Chromatography (Basel)最新文献_第6页

Separation of Dansyl-DL-Amino Acids Through Tube Radial Distribution Chromatography by Using a Commercially Available HPLC System with a Capillary Tube Manufactured for GC as a Separation Column 以气相色谱用毛细管为分离柱，利用市售的高效液相色谱系统对丹酚- dl -氨基酸进行管状径向分布色谱分离

Chromatography (Basel) Pub Date : 2021-02-20 DOI: 10.15583/JPCHROM.2020.019

Nobuyuki Sanada, K. Tsukagoshi, K. Tsuchiya, K. Hirota, K. Yamashita, M. Murata

{"title":"Separation of Dansyl-DL-Amino Acids Through Tube Radial Distribution Chromatography by Using a Commercially Available HPLC System with a Capillary Tube Manufactured for GC as a Separation Column","authors":"Nobuyuki Sanada, K. Tsukagoshi, K. Tsuchiya, K. Hirota, K. Yamashita, M. Murata","doi":"10.15583/JPCHROM.2020.019","DOIUrl":"https://doi.org/10.15583/JPCHROM.2020.019","url":null,"abstract":"Dansyl- DL -amino acids were separated by tube radial distribution chromatography (TRDC) comprising a commercially available HPLC system, where open-tubular capillary tubes manufactured for capillary GC were used as the separation column. Initially, the separation performance of GC capillary tubes in TRDC was assessed by separating a model analyte mix of 1-naphthol and 2,6-naphthalenedisulfonic acid by using water/acetonitrile/ethyl acetate solutions (3:8:4 volume ratio, organic solvent-rich and 4:3:1 volume ratio, water-rich) as the eluent and high-polarity and non-polarity capillary tubes. Next, enantiomer separation of dansyl- DL -methionine and dansyl- DL -valine was examined using a water/acetonitrile/ethyl acetate solution (8:2:1 volume ratio, water-rich) containing 1.0 mM  -cyclodextrin and non-polarity capillary tubing. The D - and L -enantiomers were separated and detected in this order through interaction between amino acids and  -cyclodextrin by tube radial distribution flow in the TRDC system.","PeriodicalId":91226,"journal":{"name":"Chromatography (Basel)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48189952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effect of Organic Modifier on the Retention of Low-Molecular-Weight Organic Compounds in Low-Temperature HPLC Using a Liquid CO2 Mobile Phase and an Octadecyl Stationary Phase 采用液态CO2流动相和十八烷基固定相低温高效液相色谱研究有机改性剂对低分子量有机化合物保留的影响

Chromatography (Basel) Pub Date : 2021-02-20 DOI: 10.15583/JPCHROM.2020.025

K. Matsuo, Mina Otsubo, Tomohiro Motono, S. Kitagawa, Yoshinori Iiguni, H. Ohtani

{"title":"Effect of Organic Modifier on the Retention of Low-Molecular-Weight Organic Compounds in Low-Temperature HPLC Using a Liquid CO2 Mobile Phase and an Octadecyl Stationary Phase","authors":"K. Matsuo, Mina Otsubo, Tomohiro Motono, S. Kitagawa, Yoshinori Iiguni, H. Ohtani","doi":"10.15583/JPCHROM.2020.025","DOIUrl":"https://doi.org/10.15583/JPCHROM.2020.025","url":null,"abstract":"The retention behaviors of various low-molecular-weight compounds in low-temperature HPLC with an ODS (octadecyl silica) column and liquid CO 2 mobile phase containing methanol or acetonitrile as modifiers were investigated. The addition of methanol/acetonitrile reduced the retention of xanthone, which was dominated by the liquid CO 2 content, independent of the modifier. Additionally, the retention of polycyclic aromatic hydrocarbons (PAHs) was reduced by the addition of methanol/acetonitrile. Because acetonitrile resulted in a rapid reduction in the retention compared with methanol, it was suggested that the CH-  interactions have an important role in the retention of PAHs in the C18 stationary phase. Notably, the retention of alkylbenzenes were only slightly affected by the addition of methanol/acetonitrile. Acetonitrile slightly increased the retention of alkylbenzenes with longer alkyl chains than those with shorter alkyl chains. It is suggested that the interaction of the phenyl moiety in alkylbenzenes with C18 chains was reduced by the increase in the acetonitrile content, and enhanced in the case of the alkyl chain moieties. The retentions of alkyl benzenes were approximately compensated, when the alkyl chain length was relatively","PeriodicalId":91226,"journal":{"name":"Chromatography (Basel)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49241692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Determination of Two-Steps Acid Dissociation Constants of L-Ascorbic Acid by Capillary Zone Electrophoresis 毛细管区带电泳法测定l -抗坏血酸两步酸解离常数

