Journal of inflammatory bowel diseases & disorders最新文献_第3页

Comparing the Effects of Lidocaine/Paracetamol and Midazolam/Fentanyl as a Premedication on Pain Intensity and Hemodynamic Changes in Patients Undergoing Cataract Surgery With Topical Anesthesia: A Randomized Double-blinded Pilot Study 比较利多卡因/扑热息痛与咪达唑仑/芬太尼作为预用药对表面麻醉白内障手术患者疼痛强度和血流动力学变化的影响:一项随机双盲试点研究

Journal of inflammatory bowel diseases & disorders Pub Date : 2022-07-01 DOI: 10.32598/jid.26.2.5

Nasim Zarrin, Marzieh Beigom Khezri, Raheleh Safaei, M. Oladi

{"title":"Comparing the Effects of Lidocaine/Paracetamol and Midazolam/Fentanyl as a Premedication on Pain Intensity and Hemodynamic Changes in Patients Undergoing Cataract Surgery With Topical Anesthesia: A Randomized Double-blinded Pilot Study","authors":"Nasim Zarrin, Marzieh Beigom Khezri, Raheleh Safaei, M. Oladi","doi":"10.32598/jid.26.2.5","DOIUrl":"https://doi.org/10.32598/jid.26.2.5","url":null,"abstract":"Background: Several supplementary approaches have been used to increase the patient’s comfort during phacoemulsification under topical anesthesia. Objective: This study aimed to compare the effects of lidocaine/paracetamol (LP) and midazolam/fentanyl (MF) administration on pain intensity and hemodynamic changes in patients undergoing cataract surgery using phacoemulsification. Methods: This study was designed and implemented as a pilot randomized double-blinded clinical trial. A total number of 80 patients with cataracts scheduled for phacoemulsification were randomly assigned to two groups (40 subjects in each group) to receive lidocaine at a dose of 1.5 mg/kg and then infused with 1 g of paracetamol in 100 cc of normal saline (LP group) or midazolam 0.2 mg/kg and fentanyl 1.5 µg/kg (MF group). Hemodynamic parameters and sedation scores were measured before, 5, and 15 minutes after surgery, and then during recovery. Furthermore, pain (VAS), patient-surgeon satisfaction, propofol, and opioid consumption were all assessed. Findings: The sedation scores during recovery in the LP group were significantly lower (P= 0.04) than those in the MF group. Respiratory depression was also significantly lower (P<0.001) in the LP group compared to that infused by MF. According to other findings, no significant difference was observed between both study groups. Conclusion: The use of lidocaine-paracetamol as a supplementary approach for patients undergoing cataract surgery under topical anesthesia can cause better sedation scores with lower respiratory depression compared to the use of midazolam-fentanyl.","PeriodicalId":91544,"journal":{"name":"Journal of inflammatory bowel diseases & disorders","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78694863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Asbestos-related Lung Diseases: A Brief Update 石棉相关肺部疾病:简要更新

Journal of inflammatory bowel diseases & disorders Pub Date : 2022-07-01 DOI: 10.32598/jid.26.2.2

S. Jargin

{"title":"Asbestos-related Lung Diseases: A Brief Update","authors":"S. Jargin","doi":"10.32598/jid.26.2.2","DOIUrl":"https://doi.org/10.32598/jid.26.2.2","url":null,"abstract":"Health risks from asbestos exposures have been evaluated, considering past professional histories when exposures at workplaces were higher than today. A linear no-threshold (LNT) model has been applied, although its relevance is unproven. Fibers are often found in the lungs and pleura of deceased people. Fiber findings do not prove that a disease is caused by asbestos. It is reasonable to assume that a targeted search for mesothelioma and other asbestos-related conditions in asbestos workers resulted in an increased detection rate. Histological and immunohistochemical characteristics of malignant mesothelioma partly overlap with other cancers, which may contribute to the overdiagnosis in exposed populations. The etiology and differential diagnosis of malignant pleural mesothelioma as well as differences in carcinogenicity between different asbestos types are briefly discussed here. In the author’s opinion, current regulations applied in some countries are excessive and should be reconsidered based on independent research. The most promising way to obtain reliable information would be through lifelong bioassays. It can be reasonably assumed that the non-use of asbestos-containing brakes, fireproofing, insulation, etc. increases the harm caused by fires, traffic accidents, and armed conflicts.","PeriodicalId":91544,"journal":{"name":"Journal of inflammatory bowel diseases & disorders","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77237309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating Self-rated Health Among Health Workers During COVID-19 Pandemic in Northwest Iran 调查伊朗西北部COVID-19大流行期间卫生工作者的自评健康状况

