BMC Complementary Medicine and Therapies最新文献

{"title":"Compound kushen injection improves radiation enteritis via cannabinoid receptor 1 in rats.","authors":"Wenjing Xu, Liping Gao, Wenjuan Zou, Xiaohui Tang, Weiqi Nian, Weiqin Zheng, Rongzhong Huang, Pei Wang","doi":"10.1186/s12906-025-04820-2","DOIUrl":"10.1186/s12906-025-04820-2","url":null,"abstract":"<p><strong>Background: </strong>Clinical studies have shown that Compound Kushen Injection (CKI) can alleviate the inflammatory symptoms of radiation enteritis. However, the mechanism of action remains unclear. The aim of this study was to explore the possible targets and mechanisms of CKI in the treatment of radiation enteritis.</p><p><strong>Methods: </strong>Network pharmacology was used to predict the potential targets of CKI for the treatment of radiation enteritis, and GO and KEGG enrichment analyses were subsequently performed. SD rats were randomly divided into one of the following groups: control, model, CB1 agonist, CKI and CKI + CB1 antagonist. Except for the control group, the remaining groups were irradiated the abdomen with 6 MV medical high-energy x-ray linear accelerator to establish the model of radiation enteritis. After one week of treatment, the expression of inflammatory factors, SOD and GSH-Px activities, MDA, ROS and NO contents; NF-κB signaling activation and the expression of NOX4, CB1, p38 MAPK, p-p38 MAPK in the ileal tissues of rats were examined to assess the therapeutic effect and possible mechanism of CKI on radiation enteritis, respectively.</p><p><strong>Results: </strong>According to network pharmacology, CB1 might be a target of CKI. GO and KEGG enrichment analyses revealed that CKI was significantly enriched in analgesic, endocannabinoid and inflammatory pathways. In the rat model, Compared with that in the radiotherapy group, the extent of ileal injury was significantly improved in the CKI group compared to the control group. In addition, the infiltration of CD68 and CD16b was significantly reduced, and the expression of MCP1, TNF-α, IL-1β and IL-10 was significantly decreased. In addition, the activities of SOD and GSH-Px were increased, and the activities of MDA, ROS and NO were decreased. The CKI group also showed inhibition of NF-κB signaling and a significant decrease in the expression of NOX4, CB1 and p-p38 MAPK/p38 MAPK. The use of a CB1 agonist could also alleviate radiation enteritis, whereas the addition of a CB1 antagonist could interfere with the ameliorative effect of CKI on radiation enteritis.</p><p><strong>Conclusions: </strong>CKI might exert an anti-radiation enteritis effect by targeting the cannabinoid receptor 1.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"70"},"PeriodicalIF":3.3,"publicationDate":"2025-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11847357/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143476256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro analysis of the molecular mechanisms of ursolic acid against ovarian cancer.","authors":"Ru Zhang, Zhaopeng Zhang, Lulu Xie, Ziqing Yu, Rui Gao, Zhi-Run Zhang, Ying Zhang, Xuyang Wei, Yang Chen, Sue Jiao, Yiren Gao, Jun-Peng Guo","doi":"10.1186/s12906-025-04808-y","DOIUrl":"10.1186/s12906-025-04808-y","url":null,"abstract":"<p><p>Ovarian cancer is one of most common gynaecologic malignancy and ranks third in cancer-related deaths among women. Ursolic acid (UA) is a pharmacologically active pentacyclic triterpenoid isolated from a large variety of vegetables, fruits and many traditional medicinal plants. However, the mechanism of action of UA in inhibiting the proliferation of ovarian cancer cells remains unclear. Consequently, this experiment was designed to elucidate the mechanism of action of UA in inhibiting the proliferation of ovarian cancer cells in greater detail.The results indicated that UA was capable of effectively inhibiting the proliferation, migration, and colony formation of ovarian cancer cells.UA was observed to up-regulate Bcl-2-associated X protein(BAX)and cysteinyl aspartate specific proteinase 3 (Caspase3) expression and down-regulating B-cell lymphoma-2(Bcl-2) expression.Meanwhile, UA up-regulated Sequestosome 1(p62)expression and down-regulated coiled-coil, moesin-like BCL2-interacting protein(Becline1), microtubule-associated proteins light chain 3(LC3), Phosphoinositide 3-Kinase(PI3K), andProtein Kinase B( AKT) expression, thus effectively inhibiting autophagy in ovarian cancer cells.Furthermore, UA upregulated pancreatic ER kinase (PKR)-like ER kinase (PERK), eukaryotic translation initiation factor 2 A(eIF2α), and The C/EBP Homologous Protein(CHOP) expression.In addition UA upregulates PERK, eIF2α, and CHOP expression and effectively promotes endoplasmic reticulum stress(ERS).In conclusion, UA can inhibit ovarian cancer cell proliferation, migration, colony formation, and may inhibit tumor cell autophagy by promoting tumor cell ERS, and ultimately promote ovarian cancer cell apoptosis.