BMC Complementary Medicine and Therapies最新文献

{"title":"Bioprospection of indigenous herbal formulations for diabetes care: in vitro, network pharmacology, and molecular dynamics studies.","authors":"Oluwafemi Adeleke Ojo, Akingbolabo Daniel Ogunlakin, Omolola Adenike Ajayi-Odoko, Gideon Ampoma Gyebi, Damilare IyinKristi Ayokunle, Adesoji Alani Olanrewaju, Oluwatobi Deborah Agbeye, Emmanuel Tope Ogunwale, Oluyomi Stephen Adeyemi","doi":"10.1186/s12906-025-04980-1","DOIUrl":"10.1186/s12906-025-04980-1","url":null,"abstract":"","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"225"},"PeriodicalIF":3.3,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12224487/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144559145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A systematic review of flotation-restricted environmental stimulation therapy (REST).","authors":"Elnaz Lashgari, Emma Chen, Jackson Gregory, Uri Maoz","doi":"10.1186/s12906-025-04973-0","DOIUrl":"10.1186/s12906-025-04973-0","url":null,"abstract":"<p><strong>Background: </strong>Restricted Environmental Stimulation Therapy (REST) is a therapeutic technique that involves immersing an individual in an environment with minimal sensory input or stimulation. The goal of REST is to induce a state of relaxation that is deeper than what can be achieved through other forms of relaxation techniques. Research suggests that REST can help reduce anxiety, alleviate chronic pain, improve sleep, and enhance creativity and cognitive function. Flotation-REST is a popular type of REST that utilizes an enclosed tank filled with buoyant saltwater to facilitate relaxation. This study aimed to synthesize the evidence on studies that investigate the effects of flotation-REST.</p><p><strong>Methods: </strong>We used Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) to survey the flotation-REST literature starting from the earliest papers we could find, in 1960, until May 2024. This search was conducted on 23 May 2024 within the Google Scholar and PubMed databases. Journal and conference papers, as well as electronic preprints, that used flotation-REST in their methods, and that were written in English were included; non-original research papers (e.g., review papers, book chapters, and papers solely on types of REST other than flotation-REST (e.g., chamber-REST) were excluded. From each eligible paper, we extracted information regarding the participant sample, application of flotation-REST, experimental design, treatment delivery method, questionnaires and tools, and study results.</p><p><strong>Results: </strong>In total, we found 63 studies that included 1,838 participants. We propose that the application of flotation-REST can be divided into nine main categories: pain, athletic performance, physiology, stress, consciousness, psychology, creativity, clinical anxiety, sleep, smoking cessation, and other miscellaneous applications. In general, flotation-REST was found to bring about positive effects on pain, athletic performance, stress, mental well-being, and clinical anxiety, while having limited to no effect on sleep-related disorders and smoking cessation.</p><p><strong>Conclusion: </strong>This paper provides a comprehensive overview of the current research on flotation-REST, highlights ongoing limitations in the literature, and outlines potential areas for future research. While flotation-REST appears to induce various benefits for physical and mental well-being, particularly when it comes to managing states like pain and stress, more research is needed to better understand the mechanisms underlying these effects and to identify optimal treatment protocols for different populations. A limitation of this paper is the relatively small number of studies available for review, which limits the generalizability of certain findings and highlights the need for additional research in this area.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"230"},"PeriodicalIF":3.3,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12224670/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144559144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Can heart rate variability demonstrate the effects and the levels of mindfulness? A repeated-measures study on experienced and novice mindfulness practitioners.","authors":"Yanping Wei, Yifei Xu, Wanlin Chen, Jing Zheng, Hang Chen, Shulin Chen","doi":"10.1186/s12906-025-04972-1","DOIUrl":"10.1186/s12906-025-04972-1","url":null,"abstract":"<p><strong>Background: </strong>Heart rate variability (HRV) is a potential biomarker that might demonstrate the effects of mindfulness, but it might be influenced by practice experiences. This study wanted to elucidate the possibility of using HRV metrics to reveal the effects of mindfulness and examine its variation between novice and experienced mindfulness practitioners.