BMC Pulmonary Medicine最新文献

{"title":"Single cell transcriptomics in blood of patients with chronic obstructive pulmonary disease.","authors":"Yeonjeong Heo, Jeeyoung Kim, Seok-Ho Hong, Woo Jin Kim","doi":"10.1186/s12890-024-03475-y","DOIUrl":"https://doi.org/10.1186/s12890-024-03475-y","url":null,"abstract":"<p><strong>Background: </strong>Chronic obstructive pulmonary disease (COPD) is a leading cause of morbidity and mortality worldwide. Single-cell RNA sequencing (scRNA-seq) provides gene expression profiles at the single-cell level. Hence, we evaluated gene expression in the peripheral blood of patients with COPD.</p><p><strong>Methods: </strong>Peripheral blood samples from seven healthy controls and eight patients with COPD were obtained in this study. The 10X Genomics Chromium Instrument and cDNA synthesis kit were utilized to generate a barcoded cDNA library for single cell RNA-sequencing. We compared the scRNA-seq data between the COPD and control groups using computational analysis. Functional analyses were performed using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses.</p><p><strong>Results: </strong>scRNA-seq was used to analyze the transcriptome of peripheral blood mononuclear cells from seven normal controls and eight patients with COPD. We found an increased number of monocyte/macrophages in the COPD group compared to the normal control group. Among the differentially expressed genes (DEGs) in monocyte/macrophages, we identified 15 upregulated genes (EGR1, NR4A1, CCL3, CXCL8, PTGS2, CD83, BCL2A1, SGK1, IL1B, BTG2, NFKBIZ, DUSP2, MAFB, PLAUR and CCL3L1) and 7 downregulated genes (FOLR3, RPS4Y1, HLA-DRB5, NAMPT, CD52, TMEM176A and TMEM176B) in the COPD group compared to the normal control group.</p><p><strong>Conclusions: </strong>Using scRNA-seq, we found differences in cell type distribution, especially in monocyte/ macrophages. Several upregulated and downregulated genes were found in the monocyte/macrophages of the COPD group.</p>","PeriodicalId":9148,"journal":{"name":"BMC Pulmonary Medicine","volume":"25 1","pages":"19"},"PeriodicalIF":2.6,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of pan-immune inflammation value and lung health in adults.","authors":"Ya Lin, Xiao Lin, Chufan Ren, Lanlan Song, Chao Gu","doi":"10.1186/s12890-025-03493-4","DOIUrl":"https://doi.org/10.1186/s12890-025-03493-4","url":null,"abstract":"<p><strong>Background: </strong>Lung health is intricately linked with inflammation. The pan-immune-inflammation value (PIV) emerges as a promising biomarker, offering reflection into systemic inflammatory states and assisting in the prognosis of diverse diseases. This research aims to explore the associations between PIV and respiratory symptoms, respiratory diseases and lung function.</p><p><strong>Methods: </strong>The study was a cross-sectional population study from the National Health and Nutrition Examination Survey (NHANES). Restricted cubic spline (RCS) models were conducted to explore the relationships between PIV and respiratory health outcomes, while weighted linear regression models and weighted logistic regression models were the ones used for regression analysis. Trend tests probed the evolving relationship among PIV quartiles and outcomes. The study incorporated subgroup analysis and interaction tests to examine associations within specific subpopulations.</p><p><strong>Results: </strong>From the cohort of 6,263 participants, a distinct negative correlation was identified between PIV and lung health. Subsequent to confounding factors, a 100-unit increment in PIV was linked to a 2% increase in the incidence of cough and phlegm (OR, 95% CI: 1.02, 1.00 to 1.05; 1.02, 1.00 to 1.04). Additionally, higher PIV was associated with reductions in FEV1 (MD, 95% CI: -5.37, -9.10 to -1.64) and FVC (MD, 95% CI: -5.75, -10.34 to -1.15). Categorizing PIV into quartiles revealed an ascending trend: A significantly higher risk of cough/phlegm/wheeze was found in participants in the second/third/fourth PIV quartile compared to those in the first PIV quartile (all p for trend < 0.05). Moreover, lung function indicators (FEV1, FEV1%, FVC, FVC%, FEV1/FVC) declined significantly in the fourth quartile (all p for trend < 0.05). Besides, a nonlinear relationship between PIV and outcomes was evident. Subgroup analysis revealed variations in these associations stratified by gender, age, smoking and drinking status, as well as certain disease history.</p><p><strong>Conclusions: </strong>The study highlighted the potential connections between PIV and respiratory symptoms, respiratory diseases and lung function. Monitoring PIV level could provide valuable insights into the inflammatory status and may inform clinical approaches for managing respiratory health.