BMC Pulmonary Medicine最新文献

{"title":"Chest mobility, cough strength, muscle strength, physical activity, and quality of life in parkinson's patients who had COVID-19.","authors":"Merve Şahin, Gülşah Barğı, Yasemin Ünal, Özlem Çinar Özdemir","doi":"10.1186/s12890-025-03938-w","DOIUrl":"10.1186/s12890-025-03938-w","url":null,"abstract":"<p><strong>Background: </strong>Some symptoms of COVID-19 may persist for a long time in individuals who had COVID-19. Since effects of COVID-19 on chest mobility, cough strength, grip strength, physical activity, and quality of life in Parkinson's patients are still unclear, current study aimed to investigate this issue.</p><p><strong>Methods: </strong>Parkinson's patients with and without a history of COVID-19 (n = 17, 66.65 ± 12.01 years versus n = 21, 68.71 ± 10.35 years) were included in this study. Cognitive functions (Mini-Mental State Examination), severity and disability status of Parkinson's disease (Movement Disorder Society-Unified Parkinson's Disease Rating Scale (MDS-UPDRS), Modified Hoehn and Yahr Scale (MHYS)), chest mobility (chest circumference measurement with a tape measure), cough strength (a PEFmeter), hand grip strength (a hand-dynamometer), physical activity level (a pedometer) and quality of life (Parkinson's Disease Questionnaire-39 (PDQ-39)) were evaluated in all individuals.</p><p><strong>Results: </strong>Parkinson's patients with COVID-19 history had lower subcostal chest circumference measurement difference, grip strength and step count measured on 1st day, and higher MDS-UPDRS part I and PDQ-39 scores than patients without COVID-19 history (p < 0.05). Cognitive functions, MHYS, other parts of MDS-UPDRS, axillar and epigastric chest circumference measurement differences, cough strength and average step counts values were similar between groups (p > 0.05).</p><p><strong>Conclusions: </strong>Disease severity due to non-motor symptoms, abdominal respiration, peripheral muscle strength, daily step count and quality of life worsened in Parkinson's patients with COVID-19 history compared to those without COVID-19 history and these parameters should be therefore monitored regularly. Chest respiration and cough strength were preserved in these individuals.</p>","PeriodicalId":9148,"journal":{"name":"BMC Pulmonary Medicine","volume":"25 1","pages":"467"},"PeriodicalIF":2.8,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12523216/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145291019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of metabolic syndrome and prognosis in elderly patients with community-acquired pneumonia.","authors":"Xiaofeng Tang, Li Zhang, Ruijuan Wu, Jie Liu, Yongfeng Cheng","doi":"10.1186/s12890-025-03914-4","DOIUrl":"10.1186/s12890-025-03914-4","url":null,"abstract":"<p><strong>Background: </strong>This study aimed to evaluate the association between metabolic syndrome (MetS) and clinical outcomes in elderly patients hospitalized with community-acquired pneumonia (CAP).</p><p><strong>Methods: </strong>A retrospective cohort study was conducted on 712 elderly patients (aged ≥ 65 years) admitted with CAP to a tertiary hospital between 2018 and 2022. Patients were stratified into two groups based on MetS status (MetS group vs. non-MetS group). The primary outcome was 90-day all-cause mortality. Propensity score matching (PSM) was applied to balance baseline characteristics. Multivariate Cox regression and logistic regression analyses were performed to assess associations, adjusting for confounders.</p><p><strong>Results: </strong>After propensity score matching, 336 patients (168 MetS and 168 non-MetS) were included in the final analysis. The MetS group had a significantly higher 90-day mortality rate compared to the non-MetS group (29.8% vs. 11.9%, p < 0.01). Multivariate Cox regression analysis demonstrated that MetS was independently associated with a 1.98-fold increased risk of mortality (95% CI: 1.08-3.63; p = 0.03). A clear dose-response relationship was observed, with each additional MetS component increasing mortality risk by 1.46-fold (95% CI: 1.11-2.16; p < 0.01).</p><p><strong>Conclusion: </strong>MetS is an independent predictor of worse short-term outcomes in elderly CAP patients, including higher mortality and longer hospital stays. Early recognition and management of MetS may improve prognosis in this high-risk population.