BMC Complementary Medicine and Therapies最新文献

{"title":"Computational development of mushroom-6-glucan/paclitaxel as a synergistic complementary medicine for breast cancer therapy.","authors":"Nehal M El-Deeb, Omar M Ibrahim, Ayman M Kamel, Ahmed I Gomaa, Ahmed M Kenawy","doi":"10.1186/s12906-025-04772-7","DOIUrl":"10.1186/s12906-025-04772-7","url":null,"abstract":"<p><strong>Background: </strong>Breast cancer is chemo-resistant and highly metastatic, often resulting in patient mortality. One of the primary factors contributing to the metastasis and chemotherapy resistance is the presence of cancer stem-like cells. We posited that the natural polysaccharide known as 6-glucans, derived from Pleurotus ostreatus, could effectively counteract the chemotherapy resistance associated with cancer stem-like cells in breast cancer.</p><p><strong>Methods: </strong>We computationally developed a specific dual combinatorial therapy involving 6-glucans and Paclitaxel (PTX) and tested on preclinical 3D mammosphere human tumor models representing receptor-positive and receptor-negative breast cancer. Using this preclinical 3D spheroid technology, we tested the anti-cancer properties of these predicted treatment combinations on mammospheres containing human breast cancer stem cells.</p><p><strong>Results: </strong>Among the 40 distinct combinations examined, computational prediction revealed that the addition of 2.0 mg/mL of 6-glucans to a low dose of 3.0 µg/mL PTX was the sole combination demonstrating a synergistic effect. This optimized synergistic combination therapy displayed a significant inhibitory impact on human cancer epithelial and stem cell migration, evasion, and colony formation. The inclusion of 6-glucans also augmented apoptosis in both breast cancer cells and stem cells, leading to a six-fold reduction in BrdU labeled cells and an increased arrest of cells in the sub-G0 phase. These effects were mediated through mitochondrial dysfunction and the downregulation of associated oncogenes.</p><p><strong>Conclusion: </strong>Our study revealed that the computationally predicted 6-glucans-based binary complementary medicine exhibited sequence- and concentration-dependent anticancer synergistic effects.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"58"},"PeriodicalIF":3.3,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11830196/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143424844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of mechanical traction on cervical radiculopathy: protocol for the TracCerv2 single-blind, randomised controlled trial.","authors":"Grégoire Cormier, Chloé Moreau, Emilie Scalisi, Lydie Pastor, Thomas Rulleau","doi":"10.1186/s12906-025-04801-5","DOIUrl":"10.1186/s12906-025-04801-5","url":null,"abstract":"<p><strong>Objectives: </strong>To evaluate the effect at 3 months of an intensive cervical traction protocol on disability in people with cervical radiculopathy and compare with placebo traction.</p><p><strong>Design: </strong>The trial is national, multi-centre, randomised, placebo-controlled and single-blinded. It began in March 2024 and will end in September 2027. Participants are allocated to receive mechanical cervical traction or placebo mechanical cervical traction.</p><p><strong>Setting: </strong>Seven hospitals in France.</p><p><strong>Participants: </strong>We will include 206 individuals with cervical radiculopathy diagnosed 3 to 12 months previously, hospitalised to undergo mechanical traction. Main inclusion criteria: age ≥ 18 years, Neck Disability Index ≥ 15/50 points and presence of ≥ 3 of 4 diagnostic signs of cervical radiculopathy.</p><p><strong>Interventions: </strong>All participants undergo 2 × 30 min of traction per day for 5 consecutive days. For mechanical cervical traction, the maximum weight is ≤ 12 kg and for placebo traction ≤ 600g.</p><p><strong>Main outcome measures: </strong>The primary outcome is disability (Neck Disability Index), secondary outcomes include pain related outcomes, medication consumption, surgery and days off work.</p><p><strong>Results: </strong>This study will provide a robust evaluation of the mid-term effectiveness of mechanical traction on disability in chronic cervical radiculopathy. The results will demonstrate whether a simple technique involving a short, intensive protocol reduces the duration of disability and pain.</p><p><strong>Conclusions: </strong>The availability of robust evidence supporting or refuting the use of cervical traction as part of the management of cervical radiculopathy will enable optimisation of treatment. The results could lead to the drafting of evidence-based recommendations regarding the use of mechanical traction to treat cervical radiculopathy.