BMC Pulmonary Medicine最新文献

{"title":"The clinical value and most informative threshold of polygenic risk score in the Quebec City Case-Control Asthma Cohort.","authors":"Martin Pariès, Stéphanie Bougeard, Aida Eslami, Zhonglin Li, Michel Laviolette, Louis-Philippe Boulet, Evelyne Vigneau, Yohan Bossé","doi":"10.1186/s12890-025-03486-3","DOIUrl":"10.1186/s12890-025-03486-3","url":null,"abstract":"<p><p>Genome-wide association studies (GWAS) have identified genetic variants robustly associated with asthma. A potential near-term clinical application is to calculate polygenic risk score (PRS) to improve disease risk prediction. The value of PRS, as part of numerous multi-source variables used to define asthma, remains unclear. This study aims to evaluate PRS and define most informative thresholds in relation to conventional clinical and physiological criteria of asthma using a multivariate statistical method. Clinical and genome-wide genotyping data were obtained from the Quebec City Case-Control Asthma Cohort (QCCCAC), which is an independent cohort from previous GWAS. PRS was derived using LDpred2 and integrated with other asthma phenotypes by means of Principal Component Analysis with Optimal Scaling (PCAOS). PRS was considered using 'ordinal level of scaling' to account for non-linear information. In two dimensional PCAOS space, the first component delineated individuals with and without asthma, whereas the severity of asthma was discerned on the second component. The positioning of high vs. low PRS in this space matched the presence and absence of airway hyperresponsiveness, showing that PRS delineated cases and controls at the same extent as a positive bronchial challenge test. The top 10% and the bottom 5% of the PRS were the most informative thresholds to define individuals at high and low genetic risk of asthma in this cohort. PRS used in a multivariate method offers a decision-making space similar to hyperresponsiveness in this cohort and highlights the most informative and asymmetrical thresholds to define high and low genetic risk of asthma.</p>","PeriodicalId":9148,"journal":{"name":"BMC Pulmonary Medicine","volume":"25 1","pages":"21"},"PeriodicalIF":2.6,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11734400/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143000181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and determinants of restrictive lung disorder among quarry workers at the Umuoghara quarry site, Ebonyi State, Nigeria: a cross-sectional study.","authors":"Ngozika Substance Ike-Samuel, Osaeloka C Ekwueme, Susan Chioma Udeh","doi":"10.1186/s12890-025-03497-0","DOIUrl":"10.1186/s12890-025-03497-0","url":null,"abstract":"<p><strong>Introduction: </strong>Respiratory disorders pose a serious health risk for quarry workers exposed to dust, as they are a leading source of morbidity and mortality globally, often resulting in irreversible lung conditions. This study assessed the prevalence and determinants of restrictive disorder among quarry workers in Umuoghara quarry site, Ebonyi State.</p><p><strong>Methods: </strong>This study was done on quarry workers at the Umuoghara quarry site, Ebonyi State. An analytical cross-sectional study design was adopted. Data was collected using a pre-tested semi-structured questionnaire among 300 quarry workers selected by simple random sampling method. Lung function test was performed using a spirometer- spirovit SPI schiller and data was analyzed with the use of IBM SPSS version 23.0. Pearson's chi-square test was used to find associations between variables. Binary logistic regression (multivariate analysis) was used to find the determinants of restrictive lung disorder at p < 0.05.</p><p><strong>Results: </strong>The prevalence of restrictive disorder was 14.3%. Working for more than 5 years (AOR = 2.880 at 95% CI = 1.234-6.720), Working for more than 10 years (AOR = 9.645 at 95% CI = 2.601-35.766), smoking (AOR = 3.558 at 95% CI = 1.631-7.762) and non-use of protective measures (AOR = 0.114; 95% CI = 0.050-0.262) were the determinants of restrictive lung disorder in quarry workers.</p><p><strong>Conclusion: </strong>There is an increased risk of developing respiratory problems among quarry workers exposed to quarry dust. It is recommended that employees receive thorough education on the dangers of this exposure, and that employers be mandated to provide protective equipment and strictly enforce its use among workers.</p>","PeriodicalId":9148,"journal":{"name":"BMC Pulmonary Medicine","volume":"25 1","pages":"22"},"PeriodicalIF":2.6,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11736936/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143000127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A retrospective study on potential drug‒drug interactions in patients with severe asthma receiving biological therapy: a single-center experience.","authors":"Mirna Momcilovic, Petra Turcic, Franka Butkovic, Sanja Popovic Grle","doi":"10.1186/s12890-025-03495-2","DOIUrl":"10.1186/s12890-025-03495-2","url":null,"abstract":"<p><strong>Background: </strong>Prevalence of potential drug-drug interactions (pDDIs) in adult patients with severe asthma on biological therapy and their clinical significance have not been fully addressed, thus the aim of this study was to investigate them.