Chromatography (Basel)最新文献_第5页

Nα-(5-Fluoro-2,4-dinitrophenyl)-L-leucinamide-derivatized LC/MS/MS Analysis of Amino Acid Enantiomers in HepG2 Cells n - α-(5-氟-2,4-二硝基苯)- l -亮氨酸衍生LC/MS/MS分析HepG2细胞氨基酸对映体

Chromatography (Basel) Pub Date : 2021-10-10 DOI: 10.15583/jpchrom.2021.014

Yusuke Takano, M. Takahashi, Masaki Kobayashi, Takeshi Uemura, T. Furuchi

引用次数: 1

Evaluation of the Photodegradation of Crushed- and Suspended Pranoprofen Tablets 碾碎和悬浮Pranoprofen片的光降解评价

Chromatography (Basel) Pub Date : 2021-10-07 DOI: 10.15583/jpchrom.2021.015

Kohei Kawabata, Mai Ishida, Shiori Akimoto, M. Inagaki, H. Nishi

引用次数: 3

Development of Core-Shell Ion-Exchange Resin by Changing the Core-Shell Ratio and Its Elution Behavior with Carbohydrates 改变核壳比制备核壳离子交换树脂及其与碳水化合物的洗脱行为

Chromatography (Basel) Pub Date : 2021-10-07 DOI: 10.15583/jpchrom.2021.013

S. Mitomo, Y. Negishi, T. Mutai, Y. Inoue

引用次数: 3

The Utilization of Triacontyl-Bonded Silica Coated with Imidazolium Ions for Capillary Ion Chromatographic Determination of Inorganic Anions 咪唑离子包被三硫醇键合二氧化硅毛细管离子色谱法测定无机阴离子

Chromatography (Basel) Pub Date : 2021-08-24 DOI: 10.15583/jpchrom.2021.009

Anang Sedyohutomo, Hiroshi Suzuki, C. Fujimoto

引用次数: 1

Multi-Functional Nanocavities Fabricated Using Molecular Imprinting and Post-Imprinting Modifications for Efficient Biomarker Detection 利用分子印迹和印迹后修饰制备多功能纳米腔用于高效生物标记物检测

Chromatography (Basel) Pub Date : 2021-05-31 DOI: 10.15583/JPCHROM.2021.011

Hirobumi Sunayama, T. Takeuchi

{"title":"Multi-Functional Nanocavities Fabricated Using Molecular Imprinting and Post-Imprinting Modifications for Efficient Biomarker Detection","authors":"Hirobumi Sunayama, T. Takeuchi","doi":"10.15583/JPCHROM.2021.011","DOIUrl":"https://doi.org/10.15583/JPCHROM.2021.011","url":null,"abstract":"Antibodies and enzymes are currently considered the gold-standard molecular recognition elements as they facilitate the construction of biosensing systems and exhibit high specificity and affinity toward target molecules. However, the low stability of such systems and high associated production cost limit the practical applications of antibodies and enzymes, thereby necessitating the development of alternative molecular recognition elements. Molecularly imprinted polymers (MIPs) are synthetic polymer receptors that are capable of molecular recognition. These polymers contain binding cavities of various shapes and sizes that are complementary to the target molecule and aid in the capture of target molecules. However, although the original procedure for generating MIPs, developed before 2000, is simple, the resulting binding activity and selectivity are inferior to those of antibodies. Meanwhile, post-imprinting modification (PIM) involves site-directed chemical modification of functional monomer residues within the molecularly imprinted cavities to alter MIP functionality. In this review, we provide an overview of sophisticated PIM techniques for developing highly sensitive MIPs that can be used to recognize biomarker proteins. Toward this, we draw heavily on information from our own recent work. This article has the potential to provide important insights that would aid the development of synthetic polymer materials for biosensing.","PeriodicalId":91226,"journal":{"name":"Chromatography (Basel)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45441589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Quantitative Determination of Gaseous Alkanes Adsorbed on Filter Paper During PM2.5 Sampling PM2.5取样过程中滤纸吸附气态烷烃的定量测定