Journal of inflammatory bowel diseases & disorders Pub Date : 2022-07-01 DOI: 10.32598/jid.26.2.6

M. Sheikhi, Z. Hosseinkhani, Ramesh Hoseinzadeh Khezri, Zohre Froozanfar, F. Khodamoradi

{"title":"Investigating Self-rated Health Among Health Workers During COVID-19 Pandemic in Northwest Iran","authors":"M. Sheikhi, Z. Hosseinkhani, Ramesh Hoseinzadeh Khezri, Zohre Froozanfar, F. Khodamoradi","doi":"10.32598/jid.26.2.6","DOIUrl":"https://doi.org/10.32598/jid.26.2.6","url":null,"abstract":"Background: Health promotion among health workers requires appropriate evidence of relevant determinants. Objective: This study was conducted to investigate the relationship between mental health and its measured covariates with self-rated health (SRH) among health workers during the COVID-19 pandemic in northwest Iran. Methods: In this cross-sectional study, we recruited 569 health workers from three educational hospitals of Qazvin University of Medical Sciences, Iran. From July 1 to September 5 (2020), participants were asked to provide demographic and clinical information and to complete the symptom checklist 90 (SCL-90) questionnaire. The relationship between suboptimal SRH with each of the SCL-90 domains and measured covariates were analyzed using logistic regression models. Findings: The Mean±SD age of participants was 34.73(7.95) years. A total of 148 health workers (26%) reported their suboptimal SRH, which was higher in women (odds ratio [OR]: 1.98; 95% confidence interval [CI]: 1.21, 3.24), as well as among participants without physical activity (OR: 2.14; 95% CI: 1.35, 3.39). Depression (OR: 2.13; 95% CI: 1.64, 2.77) and anxiety (OR: 3.76; 95% CI: 2.78, 5.09) showed significantly higher odds of suboptimal SRH. Also, other SCL-90 domains indicated a positive association with suboptimal SRH during the COVID-19 pandemic. Conclusion: Mental health, gender, and physical activity were significant variables related to SRH. The current findings suggest that we should pay attention to mental health problems and other important covariates during the COVID-19 epidemic. Hence, policymakers should consider this issue in health promotion programs for health workers.","PeriodicalId":91544,"journal":{"name":"Journal of inflammatory bowel diseases & disorders","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73323672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Statin Treatment on COVID-19 Patients’ Outcomes: A Randomized Double-blind Controlled Clinical Trial 他汀类药物治疗对COVID-19患者预后的影响:一项随机双盲对照临床试验

Journal of inflammatory bowel diseases & disorders Pub Date : 2022-07-01 DOI: 10.32598/jid.26.2.8