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"65"},"PeriodicalIF":3.3,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11846399/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143472260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the therapeutic potential of marjoram (Origanum majorana L.) in polycystic ovary syndrome: insights from serum metabolomics, network pharmacology and experimental validation.","authors":"Aliaa M Elfiky, Reham S Ibrahim, Amira R Khattab, Mai O Kadry, Naglaa M Ammar, Eman Shawky","doi":"10.1186/s12906-025-04774-5","DOIUrl":"10.1186/s12906-025-04774-5","url":null,"abstract":"<p><strong>Background: </strong>Polycystic Ovary Syndrome (PCOS) is a common endocrine disorder with significant metabolic and hormonal dysregulation. Marjoram (Origanum majorana L.), known for its medicinal properties, has potential in managing PCOS through various bioactive compounds.</p><p><strong>Objective: </strong>This study aims to evaluate the effects of marjoram on PCOS symptoms using serum pharmacochemistry, network pharmacology, and molecular docking in a DHEA-induced rat model.</p><p><strong>Methods: </strong>Polycystic Ovary Syndrome (PCOS) was induced in rats using dehydroepiandrosterone (DHEA). Marjoram's therapeutic effects were evaluated by analyzing oxidative stress biomarkers, hormone levels, and ovarian histopathology. Untargeted serum metabolomics, conducted with ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC TQD-MS/MS), identified key bioactive compounds. These compounds were then examined through network pharmacology to map their interactions with PCOS-related pathways, with findings validated via molecular docking.</p><p><strong>Results: </strong>Marjoram treatment significantly reduced oxidative stress by decreasing nitric oxide (NO) and increasing total antioxidant capacity (TAC). Hormonal analysis revealed that high-dose marjoram (100 mg/kg) normalized progesterone, estradiol, testosterone and FSH levels. Body weight gain was also reduced with marjoram treatment, especially at the higher dose. Histopathological evaluation showed fewer ovarian cysts and improved follicular structure with marjoram administration. Network pharmacology analysis highlighted the steroid hormone biosynthesis and estrogen signaling pathways as critical targets, with apigenin and oleic acid identified as active compounds. Molecular docking confirmed strong interactions of these compounds with core PCOS-associated proteins, further supporting marjoram's potential in modulating PCOS symptoms.</p><p><strong>Conclusion: </strong>This study reveals that marjoram contains a diverse range of active compounds that can modulate crucial biochemical and histological markers related to PCOS. By combining serum pharmacochemistry with network pharmacology, the research highlights marjoram's potential as a natural supplement to help alleviate PCOS symptoms and slow the syndrome's progression. These findings support further investigation into marjoram's role as a complementary therapy for managing PCOS.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"67"},"PeriodicalIF":3.3,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11846456/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143472259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combining network pharmacology and molecular docking to explore the pharmacological mechanism of Codonopsis Radix.-Hedysarum Multijugum Maxim.-Atractylodes Macrocephala Koidz. in treating lung cancer.","authors":"Bin He, Jun Cui, Zengwang Zhang, Yongjun Zhang","doi":"10.1186/s12906-025-04823-z","DOIUrl":"10.1186/s12906-025-04823-z","url":null,"abstract":"<p><strong>Aim: </strong>To explore the potential mechanism of the treatment of lung cancer (LC) with Codonopsis Radix.-Hedysarum Multijugum Maxim.-Atractylodes Macrocephala Koidz. (CHA).</p><p><strong>Methods: </strong>The active ingredients and targets of CHA were obtained from TCMSP and SwissTargetPrediction databases. LC-related genes were obtained from MalaCards, GeneCards and DisGenNET databases. A protein-protein interaction network was constructed using STRING database, and analyzed with Cystoscape software. The core targets of CHA in LC treatment were determined by topological analysis, and functional annotation of these targets was performed via Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. The binding ability of the target to the active ingredient was evaluated by molecular docking. CCK-8 assay, Transwell assay, qRT-PCR, Western blot and lung metastasis model in nude mice were used to evaluate the effects of CHA on the viability, migration and invasion of LC cells.</p><p><strong>Results: </strong>27 active components and 411 common targets of CHA in lung cancer treatment were obtained. The common targets were associated multiple biological processes and pathways including PI3K-AKT pathway. 