Chromatography (Basel) Pub Date : 2021-05-09 DOI: 10.15583/JPCHROM.2021.010

I. Ueta, T. Koyama, Katsunori Sumiya, Yoshihiro Saito

引用次数: 1

LVSEP Analysis of Cationic Analytes in Cationic Polymer-Coating Microchannel Prepared by Vacuum-Drying Method 真空干燥法制备阳离子聚合物涂层微通道中阳离子分析物的LVSEP分析

Chromatography (Basel) Pub Date : 2021-04-21 DOI: 10.15583/JPCHROM.2021.008

F. Kitagawa, Yuka Niimiya, Isoshi Nukatsuka

引用次数: 1

Chromatographic Performance of an Amine/amino-bonded Column and a Monolithic Reversed-Phase Column for the Separation of Fluorescently Labeled Glycoprotein Glycans 胺/氨基键合柱和整体反相柱分离荧光标记糖蛋白聚糖的色谱性能

Chromatography (Basel) Pub Date : 2021-04-08 DOI: 10.15583/JPCHROM.2021.005

Maho Hioki, H. Kobayashi, M. Kinoshita, Sachio Yamamoto, Shigeo Suzuki

{"title":"Chromatographic Performance of an Amine/amino-bonded Column and a Monolithic Reversed-Phase Column for the Separation of Fluorescently Labeled Glycoprotein Glycans","authors":"Maho Hioki, H. Kobayashi, M. Kinoshita, Sachio Yamamoto, Shigeo Suzuki","doi":"10.15583/JPCHROM.2021.005","DOIUrl":"https://doi.org/10.15583/JPCHROM.2021.005","url":null,"abstract":"For the HPLC analysis of glycoprotein glycans, oligosaccharides released from glycoproteins are often derivatized with fluorescent tags to achieve quantitative separation. However, the influence of such fluorescent tags on the structure and retention of glycans is not completely understood. Here, to investigate the separation of labeled glycans, we prepared four types of derivatives (2-aminopyridine, 2-aminobenzoic acid, 2-aminobenzamide, and ethyl 4-aminobenzoate) of three model glycans (high-mannose oligosaccharides from ribonuclease B, complex oligosaccharides from bovine fetuin, and an isomaltooligosaccharide mixture). The glycan derivatives were separated on newly developed stationary phases, namely, an aminoand amide-bonded hydrophilic (AAH) phase and a capillary-packed monolithic ODS phase without end-capping treatment. The performance of these phases was compared with typical amide hydrophilic interaction chromatography (HILIC) and end-capped C18 packed columns. In the HILIC separation mode, the AAH column exhibited superior resolution for isomaltooligosaccharides, compared to those obtained on a conventional amide column. Nevertheless, the plate numbers were the same for glycoprotein glycans and the three linkage isomers of Man7GlcNAc2 could not be resolved, whereas they could be separated on the amide column. However, the cationic amino groups of the AAH column enhanced the resolution of sialylated complex glycans that could not be separated on the amide HILIC column. In the reversed-phase separation mode, the retention of glycan derivatives was mainly dependent on the hydrophobicity of the labeling groups. In contrast to the conventional packed ODS column, the monolithic capillary ODS column showed good resolution for neutral glycans.","PeriodicalId":91226,"journal":{"name":"Chromatography (Basel)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43869484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Molecular Shape Selectivity for Polycyclic Aromatic Compoundson a Poly(4-vinylpyridine) Stationary Phase in Liquid Chromatography 多环芳香族化合物在聚(4-乙烯基吡啶)固定相中的分子形状选择性