Rozita Behadori, S. Dodangeh, Seyedeh Azam Nabavi, A. Allami

{"title":"Effect of Statin Treatment on COVID-19 Patients’ Outcomes: A Randomized Double-blind Controlled Clinical Trial","authors":"Rozita Behadori, S. Dodangeh, Seyedeh Azam Nabavi, A. Allami","doi":"10.32598/jid.26.2.8","DOIUrl":"https://doi.org/10.32598/jid.26.2.8","url":null,"abstract":"Background: Statins may be protective against viral infection and have been suggested for the treatment of coronavirus disease 2019 (COVID-19). Objective: In this study, we aimed to evaluate the effect of atorvastatin on COVID-19 patients. Methods: Our study is a randomized double-blind controlled clinical trial that constitutes a population of COVID-19 patients admitted to Bu-Ali Sina Hospital in Qazvin, Iran, from May to August 2021. For the intervention and control groups, in addition to the national standard treatment, atorvastatin 40 mg tablet and placebo were daily administered for 7 days, respectively. A questionnaire including demographic characteristics, history of underlying diseases, vital signs, laboratory and imaging results, and outcome (alive, died) was completed on the first, third, and fifth days of hospitalization. Finally, the obtained data were analyzed by SPSS software, version 25. Findings: One hundred five patients with COVID-19 (62 females and 43 males, mean age 69 years) were studied. On days 3 and 5 after the intervention, no significant difference was observed between the groups in terms of vital signs, laboratory findings, hospitalization time, and need for intensive care unit hospitalization. However, 5.7% of patients in the atorvastatin group and 0% of patients in the control group died (P=0.243). Among the studied variables, C-reactive protein (P=0.227 vs P=0.002), blood urea nitrogen (P=0.055 vs P<0.001), and creatinine (P=0.598 vs P=0.013) decreased significantly in the statin group (no control group during days 0-5). Conclusion: There was no evidence about the harm and benefits of statin treatment during COVID-19 hospitalization.","PeriodicalId":91544,"journal":{"name":"Journal of inflammatory bowel diseases & disorders","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84333607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Impact of Late Secondary Hyperparathyroidism on Mortality in COVID-19 Patients: A Longitudinal Study 晚期继发性甲状旁腺功能亢进对COVID-19患者死亡率的影响:一项纵向研究

Journal of inflammatory bowel diseases & disorders Pub Date : 2022-07-01 DOI: 10.32598/jid.26.2.3

S. Hashemipour, Sabereh Afshar, Somaieh Kiani, Pouria Shahsavari, Milad Badri, Arefeh Ghobadi, Mohammad Reza Hadizadeh Khairkhahan

{"title":"The Impact of Late Secondary Hyperparathyroidism on Mortality in COVID-19 Patients: A Longitudinal Study","authors":"S. Hashemipour, Sabereh Afshar, Somaieh Kiani, Pouria Shahsavari, Milad Badri, Arefeh Ghobadi, Mohammad Reza Hadizadeh Khairkhahan","doi":"10.32598/jid.26.2.3","DOIUrl":"https://doi.org/10.32598/jid.26.2.3","url":null,"abstract":"Background: Adverse effects of high parathormone hormone (PTH) in critical illness have been described in some studies. Objective: The relationship between high PTH levels with mortality in hospitalized patients with COVID-19 was evaluated in the present study. Methods: A total of 123 patients were included in the study. The patients were evaluated in phase 1 (on admission) and phase 2 (days 4-6 of hospitalization). The patients were categorized into four groups based on the PTH status in both phases: normal PTH1/normal PTH2 (group 1), high PTH1/normal PTH2 (group 2), high PTH1/high PTH2 (group 3), and normal PTH1/high PTH2 (group 4). The multiple logistic regression analysis was performed to examine the independent association of late hyperparathyroidism with mortality. After excluding ineligible participants, 115 patients in phase 1 and 96 patients in phase 2 (days 4-6 of hospitalization) were evaluated. Findings: The level of phase 2 PTH in non-survivors was significantly higher than in survivors (57.5±40.9 pg/mL vs. 27.6±16.2 pg/mL, P=0.001). The mortality rate was significantly higher in high-PTH groups in phase 2 compared to normal-PTH groups in this phase (50% and 42.9% in groups 3 and 4 vs. 6.6% and 18.2% in PTH groups 1 and 2, respectively, P=0.007). Late hyperparathyroidism was associated with 11.4 times higher mortality risk (95% CI: 2.3-56.1, P=0.003). Conclusion: Late hyperparathyroidism remained a significant predictor of mortality after adjusting for the main PTH secretion modulators and disease severity. Late hyperparathyroidism is an independent and strong risk factor for mortality in COVID-19. Further studies are necessary to clarify the mechanisms involved.","PeriodicalId":91544,"journal":{"name":"Journal of inflammatory bowel diseases & disorders","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87245344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Role of Orexin-A in Water Intake in Water-deprived Rats 食欲素a在缺水大鼠饮水中的作用

Journal of inflammatory bowel diseases & disorders Pub Date : 2022-07-01 DOI: 10.32598/jid.26.2.4