</p><p><strong>Methods: </strong>Forty-six participants (20 experienced practitioners, 26 novices) were enrolled to practice 14-day mindfulness training. HRV data were collected during three phases (20 min baseline, T1; 20 min mindfulness, T2; 20 min post-mindfulness, T3) using Holter monitoring. The linear mixed model was conducted to explore the effects of group and time based on standardized data.</p><p><strong>Results: </strong>The experienced group had higher full-scale scores of FFMQ both in the pre-test (t = -3.34, df = 44, p = 0.002) and the post-test (t = -2.35, df = 44, p = 0.025). Both groups showed significant changes in HRV indices (e.g., RMSSD, SDNN, LnHF) from T1 to T2 or T3 (p < 0.05). In the experienced group, significant fluctuations (p < 0.05) were observed at T2, followed by recovery at T3, in SD1/SD2, Sample Entropy, normalized High Frequency (HFn), DFA_α1, and DFA_α2. In contrast, the novice participants only showed monotonic changes in SD1/SD2 and DFA_α1.</p><p><strong>Conclusions: </strong>This study revealed significant HRV changes during mindfulness practice, with distinct patterns observed between novice and experienced practitioners.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"231"},"PeriodicalIF":3.3,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12225205/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144559146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of auriculotherapy on urinary incontinence in men with prostate cancer: a randomized controlled trial.","authors":"Ying Zhang, Siyuan Wu, Keping Zhu, Kun Li, Ziyi Qi, Binbin Zhu, Sijia Hou, Wei Wang","doi":"10.1186/s12906-025-04979-8","DOIUrl":"10.1186/s12906-025-04979-8","url":null,"abstract":"<p><strong>Background: </strong>Urinary incontinence is one of the most distressing common symptoms in patients with prostate cancer after surgery. Auriculotherapy has been shown to improve a variety of symptoms effectively and has a certain potential in managing urinary incontinence, but the relevant studies are few and inconclusive. This study aimed to assess the effectiveness of auriculotherapy on urinary incontinence in men with prostate cancer.</p><p><strong>Methods: </strong>Participants were recruited from the urology ward of a tertiary hospital between October 2023 and April 2024. A total of 114 men who underwent radical prostatectomy were randomly assigned to the usual care (n = 38), sham-auriculotherapy (n = 38), or true-auriculotherapy (n = 38) groups. All groups received pelvic floor muscle training, and the intervention groups received four additional sham-auriculotherapy or true-auriculotherapy. The primary outcomes were the International Advisory Committee on Urinary Incontinence Questionnaire short form (ICIQSF) score and clinical effective rate, the secondary outcomes included the continence rate, pad usage, urinary incontinence severity, urinary incontinence episodes, and quality of life. Data were collected at week 2, week 4, week 8, and week 12 after surgery. Generalized estimating equations were applied for outcome comparison.</p><p><strong>Results: </strong>The true-auriculotherapy group had significantly lower ICIQSF scores (β = -1.93, 95% CI: -3.21, -0.65, P = 0.030), higher clinical effective rates (OR = 1.12, 95% CI: 1.04, 1.20, P = 0.003), lower pad uses (β = -0.50, 95% CI: -0.93, -0.07, P = 0.023), and higher Incontinence Quality of Life Instrument scores (β = 3.82, 95% CI: 1.55, 6.09, P = 0.001) than usual care group, while no significant difference was noted between the sham-auriculotherapy and usual care groups. The continence rates were not significantly different among the three groups. In terms of the simplified version of the Expanded Prostate Cancer Review Index, the true-auriculotherapy exhibited greater improvements in the \"urinary incontinence\", \"urinary irritation /obstruction\", and \"hormonal\" domains than usual care at week 12, but showed no significant differences regarding \"bowel\" and \"sexual\" domains. The sham-auriculotherapy was superior to usual care only in the \"hormonal\" domain and was not significantly different in the other domains.</p><p><strong>Conclusion: </strong>Auriculotherapy is a relatively simple, low-cost, low-risk, and effective nonpharmacological approach for alleviating urinary incontinence. Regular and consistent acupressure is essential to maintain the effectiveness of auriculotherapy. The long-term effectiveness and mechanism of auriculotherapy need to be further explored.</p><p><strong>Trial registration: </strong>ChiCTR2300071700, available on the Chinese Clinical Trial Registry ( www.chictr.org.cn ). The first registration date was 23/05/2023.