</p><p><strong>Methods: </strong>In this retrospective observational study, patients who were diagnosed with severe asthma and to whom biological therapy was prescribed between September 2015 and December 2020, were enrolled. The study was conducted at the Department of Allergic and Obstructive Pulmonary Diseases, Clinic for Lung Diseases Jordanovac, Clinical Hospital Center Zagreb. Data on demographic characteristics as well as concomitant medication were collected. The analysis of pDDIs was conducted via Lexicomp^® online software. Interactions of significance levels A and B were only recorded, while those of levels C, D and X were further analysed. The collected data was processed via Microsoft Excel 365 software.</p><p><strong>Results: </strong>60 adult patients, 60% female and 40% male, with median age of 56.2 years, were enrolled. The incidence of pDDIs was 86.67%. Total number of pDDIs detected was 518, out of which 43.24%, 45%, 4.44% and 7.3% of clinical significance B, C, D and X. Interactions of level C, D and X were recorded in, as follows: 83.33%, 25% and 33.33% patients with an average of 4.66, 1.53 and 1.9 interactions per patient. Only 13.33% of the patients had none of the potential clinically significant DDI. Most drug pairs contained at least one antiasthmatic drug. Muscarinic receptor antagonists, oral corticosteroids, β2 agonists and methylxanthines showed potential of entering into clinically significant DDIs, while leukotriene antagonists and biologicals showed no potential for the above.</p><p><strong>Conclusion: </strong>Prevalence of potential drug-drug interactions in patients with severe asthma on biological therapy is high. The majority of identified interactions have moderate to high level of clinical significance. Their identification, prevention and resolution could contribute to optimizing therapy, maximizing its therapeutic effect and avoiding undesirable adverse events.</p>","PeriodicalId":9148,"journal":{"name":"BMC Pulmonary Medicine","volume":"25 1","pages":"23"},"PeriodicalIF":2.6,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11737206/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143000078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single cell transcriptomics in blood of patients with chronic obstructive pulmonary disease.","authors":"Yeonjeong Heo, Jeeyoung Kim, Seok-Ho Hong, Woo Jin Kim","doi":"10.1186/s12890-024-03475-y","DOIUrl":"10.1186/s12890-024-03475-y","url":null,"abstract":"<p><strong>Background: </strong>Chronic obstructive pulmonary disease (COPD) is a leading cause of morbidity and mortality worldwide. Single-cell RNA sequencing (scRNA-seq) provides gene expression profiles at the single-cell level. Hence, we evaluated gene expression in the peripheral blood of patients with COPD.</p><p><strong>Methods: </strong>Peripheral blood samples from seven healthy controls and eight patients with COPD were obtained in this study. The 10X Genomics Chromium Instrument and cDNA synthesis kit were utilized to generate a barcoded cDNA library for single cell RNA-sequencing. We compared the scRNA-seq data between the COPD and control groups using computational analysis. Functional analyses were performed using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses.</p><p><strong>Results: </strong>scRNA-seq was used to analyze the transcriptome of peripheral blood mononuclear cells from seven normal controls and eight patients with COPD. We found an increased number of monocyte/macrophages in the COPD group compared to the normal control group. Among the differentially expressed genes (DEGs) in monocyte/macrophages, we identified 15 upregulated genes (EGR1, NR4A1, CCL3, CXCL8, PTGS2, CD83, BCL2A1, SGK1, IL1B, BTG2, NFKBIZ, DUSP2, MAFB, PLAUR and CCL3L1) and 7 downregulated genes (FOLR3, RPS4Y1, HLA-DRB5, NAMPT, CD52, TMEM176A and TMEM176B) in the COPD group compared to the normal control group.</p><p><strong>Conclusions: </strong>Using scRNA-seq, we found differences in cell type distribution, especially in monocyte/ macrophages. Several upregulated and downregulated genes were found in the monocyte/macrophages of the COPD group.</p>","PeriodicalId":9148,"journal":{"name":"BMC Pulmonary Medicine","volume":"25 1","pages":"19"},"PeriodicalIF":2.6,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11734329/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of pan-immune inflammation value and lung health in adults.","authors":"Ya Lin, Xiao Lin, Chufan Ren, Lanlan Song, Chao Gu","doi":"10.1186/s12890-025-03493-4","DOIUrl":"10.1186/s12890-025-03493-4","url":null,"abstract":"<p><strong>Background: </strong>Lung health is intricately linked with inflammation. The pan-immune-inflammation value (PIV) emerges as a promising biomarker, offering reflection into systemic inflammatory states and assisting in the prognosis of diverse diseases. This research aims to explore the associations between PIV and respiratory symptoms, respiratory diseases and lung function.