12-senecioyl-2E,8E,10E-atractylentriol, 14-acetyl-12-senecioyl-2E,8Z,10E-atractylentriol, astrapterocarpan, isoflavanone, (R)-Isomucronulatol were identified as the main bioactive components of CHA, and SRC, HSP90AA1, AKT1, EGFR, ESR1 were identified as core targets of CHA in LC treatment. The bioactive ingredients had good binding ability with the core targets. CHA significantly inhibited the viability, migration and invasion of LC cells, and also suppressed the transcription of core genes, and repressed the activation of PI3K/AKT pathway.</p><p><strong>Conclusion: </strong>For LC, CHA exerts tumor-suppressive effects through various bioactive components, acting on multiple targets and signaling pathways.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"66"},"PeriodicalIF":3.3,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11846413/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143472258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Liuzijue Qigong on respiratory function among patients with Parkinson's disease: a randomized clinical trial.","authors":"Huimei Yin, Oumei Cheng, Xu Zhang, Fengying Quan, Yujiao Zhang, Hongzhou Zuo, Jianrong Zhou, Shi-Qi Xie","doi":"10.1186/s12906-025-04773-6","DOIUrl":"10.1186/s12906-025-04773-6","url":null,"abstract":"<p><strong>Background: </strong>Respiratory dysfunction is a common nonmotor symptom in patients with Parkinson's disease that may be easily overlooked. Incorporating respiratory function rehabilitation into routine rehabilitation programs for these patients is important. However, previous studies have focused primarily on professional devices and training processes that require additional professional guidance, making wide implementation challenging.</p><p><strong>Methods: </strong>In this assessor-masked, randomized clinical trial, eligible participants were randomized into an experimental group and a control group. The experimental group received Liu Zi Jue Qigong exercise for 12 weeks, while the control group underwent conventional rehabilitation exercises. Primary outcomes included measures of respiratory function, such as forced vital capacity (FVC), forced expiratory volume in 1 s (FEV1), the ratio of forced expiratory volume in 1 s to forced vital capacity(FEV1/FVC), peak expiratory flow (PEF), maximal inspiratory pressure (MIP), and maximal expiratory pressure (MEP). Secondary outcomes included motor symptoms, quality of life, depression, and anxiety.</p><p><strong>Results: </strong>The results from a study involving 51 Parkinson's disease patients showed significant improvements in the FVC, FEV1, PEF, and MEP in the experimental group compared to those in the control group after the 12-week intervention. Additionally, there was a noticeable decrease in depression and anxiety scores in the experimental group, with statistically significant reductions compared to those in the control group.</p><p><strong>Conclusions: </strong>Liuzijue Qigong exercise has the potential to enhance respiratory function and mental health in patients with Parkinson's disease in the early and middle stages. These findings could serve as a valuable reference for implementing home-based rehabilitation techniques for individuals with Parkinson's disease.</p><p><strong>Trial registration: </strong>Chinese Clinical Trial Registry: ChiCTR2200056762(Retrospectively registered, Registration Date: February 15, 2022).</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"63"},"PeriodicalIF":3.3,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11843974/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of complementary medicines among older adults living in 23 residential aged care facilities in Australia.","authors":"Narjis Batool, Magdalena Z Raban, Karla L Seaman, Johanna I Westbrook, Nasir Wabe","doi":"10.1186/s12906-025-04811-3","DOIUrl":"10.1186/s12906-025-04811-3","url":null,"abstract":"<p><strong>Background: </strong>There is limited information on complementary medicine (CM) use among older adults living in residential aged care facilities (RACFs). This study aimed to determine the prevalence and predictors of CM use and to examine differences in CM use by facility for residents of RACFs.</p><p><strong>Methods: </strong>We conducted a retrospective study using routinely collected electronic data about permanent residents aged ≥ 65 years in December 2021 from 23 RACFs in Sydney. The prevalence of CM product use was estimated, and modelling was used to determine factors associated with CM use. Funnel plots visualised differences in prevalence of CM use between facilities.</p><p><strong>Results: </strong>A total of 1,873 residents were included in the analysis with 78.4% (95% CI: 76.5-80.3) using at least one CM product and 41.2% (95% CI: 39-43.4) using 2 or more CMs. The most frequently used CM products were vitamin D (61.4%), magnesium (18.0%) and calcium (13.1%). Certain diagnoses were associated with both the likelihood of using a CM as well as the number of CMs used. For example, individuals with fractures were more likely to use ≥ 2 CMs (OR 1.29; 95% CI 1.05-1.58), as were those with an endocrine disorder. Residents with circulatory conditions and dementia were less likely to use ≥ 2 CMs. The prevalence of residents using at least one CMs ranged from 54 to 88% between facilities.