</p><p><strong>Clinial trial registration number: </strong>ClinicalTrials.gov (NCT05952167).</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"56"},"PeriodicalIF":3.3,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11829517/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143424864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro characterization of potential botanicals to reduce infection and improve the rate of wound healing in humans and canines.","authors":"Morgan Detering, Ashley Langland, Anabel Terry, Jeffrey Langland","doi":"10.1186/s12906-025-04777-2","DOIUrl":"10.1186/s12906-025-04777-2","url":null,"abstract":"<p><p>Wounds in both humans and canines are injuries that result in damage to the body's tissues. Wounds can range from minor superficial scratches to severe and deep injuries. Complications of wounds, both in humans and canines, can range from mild to severe. Proper wound care and timely treatment are essential to prevent complications and promote healing. One of the most common and concerning complications of wounds is infection. When the skin is broken, bacteria and other pathogens can enter the wound leading to an infection. In severe cases, infections can spread, causing systemic issues and even sepsis, which can be life-threatening. Some wounds may have difficulty healing properly, leading to delayed wound closure. This can occur due to various factors including poor blood supply to the wound site, certain medical conditions, age, and nutritional deficiencies. Early intervention and appropriate wound care are crucial in reducing the risk of complications. This study screened the ability of various botanical extracts (prepared in both low and high ethanol) to reduce the replication of common microbes associated with wound infectionin both humans (Staphylococcus aureus, Pseudomonas aeruginosa) and canines (Staphylococcus pseudintermedius, Malassezia pachydermatous). This study also assessed the ability of these botanical extracts to improve the rate of wound healing using an in vitro cell culture 'scratch' assay in both human and canine keratinocytes (HACAT and CPEK cell lines, respectively). Results demonstrated that extracts from Eucalyptus globulus, Juglans nigra, Larrea tridentata, Salvia officinalis, and Zingiber officinale had broad antimicrobial activity, while extracts from Achillea millefolium, Aloe vera, and Usnea barbata improved the rate of wound closure. The results support the development of a potential formulation of botanical extracts that may reduce infection while improving wound closure.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"55"},"PeriodicalIF":3.3,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11829538/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143424861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of add-on therapy with leukotriene receptor antagonists and Chungsangboha-tang in patients with asthma: a protocol for a randomized, placebo-controlled, parallel, multicenter trial.","authors":"Sung-Woo Kang, Hae-Seong Nam, Yang-Chun Park, Jun-Yong Choi, Ki-Tae Kim, Seo-Jung Ha, Kwan-Il Kim, Hee-Jae Jung, Beom-Joon Lee","doi":"10.1186/s12906-025-04799-w","DOIUrl":"10.1186/s12906-025-04799-w","url":null,"abstract":"<p><strong>Background: </strong>Asthma is a chronic disease characterized by airway inflammation and obstruction. Treatment aims to control symptoms with minimal medication, using disease-controlling and symptom-relieving drugs. Inhaled steroids and beta2 agonists are common treatments; however, their long-term use can cause side effects. Leukotriene receptor antagonists (LTRAs) are used in combination with inhaled steroids to manage asthma because of their anti-inflammatory and bronchodilatory effects. Combining LTRAs with Chungsangboha-tang (CSBHT), a Korean medicine, may enhance their efficacy. This study aimed to evaluate the potential of CSBHT as an adjunctive therapy for asthma management in a randomized, placebo-controlled, double-blind, multicenter clinical trial.</p><p><strong>Methods: </strong>This randomized, placebo-controlled, double-blind, parallel-group, multicenter study aims to evaluate the efficacy and safety of CSBHT as an additional treatment for patients with asthma, particularly for those with LTRAs. Overall, 198 participants will be randomly divided into intervention and control groups, with the former receiving CSBHT thrice daily and the latter receiving a placebo. Follow-ups at weeks 0, 4, and 8 will include outcome measurements, medication dispensation, and adverse reaction monitoring. The primary outcome is the mean change in forced expiratory volume in one-second scores, with secondary outcomes including changes in peak expiratory flow, forced vital capacity, forced expiratory flow 25-75%, fractional exhaled nitric oxide, Asthma Control Test, Asthma Quality of Life Questionnaire, serum IgE, eosinophil count, C-reactive protein, rescue medication usage, and a descriptive analysis of the questionnaire on asthma symptoms in Korean medicine. Safety assessments will be conducted using laboratory tests, vital signs, and monitoring of adverse events. Economic evaluations will be conducted using either cost-minimization analysis or cost-utility analysis.