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"234"},"PeriodicalIF":3.3,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12231745/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144559074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative study on the therapeutic potential of aqueous extracts from commercially cultivated Agaricus bisporus and Lentinula edodes in Sri Lanka: antioxidant, anticancer, antidiabetic, and antibacterial properties.","authors":"G V S Pabasara, M D M Fernando, W K S M Abeysekera, L V C Liyanapathirana","doi":"10.1186/s12906-025-04984-x","DOIUrl":"10.1186/s12906-025-04984-x","url":null,"abstract":"","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"233"},"PeriodicalIF":3.3,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12231662/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144559147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treatment of rheumatoid arthritis by Rhododendron molle G. Don: the anti-inflammatory mechanism of its extract was elucidated by network analysis combined with experimental verification.","authors":"Wenya Mei, Junbao Yu, Jiayuan Zhu, Ting Deng, Yasi Deng, Qiaozhen Tong, Xiangdan Liu, Huanghe Yu, Ribao Zhou, Xiaorong Liu","doi":"10.1186/s12906-025-04982-z","DOIUrl":"10.1186/s12906-025-04982-z","url":null,"abstract":"<p><strong>Objective: </strong>We investigated the underlying mechanism by which Rhododendron molle G. Don extraction (RME) mitigates rheumatoid arthritis (RA) in rats through network analysis and molecular docking.</p><p><strong>Methods: </strong>The active metabolites of RME were analyzed by high performance liquid chromatography - evaporative light scattering detector (HPLC-ELSD). Subsequently, the potential targets were screened from various databases. Genes relative to RA and GSE55457 data sets were obtained. Furthermore, the biological processes associated with the drug targets and GSE55457 genes were analyzed. A protein - protein interaction (PPI) network was constructed and subjected to topological analysis. Key proteins within this network were identified and validated by in vivo experiments.</p><p><strong>Results: </strong>HPLC analysis showed that REM contained multiple anti-inflammatory active metabolites. Through network analysis, we found that the potential target of the drug and the differential genes of RA were involved in several signaling pathways, including Wnt, JAK-STAT and other signaling pathways which are known to play important roles in inflammatory responses. Dvl1 and GSK-3β were screened as the core targets by topological analysis of the potential target protein interaction network. The results of molecular docking showed that the core target had good affinity with the active metabolites of R. molle. It was confirmed that RME could remarkably downregulate the expression of Dvl1, Wnt1, p-GSK-3β, leading to a marked reduction in toe thickness and arthritis index (AI) of AIA rats, reducing the level of inflammatory factors, and alleviating the degree of tail ulceration in AIA rats in a dose-dependent manner, displaying therapeutic effects comparable to those of Tripterygium wilfordii polyglycoside tablets.</p><p><strong>Conclusion: </strong>RME contains active metabolites with anti-inflammatory effects, which can significantly inhibit inflammation through multiple targets and pathways to effectively ameliorate RA.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"235"},"PeriodicalIF":3.3,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12232070/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144559080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Public self-reported adverse experience and knowledge about use of herbal and dietary supplements.","authors":"Kamonphat Wongtaweepkij, Satchawit Srinonghang, Wiriya Youngpattana, Krittin Summa, Sonthiya Papenkort, Anthony R Cox, Narumol Jarernsiripornkul","doi":"10.1186/s12906-025-04971-2","DOIUrl":"10.1186/s12906-025-04971-2","url":null,"abstract":"<p><strong>Background: </strong>Herbs and dietary supplement (HDS) use has become increasingly used among the Thai population due to greater health awareness and easier product access. However, research on adverse events from HDS and information needs relating to HDS among the Thai population is limited. This study aims to explore use, adverse experiences, self-assessed knowledge and perspectives on HDS among the general public in Thailand.</p><p><strong>Methods: </strong>A cross-sectional self-administered survey in the general public conducted in 6 public areas in a large city of northeastern Thailand, over 5-month period. It explored experiences relating to use and adverse effects of HDS. Knowledge and information needs relating to HDS were self-assessed by using a visual analog scale and closed questions. The perspectives on HDS were determined using the 5 point-Likert scales for degree of agreement. Participants were selected by purposive sampling.