</p>","PeriodicalId":9148,"journal":{"name":"BMC Pulmonary Medicine","volume":"25 1","pages":"18"},"PeriodicalIF":2.6,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An exploratory analysis of differences in serum protein expression by sex in patients with systemic sclerosis associated interstitial lung disease.","authors":"Giuliana Cerro-Chiang, Matthew Ayres, Alejandro Rivas, Sarah J Parker, Mitra Mastali, Peter Chen, Jennifer E Van Eyk, Paul J Wolters, Francesco Boin, Tanzira Zaman","doi":"10.1186/s12890-024-03474-z","DOIUrl":"https://doi.org/10.1186/s12890-024-03474-z","url":null,"abstract":"<p><strong>Background: </strong>Systemic sclerosis (SSc) is a rare connective tissue disease, frequently affecting the skin, lungs, and pulmonary vasculature. Approximately 30-50% of SSc patients develop interstitial lung disease (SSc-ILD), with 30-35% of related deaths attributed to it. Even though men are less likely to develop systemic sclerosis, they have a higher incidence of SSc-ILD than women, and they tend to develop it at a younger age with a higher mortality rate. Sex differences in protein expression in the blood of patients with SSc-ILD have not been reported to date. We aimed to identify sex differences in serum protein expression between men and women with SSc-ILD.</p><p><strong>Methods: </strong>Serum specimens of patients with SSc-ILD underwent dual mass spectrometry (LC-MS/MS) analysis. The association between protein biomarkers and sex was assessed through logistic regression. Time to event analysis was performed to determine any differences in the time to FVC decline of > 5% and the proportion of subjects who experienced FVC decline of > 5% by sex over the total period of observation. The association between biomarkers and sex was assessed through logistic regression. For proteins that were dichotomized, chi-squared testing was used. Multivariable regression models adjusting for meaningful clinical variables were also performed.</p><p><strong>Results: </strong>The cohort consisted of 211 subjects, 162 women and 47 men with a median follow-up of 3.52 years. No significant sex differences were found in the time to FVC decline of > 5% or > 10%. Among the 704 proteins identified, forty differed significantly between sexes. After adjusting for multiple testing, Autotaxin remained significantly higher in women. Autotaxin, known to activate lysophosphatidic acid and promote fibrosis, suggests a potential role in modulating fibrotic processes in SSc-ILD.</p><p><strong>Conclusions: </strong>This study is the first to report sex-specific serum protein differences in patients with SSc-ILD, with Autotaxin remaining significantly different after adjusting for multiple testing. These proteins could influence disease progression and treatment response and underscore the importance of personalized therapeutic strategies and further research into sex-related molecular pathways in SSc-ILD.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":9148,"journal":{"name":"BMC Pulmonary Medicine","volume":"25 1","pages":"16"},"PeriodicalIF":2.6,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142977088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effects of myrtenol against paraquat-induced toxicity in rats.","authors":"Fatemeh Amin, Hosein Basirat, Najmeh Parvaz, Morteza Khademalhosseini, Elham Hakimizadeh, Iman Fatemi","doi":"10.1186/s12890-025-03484-5","DOIUrl":"10.1186/s12890-025-03484-5","url":null,"abstract":"<p><strong>Background: </strong>Paraquat (PQ) is a widely used pesticide, can cause severe intoxication and respiratory failure. Myrtenol (Mrl), an essential oil derived in various plants, exhibits several biological properties, including anti-inflammatory and antioxidant activities. This study aims to investigate the protective potential of Mrl against oxidative stress and inflammation caused by PQ exposure.</p><p><strong>Methods: </strong>Twenty-five Wistar albino rats were divided into the following groups (n = 5 in each group): a control group (treated by dimethyl sulfoxide (DMSO)), a PQ group (exposed to 54 mg/m³ aerosol PQ), and two treatment groups that were exposed to PQ aerosol and administered oral Mrl at doses of 25 mg/kg/day and 50 mg/kg/day, respectively. The final group was exposed to PQ aerosol and treated with oral dexamethasone at a dose of 0.03 mg/kg/day. Various hematological, oxidative, inflammatory, and pathological indices were measured at the conclusion of the treatment period.</p><p><strong>Results: </strong>PQ decreases the levels or activities of superoxide dismutase (SOD), catalase (CAT), and Thiol, while increasing the levels or activities of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and malondialdehyde (MDA). Mrl restored activites of SOD, and CAT, as well as thiol levels to near-control values while reducing TNF-α, IL-6, and MDA levels. Pathological studies further confirmed the therapeutic effects of Mrl.