</p><p><strong>Methods: </strong>The study was a cross-sectional population study from the National Health and Nutrition Examination Survey (NHANES). Restricted cubic spline (RCS) models were conducted to explore the relationships between PIV and respiratory health outcomes, while weighted linear regression models and weighted logistic regression models were the ones used for regression analysis. Trend tests probed the evolving relationship among PIV quartiles and outcomes. The study incorporated subgroup analysis and interaction tests to examine associations within specific subpopulations.</p><p><strong>Results: </strong>From the cohort of 6,263 participants, a distinct negative correlation was identified between PIV and lung health. Subsequent to confounding factors, a 100-unit increment in PIV was linked to a 2% increase in the incidence of cough and phlegm (OR, 95% CI: 1.02, 1.00 to 1.05; 1.02, 1.00 to 1.04). Additionally, higher PIV was associated with reductions in FEV1 (MD, 95% CI: -5.37, -9.10 to -1.64) and FVC (MD, 95% CI: -5.75, -10.34 to -1.15). Categorizing PIV into quartiles revealed an ascending trend: A significantly higher risk of cough/phlegm/wheeze was found in participants in the second/third/fourth PIV quartile compared to those in the first PIV quartile (all p for trend < 0.05). Moreover, lung function indicators (FEV1, FEV1%, FVC, FVC%, FEV1/FVC) declined significantly in the fourth quartile (all p for trend < 0.05). Besides, a nonlinear relationship between PIV and outcomes was evident. Subgroup analysis revealed variations in these associations stratified by gender, age, smoking and drinking status, as well as certain disease history.</p><p><strong>Conclusions: </strong>The study highlighted the potential connections between PIV and respiratory symptoms, respiratory diseases and lung function. Monitoring PIV level could provide valuable insights into the inflammatory status and may inform clinical approaches for managing respiratory health.</p>","PeriodicalId":9148,"journal":{"name":"BMC Pulmonary Medicine","volume":"25 1","pages":"18"},"PeriodicalIF":2.6,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11734563/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An exploratory analysis of differences in serum protein expression by sex in patients with systemic sclerosis associated interstitial lung disease.","authors":"Giuliana Cerro-Chiang, Matthew Ayres, Alejandro Rivas, Sarah J Parker, Mitra Mastali, Peter Chen, Jennifer E Van Eyk, Paul J Wolters, Francesco Boin, Tanzira Zaman","doi":"10.1186/s12890-024-03474-z","DOIUrl":"10.1186/s12890-024-03474-z","url":null,"abstract":"<p><strong>Background: </strong>Systemic sclerosis (SSc) is a rare connective tissue disease, frequently affecting the skin, lungs, and pulmonary vasculature. Approximately 30-50% of SSc patients develop interstitial lung disease (SSc-ILD), with 30-35% of related deaths attributed to it. Even though men are less likely to develop systemic sclerosis, they have a higher incidence of SSc-ILD than women, and they tend to develop it at a younger age with a higher mortality rate. Sex differences in protein expression in the blood of patients with SSc-ILD have not been reported to date. We aimed to identify sex differences in serum protein expression between men and women with SSc-ILD.</p><p><strong>Methods: </strong>Serum specimens of patients with SSc-ILD underwent dual mass spectrometry (LC-MS/MS) analysis. The association between protein biomarkers and sex was assessed through logistic regression. Time to event analysis was performed to determine any differences in the time to FVC decline of > 5% and the proportion of subjects who experienced FVC decline of > 5% by sex over the total period of observation. The association between biomarkers and sex was assessed through logistic regression. For proteins that were dichotomized, chi-squared testing was used. Multivariable regression models adjusting for meaningful clinical variables were also performed.</p><p><strong>Results: </strong>The cohort consisted of 211 subjects, 162 women and 47 men with a median follow-up of 3.52 years. No significant sex differences were found in the time to FVC decline of > 5% or > 10%. Among the 704 proteins identified, forty differed significantly between sexes. After adjusting for multiple testing, Autotaxin remained significantly higher in women. Autotaxin, known to activate lysophosphatidic acid and promote fibrosis, suggests a potential role in modulating fibrotic processes in SSc-ILD.