</p><p><strong>Results: </strong>Of the 1,064 questionnaires distributed, 540 (51.0%) respondents reported using herbal and dietary supplements (HDS). Among them, 363 (67.2%) used herbs, and 423 (78.3%) used dietary supplements, with 40.3% using these products infrequently in the past six months. Common herbs included andrographis (25.3%), turmeric (21.2%), and senna (6.9%), while vitamin C (41.8%), collagen (11.8%), and vitamin B complex (9.0%) were the most frequently used dietary supplements. Adverse effects were reported by 42 respondents, primarily associated with turmeric (25.0%) and vitamin C (31.8%), with most rating the severity as mild (76.2%). Self-assessed knowledge about HDS use and adverse effects was moderate, with a significant demand for information on interactions with medications (59.3%), adverse effects (57.0%), and actions to take if adverse effects occur (52.8%). Higher education levels correlated with increased in knowledge about HDS use and adverse effects (p = 0.007 and p = 0.001, respectively).</p><p><strong>Conclusions: </strong>Approximately half of the general public in Thailand use HDS, with a small number of reporting adverse events, primarily mild gastrointestinal effects. The public has a moderate level of knowledge about the use and adverse effects of HDS, but there is a need for improved safety knowledge relating to HDS.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"226"},"PeriodicalIF":3.3,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12224633/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144559077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Traditional bone setting in Nigeria from the perspectives of patients and physiotherapists- clinical insights for low back pain management.","authors":"Mishael Adje, Sven Karstens, Chidozie Mbada, Jost Steinhäuser","doi":"10.1186/s12906-025-04966-z","DOIUrl":"10.1186/s12906-025-04966-z","url":null,"abstract":"<p><strong>Background: </strong>Traditional bone setting (TBS) remains a prevalent healthcare practice in Nigeria, offering complementary treatments for musculoskeletal conditions such as low back pain (LBP). This study explores the perspectives of both patients and physiotherapists regarding TBS and its implications for the management of LBP.</p><p><strong>Methods: </strong>A qualitative research approach was employed, utilizing semi-structured interviews with 25 participants (13 patients who had utilized TBS services for LBP, and 12 physiotherapists). Theoretical sampling was employed in participant recruitment until saturation. Recordings were transcribed and thematic analysis was conducted as a secondary analysis. Reporting was informed by the Consolidated Criteria for Reporting Qualitative Research (COREQ).</p><p><strong>Results: </strong>Eleven (11) participants were female and 14 were male; with a mean age of 35 years. Five themes were identified from this study: driving impetus for TBS, influencing perceptions with information, turning to TBS as a final recourse, exploring the primary alternative, and integrating TBS. The shift towards TBS for LBP stems from deficiencies in prevailing healthcare practices in Nigeria. Patients and physiotherapists hold varying perspectives regarding cultural significance, and effectiveness and safety of TBS compared to professional physiotherapy interventions. Accessibility, affordability, and perceived efficacy are common facilitators for the patronage of TBS among patients, while others were hesitant and viewed it as a last resort. Negative perception of physiotherapists about TBS for LBP were based on concerns regarding the lack of scientific evidence, standardized practices, and potential complications associated with the procedures.</p><p><strong>Conclusion: </strong>Participants consider TBS viable when professional physiotherapy fails, citing expectations for therapist-guided techniques, affordability, and cultural factors. Physiotherapists remain cautious, stressing the need for evidence-based care and noting severe TBS complications. Some participants advocate for integrating TBS with professional healthcare through collaboration and better communication. This study, supported by literature, highlights the potential for TBS integration, with open communication and training fostering collaboration. Future studies could investigate the practicality of this integration, prioritizing culturally appropriate, safe, and effective approaches to LBP management.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"229"},"PeriodicalIF":3.3,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12224792/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144559079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of acupressure on pain, menstrual symptoms, and comfort in adolescents with primary dysmenorrhea: a single-blind randomized controlled trial.","authors":"Ahu Aksoy-Can, Aysu Buldum, Arzu Abiç, Duygu Vefikuluçay-Yilmaz","doi":"10.1186/s12906-025-04965-0","DOIUrl":"10.1186/s12906-025-04965-0","url":null,"abstract":"<p><strong>Background: </strong>Integrative therapies, such as acupressure, may be employed for the management of primary dysmenorrhea (PD), which is a prevalent condition with a particularly high prevalence among adolescents.