</p><p><strong>Conclusions: </strong>The prevalence of CM use is high in RACFs. Research to investigate the appropriateness of CM use to ensure their safe and effective use in RACFs is needed.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"64"},"PeriodicalIF":3.3,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11843739/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant and cytotoxic properties of protocorm-derived phenol-rich fractions of Dendrobium amoenum.","authors":"Mukti Ram Paudel, Sujata Sharma, Pusp Raj Joshi, Basant Pant, Sven H Wagner, Pritam Gurung, Krishna Kumar Pant, Bijaya Pant","doi":"10.1186/s12906-025-04810-4","DOIUrl":"10.1186/s12906-025-04810-4","url":null,"abstract":"<p><strong>Background: </strong>Dendrobium amoenum is known for its aesthetic and medicinal values but it is threatened due to loss of wild resources. Plant tissue culture promotes wild resource protection and paves the way for secondary metabolite production. In this study, protocorms developed via in-vitro seed cultivation were used for bioactive secondary metabolite production. The objectives of this study were to evaluate total phenolic and flavonoid contents, to identify the bioactive secondary metabolites, to explore the antioxidants and cytotoxic properties of in-vitro-derived protocorms extracts of D. amoenum.</p><p><strong>Methods: </strong>Seeds of D. amoenum were cultivated on 10% coconut water, 0.25 and 0.5 mg/L BAP supplemented full-strength and half-strength MS medium to produce protocorms for the isolation of bioactive components. A distinct yellow fraction (DAYF), light-green fraction (DALGF), green fraction (DAGF), and dark-green fraction (DADGF) were obtained from methanol extract on a methanol-based Sephadex LH-20 column. The total phenol and flavonoid contents along with the antioxidant and cytotoxic properties of the fractions were evaluated. The compounds in active DAYF were identified using a GC-MS.</p><p><strong>Results: </strong>On a full-strength solid MS medium supplemented with 10% coconut water, approximately 95% of the seeds grew into protocorms, while 88.33% did so on a full-strength liquid MS medium. The DAYF had a total phenol content of 206.38 μg of GAE and a total flavonoid content of 101.88 μg of QE. Owing to these high contents, the DAYF inhibited 50% of the DPPH free radicals at a concentration of 63.73 μg/ml. Similarly, it also reduced the growth of HeLa cells by 50% at 67.03 μg/ml and U2OS cells by 50% at 207.40 μg/ml, while it was nontoxic to normal human epithelium cells. Bioactive phenolic compounds 2-methoxy-4-vinylphenol (1), 3,4-dimethoxy-phenol (2), 2-methoxy-4-(1-propenyl)-phenol (3), 2,6-dimethoxy-4-(2-propenyl)-phenol (4), 3-methoxy-1,2-benzenediol (5) were identified in the DAYF.</p><p><strong>Conclusion: </strong>Protocorms of D. amoenum could serve as sources of bioactive secondary metabolites highlighting their potential in alternative medicine.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"61"},"PeriodicalIF":3.3,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11837413/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143448073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical and antibacterial properties of calyces Hibiscus sabdariffa L.: an in vitro and in silico multitarget-mediated antibacterial study.","authors":"Hend Khairy Fekry Ghaly, Fatema Aly Al-Yamany Younis, Azza Mahmoud Soliman, Sabha Mahmoud El-Sabbagh","doi":"10.1186/s12906-025-04794-1","DOIUrl":"10.1186/s12906-025-04794-1","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Multidrug-resistant (MDR) bacteria pose a significant threat to human health worldwide by increasing the harmful impact of traditional synthetic antibiotics. Traditional medicinal plants have bioactive metabolites that can significantly modulate the growth rate, cell survival, and pathogenicity of antibiotic-resistant bacteria. Hibiscus sabdariffa L., known as Roselle or Karkade, belongs to the Malvaceae family. It is well-known for its edible aromatic red/purple calyces and is extensively utilized in the food industry and pharmacological applications. H. sabdariffa calyx bioactive phytocompounds have potent therapeutic activities such as antimicrobial, antidiabetic, antiobesity, antioxidant, anti-inflammatory, and anticancer properties.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;This study utilized gas chromatography-mass spectrometry (GC-MS) analysis to determine the volatile aromatic compounds that found in the hydroethanolic extract of Hibiscus sabdariffa calyces. The purpose was to verify the antibacterial properties of Roselle calyces against selective MDR clinical bacterial isolates, including A. baumanii, E. coli, K. pneumoniae, and P. aeruginosa.