</p><p><strong>Discussion: </strong>This trial will evaluate the efficacy, safety, and cost-effectiveness of CSBHT as an add-on therapy to LTRAs to establish its potential as an adjuvant therapy in asthma management.</p><p><strong>Trial registration: </strong>This study was registered in the Clinical Research Information Service of Korea (KCT0006005), on March 16, 2021.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"54"},"PeriodicalIF":3.3,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11829457/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143424856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Traditional medicine regulation status and challenges in Malawi and Nigeria.","authors":"J S Mponda, A S Muula, A Choko, A J Ajuwon, J O Moody","doi":"10.1186/s12906-025-04812-2","DOIUrl":"10.1186/s12906-025-04812-2","url":null,"abstract":"<p><strong>Background: </strong>The World Health Organization encourages governments to develop legal frameworks for the regulation of traditional medicines to ensure safety, quality, and efficacy. There is inadequate published research on the traditional medicine legislative tools and Good manufacturing practices compliance by manufacturers in many countries in Sub-Saharan Africa. The study assessed the traditional medicine regulatory status and challenges in Malawi and Nigeria.</p><p><strong>Methods: </strong>A descriptive cross-sectional study was done using a structured questionnaire and a checklist of regulatory requirements to collect data from officers, manufacturing facilities, and herbal medicinal products. Twenty-three officers from the Medicines Regulatory Authorities and Ministries of Health from Malawi and Nigeria were interviewed. Thirty-one small-scale manufacturers, sixty-six herbal medicinal products from Malawi, and twenty-eight herbal medicinal products from Nigeria, that were used by people living with HIV and AIDS, were evaluated.</p><p><strong>Results: </strong>The Pharmacy and Medicines Regulatory Authority scored 7.7% in Malawi while the National Agency for Food and Drug Administration and Control in Nigeria, scored 77.0% on the 13-item regulatory checklist of regulatory requirements. The Ministry of Health, Malawi scored 28.6% while Nigeria scored 71.4% on the 7-item checklist of regulatory requirements. Both countries have no regulations on traditional medicine practice, and traditional medicine has not been integrated into the healthcare system. Most Malawian manufacturers showed poor compliance, with deficiencies observed in personnel hygiene (58.6%), cleaning production equipment (69.0%), storage rooms (72.4%), and documentation on standard operating procedures for cleaning equipment and premises (93.1%). Labelling non-compliance was observed in Malawi and Nigeria, respectively, for the list of active ingredient names (45.5% vs. 93.0%, p < 0.001), quantities of active ingredients (12.1% vs. 53.6%, p < 0.001), side effects (9.1% vs. 57.1%, p < 0.001), and storage conditions (15.2% vs. 67.9%, p < 0.001).</p><p><strong>Conclusion: </strong>Malawi lacks a strong regulatory framework for herbal medicines, with poor compliance to current Good Manufacturing Practices standards in manufacturing and labelling, posing serious public health risks. Urgent action is needed to establish comprehensive regulations, including guidelines for registering herbal medicines used by people living with HIV and AIDS. While Nigeria has a more developed regulatory framework, enforcement remains a challenge. Strengthening inspections and ensuring adherence to safety and quality standards for herbal products is critical to safeguarding public health and building consumer confidence in both countries' traditional medicine industries.