</p>","PeriodicalId":9148,"journal":{"name":"BMC Pulmonary Medicine","volume":"25 1","pages":"469"},"PeriodicalIF":2.8,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12522349/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145291069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic impact of muscle mass in idiopathic interstitial pneumonia: analysis of idiopathic pulmonary fibrosis and other idiopathic interstitial pneumonias.","authors":"Hirotaka Hagiwara, Tomotsugu Takano, Hiroaki Ogata, Kazuya Tsubouchi, Katsuyuki Ichiki, Shohei Takata, Hiroshi Ishii, Yasuhiko Kitasato, Yoshiaki Zaizen, Kazuhiro Yatera, Masayuki Kawasaki, Masaki Fujita, Makoto Yoshida, Takashige Maeyama, Ayano Mashimoto, Kazuto Furuyama, Ryo Torii, Kunihiro Suzuki, Yuichi Mizuta, Kazunori Tobino, Eiji Harada, Fumiaki Kiyomi, Hidetake Yabuuchi, Yoichi Nakanishi, Isamu Okamoto","doi":"10.1186/s12890-025-03942-0","DOIUrl":"10.1186/s12890-025-03942-0","url":null,"abstract":"","PeriodicalId":9148,"journal":{"name":"BMC Pulmonary Medicine","volume":"25 1","pages":"468"},"PeriodicalIF":2.8,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12522827/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145291090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical, radiological, and laboratory assessment of pulmonary eosinophilia syndrome: a case control study in Saudi Arabia.","authors":"Mona Ibrahim Ahmed, Salah A M Ali, Randa I Ahmed","doi":"10.1186/s12890-025-03860-1","DOIUrl":"10.1186/s12890-025-03860-1","url":null,"abstract":"<p><strong>Background: </strong>Pulmonary eosinophilic syndromes (PES) are a heterogeneous group of respiratory disorders characterized by eosinophilic infiltration of lung tissues and/or elevated eosinophil counts in peripheral blood. Accurate diagnosis and understanding of PES are crucial for effective management, especially in underrepresented regions such as Saudi Arabia.</p><p><strong>Objectives: </strong>To evaluate the clinical, radiological, and biomarker profiles of PES in a Saudi Arabian cohort and to identify factors associated with disease presentation and severity.</p><p><strong>Methods: </strong>This retrospective case-control study included 95 patients diagnosed with PES at Mouwasat Hospital, Jubail, Eastern Province, Saudi Arabia, from January 2023 to January 2024. Clinical data, laboratory findings (including eosinophil counts, IgE, and FeNO levels), pulmonary function tests, and radiological imaging were analyzed. Statistical analysis was performed using SPSS, with descriptive and inferential methods to assess associations.</p><p><strong>Results: </strong>The cohort had a mean age of 40.5 ± 12.9 years, with a majority being male (65.3%) and Saudi nationals (87%). Elevated eosinophil counts and FeNO levels were significantly associated with PES (p < 0.05). Patients exhibited characteristic radiological patterns such as ground-glass opacities and consolidations. Pulmonary function tests showed reduced FEV₁ and small airway impairment in cases versus controls (p = 0.027). The study observed strong correlations between eosinophilia, airway obstruction, and higher BMI. Smoking was associated with increased symptom severity.</p><p><strong>Conclusions: </strong>The study underscores the distinct clinical, radiological, and biomarker features of PES in the included population. Integrating multi-modal assessments can improve diagnostic accuracy. These findings emphasize the importance of standardized pathways for PES diagnosis, especially in regions with potential underdiagnosis, and highlight the need for increased awareness among healthcare professionals.</p>","PeriodicalId":9148,"journal":{"name":"BMC Pulmonary Medicine","volume":"25 1","pages":"466"},"PeriodicalIF":2.8,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12515387/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Small airway lesions appear with the course of IPF and relate to the severity of pulmonary fibrosis progression.","authors":"Xiaoyan Wang, Ling Zhao, Dingyun Song, Xinran Zhang, Min Liu, Huaping Dai","doi":"10.1186/s12890-025-03939-9","DOIUrl":"10.1186/s12890-025-03939-9","url":null,"abstract":"<p><strong>Aim: </strong>Idiopathic Pulmonary Fibrosis (IPF) has long been considered a disease primarily involving the lung interstitium, with relative sparing of the airways. This study aimed to investigate the presence and characteristics of small airway abnormalities in patients with IPF.</p><p><strong>Methods: </strong>We analyzed 137 patients with IPF and 84 controls from a prospective, multicenter cohort (trial registration: NCT03666234). IPF patients were stratified by fibrosis severity on CT and clinical disease severity scores. Small airway function was assessed using predicted maximal expiratory flow at 25% (MEF25%), 50% (MEF50%), and 25-75% of forced vital capacity (MEF25-75%). Airway segmentation and quantitative analysis of airway number, length, and volume were performed using FACT-Digital Lung™ software. Group differences were analyzed using SPSS (version 26).