Chromatography (Basel) Pub Date : 2021-02-20 DOI: 10.15583/JPCHROM.2020.024

Yuki Tanikami, H. Mizuguchi, T. Takayanagi

{"title":"Determination of Two-Steps Acid Dissociation Constants of L-Ascorbic Acid by Capillary Zone Electrophoresis","authors":"Yuki Tanikami, H. Mizuguchi, T. Takayanagi","doi":"10.15583/JPCHROM.2020.024","DOIUrl":"https://doi.org/10.15583/JPCHROM.2020.024","url":null,"abstract":"Two-steps acid dissociation constants of L-ascorbic acid (AA) were determined through the changes in the effective electrophoretic mobility in capillary zone electrophoresis. Although ascorbic acid is oxidatively degradable in an aqueous solution, especially in alkaline conditions, the effective electrophoretic mobility of AA was successfully measured in the pH range between 1.87 and 11.97. In the analysis of the first-step acid dissociation constant (pKa1) at weakly acidic pH conditions, a coated capillary with 1,3-propanesultone and a pressure-assist were utilized to detect anionic AA. In the analysis of the second-step acid dissociation constant (pKa2) at weakly alkaline pH conditions, AA was successfully detected at the pH range up to 11.97 with the help of Cu catalyst to remove the dissolved oxygen in the separation buffer. Acid dissociation constants were independently determined as pKa1 = 4.15±0.01 and pKa2 = 12.07±0.04 by non-linear least-squares analyses. AA did not fully dissociate at the weakly alkaline pH range, and the effective electrophoretic mobility of the dianion form of AA was extrapolated for the analysis.","PeriodicalId":91226,"journal":{"name":"Chromatography (Basel)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43056873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular design revitalizes the low-cost PTV-polymer for highly efficient organic solar cells. 分子设计使低成本 PTV 聚合物重新焕发活力，可用于高效有机太阳能电池。

IF 16.3

Chromatography (Basel) Pub Date : 2021-02-12 eCollection Date: 2021-08-01 DOI: 10.1093/nsr/nwab031

Junzhen Ren, Pengqing Bi, Jianqi Zhang, Jiao Liu, Jingwen Wang, Ye Xu, Zhixiang Wei, Shaoqing Zhang, Jianhui Hou

{"title":"Molecular design revitalizes the low-cost PTV-polymer for highly efficient organic solar cells.","authors":"Junzhen Ren, Pengqing Bi, Jianqi Zhang, Jiao Liu, Jingwen Wang, Ye Xu, Zhixiang Wei, Shaoqing Zhang, Jianhui Hou","doi":"10.1093/nsr/nwab031","DOIUrl":"10.1093/nsr/nwab031","url":null,"abstract":"Developing photovoltaic materials with simple chemical structures and easy synthesis still remains a major challenge in the industrialization process of organic solar cells (OSCs). Herein, an ester substituted poly(thiophene vinylene) derivative, PTVT-T, was designed and synthesized in very few steps by adopting commercially available raw materials. The ester groups on the thiophene units enable PTVT-T to have a planar and stable conformation. Moreover, PTVT-T presents a wide absorption band and strong aggregation effect in solution, which are the key characteristics needed to realize high performance in non-fullerene-acceptor (NFA)-based OSCs. We then prepared OSCs by blending PTVT-T with three representative fullerene- and NF-based acceptors, PC71BM, IT-4F and BTP-eC9. It was found that PTVT-T can work well with all the acceptors, showing great potential to match new emerging NFAs. Particularly, a remarkable power conversion efficiency of 16.20% is achieved in a PTVT-T:BTP-eC9-based device, which is the highest value among the counterparts based on PTV derivatives. This work demonstrates that PTVT-T shows great potential for the future commercialization of OSCs.","PeriodicalId":91226,"journal":{"name":"Chromatography (Basel)","volume":"41 1","pages":"nwab031"},"PeriodicalIF":16.3,"publicationDate":"2021-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8966978/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82350041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Precolumn Derivatization–LC/MS for Amino-Acid-Focused Metabolomics 以氨基酸为中心的代谢组学柱前衍生- lc /MS的发展