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"53"},"PeriodicalIF":3.3,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11829355/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143424875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of the Qigong Tuina therapy on patients with Chronic fatigue syndrome: study protocol for a randomized controlled trial.","authors":"Haotian Han, Guanghui An, Jiahe Cui, Fangfang Xie, Hongyu Yue, Chaoqun Xie, Jianwen Ma, Guangdong Wang, Fei Yao","doi":"10.1186/s12906-025-04800-6","DOIUrl":"10.1186/s12906-025-04800-6","url":null,"abstract":"<p><strong>Background: </strong>Chronic fatigue syndrome (CFS) is a chronic disease characterized by fatigue, complex symptoms and long duration. It incurs significant economic costs every year. Qigong Tuina therapy (QTT), a traditional Chinese therapy, is skilled in the treatment of fatigue. The objective of this study is to observe the effectiveness of QTT in treating patients with CFS.</p><p><strong>Methods: </strong>A randomized controlled trial will recruit 128 patients with CFS. The patients will be allocated randomly in a 1:1 ratio to either the QTT group or the cognitive behavioral therapy (CBT) group. The interventions for both groups will be carried out once per week for 8 weeks. Then a 4 weeks follow-up will be conducted for all patients after the intervention. The primary outcome will be the changes in the Multidimensional Fatigue Inventory (MFI-20). Secondary outcomes will be measured by the 36-Item Short Form Health Survey (SF-36), Pittsburgh Sleep Quality Index (PSQI), Hospital Anxiety and Depression Scale (HADS), and functional magnetic resonance imaging (fMRI). The data will be analyzed at the baseline, 4 weeks into the intervention, at the end of the intervention, and 4 weeks after the intervention. The safety of interventions will be assessed after each treatment session.</p><p><strong>Discussion: </strong>The trial aims to establish whether QTT is not inferior to CBT for CFS patients. This study provides vital information for an alternative and complementary therapy aimed at patients afflicted with CFS.</p><p><strong>Ethics and dissemination: </strong>Ethical approval was granted by Ethics Committee of Shanghai Municipal Hospital of Traditional Chinese Medicine (2023SHL-KY-100-02). The findings will be disseminated through publications, conferences, and briefs to professional organizations.</p><p><strong>Trial registration: </strong>ChiCTR2400081828, Chinese Clinical Trial Registry. Registered on March 13, 2024.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"57"},"PeriodicalIF":3.3,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11829471/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143424859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid shift of gut microbiome and enrichment of beneficial microbes during arhatic yoga meditation retreat in a single-arm pilot study.","authors":"Sanjay Swarup, Abhishek Gupta, Marianne Chung, Vaishnavi Radhakrishnan, Valerie Davis, Michael D J Lynch, Trevor C Charles, Jiujun Cheng, Glenn Mendoza","doi":"10.1186/s12906-025-04783-4","DOIUrl":"10.1186/s12906-025-04783-4","url":null,"abstract":"<p><strong>Background: </strong>The human microbiome plays a vital role in human health, mediated by the gut-brain axis, with a large diversity of functions and physiological benefits. The dynamics and mechanisms of meditations on oral and gut microbiome modulations are not well understood. This study investigates the short-term modulations of the gut and oral microbiome during an Arhatic Yoga meditation retreat as well as on the role of microbiome in improving well-being through a possible gut-brain axis.</p><p><strong>Methods: </strong>A single-arm pilot clinical trial was conducted in a controlled environment during a 9-day intensive retreat of Arhatic Yoga meditation practices with vegetarian diet. Oral and fecal samples of 24 practitioners were collected at the start (Day0: T1), middle (Day3: T2), and end (Day9:T3) of the retreat. Targeted 16S rRNA gene amplicon sequencing was performed for both oral and gut samples. Functional pathway predictions was identified using phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt2). DESeq2 was used to identify the differential abundant taxa. Various statistical analyses were performed to assess the significant changes in the data.</p><p><strong>Results: </strong>Our findings revealed that Arhatic Yoga meditation together with a vegetarian diet led to changes in the oral and gut microbiome profiles within the 9-day retreat. Oral microbiome profile showed a significant (p < 0.05) difference in the species richness and evenness at the end of study, while non-metric multidimensional scaling (NMDS) confirmed the shift in the gut microbiome profile of the practitioners by T2 timepoint, which was further supported by PERMANOVA analysis (p < 0.05). Health-benefiting microbes known to improve the gastrointestinal and gut-barrier functions, immune modulation, and gut-brain axis were enriched. Gut microbiome of both beginner and advanced Arhatic Yoga practitioners showed similar trends of convergence by the end of study. This implies a strong selection pressure by Arhatic Yoga meditation together with a vegetarian diet on the beneficial gut microbiome.