</p><p><strong>Results: </strong>The IPF group comprised 114 men and 23 women, with a mean age of 64.5 ± 10.7 years. MEF50%, MEF25%, and MEF25-75% were significantly lower in IPF patients than in controls across all severity stages. Conversely, the total number, length, and volume of segmented bronchi were higher in IPF patients, with the most pronounced differences observed in bronchi at generations 9-14. Small airway abnormalities were evident even in patients with mild fibrosis.</p><p><strong>Conclusion: </strong>Small airway abnormalities, including increased airway counts and functional impairment, are detectable in early-stage IPF and persist throughout disease progression, suggesting that small airway involvement is a fundamental feature of IPF.</p>","PeriodicalId":9148,"journal":{"name":"BMC Pulmonary Medicine","volume":"25 1","pages":"465"},"PeriodicalIF":2.8,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12512905/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Solitary pulmonary chondroma in a 59-year-old Caucasian woman: a case report : Authorship.","authors":"Brenda Gomis, Anne Durlach, Alexandre Vivien, Maxime Dewolf, Gonzague Delepine, Gaëtan Deslee, Julien Ancel","doi":"10.1186/s12890-025-03905-5","DOIUrl":"10.1186/s12890-025-03905-5","url":null,"abstract":"<p><strong>Background: </strong>Pulmonary chondroma is a rare benign tumor made of chondrocytes, typically presenting radiologically as solitary or multiple pulmonary nodules. It can also be encountered in the Carney's triad, which associates pulmonary chondroma, gastro-intestinal stromal tumors (GIST) and adrenal paraganglioma. We report a rare case of pulmonary chondroma presenting with atypical radiological features.</p><p><strong>Case presentation: </strong>A 59-year-old woman was referred for chronic dyspnea and a smoking history of 40 pack-years quitted 9 years ago. Pulmonary function tests revealed a moderate chronic obstructive pulmonary disease. A thoraco-abdominal CT-scan revealed a 2-cm non-calcified nodule of the left lower lobe with a low [18 F] fluoro-2-deoxy-D-glucose (18 F-FDG) uptake (SUV max: 1,1). Fiberoptic bronchoscopy was normal. Surgical resection of the nodule was performed revealing a benign tumor made of mature cartilage allowing the diagnosis of a pulmonary chondroma. No evidence of GIST or adrenal paraganglioma was identified. No recurrence was observed after 24 months of follow-up.</p><p><strong>Conclusion: </strong>The occurrence of pulmonary chondroma is uncommon. Its potential association with GIST or paragangliomas warrants careful, long-term surveillance.</p>","PeriodicalId":9148,"journal":{"name":"BMC Pulmonary Medicine","volume":"25 1","pages":"464"},"PeriodicalIF":2.8,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12512281/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sex-based disparities in asthma prevalence in Sierra Leone.","authors":"Augustus Osborne","doi":"10.1186/s12890-025-03829-0","DOIUrl":"10.1186/s12890-025-03829-0","url":null,"abstract":"<p><strong>Background: </strong>Asthma is a major public health concern globally, with notable disparities in prevalence across regions and demographic groups. In Sierra Leone, a country with limited healthcare infrastructure, asthma is a challenge. Sex-based disparities in asthma prevalence have been documented globally, yet their impact in Sierra Leone remains poorly understood. This study aims to provide age-standardized estimates of asthma prevalence in 2000, 2005, 2010, 2015, and 2019, focusing on trends and inequalities by sex.</p><p><strong>Methods: </strong>The study utilized data from the World Health Organization equity data repository to estimate age-standardized asthma prevalence in Sierra Leone. Sex-disaggregated analyses was conducted to assess disparities in prevalence rates. Inequality measures, including absolute difference (D), prevalence ratio (R), population attributable fraction (PAF), and population attributable risk (PAR), were calculated to quantify sex-related disparities in asthma prevalence.