</p><p><strong>Conclusion: </strong>The results of this study demonstrate the promising therapeutic effects of Mrl against inhaled PQ in rats.</p>","PeriodicalId":9148,"journal":{"name":"BMC Pulmonary Medicine","volume":"25 1","pages":"17"},"PeriodicalIF":2.6,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142977099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maximizing phonation: impact of inspiratory muscle strengthening on vocal durations and pitch range.","authors":"Coşkun Yilmaz, Özgür Bostanci, Özgür Eken, Rania Alkahtani, Monira I Aldhahi","doi":"10.1186/s12890-024-03471-2","DOIUrl":"10.1186/s12890-024-03471-2","url":null,"abstract":"<p><strong>Background: </strong>This study investigated the acute effects of inspiratory muscle warm-up (IWU) on vocal performance in singers. Proper vocal and respiratory warm-up can enhance vocal range, quality, and endurance. The aim was to determine whether IWU improves maximum phonation time and pitch range, contributing to better voice production efficiency (vocal efficiency) and reduced fatigue.</p><p><strong>Materials and methods: </strong>Singers were selected from the Samsun State Opera and the Ballet Directorate (n = 16). This cross-sectional study aimed to investigate the acute effects. The singers in the control group (SC = 8) performed only one session of routine voice warm-up, and the experimental group (SE = 8) conducted an inspiratory muscle warm-up (IWU) of 2 sets, 30 times/set at 40% maximal inspiratory pressure (MIP) in addition to routine voice warm-up. Subsequently, All participants were then required to perform pre- and post- pulmonary function tests, maximal inspiratory pressure (MIP) and maximal expiratory pressure (MEP), and voice recordings (note high pitch, note low pitch, high pitch durations and low pitch durations sustained with one breath, and maximum phonation duration).</p><p><strong>Results: </strong>All pulmonary function and muscle strength parameters improved in the SE group, with the highest increases in MIP (22.9%) and MEP (14.7%). No significant improvements were noted in the SC group (p > 0.05). The Borg Rating of Perceived Exertion showed that the SE group experienced less difficulty with their vocal performance after IWU (-11.6%, p = 0.006), while no significant change was observed in the SC group (p = 0.316). Both warm-up methods used in the study significantly affected the frequencies of high-pitch sounds (SE = 17.8%, SC = 10.9%, p = 0.003); however, the frequency of low-pitch sounds was not significantly affected (p = 0.437). IWU significantly affected the high-pitched note duration (p < 0.001; 32.17%), low-pitched note duration (p < 0.001; 27.11%), and maximum phonation time (p < 0.001; 21%), while routine voice warm-up did not significantly affect any parameter (p > 0.05).</p><p><strong>Conclusions: </strong>The combination of IWU with the general body and voice warm-up protocol can acutely improve vocal performance in terms of maximum phonation time, phonation times of the highest and lowest pitched sounds in a single breath, and vocal range levels.</p>","PeriodicalId":9148,"journal":{"name":"BMC Pulmonary Medicine","volume":"25 1","pages":"15"},"PeriodicalIF":2.6,"publicationDate":"2025-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725213/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142968826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological characterization and clinical significance of cuproptosis-related genes in lung adenocarcinoma.","authors":"Meilin Li, Yu Tan, Zhixin Li, Lingfeng Min","doi":"10.1186/s12890-025-03477-4","DOIUrl":"10.1186/s12890-025-03477-4","url":null,"abstract":"<p><strong>Background: </strong>Lung cancer has high morbidity and mortality rates, which results in a poor prognosis. Cuproptosis is a novel cell death mechanism. The aim of this study was to examine the biological characteristics and clinical significance of genes associated with cuproptosis in lung adenocarcinoma (LUAD), and to understand the molecular mechanisms underlying the occurrence and progression of LUAD.</p><p><strong>Methods: </strong>We targeted 10 cuproptosis-related genes from previous studies and used the datasets from GEO and TCGA databases to identify differential genes related to cuproptosis; then the data were analyzed by R package, Cytoscape, TISDB, cBioPortal, STRING, CancerSEA, and Disgenet; and finally, the data were detected by immunohistochemistry validation was performed.</p><p><strong>Results: </strong>CDKN2A and MTF1 were cuproptosis-associated LUAD differential genes and were differentially expressed in immune subtypes. The expression of CDKN2A and MTF1 showed correlation with multiple functional states of LUAD.CDKN2A was negatively correlated with LUAD survival prognosis.