</p><p><strong>Conclusion: </strong>This pilot study demonstrates that Arhatic Yoga meditation practices combined with a vegetarian diet during a short intensive retreat resulted in enrichment of known health-promoting microbes. Such microbial consortia may be developed for potential health benefits and used as probiotics to improve the gastrointestinal and immune systems, as well as functions mediated by the gut-brain axis.</p><p><strong>Trial registration: </strong>Study was submitted in https://clinicaltrials.gov/on28-02-2024 . Retrospective registered.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"51"},"PeriodicalIF":3.3,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11823196/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143405615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring and determining traditional medicines used for bleeding control and termination of pregnancy among Suri people, South West Ethiopia, 2022: mixed method exploratory sequential study design.","authors":"Alemnew Wale, Aychew Kassie, Nahom Solomon, Desalegn Girma","doi":"10.1186/s12906-025-04795-0","DOIUrl":"10.1186/s12906-025-04795-0","url":null,"abstract":"<p><strong>Background: </strong>The treatment of severe bleeding, stopping pregnancies, and other gynecologic conditions have all been addressed by women using traditional medicines and methods. Even though research has been done to explore these concerns more, evidence is limited in developing countries, particularly in Ethiopia Hence, this study sought to investigate the traditional treatments and remedies employed by women in pastoralist communities living in southwestern Ethiopia.</p><p><strong>Objective: </strong>To explore and determine traditional medicines used for bleeding control and termination of pregnancy among Suri People, South West Ethiopia, 2022.</p><p><strong>Method: </strong>A mixed-method exploratory sequential study design was conducted at selected kebeles of Suri District, South West, Ethiopia, from February 1 to August 30, 2022. Convenience and purposive sampling techniques were implemented for quantitative and qualitative studies, respectively. SPSS version 26 and Atlas.ti version 22 software were used for data analysis for the quantitative and qualitative parts, respectively.</p><p><strong>Result: </strong>60 People participated in the qualitative study and 350 women participated in the quantitative study, making a 100% response rate. Qualitatively Blood tree, Shawly tree, and Sitch lady were utilized for bleeding control, and, the root of banana, the root of maize, Quartum, Teteracycle with alcohol, boiled alcohol, soot grass leave olive and salty soil were used for pregnancy termination. Quantitatively the proportion of women who used traditional medicines for abortion, and bleeding control were 38%, and 36% respectively. Blood tree (28%),Shawly tree(38%), and Sitch lady(34%) were the most commonly reported traditional medicines or methods used for bleeding control. Similarly, the root of banana (15%), the root of maize (19%), Teteracycle with alcohol (14%), Quartum (17%), boiled alcohol (15%), and soot grass (20%).</p><p><strong>Conclusion: </strong>Suri women use various plants, alcohol, and drugs to interrupt pregnancy as well as blood and shawily trees to manage bleeding during (heavy menstrual, abortion, and postpartum periods). To help people use safe methods and avoid those that have a negative impact on mothers, it is imperative to investigate more about the value of these traditional methods. Further botanical study is recommended to scientifically evaluate the traditional medicine obtained from different plants.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"52"},"PeriodicalIF":3.3,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11827227/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143413024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pulsed electromagnetic fields modulate energy metabolism during wound healing process: an in vitro model study.","authors":"Feng Liao, Yamei Li, Zhou Zhang, Qian Yu, Huifang Liu","doi":"10.1186/s12906-025-04792-3","DOIUrl":"10.1186/s12906-025-04792-3","url":null,"abstract":"<p><strong>Background: </strong>Pulsed electromagnetic fields (PEMFs) therapy was extensively investigated to treat wound healing, which is a highly metabolically demanding process. However, the effect of PEMFs on energy metabolism in wound healing remains largely unexplored. Therefore, our study aims to demonstrate the role of PEMFs on energy metabolism in wound healing.