Mohammad Hossain Esmaeili, Hashem Haghdost-Yazdi, M. Sofiabadi, Hasan Azhdari-Zarmehri, Ali Tishuri

{"title":"The Role of Orexin-A in Water Intake in Water-deprived Rats","authors":"Mohammad Hossain Esmaeili, Hashem Haghdost-Yazdi, M. Sofiabadi, Hasan Azhdari-Zarmehri, Ali Tishuri","doi":"10.32598/jid.26.2.4","DOIUrl":"https://doi.org/10.32598/jid.26.2.4","url":null,"abstract":"Background: The lateral hypothalamus (LH), which produces orexin, is vital for body solution homeostasis. Lateral hypothalamus (LH) lesion causes adipsia and its stimulation increases water intake. Objective: This study aimed to investigate the effect of an intracerebroventricular (ICV) injection of orexin-A (OXA) on water-drinking behavior in water-deprived rats. Metods: A total of 32 male Wistar rats (220–250 g) were used and divided into 4 groups, control (no injection), vehicle (Normal saline, 5 µL), OXA (30 µg/rat), SB-334867 (OXA receptor selective antagonist, 30 µg/rat). After the microinjection of drugs or vehicles in the right lateral ventricle, each rat was placed individually into the metabolic cage and the amount of water intake, the delay time for the first water intake, and the referral number for water intake were recorded for 4 h. Findings: This study showed that ICV administration of OXA increased both water intake and the referral number for water intake in water-deprived animals (P<0.05). On the other hand, ICV administration of SB-334867 decreased water intake compared to vehicle and control groups. Conclusion: It can be concluded that OXA has a regulatory role in water drinking behavior in water-depriving states.","PeriodicalId":91544,"journal":{"name":"Journal of inflammatory bowel diseases & disorders","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88693545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Global Prevalence of Diphyllobothrium in Dogs, and Cats: A Systematic Review and Meta-analysis 狗和猫中双叶门菌的全球流行:一项系统综述和荟萃分析

Journal of inflammatory bowel diseases & disorders Pub Date : 2022-03-01 DOI: 10.32598/jid.26.1.1

Aida Vafae Eslahi, Meysam Olfatifar, Fatemeh Barikbin, Leila Zaki, Milad Badri

{"title":"The Global Prevalence of Diphyllobothrium in Dogs, and Cats: A Systematic Review and Meta-analysis","authors":"Aida Vafae Eslahi, Meysam Olfatifar, Fatemeh Barikbin, Leila Zaki, Milad Badri","doi":"10.32598/jid.26.1.1","DOIUrl":"https://doi.org/10.32598/jid.26.1.1","url":null,"abstract":"Background: Fish tapeworms of the genus Diphyllobothrium are pseudophyllidean cestodes transmitted through the consumption of raw or inadequately cooked fish. Objective: The current systematic review and meta-analysis aim to estimate the global prevalence of Diphyllobothrium in dogs and cats based on published literature. Methods: Multiple English databases (PubMed, Scopus, ProQuest, Web of Science, and Google Scholar) were explored for relevant papers published until December 2021. Findings: Among the 37 studies that were included, 32 documented Diphyllobothrium infection in dogs and five in cats. The pooled prevalence (95% confidence interval) was 0.060% (0.030%-0.100%). The analysis based on country showed that the highest pooled prevalence in dogs and cats was observed in Bangladesh (0.250%, 0.149%-0.366%) and Indonesia (0.254%, 0.182%-0.333%), respectively. Based on the continent, Africa (0.109%, 0.017%-0.264%) and Asia (0.060%, 0%-0.345%) were the most common regions for infection in dogs and cats, respectively. Among different diagnostic methods, the highest pooled prevalence was related to molecular (0.661%, 0.573%-0.743%) and parasitological techniques (0.041%, 0%-0.217%) for dogs and cats’ studies, respectively. Conclusion: The findings show the importance of establishing a prevention and control measure focused on improving regular deworming and enhancing awareness of parasitic zoonotic diseases to minimize the transmission risk.","PeriodicalId":91544,"journal":{"name":"Journal of inflammatory bowel diseases & disorders","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77136802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Frequency of Human Papilloma Virus 16/18 Patients With Prostate Cancer by Polymerase Chain Reaction Method in Hospitals of Tabriz City, Iran 聚合酶链反应法检测伊朗大不里士市医院16/18前列腺癌患者人乳头瘤病毒感染率