</p><p><strong>Conclusion: </strong>CDKN2A and MTF1 were correlated with the diagnosis of LUAD, and CDKN2A was negatively correlated with the survival and prognosis of LUAD. CDKN2A has the potential to contribute to the early diagnosis and prognosis analysis of LUAD.</p>","PeriodicalId":9148,"journal":{"name":"BMC Pulmonary Medicine","volume":"25 1","pages":"13"},"PeriodicalIF":2.6,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725195/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive analysis pinpoints CCNA2 as a prognostic and immunological biomarker in non-small cell lung cancer.","authors":"Liming Zhang, Shaoqiang Wang, Lina Wang","doi":"10.1186/s12890-025-03490-7","DOIUrl":"10.1186/s12890-025-03490-7","url":null,"abstract":"<p><strong>Background: </strong>Lung cancer is a leading cause of morbidity and mortality globally. Despite advances in targeted and immunotherapies, overall survival (OS) rates remain suboptimal. Cyclin-A2 (CCNA2), known for its upregulation in various tumors and role in tumorigenesis, has an undefined function in non-small cell lung cancer (NSCLC).</p><p><strong>Methods: </strong>We analyzed three microarray datasets from the Gene Expression Omnibus (GEO) repository to identify differentially expressed genes. Using STRING, we constructed a protein-protein interaction (PPI) network to pinpoint hub genes. The expression and prognostic relevance of CCNA2 were validated using GEPIA and the Kaplan-Meier plotter. Clinicopathological correlations were assessed via the Human Protein Atlas (HPA) and UALCAN databases. qRT-PCR and immunohistochemistry (IHC) were performed to validate CCNA2 mRNA and protein levels. Loss-of-function assays in lung cancer cell lines evaluated the biological role of CCNA2. Immune infiltration and single-cell sequencing were also explored.</p><p><strong>Results: </strong>Analysis of GSE18842, GSE101929, and GSE116959 datasets identified 321 upregulated and 623 downregulated genes in NSCLC. CCNA2 was confirmed to be highly expressed in NSCLC through qRT-PCR and IHC, with overexpression correlating with advanced pathological stages and lymph node metastasis. The area under the curve (AUC) of CCNA2 indicating high diagnostic accuracy. Immune infiltration and single-cell sequencing revealed that CCNA2 expression was significantly associated with immune cell infiltration, particularly in Tprolif cells.</p><p><strong>Conclusion: </strong>CCNA2 is upregulated in NSCLC and shows significant correlation with clinicopathological characteristics. Our findings suggest that CCNA2 may serve as a promising biomarker for both the prognosis and diagnosis of NSCLC.</p>","PeriodicalId":9148,"journal":{"name":"BMC Pulmonary Medicine","volume":"25 1","pages":"14"},"PeriodicalIF":2.6,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11725219/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metagenomic analysis identifying a polymicrobial pulmonary infection in a non-HIV immunocompromised patient: a case report.","authors":"Jing Duan, Jie Ding, Yupeng Wei, Yingxin Zhang, Zhiqing You, Ding Li, Chao Chen","doi":"10.1186/s12890-024-03473-0","DOIUrl":"10.1186/s12890-024-03473-0","url":null,"abstract":"<p><strong>Background: </strong>Polymicrobial pulmonary infections, common in immunocompromised patients, often manifest more severe symptoms than monomicrobial infections. Clinical diagnosis delays may lead to mortality, emphasizing the importance of fast and accurate diagnosis for these patients. Metagenomic next-generation sequencing (mNGS), as an unbiased method capable of detecting all microbes, is a valuable tool to identify pathogens, particularly in cases where infections are difficult to diagnosis using conventional methods.</p><p><strong>Case presentation: </strong>A 50-year-old male patient was admitted due to cough, expectoration and dyspnea. CT scan revealed diffuse inflammatory and cavernous lung lesion, and blood examination suggested a polymicrobial infection. However, no etiology was found by routine examination. mNGS of bronchoalveolar lavage fluid(BALF)simultaneously detected the presence of Pneumocystis jirovecii (P.jirovecii), Aspergillus fumigates (A.fumigates), Nocardia farcinica (N.farcinica), Salmonella enterica subsp. enterica (S.enterica subsp. enterica), and cytomegalovirus (CMV). The patient was successfully treated with compound sulfamethoxazole (SMZ-TMP), cefoperazone/sulbactam (SCF), moxifloxacin (MXF), voriconazole (VCZ), and ganciclovir. The patient recovered after two weeks of anti-infection therapy and maintained good health at a six-month follow-up.</p><p><strong>Conclusion: </strong>For immunocompromised patients with multiple infections and atypical symptoms, mNGS emerged as a reliable approach to pathogen detection and guiding antibiotic therapy.