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;The GC-MS spectrum profile revealed the presence of twenty-seven volatile organic components, including organic fatty acid derivatives, ester compounds, sugar derivatives, and terpene components. The major GC-MS fractionations and the main active chemical compositions of the hydroethanolic extract of H. sabdariffa flowers were (E)-10-Octadecenoic acid methyl ester (59.23%), 8,11-Octadecadienoic acid, methyl ester (11.51%), Butanedioic acid, 3-hydroxy-2,2-dimethyl-, diethyl ester (6.22%), Diethyl succinate/Butanedioic acid, diethyl ester (2.35%), and Heptadecanoic acid, 16-methyl-, methyl ester/Methyl isostearate (2.31%). The hydroethanolic extract of H. sabdariffa dried calyces demonstrated potent antibacterial properties (zones diameter of inhibition growth, MIC, MBC, and MBC/MIC) against selective MDR clinical bacterial isolates, such as A. baumanii, E. coli, K. pneumoniae, and P. aeruginosa, as determined by the phytochemical screening (TAC, TFC, and TPC) and antioxidant activity (DPPH). The surface morphological characteristics of the treated A. baumanii, E. coli, K. pneumoniae, and P. aeruginosa clinical isolates have been affected in comparison to the untreated forms by the hydroethanolic extract of H. sabdariffa calyces, as determined by scanning electron microscopy (SEM). In silico predictive investigation revealed that the volatile aromatic components of the hydroethanolic extract of Roselle calyces exhibited significant scoring functions, binding affinities, and non-covalent intermolecular interactions with the MenB lyase and DNA gyrase targets of E. coli. These interactions significantly enhanced the activities of the volatile aromatic components against the bacterial pathogenicity, cell survival, growth, and differentiation of sele","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"62"},"PeriodicalIF":3.3,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11837655/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143447618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial Expression of Concern: The aqueous extract of Artemisia Absinthium L. stimulates HO-1/MT-1/Cyp450 signaling pathway via oxidative stress regulation induced by aluminium oxide nanoparticles (α and γ) animal model.","authors":"Esmaeil Karami, Zahra Goodarzi, Seyed Jamaleddin Shahtaheri, Mehrafarin Kiani, Mohammad Faridan, Mahmoud Ghazi-Khansari","doi":"10.1186/s12906-025-04821-1","DOIUrl":"10.1186/s12906-025-04821-1","url":null,"abstract":"","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"60"},"PeriodicalIF":3.3,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11834540/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytotoxic and immunomodulatory properties of Tinospora cordifolia, Boerhaavia diffusa, Berberis aristata, and Ocimum basilicum extracts against HPV-positive cervical cancer cell line.","authors":"Harsha Vaghasia, Rahul Patel, Jignesh Prajapati, Krupa Shah, Meenu Saraf, Rakesh M Rawal","doi":"10.1186/s12906-025-04817-x","DOIUrl":"10.1186/s12906-025-04817-x","url":null,"abstract":"<p><strong>Background: </strong>Cervical cancer remains a significant public health concern, especially for low-and middle-income countries. This study explores the dual potential of medicinal plant extracts as both anticancer agents and immunomodulators, particularly in HPV-positive cervical cancer, while also addressing the limitations of conventional chemotherapy.</p><p><strong>Methods: </strong>Extracts from Tinospora cordifolia, Boerhaavia diffusa, Berberis aristata, and Ocimum basilicum were tested on CaSki and HEK 293 cells alongside cisplatin. Cytotoxicity, genotoxicity, cell migration, HPV DNA inhibition, IFNG secretion, and cell cycle modulation were assessed using established biochemical and immunological assays, including qPCR, ELISA, and flow cytometry.</p><p><strong>Results: </strong>Among the extracts, B. aristata demonstrated the strongest anticancer effects on cancer cells while exhibiting minimal impact on normal cells, highlighting its therapeutic potential. It also significantly reduced cell migration and has synergistic affect with cisplatin. B. diffusa emerged as the most effective in inhibiting HPV 16 DNA, suggesting its role in viral suppression. Immunomodulatory analysis revealed that T. cordifolia and O. basilicum significantly enhanced IFNG secretion, indicative of robust immune activation. Each tested extracts induced G0/G1 phase cell cycle arrest, with T. cordifolia showing the most pronounced effect.</p><p><strong>Conclusions: </strong>This study highlights the novel therapeutic promise of integrating plant extracts into HPV-positive cervical cancer management. B. aristata stands out for its cytotoxicity and anti-migration properties, while T. cordifolia offers significant immunomodulatory benefits. These findings pave the way for further research into combining natural products with conventional therapies for safer, more effective cancer treatments.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"25 1","pages":"59"},"PeriodicalIF":3.3,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11834557/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}