Chromatography (Basel)最新文献_第7页

UV Cut-Off Filter of a Photodiode Array Detector Improves the Quantitativity of L-Ascorbic Acid Through Its Photoprotection 光电二极管阵列检测器的紫外截止滤波器通过其光保护提高了l -抗坏血酸的定量

Chromatography (Basel) Pub Date : 2020-10-20 DOI: 10.15583/jpchrom.2020.013

Kohei Kawabata, Takato Uchikata, Keiko Matsumoto, H. Nishi

{"title":"UV Cut-Off Filter of a Photodiode Array Detector Improves the Quantitativity of L-Ascorbic Acid Through Its Photoprotection","authors":"Kohei Kawabata, Takato Uchikata, Keiko Matsumoto, H. Nishi","doi":"10.15583/jpchrom.2020.013","DOIUrl":"https://doi.org/10.15583/jpchrom.2020.013","url":null,"abstract":"A photodiode array detector (PDA) is frequently utilized as a detector in high-performance liquid chromatography (HPLC) system for a detection of various compounds. A PDA emits a light of a wide range wavelength including ultraviolet light (UV) which has a possibility to induce photodegradation of analyzed compounds. If so, target compounds might be degraded when analyzed in this HPLC system. Therefore, photoprotection during HPLC analysis is required for the accurate analysis. In this study, the protective effect of a UV cut-off filter, which can cut off UV especially at shorter wavelength (< 240 nm), on the quantitativity of a photodegradable compound L -ascorbic acid (AA) was examined. A UV cut-off filter prevents AA from photodegradation, followed by the improvement of several parameters of a calibration curve. Furthermore, this protective potency was significant in the case that photodegradability of AA was enhanced with the conditions such as a small injection volume and a low flow rate. This study strongly suggests that the UV cut-off filter is a useful equipment when analyzing photodegradable compounds.","PeriodicalId":91226,"journal":{"name":"Chromatography (Basel)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42030928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescence Bioanalysis of Bevacizumab Using Pre-Column and Post-Column Derivatization – Liquid Chromatography After Immunoaffinity Magnetic Purification Bevacizumab的柱前和柱后衍生荧光生物分析——免疫亲和磁纯化后的液相色谱法

Chromatography (Basel) Pub Date : 2020-10-20 DOI: 10.15583/jpchrom.2020.014

K. Todoroki, Tatsuki Nakano, H. Hayashi, H. Mizuno, J. Min, T. Toyo’oka

{"title":"Fluorescence Bioanalysis of Bevacizumab Using Pre-Column and Post-Column Derivatization – Liquid Chromatography After Immunoaffinity Magnetic Purification","authors":"K. Todoroki, Tatsuki Nakano, H. Hayashi, H. Mizuno, J. Min, T. Toyo’oka","doi":"10.15583/jpchrom.2020.014","DOIUrl":"https://doi.org/10.15583/jpchrom.2020.014","url":null,"abstract":"This report presents two fluorescence labeling methods for therapeutic monoclonal antibody, bevacizumab, to increase its detection sensitivity for fluorescence detection. One method is high-temperature reversed-phase LC (HT-RPLC) following post-column fluorogenic derivatization using o -phthalaldehyde with thiol. Another method is pre-column derivatization using Zenon Alexa Fluor 488 protein-tag following size-exclusion chromatography (SEC). The calibration curves of bevacizumab were 1–50 μg/mL (post-column method) and 0.1–10 μg/mL (pre-column method). Both methods showed good correlation coefficients (r 2 > 0.991). The LOD and the LOQ of bevacizumab were, respectively, 0.13 and 0.43 μg/mL (post-column method) and 0.03 and 0.1 μg/mL (pre-column method). The sensitivities were about 2 and 10 times higher than that of native fluorescence detection. The proposed methods were applied to bevacizumab spiked human plasma samples. The bevacizumab in plasma samples was purified selectively with immunoaffinity beads and detected as a single peak using HT-RPLC or SEC with fluorescence detection.","PeriodicalId":91226,"journal":{"name":"Chromatography (Basel)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42272181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Improved Extraction Method for Catecholamines Using Monolithic Silica Disk-Packed Spin Column 整体硅胶圆盘填料旋转柱萃取儿茶酚胺方法的改进