</p>","PeriodicalId":9148,"journal":{"name":"BMC Pulmonary Medicine","volume":"25 1","pages":"12"},"PeriodicalIF":2.6,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11720941/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142944663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lung ultrasound for assessing disease progression in UIP and NSIP: a comparative study with HRCT and PFT/DLCO.","authors":"Nasrollahzadeh Milad, Idani Esmaeil, Abedini Atefeh, Malekshoaar Mehran, Sadeghi Mohsen, Kasravi Maryam, Tofighi Rozhin, Roostaei Ghazal, Kiani Arda","doi":"10.1186/s12890-024-03433-8","DOIUrl":"10.1186/s12890-024-03433-8","url":null,"abstract":"<p><strong>Background: </strong>This study aims to compare Lung Ultrasound (LUS) findings with High-Resolution Computerized Tomography (HRCT) and Pulmonary Function Tests (PFTs) to detect the severity of lung involvement in patients with Usual Interstitial Pneumonia (UIP) and Non-Specific Interstitial Pneumonia (NSIP).</p><p><strong>Methods: </strong>A cross-sectional study was conducted on 35 UIP and 30 NSIP patients at a referral hospital. All patients underwent LUS, HRCT, and PFT. LUS findings such as B-lines, pleural fragmentation, and pleural thickening were compared with HRCT-based lung involvement and PFT parameters.</p><p><strong>Results: </strong>In UIP patients, B-lines > 18 and pleural fragmentation significantly differentiated between < 50% and > 50% HRCT involvement. A logistic regression model showed that B-lines > 18 (OR = 39, p = 0.04) and pleural fragmentation (OR = 22, p = 0.037) independently predicted > 50% HRCT involvement. ROC analysis of the model revealed 84.2% sensitivity and 84.5% specificity. Furthermore, the crude number of B-lines (OR = 1.2, p = 0.038) and > 50% HRCT involvement (OR = 9.5, p = 0.045) independently predicted severe DLCO impairment, with a sensitivity of 94.7% and specificity of 84.5%. Linear regression showed that each additional B-line was associated with a 0.4% decrease in DLCO (Beta = -0.377, p = 0.043), independent of patient diagnosis. In NSIP patients, no significant correlation was observed between LUS findings and > 50% HRCT involvement (p > 0.05), though B-line numbers and pleural thickening increased in cases with severe DLCO impairment (p < 0.05).</p><p><strong>Conclusions: </strong>LUS shows promise as a sensitive, radiation-free alternative to HRCT in monitoring the severity of UIP. It is particularly valuable in predicting the extent of lung involvement and severe DLCO impairment in UIP patients but has limited application in NSIP.</p>","PeriodicalId":9148,"journal":{"name":"BMC Pulmonary Medicine","volume":"25 1","pages":"11"},"PeriodicalIF":2.6,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11716402/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142944716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Iatrogenic intramural esophageal hematoma during EUS-B-FNA procedure.","authors":"João Oliveira Rodrigues, Paulo Matos, Luís Vaz Rodrigues, Michele de Santis, Lourdes Barradas","doi":"10.1186/s12890-024-03470-3","DOIUrl":"10.1186/s12890-024-03470-3","url":null,"abstract":"<p><strong>Background: </strong>Esophageal ultrasound with bronchoscope fine needle aspiration (EUS-B-FNA) is a valuable tool for the diagnosis and staging of lung cancer, complementing endobronchial lung ultrasound (EBUS). While generally considered safe, there is a notable lack of comprehensive knowledge within the interventional pulmonology community regarding potential complications.</p><p><strong>Case presentation: </strong>We present a case involving a 66-year-old male with squamous cell lung carcinoma undergoing mediastinal staging. A systematic mediastinal assessment through EBUS confirmed the presence of enlarged lymph nodes at 4 L with limited access to puncture. Complementary EUS-B widened the visualization and access to station 4 L and after excluding nearby vessels, a single puncture was performed. Unexpectedly, an iatrogenic esophageal hematoma was promptly noted at the puncture site. The procedure was immediately interrupted, and subsequent workup confirmed the hematoma without active bleeding. Conservative management, including upper endoscopy and clip sealing, resulted in the patient's asymptomatic recovery.</p><p><strong>Conclusion: </strong>This case underscores the importance of recognizing and managing complications associated with EUS-B-FNA, emphasizing the need for heightened awareness and education in the interventional pulmonology community. Despite being infrequently discussed in medical literature, EUS-B-FNA can give rise to both immediate and delayed complications, warranting increased vigilance during its practice.</p>","PeriodicalId":9148,"journal":{"name":"BMC Pulmonary Medicine","volume":"25 1","pages":"10"},"PeriodicalIF":2.6,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11715552/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142944712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}