Effect of Organic Modifier on the Retention of Low-Molecular-Weight Organic Compounds in Low-Temperature HPLC Using a Liquid CO2 Mobile Phase and an Octadecyl Stationary Phase

K. Matsuo, Mina Otsubo, Tomohiro Motono, S. Kitagawa, Yoshinori Iiguni, H. Ohtani
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引用次数: 1

Abstract

The retention behaviors of various low-molecular-weight compounds in low-temperature HPLC with an ODS (octadecyl silica) column and liquid CO 2 mobile phase containing methanol or acetonitrile as modifiers were investigated. The addition of methanol/acetonitrile reduced the retention of xanthone, which was dominated by the liquid CO 2 content, independent of the modifier. Additionally, the retention of polycyclic aromatic hydrocarbons (PAHs) was reduced by the addition of methanol/acetonitrile. Because acetonitrile resulted in a rapid reduction in the retention compared with methanol, it was suggested that the CH-  interactions have an important role in the retention of PAHs in the C18 stationary phase. Notably, the retention of alkylbenzenes were only slightly affected by the addition of methanol/acetonitrile. Acetonitrile slightly increased the retention of alkylbenzenes with longer alkyl chains than those with shorter alkyl chains. It is suggested that the interaction of the phenyl moiety in alkylbenzenes with C18 chains was reduced by the increase in the acetonitrile content, and enhanced in the case of the alkyl chain moieties. The retentions of alkyl benzenes were approximately compensated, when the alkyl chain length was relatively
采用液态CO2流动相和十八烷基固定相低温高效液相色谱研究有机改性剂对低分子量有机化合物保留的影响
采用ODS(十八烷基二氧化硅)柱,以甲醇或乙腈为改性剂的液态CO2流动相,研究了各种低分子量化合物在低温高效液相色谱中的保留行为。甲醇/乙腈的加入降低了黄酮的保留率,该保留率主要由液体CO2含量决定,与改性剂无关。此外,添加甲醇/乙腈可以降低多环芳烃(PAHs)的保留率。由于与甲醇相比,乙腈导致保留率迅速降低,因此认为CH- 相互作用在PAHs在C18固定相中的保留中具有重要作用。值得注意的是,添加甲醇/乙腈仅对烷基苯的保留有轻微影响。与烷基链较短的烷基苯相比,乙腈略微提高了烷基链较长的烷基苯的保留率。研究表明,烷基苯中苯基部分与C18链的相互作用随着乙腈含量的增加而减少,而在烷基链部分的情况下增强。当烷基链长度相对较小时,烷基苯的滞留得到了大致补偿
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