Chromatography (Basel) Pub Date : 2020-10-20 DOI: 10.15583/jpchrom.2020.012

Takuya Fujiwara, T. Funatsu, M. Tsunoda

引用次数: 2

Sensitive Method for LC Analysis of Therapeutic Monoclonal Antibodies Using a Centrifugal Filtration Device with Adsorption Suppression Treatment 采用吸附抑制处理的离心过滤装置进行治疗性单克隆抗体LC分析的灵敏方法

Chromatography (Basel) Pub Date : 2020-10-20 DOI: 10.15583/jpchrom.2020.016

Y. Shibata, Tomohiro Yamada, Eiji Sugiyama, H. Mizuno, K. Todoroki

引用次数: 1

Ultralow-Temperature HPLC Using Low-Molecular-Weight Hydrocarbons as Mobile Phases 以低分子量碳氢化合物为流动相的超低温高效液相色谱法

Chromatography (Basel) Pub Date : 2020-10-20 DOI: 10.15583/jpchrom.2020.011

Tomohiro Motono, Takayuki Kanayama, S. Kitagawa, Yoshinori Iiguni, H. Ohtani

引用次数: 1

Retention Behavior of Various Aromatic Compounds on Poly(butylene terephthalate) Stationary Phase in Liquid Chromatography 不同芳香族化合物在聚对苯二甲酸丁二酯固定相上的液相色谱保留行为

Chromatography (Basel) Pub Date : 2020-10-20 DOI: 10.15583/jpchrom.2020.017

Koki Nakagami, Misato Amiya, K. Shimizu, Ohjiro Sumiya, Ryota Koike, I. Ueta, Yoshihiro Saito

{"title":"Retention Behavior of Various Aromatic Compounds on Poly(butylene terephthalate) Stationary Phase in Liquid Chromatography","authors":"Koki Nakagami, Misato Amiya, K. Shimizu, Ohjiro Sumiya, Ryota Koike, I. Ueta, Yoshihiro Saito","doi":"10.15583/jpchrom.2020.017","DOIUrl":"https://doi.org/10.15583/jpchrom.2020.017","url":null,"abstract":"Poly(butylene terephthalate)-coated silica (PBT) have been introduced as a stationary phase in liquid chromatography (LC) and the retention behavior of polycyclic aromatic compounds (PACs) was evaluated in reversed-phase LC. The trend for the retention was compared with that obtained on two types of commercially-available octadecylsilica (ODS) phases and phenylbutylsilica (PBS) phase. A good liner relationship between molecular size of planar PACs and the corresponding logarithmic retention factor was confirmed on the PBT stationary phase, and the trend is quite similar to that obtained on a conventional polymeric ODS stationary phase. In addition, a good molecular shape recognition capability of the PBT stationary phase was confirmed for several solute pairs consisted of planar and non-planar PACs with a similar two-dimensional molecular size. The selectivities to some planar/non-planar solute pairs on the PBT stationary phase were significantly better than conventional ODS phases, even when compared with that of typical polymeric ODS stationary phases operated in a similar experimental condition. In the case of structural isomers of dichlorobenzene and dibromobenzene, the elution order on the PBT stationary phase was o-, mand p-, however the corresponding elution order in typical ODS phases, and PBS phase was different, o-, pand m-. The results can be explained on the basis of the molecular-molecular interaction between the stationary phase ligand and the analyte molecule, because the PBT stationary phase has a similar partial chemical structure to these p-isomers on the silica support.","PeriodicalId":91226,"journal":{"name":"Chromatography (Basel)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67104157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