</p><p><strong>Conclusions: </strong>This study is the first to report sex-specific serum protein differences in patients with SSc-ILD, with Autotaxin remaining significantly different after adjusting for multiple testing. These proteins could influence disease progression and treatment response and underscore the importance of personalized therapeutic strategies and further research into sex-related molecular pathways in SSc-ILD.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":9148,"journal":{"name":"BMC Pulmonary Medicine","volume":"25 1","pages":"16"},"PeriodicalIF":2.6,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11727626/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142977088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of myrtenol against paraquat-induced toxicity in rats.","authors":"Fatemeh Amin, Hosein Basirat, Najmeh Parvaz, Morteza Khademalhosseini, Elham Hakimizadeh, Iman Fatemi","doi":"10.1186/s12890-025-03484-5","DOIUrl":"10.1186/s12890-025-03484-5","url":null,"abstract":"<p><strong>Background: </strong>Paraquat (PQ) is a widely used pesticide, can cause severe intoxication and respiratory failure. Myrtenol (Mrl), an essential oil derived in various plants, exhibits several biological properties, including anti-inflammatory and antioxidant activities. This study aims to investigate the protective potential of Mrl against oxidative stress and inflammation caused by PQ exposure.</p><p><strong>Methods: </strong>Twenty-five Wistar albino rats were divided into the following groups (n = 5 in each group): a control group (treated by dimethyl sulfoxide (DMSO)), a PQ group (exposed to 54 mg/m³ aerosol PQ), and two treatment groups that were exposed to PQ aerosol and administered oral Mrl at doses of 25 mg/kg/day and 50 mg/kg/day, respectively. The final group was exposed to PQ aerosol and treated with oral dexamethasone at a dose of 0.03 mg/kg/day. Various hematological, oxidative, inflammatory, and pathological indices were measured at the conclusion of the treatment period.</p><p><strong>Results: </strong>PQ decreases the levels or activities of superoxide dismutase (SOD), catalase (CAT), and Thiol, while increasing the levels or activities of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and malondialdehyde (MDA). Mrl restored activites of SOD, and CAT, as well as thiol levels to near-control values while reducing TNF-α, IL-6, and MDA levels. Pathological studies further confirmed the therapeutic effects of Mrl.</p><p><strong>Conclusion: </strong>The results of this study demonstrate the promising therapeutic effects of Mrl against inhaled PQ in rats.</p>","PeriodicalId":9148,"journal":{"name":"BMC Pulmonary Medicine","volume":"25 1","pages":"17"},"PeriodicalIF":2.6,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11730473/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142977099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maximizing phonation: impact of inspiratory muscle strengthening on vocal durations and pitch range.","authors":"Coşkun Yilmaz, Özgür Bostanci, Özgür Eken, Rania Alkahtani, Monira I Aldhahi","doi":"10.1186/s12890-024-03471-2","DOIUrl":"10.1186/s12890-024-03471-2","url":null,"abstract":"<p><strong>Background: </strong>This study investigated the acute effects of inspiratory muscle warm-up (IWU) on vocal performance in singers. Proper vocal and respiratory warm-up can enhance vocal range, quality, and endurance. The aim was to determine whether IWU improves maximum phonation time and pitch range, contributing to better voice production efficiency (vocal efficiency) and reduced fatigue.</p><p><strong>Materials and methods: </strong>Singers were selected from the Samsun State Opera and the Ballet Directorate (n = 16). This cross-sectional study aimed to investigate the acute effects. The singers in the control group (SC = 8) performed only one session of routine voice warm-up, and the experimental group (SE = 8) conducted an inspiratory muscle warm-up (IWU) of 2 sets, 30 times/set at 40% maximal inspiratory pressure (MIP) in addition to routine voice warm-up. Subsequently, All participants were then required to perform pre- and post- pulmonary function tests, maximal inspiratory pressure (MIP) and maximal expiratory pressure (MEP), and voice recordings (note high pitch, note low pitch, high pitch durations and low pitch durations sustained with one breath, and maximum phonation duration).</p><p><strong>Results: </strong>All pulmonary function and muscle strength parameters improved in the SE group, with the highest increases in MIP (22.9%) and MEP (14.7%). No significant improvements were noted in the SC group (p > 0.05). The Borg Rating of Perceived Exertion showed that the SE group experienced less difficulty with their vocal performance after IWU (-11.6%, p = 0.006), while no significant change was observed in the SC group (p = 0.316). Both warm-up methods used in the study significantly affected the frequencies of high-pitch sounds (SE = 17.8%, SC = 10.9%, p = 0.003); however, the frequency of low-pitch sounds was not significantly affected (p = 0.437). IWU significantly affected the high-pitched note duration (p < 0.001; 32.17%), low-pitched note duration (p < 0.001; 27.11%), and maximum phonation time (p < 0.001; 21%), while routine voice warm-up did not significantly affect any parameter (p > 0.05).