitate the identification of BioBrick compatibility","authors":"Fang Ba, Yufei Zhang, Luyao Wang, Wan-Qiu Liu, Jian Li","doi":"10.1002/bit.28862","DOIUrl":"10.1002/bit.28862","url":null,"abstract":"Synthetic BioBricks introduce novel capabilities to manipulate genetic information, direct transcription-translation processes, and program cellular behaviors in living organisms. To maintain the stability and functionality of synthetic BioBricks, assembled DNA fragments should be mutually compatible without inducing negative effects such as metabolic burden or cellular toxicity in host cells. However, a simple, rapid, and reliable method to evaluate BioBrick compatibility remains to be developed. In this study, we report BP (Blue/Purple, Ban/Pick) evaluation, a method utilizing chromoproteins to facilitate the identification of BioBrick compatibility in one-pot reactions. By visualizing and quantifying the ratio of blue to purple Escherichia coli (E. coli) colonies on LB-agar plates, we can easily validate the compatibility of desired BioBrick constructions. To demonstrate our design, we characterized BioBrick assemblies with antitoxin-toxin pair ccdA-ccdB, lysis protein E, or heterologous protein sfGFP. Among these, we successfully identified several compatible assemblies. We anticipate that BP evaluation will enhance biotechnological assessments of BioBrick compatibility in vivo and expand the application of chromoproteins in synthetic biology.","PeriodicalId":9168,"journal":{"name":"Biotechnology and Bioengineering","volume":"122 1","pages":"233-241"},"PeriodicalIF":3.5,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142436435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application and progress of 3D tumor models in breast cancer 三维肿瘤模型在乳腺癌中的应用和进展

IF 3.5 2区生物学

Biotechnology and Bioengineering Pub Date : 2024-10-14 DOI: 10.1002/bit.28860

Jiaojiao Xu, Wanxia Fang, Huanhuan Zhou, Ruiyuan Jiang, Zhanhong Chen, Xiaojia Wang

引用次数: 0

Biosynthesis of pterostilbene in Escherichia coli from resveratrol on macroporous adsorption resin using a two-step substrate addition strategy 采用两步底物添加策略，在大肠杆菌中利用大孔吸附树脂从白藜芦醇中生物合成紫檀芪

IF 3.5 2区生物学

Biotechnology and Bioengineering Pub Date : 2024-10-14 DOI: 10.1002/bit.28865

Bao Yingling, Wu Xueqin, Chen Xiaolong

{"title":"Biosynthesis of pterostilbene in Escherichia coli from resveratrol on macroporous adsorption resin using a two-step substrate addition strategy","authors":"Bao Yingling, Wu Xueqin, Chen Xiaolong","doi":"10.1002/bit.28865","DOIUrl":"10.1002/bit.28865","url":null,"abstract":"Pterostilbene (PST), a 3’,5’-O-methylated derivative of resveratrol (RSV), is a potent natural antioxidant produced by some plants in trace amounts as defense compound. It exhibits various health-promoting activities, such as anticancer, antiviral, and antimicrobial effects. Large-scale biosynthesis of PST is crucial due to the challenges associated with extracting it from plants. This study aims to develop an efficient method for PST production using an engineered Escherichia coli strain by feeding RSV as a precursor. We introduced a two-step substrate addition strategy combined with immobilized RSV (IMRSV) on macroporous adsorption resin (MAR) to enhance PST production. Five MARs were selected for RSV immobilization, and the substrate addition strategy and fermentation parameters for PST synthesis were optimized. A maximum PST concentration of 403 ± 9 mg/L was achieved, representing a 239% increase over the control, which in a one-step addition of free RSV. The PST titer reached 395 ± 24 mg/L in a 3-L bioreactor. In conclusion, the combination of a two-step substrate addition system and IMRSV is a promising approach for the economical and industrial-scale production of PST.","PeriodicalId":9168,"journal":{"name":"Biotechnology and Bioengineering","volume":"122 1","pages":"149-158"},"PeriodicalIF":3.5,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142431837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PCR- and wash-free detection of serum miRNA via signaling probe hybridization 通过信号探针杂交以 PCR 和免清洗方式检测血清 miRNA

IF 3.5 2区生物学

Biotechnology and Bioengineering Pub Date : 2024-10-13 DOI: 10.1002/bit.28859

Haruka Uno, Hiyori Takeuchi, Ishin Abe, Tomoko Yoshino, Tomoyuki Taguchi, Yuko Hirakawa, Tadashi Matsunaga, Tsuyoshi Tanaka

引用次数: 0

Acetaminophen production in the edible, filamentous cyanobacterium Arthrospira platensis 可食用丝状蓝藻 Arthrospira platensis 中的对乙酰氨基酚生产。

IF 3.5 2区生物学

Biotechnology and Bioengineering Pub Date : 2024-10-11 DOI: 10.1002/bit.28858

Jacob M. Hilzinger, Skyler Friedline, Divya Sivanandan, Ya-Fang Cheng, Shunsuke Yamazaki, Douglas S. Clark, Jeffrey M. Skerker, Adam P. Arkin

{"title":"Acetaminophen production in the edible, filamentous cyanobacterium Arthrospira platensis","authors":"Jacob M. Hilzinger, Skyler Friedline, Divya Sivanandan, Ya-Fang Cheng, Shunsuke Yamazaki, Douglas S. Clark, Jeffrey M. Skerker, Adam P. Arkin","doi":"10.1002/bit.28858","DOIUrl":"10.1002/bit.28858","url":null,"abstract":"Spirulina is the common name for the edible, nonheterocystous, filamentous cyanobacterium Arthrospira platensis that is grown industrially as a food supplement, animal feedstock, and pigment source. Although there are many applications for engineering this organism, until recently no genetic tools or reproducible transformation methods have been published. While recent work showed the production of a diversity of proteins in A. platensis, including single-domain antibodies for oral delivery, there remains a need for a modular, characterized genetic toolkit. Here, we independently establish a reproducible method for the transformation of A. platensis and engineer this bacterium to produce acetaminophen as proof-of-concept for small molecule production in an edible host. This work opens A. platensis to the wider scientific community for future engineering as a functional food for nutritional enhancement, modification of organoleptic traits, and production of pharmaceuticals for oral delivery.","PeriodicalId":9168,"journal":{"name":"Biotechnology and Bioengineering","volume":"122 1","pages":"44-52"},"PeriodicalIF":3.5,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11632167/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142399411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0