High-Performance Liquid Chromatographic Determination of Chiral Amino Acids Using Pre-Column Derivatization with o-Phthalaldehyde and N-tert-Butyloxycarbonyl-D-cysteine and Application to Vinegar Samples 邻苯二甲醛和N-叔丁氧羰基-D-半胱氨酸柱前衍生高效液相色谱法测定手性氨基酸及其在醋中的应用

Chromatography (Basel) Pub Date : 2020-10-20 DOI: 10.15583/jpchrom.2020.015

Aogu Furusho, Mintranee Obromsuk, T. Akita, M. Mita, M. Nagano, P. Rojsitthisak, K. Hamase

{"title":"High-Performance Liquid Chromatographic Determination of Chiral Amino Acids Using Pre-Column Derivatization with o-Phthalaldehyde and N-tert-Butyloxycarbonyl-D-cysteine and Application to Vinegar Samples","authors":"Aogu Furusho, Mintranee Obromsuk, T. Akita, M. Mita, M. Nagano, P. Rojsitthisak, K. Hamase","doi":"10.15583/jpchrom.2020.015","DOIUrl":"https://doi.org/10.15583/jpchrom.2020.015","url":null,"abstract":"A reversed-phase high-performance liquid chromatographic (HPLC) method using pre-column derivatization with o -phthalaldehyde (OPA) plus N - tert -butyloxycarbonyl- D -cysteine (Boc- D -Cys) has been developed for the determination of aspartic acid (Asp), serine (Ser) and alanine (Ala) enantiomers. D -Amino acids in the real world samples are trace in most cases, and their small peaks should be eluted faster than the huge peaks of the L -forms in order to avoid overlapping. Amino acids were rapidly derivatized at room temperature with OPA plus Boc- D -Cys under simple conditions and were detected by their fluorescence. The target amino acid enantiomers were separated within 60 min on a reversed-phase column, CAPCELL PAK C18 MG II (4.6 x 200 mm), and their resolution values were higher than 2.14. The developed system was successfully validated using standard amino acids, and sufficient calibration lines ( r 2 > 0.9983) and precision (RSD < 5.29%) results were obtained. In a Japanese traditionally fermented amber rice vinegar, all of the target D -amino acids were observed, and their % D values were 21.5 for Asp, 6.8 for Ser and 22.9 for Ala.","PeriodicalId":91226,"journal":{"name":"Chromatography (Basel)","volume":" 46","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41254177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Simultaneous Determination of 7 Short-Chain Fatty Acids in Human Saliva by High-Sensitivity Gas Chromatography-Mass Spectrometry 高灵敏度气相色谱-质谱法同时测定人唾液中的7种短链脂肪酸

Chromatography (Basel) Pub Date : 2020-06-20 DOI: 10.15583/jpchrom.2019.025

Takahiro Kawase, K. Hatanaka, M. Kono, Y. Shirahase, K. Ochiai, S. Takashiba, T. Tsukahara