Journal of inflammatory bowel diseases & disorders Pub Date : 2022-03-01 DOI: 10.32598/jid.26.1.2

Robabeh Faghany Baladehi, A. Bazmani, Mahin Ahangar Oskouee, Abolfazl Jafari-Sales, T. Pirzadeh, Behrouz Shokouhi, Mohammad Yousef Memar, Hossein Bannazadeh Baghi

{"title":"Frequency of Human Papilloma Virus 16/18 Patients With Prostate Cancer by Polymerase Chain Reaction Method in Hospitals of Tabriz City, Iran","authors":"Robabeh Faghany Baladehi, A. Bazmani, Mahin Ahangar Oskouee, Abolfazl Jafari-Sales, T. Pirzadeh, Behrouz Shokouhi, Mohammad Yousef Memar, Hossein Bannazadeh Baghi","doi":"10.32598/jid.26.1.2","DOIUrl":"https://doi.org/10.32598/jid.26.1.2","url":null,"abstract":"Background: Prostate cancer (PCa), as the fifth leading cause of death, is the second most common cancer diagnosis in men worldwide. Human papillomavirus (HPV) can potentially contribute to PCa development and chronic inflammation. HPV infection leads to malignant and benign lesions in the genital areas of men and women. The data on the role of HPV in PCa development is contradictory. Objective: This study aims to investigate the frequency of HPV in PCa samples in hospitals in Tabriz City, Iran. Methods: This study was conducted in a cross-sectional descriptive manner. Paraffin tissue blocks including 50 patients with primary prostate adenocarcinoma and 50 patients with benign prostatic hyperplasia (BPH) were selected from Tabriz hospitals. All samples were examined for the presence of HPV16/18 by the polymerase chain reaction (PCR) method. Findings: 3 out of 50 PCa patients are infected with HPV18. None of the benign and malignant prostate samples are infected with HPV 16. Therefore, in our study, no connection exists between HPV and PCa. The Mean±SD age of HPV positive samples was 61.33±11.50 years. Conclusion: The result of this research does not support the role of HPV in the development of PCa. Therefore, additional studies are necessary to clarify the possible role of HPV in prostate carcinogenesis.","PeriodicalId":91544,"journal":{"name":"Journal of inflammatory bowel diseases & disorders","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79955182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum Anti-heat Shock Protein 27 Antibody Titers in Patients With Dyslipidemia: A Population-based Case-control Study 血脂异常患者血清抗热休克蛋白27抗体滴度:一项基于人群的病例对照研究

Journal of inflammatory bowel diseases & disorders Pub Date : 2022-03-01 DOI: 10.32598/jid.26.1.7

F. Sadabadi, M. Shahi, Sousan Darroudi, Azam Rastgar Moghadam, Sahar Heidari-Bakavoli, Asma Porsa, Sara Saffar Soflaei, H. Esmaeili, M. Mouhebati, Ghodratollah Salehi Sangani, G. Ferns, Bibi Razieh Hoseini Farash, M. Ghayour-Mobarhan