</p><p><strong>Conclusions: </strong>The combination of IWU with the general body and voice warm-up protocol can acutely improve vocal performance in terms of maximum phonation time, phonation times of the highest and lowest pitched sounds in a single breath, and vocal range levels.</p>","PeriodicalId":9148,"journal":{"name":"BMC Pulmonary Medicine","volume":"25 1","pages":"15"},"PeriodicalIF":2.6,"publicationDate":"2025-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725213/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142968826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological characterization and clinical significance of cuproptosis-related genes in lung adenocarcinoma.","authors":"Meilin Li, Yu Tan, Zhixin Li, Lingfeng Min","doi":"10.1186/s12890-025-03477-4","DOIUrl":"10.1186/s12890-025-03477-4","url":null,"abstract":"<p><strong>Background: </strong>Lung cancer has high morbidity and mortality rates, which results in a poor prognosis. Cuproptosis is a novel cell death mechanism. The aim of this study was to examine the biological characteristics and clinical significance of genes associated with cuproptosis in lung adenocarcinoma (LUAD), and to understand the molecular mechanisms underlying the occurrence and progression of LUAD.</p><p><strong>Methods: </strong>We targeted 10 cuproptosis-related genes from previous studies and used the datasets from GEO and TCGA databases to identify differential genes related to cuproptosis; then the data were analyzed by R package, Cytoscape, TISDB, cBioPortal, STRING, CancerSEA, and Disgenet; and finally, the data were detected by immunohistochemistry validation was performed.</p><p><strong>Results: </strong>CDKN2A and MTF1 were cuproptosis-associated LUAD differential genes and were differentially expressed in immune subtypes. The expression of CDKN2A and MTF1 showed correlation with multiple functional states of LUAD.CDKN2A was negatively correlated with LUAD survival prognosis.</p><p><strong>Conclusion: </strong>CDKN2A and MTF1 were correlated with the diagnosis of LUAD, and CDKN2A was negatively correlated with the survival and prognosis of LUAD. CDKN2A has the potential to contribute to the early diagnosis and prognosis analysis of LUAD.</p>","PeriodicalId":9148,"journal":{"name":"BMC Pulmonary Medicine","volume":"25 1","pages":"13"},"PeriodicalIF":2.6,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725195/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive analysis pinpoints CCNA2 as a prognostic and immunological biomarker in non-small cell lung cancer.","authors":"Liming Zhang, Shaoqiang Wang, Lina Wang","doi":"10.1186/s12890-025-03490-7","DOIUrl":"10.1186/s12890-025-03490-7","url":null,"abstract":"<p><strong>Background: </strong>Lung cancer is a leading cause of morbidity and mortality globally. Despite advances in targeted and immunotherapies, overall survival (OS) rates remain suboptimal. Cyclin-A2 (CCNA2), known for its upregulation in various tumors and role in tumorigenesis, has an undefined function in non-small cell lung cancer (NSCLC).</p><p><strong>Methods: </strong>We analyzed three microarray datasets from the Gene Expression Omnibus (GEO) repository to identify differentially expressed genes. Using STRING, we constructed a protein-protein interaction (PPI) network to pinpoint hub genes. The expression and prognostic relevance of CCNA2 were validated using GEPIA and the Kaplan-Meier plotter. Clinicopathological correlations were assessed via the Human Protein Atlas (HPA) and UALCAN databases. qRT-PCR and immunohistochemistry (IHC) were performed to validate CCNA2 mRNA and protein levels. Loss-of-function assays in lung cancer cell lines evaluated the biological role of CCNA2. Immune infiltration and single-cell sequencing were also explored.</p><p><strong>Results: </strong>Analysis of GSE18842, GSE101929, and GSE116959 datasets identified 321 upregulated and 623 downregulated genes in NSCLC. CCNA2 was confirmed to be highly expressed in NSCLC through qRT-PCR and IHC, with overexpression correlating with advanced pathological stages and lymph node metastasis. The area under the curve (AUC) of CCNA2 indicating high diagnostic accuracy. Immune infiltration and single-cell sequencing revealed that CCNA2 expression was significantly associated with immune cell infiltration, particularly in Tprolif cells.</p><p><strong>Conclusion: </strong>CCNA2 is upregulated in NSCLC and shows significant correlation with clinicopathological characteristics. Our findings suggest that CCNA2 may serve as a promising biomarker for both the prognosis and diagnosis of NSCLC.</p>","PeriodicalId":9148,"journal":{"name":"BMC Pulmonary Medicine","volume":"25 1","pages":"14"},"PeriodicalIF":2.6,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725219/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}