{"title":"Simultaneous Determination of 7 Short-Chain Fatty Acids in Human Saliva by High-Sensitivity Gas Chromatography-Mass Spectrometry","authors":"Takahiro Kawase, K. Hatanaka, M. Kono, Y. Shirahase, K. Ochiai, S. Takashiba, T. Tsukahara","doi":"10.15583/jpchrom.2019.025","DOIUrl":"https://doi.org/10.15583/jpchrom.2019.025","url":null,"abstract":"Upon absorption in the intestine of the host animal, the main function of short-chain fatty acids (SCFAs), mainly acetate, propionate and n-butyrate, is as metabolic energy. SCFAs, n-butyrate in particular, can also be found in the mouth. An excess of oral SCFAs may cause not only periodontal diseases but also systemic abnormalities in humans. Previously, we reported a method for simultaneous detection by gas chromatography-mass spectrometry (GC-MS) of acetate, propionate and n-butyrate in serum, urine and saliva. In the present study we used a modified version of this method to detect not only acetate, propionate and n-butyrate, but also iso-butyrate, n-valerate, iso-valerate and caproate, because the latter are suggested to be associated with periodontal diseases. Detection ranges of SCFAs were as follows; 6.25-400 µmol/L (acetate), 0.781-100 µmol/L (propionate and n-butyrate), 0.391-50 µmol/L (iso-butyrate), 0.781-50 µmol/L (n-valerate and iso-valerate) and 1.56-50 µmol/L (caproate). Furthermore, we validated the modified detection method with triple freeze-thaw-cycle recovery tests and intra- and inter-day repeatability. Freezing and thawing did not influence the concentrations of SCFAs in saliva. Upon analysis of five clinical saliva samples, it was observed that, except for n-valerate, which was detected only in two samples, all SCFAs were detected in saliva samples. To conclude, we were able to use a modified method to analyze successfully by GC-MS the salivary concentrations of SCFAs. In addition, we simultaneously detected the salivary concentrations of iso-butyrate, iso-valerate, n-valerate and caproate. This improved method was proved to be reliable to measure the concentrations of SCFAs in saliva.","PeriodicalId":91226,"journal":{"name":"Chromatography (Basel)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.15583/jpchrom.2019.025","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47921694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Simple Simultaneous Assay of Methotrexate and Non-Steroidal Anti-Inflammatory Drugs by HPLC 高效液相色谱法同时测定甲氨蝶呤和非甾体类抗炎药的含量

Chromatography (Basel) Pub Date : 2020-06-20 DOI: 10.15583/jpchrom.2020.009

Makoto Takada, Y. Ohba, T. Kabashima, K. Nakashima, M. Wada

{"title":"Simple Simultaneous Assay of Methotrexate and Non-Steroidal Anti-Inflammatory Drugs by HPLC","authors":"Makoto Takada, Y. Ohba, T. Kabashima, K. Nakashima, M. Wada","doi":"10.15583/jpchrom.2020.009","DOIUrl":"https://doi.org/10.15583/jpchrom.2020.009","url":null,"abstract":"The combination of methotrexate (MTX) and non-steroidal anti-inflammatory drugs (NSAIDs), which is frequently used for rheumatoid arthritis (RA) treatment, courses of adverse events. To prevent these, simultaneous monitoring of these compounds is available. Therefore, we developed a new method for MTX and NSAIDs such as loxoprofen (LP), meloxicam (MX), lornoxicam (LX), diclofenac (DF) and celecoxib (CX) determination using HPLC with simple pretreatment of human serum sample. The separation of MTX and 5 NSAIDs was performed on C6-Phenyl column with gradient elution using 10 mmol/L ammonium acetate aqueous solution and methanol, and achieved within 25 min of analytical runtime. Calibration curves using standards showed good linearity ( r 2 >0.9998) in the range of 0.02-500 pmol/10 μL injection for MTX, 10-500 pmol for LP, 0.2-500 pmol for MX and DF, 0.05-500 pmol for LX and 2-500 pmol for CX. The detection limits of the proposed method were at least less than 96.0 fmol, and repeatability was less than 4.62 RSD%. In addition, acceptable precision of less than 10.19 RSD% and recovery of more than 54.4% of the method were obtained because the peaks of MTX and NSAIDs could be well-separated from those of interferings in serum. the method be useful to avoid occurring adverse events, and confirm the curative effect of MTX and NSAIDs.","PeriodicalId":91226,"journal":{"name":"Chromatography (Basel)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45212864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Capillary Electrophoretic Characterization of Carbon Nanodots Prepared from Glutamic Acid in an Electric Furnace 电炉中谷氨酸制备碳纳米点的毛细管电泳表征

Chromatography (Basel) Pub Date : 2020-06-20 DOI: 10.15583/jpchrom.2020.010

T. Takayanagi, S. Iwasaki, Kotaro Morita, N. Hirayama, H. Mizuguchi

引用次数: 1