The Role of Orexin-A in Water Intake in Water-deprived Rats

Journal of inflammatory bowel diseases & disorders Pub Date : 2022-07-01 DOI:10.32598/jid.26.2.4

Mohammad Hossain Esmaeili, Hashem Haghdost-Yazdi, M. Sofiabadi, Hasan Azhdari-Zarmehri, Ali Tishuri

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Abstract

Background: The lateral hypothalamus (LH), which produces orexin, is vital for body solution homeostasis. Lateral hypothalamus (LH) lesion causes adipsia and its stimulation increases water intake. Objective: This study aimed to investigate the effect of an intracerebroventricular (ICV) injection of orexin-A (OXA) on water-drinking behavior in water-deprived rats. Metods: A total of 32 male Wistar rats (220–250 g) were used and divided into 4 groups, control (no injection), vehicle (Normal saline, 5 µL), OXA (30 µg/rat), SB-334867 (OXA receptor selective antagonist, 30 µg/rat). After the microinjection of drugs or vehicles in the right lateral ventricle, each rat was placed individually into the metabolic cage and the amount of water intake, the delay time for the first water intake, and the referral number for water intake were recorded for 4 h. Findings: This study showed that ICV administration of OXA increased both water intake and the referral number for water intake in water-deprived animals (P<0.05). On the other hand, ICV administration of SB-334867 decreased water intake compared to vehicle and control groups. Conclusion: It can be concluded that OXA has a regulatory role in water drinking behavior in water-depriving states.

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食欲素a在缺水大鼠饮水中的作用

背景:产生食欲素的外侧下丘脑(LH)对体内溶液平衡至关重要。外侧下丘脑(LH)病变引起脂肪缺失，其刺激增加了水的摄入量。目的:探讨脑室注射食欲素a (OXA)对缺水大鼠饮水行为的影响。方法:选用雄性Wistar大鼠32只(220 ~ 250 g)，分为4组，对照组(不注射)、对照(生理盐水，5µL)、OXA(30µg/大鼠)、SB-334867 (OXA受体选择性拮抗剂，30µg/大鼠)。右侧脑室微量注射药物或载具后，将每只大鼠单独置于代谢笼中，记录4 h后大鼠的摄水量、第一次摄水量延迟时间和转介取水次数。结果:本研究显示，ICV给药OXA增加了失水动物的摄水量和转介取水次数(P<0.05)。另一方面，与对照组和对照组相比，ICV给药SB-334867减少了水摄入量。结论:缺氧状态下OXA对饮水行为具有调节作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal of inflammatory bowel diseases & disorders

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