</p><p><strong>Results: </strong>In 2000, the age-standardized asthma prevalence in Sierra Leone was 2,807.2 per 100,000, increasing slightly to 2,855.5 per 100,000 in 2005, followed by a decline to 2,682.7 per 100,000 in 2010. Prevalence rose again to 2,709.8 per 100,000 in 2015 and reached 2,768.1 per 100,000 in 2019. Across all years, females consistently had higher asthma prevalence rates than males. In 2019, female asthma prevalence was 3,104.7 per 100,000 compared to 2,427.8 per 100,000 for males, with an absolute difference of 677.0 per 100,000 and a prevalence ratio of 1.3. Inequality measures revealed a gap over time, with negative PAF values (-5.9% in 2000 to -12.3% in 2019) and negative PAR values (-164.7 per 100,000 in 2000 to -340.3 per 100,000 in 2019).</p><p><strong>Conclusion: </strong>The study points out the burden of asthma in Sierra Leone and the sex-based disparities in prevalence. The disproportionate burden among females underscores the need for interventions to address biological, behavioural, and environmental factors contributing to these inequalities. Policymakers should prioritize sex-disaggregated surveillance and implement sex-sensitive strategies to reduce asthma prevalence. Future research should investigate the underlying causes of sex-based disparities, including the role of environmental exposures and healthcare access, to inform more effective and equitable asthma control measures in Sierra Leone.</p>","PeriodicalId":9148,"journal":{"name":"BMC Pulmonary Medicine","volume":"25 1","pages":"463"},"PeriodicalIF":2.8,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12513104/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145257465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conditioned media from adipose-derived mesenchymal stem cells improves movento induced lung damage in male rats.","authors":"Iman Zangiabadi, Mohammad Amin Rajizadeh, Majid Askaripour, Mohammad Mehdi Bagheri, Elham Jafari, Soodeh Rajabi","doi":"10.1186/s12890-025-03940-2","DOIUrl":"10.1186/s12890-025-03940-2","url":null,"abstract":"<p><p>Insecticides are chemicals agents used to kill insects to increase crop growth. But they can be toxic to humans and wildlife. Movento is an insecticide which is highly toxic to humans and animals by oral, dermal, and inhalation routes. This research is to assess the pulmonary impacts of this insecticide, which have not been explored in previous research. Wistar rats were adopted to four groups. 1- Control (CTL), 2- Movento (M), 3- M in conjunction with Basal medium (BM), 4- M in conjunction with Conditioned medium (CM). For evaluation of the degree of injury and fibrosis, the Hematoxylin and Eosin (H&E) and Masson's trichrome stainings were done respectively. Oxidative stress markers and inflammatory mediators were analyzed utilizing appropriate assays. In rats administered Movento's insecticide, antioxidant markers diminished in comparison to the control group; however, curation with CM markedly elevated them relative to the M and M + BM groups. Movento elevated histological score, fibrosis, MDA, TNF-α, and IL-6 compared to the control group, whereas CM remarkably diminished these parameters in relation to the M and M + BM groups. Conditioned media derived from mesenchymal stem cells (CM-MSC) may function as a therapeutic intervention for lung illnesses induced by movento in rat lungs. It may also serve as a potential treatment of alleviating lung injuries in farmers who are exposed to agricultural pesticides.</p>","PeriodicalId":9148,"journal":{"name":"BMC Pulmonary Medicine","volume":"25 1","pages":"461"},"PeriodicalIF":2.8,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12505815/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potential of non-invasive remote dielectric sensing in predicting short-term prognosis of patients with pulmonary hypertension.","authors":"Dandan Chen, Dan Tian, Qi Jin, Lei Zhang, Xiaochun Zhang, Mingfei Li, Wenzhi Pan, Qianzhou Lv, Daxin Zhou, Junbo Ge, Lihua Guan","doi":"10.1186/s12890-025-03922-4","DOIUrl":"10.1186/s12890-025-03922-4","url":null,"abstract":"<p><strong>Background: </strong>Pulmonary hypertension (PH) is exhibits a profound pathophysiological association with right heart failure. The non-invasive Remote Dielectric Sensing (ReDS™) technology demonstrates exceptional sensitivity in identifying pathological states, ranging from mild cardiac compensation to severe pulmonary edema. This study aims to develop a PH-ReDS predictive model using ReDS™ technology, leveraging real-world clinical data to evaluate the risk of all-cause mortality or clinical deterioration in patients with PH.