</p><p><strong>Methods: </strong>Scratch-wound healing assay and cell viability assay were performed for the in vitro study of the effect of PEMFs on cell migration and viability. Seahorse assay was conducted for energy metabolism analysis while holo-tomographic microscopy for fine changes of L929 cells. Mitochondrial membrane potential assay and intracellular reactive oxygen species (ROS) and pH assay were performed for analyzing the changes of mitochondrial function.</p><p><strong>Results: </strong>PEMFs with specific parameter (4mT, 80 Hz) promoted cell migration and viability. Glycolysis stress and mitochondria stress test revealed that PEMFs-exposed L929 cells was highly glycolytic for energy generation. Besides, PEMFs enhanced intracellular acidification and maintained low level of intracellular ROS in L929 cells. Compared to control group, much more vesicles were generated and then transported to regions close to the nuclear in L929 cells treated with PEMFs.</p><p><strong>Conclusions: </strong>Our major findings revealed for the first time that PEMFs induce metabolic reprogramming of fibroblast shifting from mitochondrial respiration to glycolysis, accompanied with an increase of vesicular transport, which is closely related to wound healing in vitro.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"50"},"PeriodicalIF":3.3,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11823022/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143405614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ribes nigrum leaf extract downregulates pro-inflammatory gene expression and regulates redox balance in microglial cells.","authors":"Alvard Minasyan, Vivien Pires, Catherine Gondcaille, Mikayel Ginovyan, Marika Mróz, Stéphane Savary, Mustapha Cherkaoui-Malki, Barbara Kusznierewicz, Agnieszka Bartoszek, Pierre Andreoletti, Naira Sahakyan","doi":"10.1186/s12906-025-04780-7","DOIUrl":"10.1186/s12906-025-04780-7","url":null,"abstract":"<p><strong>Background: </strong>This study focuses on the investigation of the antioxidant and anti-inflammatory activities of alcohol extracts from Ribes nigrum leaves on murine BV-2 microglial Wt and Acyl-CoA oxidase 1 deficient (Acox1^-/-) cell line models, useful for the investigation of some neurodegenerative disorders.</p><p><strong>Methods: </strong>The extract chemical composition was analyzed via LC-Q-Orbitrap HRMS. Various assays, including DPPH, MTT, and H₂DCFDA, were used to assess the extract's antioxidant capacity, cell viability, and reactive oxygen species (ROS) production. Immunoblotting and RT-qPCR techniques were employed to measure protein expression and gene transcription in treated cells. Statistical analysis was conducted using GraphPad Prism, with significance determined at p < 0.05.</p><p><strong>Results: </strong>Investigations showed the presence of phenolic compounds in this extract, among which flavan-3-ols, flavonols, furanocoumarins, hydroxycinnamates were major components, which are known for their biological activity in various test systems. The MTT test revealed a concentration of 0.125 mg/mL of R. nigrum extract as the highest non-toxic. The investigated extract showed high antioxidant activity in chemical-based tests. The antioxidant potential of the R. nigrum leaf extract was furtherly explored using the BV-2 microglial cell line models. Moreover, the extract was found to alter the activity of the main antioxidant enzyme, catalase and fatty acid oxidation enzyme, Acyl-CoA oxidase 1 (ACOX1) as well as the expression of appropriate genes in Wt and Acox1^-/- BV-2 microglial cells such as Cat, iNos, Il-1β, Tnf-α, and Abcd1. In Wt cells, after the 24-hour treatment with R. nigrum leaf extract, ACOX1 activity was downregulated, meanwhile the catalase activity remains unchanged. Further treatment led to the downregulation of catalase and the upregulation of ACOX1 activity. However, in Acox1^-/- cells, which represent a model of oxidative stress, an increase in catalase activity was observed only after 48 h of treatment. It was also observed the reduced ROS and NO formation in cells, showing the pronounced antioxidant capacity of R. nigrum extract in the investigated cell-models.</p><p><strong>Conclusion: </strong>Our study demonstrated the protective effects of R. nigrum leaf extracts on BV-2 microglial cells by reducing oxidative and nitrosative stress, decreasing pro-inflammatory gene expression, and normalizing peroxisomal function, highlighting the potential of these extracts as therapeutic agents for managing oxidative stress and inflammation.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"49"},"PeriodicalIF":3.3,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11823126/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143405616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}