{"title":"Serum Anti-heat Shock Protein 27 Antibody Titers in Patients With Dyslipidemia: A Population-based Case-control Study","authors":"F. Sadabadi, M. Shahi, Sousan Darroudi, Azam Rastgar Moghadam, Sahar Heidari-Bakavoli, Asma Porsa, Sara Saffar Soflaei, H. Esmaeili, M. Mouhebati, Ghodratollah Salehi Sangani, G. Ferns, Bibi Razieh Hoseini Farash, M. Ghayour-Mobarhan","doi":"10.32598/jid.26.1.7","DOIUrl":"https://doi.org/10.32598/jid.26.1.7","url":null,"abstract":"Background: Heat shock protein 27 (HSP27) is found in several cell types of adults, such as cardiomyocytes, and endothelial cells. It is expressed in response to different cellular stress conditions. HSP27 decreases the levels of reactive oxygen species (ROS) and dyslipidemia is closely associated with increased endothelial production of reactive oxygen species (ROS). Objective: Higher serum HSP27 antigen and anti-HSP27 antibodies have been reported in patients with unstable angina and myocardial infarction Methods: This population-based case-control study was conducted in 2018. We investigated serum anti-HSP27 antibody titers in all participants with dyslipidemia from the Mashhad stroke and heart atherosclerotic disorder (MASHAD) study (n=8141) and those who were healthy in terms of dyslipidemia (n=1637) using an in-house enzyme-linked immune sorbent assay (ELISA) in individuals with dyslipidemia. Findings: Anti-HSP27 titers were significantly lower in individuals with dyslipidemia compared to people without dyslipidemia (P=0.036). Conclusion: Our results revealed that the anti-HSP27 antibody titer was lower in the participants with dyslipidemia than in the negative group. However, there may be a confounding effect of drug therapy. In a subgroup of dyslipidemic subjects, we observed lower anti-HSP27 antibody titers in patients treated with some drugs (statins or corticosteroids, non-steroidal anti-inflammatory drugs [NSAIDs], or anti-diabetic and anti-hypertensive) compared to subjects untreated with these drugs.","PeriodicalId":91544,"journal":{"name":"Journal of inflammatory bowel diseases & disorders","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73365857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prediction of an Efficient Signal Peptide for Optimized Expression of Mycobacterium Tuberculosis Heparin-binding Hemagglutinin Gene in Periplasmic Compartment of Escherichia Coli: A Bioinformatics Investigation 预测结核分枝杆菌肝素结合血凝素基因在大肠杆菌质周室优化表达的有效信号肽:生物信息学研究

Journal of inflammatory bowel diseases & disorders Pub Date : 2022-03-01 DOI: 10.32598/jid.26.1.4

A. Forouharmehr, N. Shams, N. Nazifi, A. Jaydari, Ehsan Rashidian

{"title":"Prediction of an Efficient Signal Peptide for Optimized Expression of Mycobacterium Tuberculosis Heparin-binding Hemagglutinin Gene in Periplasmic Compartment of Escherichia Coli: A Bioinformatics Investigation","authors":"A. Forouharmehr, N. Shams, N. Nazifi, A. Jaydari, Ehsan Rashidian","doi":"10.32598/jid.26.1.4","DOIUrl":"https://doi.org/10.32598/jid.26.1.4","url":null,"abstract":"Background: The heparin-binding hemagglutinin (HBHA) protein belonging to Mycobacterium tuberculosis is known as a molecular adjuvant. Objective: Hence, the expression of this protein in the prokaryotic system is essential. Methods: To predict an appropriate signal peptide for the expression of the HBHA protein, 50 signal peptides were selected from the signal peptide database. Then, the crucial parameters of signal peptides, including the probability of signal peptide, different regions of signal peptides, physicochemical features, sorting of signal peptides, and sub-cellular location were completely investigated by reliable tools. After the best-predicted signal peptide was identified, it was linked to the HBHA protein, and its secondary structure, tertiary structure, and in silico cloning in pET21a (+) was assessed. Findings: The results of different evaluations confirmed that only 13 signal peptides passed all features, including clearance of N, H, and C regions, D-score >0.7, instability index >40, and periplasmic localization. Finally, based on D-scores, among these 13 signal peptides, the asr (acid shock RNA) peptide with D-score=90 was selected as the best-predicted signal peptide to apply. Moreover, the results showed that the secondary structure of the adjuvant linked to asr peptide contained 88.18% alpha helix and 9.5% random coil. Also, the results of in silico cloning showed that the nucleotide sequences of the adjuvant linked to the asr peptide were successfully cloned between BamHI and XhoI enzymes in the multiple cloning site of pET21a (+). Conclusion: The results of this study confirmed that the asr peptide can be used as an appropriate signal peptide for the expression of the HBHA protein in the prokaryotic system.","PeriodicalId":91544,"journal":{"name":"Journal of inflammatory bowel diseases & disorders","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86724069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0