Chromatography (Basel) Pub Date : 2021-02-07 DOI: 10.15583/JPCHROM.2020.023

H. Miyano, Akira Nakayama

{"title":"Development of Precolumn Derivatization–LC/MS for Amino-Acid-Focused Metabolomics","authors":"H. Miyano, Akira Nakayama","doi":"10.15583/JPCHROM.2020.023","DOIUrl":"https://doi.org/10.15583/JPCHROM.2020.023","url":null,"abstract":"Focused metabolomics facilitates the understanding of the current phenotype of living organisms, involves highly practical methods for investigation, and allows for the acquisition of highly quantitative data for important endogenous metabolites. The authors developed novel precolumn derivatization reagents for the analysis of amino acids, which are particularly important endogenous metabolites, through precolumn liquid chromatography tandem mass spectrometry (LC-MS/MS). One of the reagents facilitated sub-femtomole-to-attomole-scale amino acid detection. We also developed an automated precolumn derivatization amino acid analyzer using another reagent for liquid chromatography–mass spectrometry (LC/MS). With this device, nearly 40 amino acids can be analyzed within a short time (<10 min), compared with the time (~2 h) taken by a conventional automated postcolumn derivatization amino acid analyzer. For highly accurate and precise analysis of free amino acid concentration in human plasma, we validated all steps, from blood collection to measurement, by using the proposed analyzer and developed a test procedure for studying free amino acid metabolomics in human plasma. While calculating the reference intervals of plasma amino acid concentrations in healthy individuals, it was found that the balance of amino acid concentrations in plasma is useful for risk screening of diseases including cancer, diabetes, and myocardial infarction. This functional-group-targeted analysis through LC/MS for amino acid was also effective for dipeptide, tripeptide, and D, L-amino acid analyses and amino acid detection in tissue by imaging mass spectrometry.","PeriodicalId":91226,"journal":{"name":"Chromatography (Basel)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46772546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Development of Fast and Selective Analytical Methods of Pharmaceuticals and Herbal Medicines by High-Performance Liquid Chromatography and Capillary Electrophoresis 高效液相色谱和毛细管电泳快速、选择性分析药物和草药方法的建立

Chromatography (Basel) Pub Date : 2021-02-06 DOI: 10.15583/JPCHROM.2020.026

H. Nishi

{"title":"Development of Fast and Selective Analytical Methods of Pharmaceuticals and Herbal Medicines by High-Performance Liquid Chromatography and Capillary Electrophoresis","authors":"H. Nishi","doi":"10.15583/JPCHROM.2020.026","DOIUrl":"https://doi.org/10.15583/JPCHROM.2020.026","url":null,"abstract":"Pharmaceuticals are essential for the medical treatment of various diseases. From the herbal medicines (crude drugs), synthetic medicines to biopharmaceuticals, to assure its efficacy and safety, the production procedures and the quality control methods of pharmaceuticals are important. These are strictly regulated by Good Manufacturing Practice (GMP) and various guidelines. The key is to develop the “Specifications and testing methods” of the pharmaceutical scientifically and rationally according to the production process. There are many testing items such as identity tests, purity tests, assay, etc. in “Specifications and testing methods”. Among them, assay and purity test are core testing items. Optical purity evaluation is required for the single enantiomer pharmaceutical. Chromatographic methods and its detection methods are useful for the purpose. High-performance liquid chromatography (HPLC), thin layer chromatography (TLC) and gas chromatography (GC) are commonly used because the target analyses are almost a mixture. In this review, results of the development of the quality evaluation methods of relatively low molecular pharmaceuticals and herbal medicines by HPLC and capillary electrophoresis (CE) we have been investigated are summarized.","PeriodicalId":91226,"journal":{"name":"Chromatography (Basel)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48713145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Sensitive and Selective LC Determination of 5-Hydroxyindoles Through Online Electrochemical Fluorescence Derivatization Using Benzylamine 苯胺在线电化学荧光衍生高效液相色谱法灵敏选择性测定5-羟基吲哚