</p><p><strong>Aim: </strong>This study was conducted as a single-blind randomized controlled trial to determine the effect of acupressure on pain, menstrual symptoms, and comfort in adolescents with PD.</p><p><strong>Design: </strong>A two-arm randomized controlled trial.</p><p><strong>Methods: </strong>A total of 56 female adolescent students were randomly assigned to either the acupressure group (n = 28) or the sham group (n = 28) using block randomization. The acupressure group applied self-acupressure to the LI4, ST36, and SP6 points, while the sham group applied self-sham acupressure to points 1.5 cm around the same points for a period of three menstrual cycles (12 weeks) for an average of 15 min, immediately after waking up and just before going to bed on the first three days of menstruation. The primary outcome was Visual Analog Scale (VAS), and the secondary outcome were Menstruation Symptom Questionnaire (MSQ) and the General Comfort Questionnaire (GCQ).</p><p><strong>Results: </strong>A comparison of the acupressure group with the sham group revealed a statistically significant decline in VAS and MSQ scores, while GCQ scores demonstrated a notable increase at the end of the 12-week study period (p < 0.05). An intragroup comparison revealed a statistically significant decrease in the VAS scores of the acupressure group over time (p < 0.001). For both groups, there was a decrease in the MSQ scores and an increase in the scores obtained from the socio-cultural domain subscale of the GCQ over time (p < 0.05).</p><p><strong>Conclusion: </strong>The results demonstrated that acupressure is an efficacious approach for alleviating pain and menstrual symptoms, as well as enhancing comfort. Consequently, regular acupressure may be a viable option for the management of PD in young women.</p><p><strong>Trial registration: </strong>This randomized controlled trial was conducted in accordance with the Consolidated Standards of Reporting Trials (CONSORT) guidelines and registered with ClinicalTrials.gov (NCT05408611) on dated 01.06.2022.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"221"},"PeriodicalIF":3.3,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12224516/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144552199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antiproliferative activities of phytochemicals isolated from the leaves of Dolichos kilimandischaricus (Harms) ex Taub. (Fabaceae) on Jurkat and HL-60 leukemic cells.","authors":"Simbarashe Sithole, Paul Mushonga, Matthias Heydenreich, Cephas Mawere, Stanley Mukanganyama","doi":"10.1186/s12906-025-04977-w","DOIUrl":"10.1186/s12906-025-04977-w","url":null,"abstract":"<p><strong>Background: </strong>Natural plant products represent one of the most productive sources of innovative lead drugs for the treatment of a wide range of ailments. Dolichos kilimandischaricus (Harms) ex Taub. (Fabaceae) root extracts have been traditionally used for the treatment of HPV-related cancers. The extracts from the plant have been previously shown to have antiproliferative effects on cancer cell lines. This study aimed to isolate, purify, and analyze the phytochemicals from the leaves of D. kilimandischaricus.</p><p><strong>Methods: </strong>Phytochemicals in the ethanol leaf extract were separated by column chromatography on silica gel. The structures of the compounds were elucidated using spectroscopic techniques, then compared with the reported spectral data. Cytotoxicity of the phytochemicals on HL-60 and Jurkat cells was assessed by the sulphorhodamine B (SRB) assay with chlorambucil as a positive control. Docking simulations were used to further understand the binding preferences of the isolated compounds.</p><p><strong>Results: </strong>The isolated compounds were identified as 3β-stigmasterol (1) and α-spinasterol (2). The results showed that both compounds were more potent against HL-60 cells than Jurkat cells, with IC₅₀ values of 9.49 and 8.66 µg/mL, respectively. The two compounds function as 1HJC and 6VG8 inhibitors, according to the docking results, with the highest negative molecular binding affinities shown for both ligands against 1HJC.</p><p><strong>Conclusions: </strong>Therefore, this study has identified some of the phytochemicals that may be responsible for the antiproliferative activity in D. kilimandischaricus. These phytochemicals may provide leads in the development of compounds for treating cancer and related neoplastic diseases.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"224"},"PeriodicalIF":3.3,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12225472/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144552221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}