Chromatography (Basel) Pub Date : 2021-03-25 DOI: 10.15583/JPCHROM.2021.007

Koki Nakagami, K. Shimizu, Ohjiro Sumiya, I. Ueta, Yoshihiro Saito

{"title":"Molecular Shape Selectivity for Polycyclic Aromatic Compoundson a Poly(4-vinylpyridine) Stationary Phase in Liquid Chromatography","authors":"Koki Nakagami, K. Shimizu, Ohjiro Sumiya, I. Ueta, Yoshihiro Saito","doi":"10.15583/JPCHROM.2021.007","DOIUrl":"https://doi.org/10.15583/JPCHROM.2021.007","url":null,"abstract":"Poly(4-vinylpyridine) (P4VP) was introduced as a stationary phase in liquid chromatography (LC) and the retention behavior of polycyclic aromatic compounds (PACs) on the P4VP phase has been investigated by comparing with that observed on commercially-available octadecylsilica (ODS) and phenylbutylsilica (PBS) phases. On the P4VP phase, a good linear relationship between the logarithmic retention factor and the molecular size of planar PACs was obtained, however, a unique selectivity for some planar PACs was also observed. The P4VP phase demonstrated a specific retention trend, where planar PACs having \"square-like\" molecular shape were strongly retained. The above trend clearly suggested a unique selectivity of the P4VP phase for PACs. In addition, compared to the typical ODS and PBS phases, the P4VP phase showed a good molecular shape recognition capability for planar/non-planar PACs with a similar two-dimensional molecular size. The P4VP phase showed a very small retention for various alkylbenzenes, suggesting somewhat limited contribution of the hydrophobicity of the analytes to the retention. In the investigation for the selectivity to structural isomers, the P4VP phase exhibited a unique selectivity for isomers of dichlorobenzene and dibromobenzene when compared to the typical ODS and PBS phases. The trend can be interpreted as a dipole-dipole interaction between pyridyl groups in the P4VP ligands and the analyte.","PeriodicalId":91226,"journal":{"name":"Chromatography (Basel)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45458649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous Quantitative Screening for Pain Medications in Serum by High-Performance Liquid Chromatography/Time-of-Flight Mass Spectrometry with Solid-Phase Dispersive Extraction 高效液相色谱/飞行时间质谱-固相分散萃取同时定量筛选血清中止痛药

Chromatography (Basel) Pub Date : 2021-02-21 DOI: 10.15583/JPCHROM.2021.001

Koichi Saito, Rika Nishiyama, R. Ito

{"title":"Simultaneous Quantitative Screening for Pain Medications in Serum by High-Performance Liquid Chromatography/Time-of-Flight Mass Spectrometry with Solid-Phase Dispersive Extraction","authors":"Koichi Saito, Rika Nishiyama, R. Ito","doi":"10.15583/JPCHROM.2021.001","DOIUrl":"https://doi.org/10.15583/JPCHROM.2021.001","url":null,"abstract":"In this study, solid-phase dispersive extraction (SPDE) was used for serum pretreatment and in the simultaneous analysis of analgesics and adjuvant analgesics (30 types in total) that are usually used as first-and second-choice treatments for pain patients, by liquid chromatography/time-of-flight mass spectrometry (LC/TOF-MS). Examination of the optimum conditions for SPDE using Oasis MCX as the solid-phase gel revealed that the recovery rates for serum samples deproteinized in advance were 49–87%, whereas the recovery rates were as high as 78–112% when deproteinization was not performed. The matrix effect was within ±10% regardless of the presence or absence of deproteinization, and its influence could be suppressed even if deproteinization was not performed. The results indicate that serum deproteinization was unnecessary when SPDE was used for pretreatment. In LC/TOF-MS measurement, gradient elution was carried out using core-shell type column Kinetex C18 (150 mm × 2.1 mm, 1.7 µm) as the LC column and 50 mM ammonium acetate buffer (pH 7.8)/acetonitrile/methanol mixture as the mobile phase. The 30 drugs were well separated, and the limit of quantification was 0.25–10 ng/mL, the correlation coefficients of the calibration curves were higher than 0.998, and the average recoveries ranged from 77.7 to 112.1%. The method would be useful to screen for analgesics and adjuvant analgesics (30 types in total) in serum in the fields of forensic science and emergency medicine.","PeriodicalId":91226,"journal":{"name":"Chromatography (Basel)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44786832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0