Chromatography (Basel) Pub Date : 2021-02-02 DOI: 10.15583/JPCHROM.2020.022

K. Todoroki, Masaki Nakano, H. Yoshida, H. Nohta, M. Yamaguchi

引用次数: 1

Determination of Clenbuterol in Various Edible Parts of Livestock Products by LC-MS/MS and LC-MS/MS/MS Methods LC-MS/MS和LC-MS/MS法测定畜产品不同食用部位中的克伦特罗

Chromatography (Basel) Pub Date : 2021-01-22 DOI: 10.15583/JPCHROM.2020.021

Takako Hayashi, K. Hamase

引用次数: 0

In Situ Photopolymerization of Functionalized Polyacrylamide-Based Preconcentrators for Highly Sensitive Specific Detection of Various Analytes by Microchip Electrophoresis 功能化聚丙烯酰胺预富集剂的原位光聚合用于微芯片电泳对各种分析物的高灵敏度特异性检测

Chromatography (Basel) Pub Date : 2020-12-11 DOI: 10.15583/jpchrom.2020.020

Sachio Yamamoto

引用次数: 3

Microchip Electrophoresis Utilizing In Situ Photopolymerized Thrombin-Immobilized Preconcentrator Gels for Specific Entrapment and Analysis of Thrombin Aptamers 利用原位光聚合凝血酶固定化预浓缩凝胶进行凝血酶适体特异性包封和分析的微芯片电泳

Chromatography (Basel) Pub Date : 2020-12-07 DOI: 10.15583/jpchrom.2020.018

Sachio Yamamoto, Yume Kawaguchi, M. Kinoshita, Shigeo Suzuki

{"title":"Microchip Electrophoresis Utilizing In Situ Photopolymerized Thrombin-Immobilized Preconcentrator Gels for Specific Entrapment and Analysis of Thrombin Aptamers","authors":"Sachio Yamamoto, Yume Kawaguchi, M. Kinoshita, Shigeo Suzuki","doi":"10.15583/jpchrom.2020.018","DOIUrl":"https://doi.org/10.15583/jpchrom.2020.018","url":null,"abstract":"A method was developed for the specific entrapment and separation of thrombin aptamers using a thrombin-immobilized polyacrylamide gel fabricated at the channel crossing point of a microfluidic electrophoresis chip. The channel intersection of the poly(methyl methacrylate) (PMMA) microchip was filled with a solution comprising thrombin, acrylamide, N,N -methylene-bis-acrylamide, and 2,2’-azobis[2-methyl- N -(2-hydroxyethyl)propionamide], which functioned as a photocatalytic initiator. In situ polymerization at the channel crossing point was performed by irradiation with an LED laser beam. The fabricated thrombin-immobilized gel (100 ×100 × 30 µm) contained approximately 40 fmol of thrombin and therefore could entrap thrombin aptamers at the femtomolar level. The electrophoretically trapped thrombin aptamers were released from the gel by switching the voltage, which delivered high concentrations of phosphate ions in a background electrolyte. The broad sample band eluted from the gel was effectively reconcentrated at the boundary of a pH junction generated by sodium ions delivered from the outlet reservoir. The reconcentrated sample components were then separated and fluorometrically detected at the end of the separation channel. Under the optimized conditions, the thrombin aptamers were concentrated by a factor of 1,000-fold, and the peak resolution was comparable to that obtained by pinched injection. This method was successfully utilized to preconcentrate and analyze thrombin aptamers.","PeriodicalId":91226,"journal":{"name":"Chromatography (Basel)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45969641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2