</p><p><strong>Methods: </strong>We conducted a prospective, convenience-sampled observational pilot study involving adult patients with PH admitted to or followed as outpatients in the cardiology department of our institution. ReDS™ Pro was used to measure dielectric sensing values. Clinical data were extracted from the electronic medical record system of our hospital. Patients were followed up for 6 months post-discharge. The primary endpoints defined as all-cause mortality or PH-related clinical deterioration.</p><p><strong>Results: </strong>A total of 202 patients were included, with 24.8% (n = 50) being male. Lung fluid was significantly correlated with left atrial diameter (r = 0.338, p < 0.001), left ventricular ejection fraction (r = -0.256, p < 0.001), and mean right atrial pressure (mRAP; r = 0.219, p = 0.007). Multivariate Cox regression analysis identified lung fluid level, renal insufficiency, and NT-proBNP as significant independent risk factors for PH deterioration. ROC analysis revealed that lung fluid and mRAP effectively discriminated between patients with and without short-term clinical deterioration related to PH, with optimal cut-off values of 30.5% for lung fluid and 6.5 mmHg for mRAP. The Combined PH-ReDS models incorporating lung fluid and mRAP significantly enhanced predictive accuracy, achieving the highest AUC value of 0.733 (95% CI: 0.551-0.916, p = 0.010). Patients with elevated lung fluid levels were found to have a significantly higher risk of short-term clinical deterioration related to PH, with a hazard ratio (HR) of 3.670 (95% CI: 1.274-10.571, p = 0.016).</p><p><strong>Conclusions: </strong>The PH-ReDS model demonstrated robust predictive efficacy for all-cause mortality or clinical deterioration in patients with PH. Notably, elevated lung fluid levels were significantly associated with an increased risk of short-term PH-related clinical deterioration, suggesting its potential as an early intervention indicator in clinical practice.</p><p><strong>Trial registration: </strong>This trial was retrospectively registered with Clinical Trials. gov on 2025/04/24. The registration number was NCT06942871 .</p>","PeriodicalId":9148,"journal":{"name":"BMC Pulmonary Medicine","volume":"25 1","pages":"460"},"PeriodicalIF":2.8,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12505569/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LncNFYC-AS1 ameliorates Mycoplasma pneumoniae pneumonia via regulating miR-1323.","authors":"Cai Chen, Jianping Hu, Mao Guo, Li Li, Qihong Yang","doi":"10.1186/s12890-025-03926-0","DOIUrl":"10.1186/s12890-025-03926-0","url":null,"abstract":"<p><strong>Background: </strong>Mycoplasma pneumoniae pneumonia (MPP) is a common respiratory infection, yet its molecular mechanisms remain incompletely understood. Long non-coding RNAs (lncRNAs) play critical roles in various diseases, but their regulatory functions in MPP require further investigation. This study aimed to investigate the expression profile and clinical significance of NFYC-AS1 in MPP and analyze its interaction with miR-1323 in modulating cellular functions.</p><p><strong>Methods: </strong>In vitro cell culture methods were used to establish MPP cell models, and A549 cells were transfected to introduce or inhibit lncRNAs and miRNAs. Real-time quantitative PCR was used to detect the expression levels of NFYC-AS1 and miR-1323. The direct binding relationship between NFYC-AS1 and miR-1323 was verified using a luciferase reporter gene assay. Elisa was used to quantify the levels of cytokines TNF-α, IL-1β, and IL-6. CCK-8 assay was employed to assess cell growth capacity. Transwell analysis was used to study cell migration. Cell apoptosis rates were detected by flow cytometry. Finally, statistical analysis methods were used to process and interpret the data.</p><p><strong>Results: </strong>NFYC-AS1 was significantly upregulated in patients and significantly associated with MPP risk, showing a negative correlation with miR-1323. Mechanistically, NFYC-AS1 directly targeted and suppressed miR-1323. In MP-infected A549 cells, NFYC-AS1 knockdown reduced inflammatory cytokines (TNF-α/IL-1β/IL-6), enhanced proliferation/migration, and inhibited apoptosis-effects abolished by miR-1323 inhibition.</p><p><strong>Conclusion: </strong>NFYC-AS1 is highly expressed in MPP and regulates inflammatory responses, cell proliferation, migration, and apoptosis by targeting miR-1323. The NFYC-AS1/miR-1323 axis may serve as a potential therapeutic target and show preliminary discriminatory value for MPP.</p>","PeriodicalId":9148,"journal":{"name":"BMC Pulmonary Medicine","volume":"25 1","pages":"457"},"PeriodicalIF":2.8,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12505855/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}