Matilda Ayim-Akonor, Doreen Dela Owusu-Ntumy, Hilda Emefa Ohene-Asa, Agyekum Oduro-Abrokwa, Patricia Hammond, Michael Appenteng, Daniel Annan
{"title":"Serological and Molecular Surveillance of Infectious Bronchitis Virus Infection in Free-Range Chickens and Guinea Fowls in the Ga-East District of Ghana.","authors":"Matilda Ayim-Akonor, Doreen Dela Owusu-Ntumy, Hilda Emefa Ohene-Asa, Agyekum Oduro-Abrokwa, Patricia Hammond, Michael Appenteng, Daniel Annan","doi":"10.1155/2018/4949580","DOIUrl":"https://doi.org/10.1155/2018/4949580","url":null,"abstract":"<p><p>Infectious bronchitis is an economically important disease with worldwide distribution. Information is available on the presence of infectious bronchitis virus in commercial chicken in parts of Ghana but there is no information on free-range poultry and guinea fowls in the country. Possible IBV infections among free-range chickens and guinea fowls in Abokobi and Frafraha communities in the Ga-East district of the Greater Accra Region of Ghana were investigated using serology and PCR. Blood, tracheal, and cloacal swabs were obtained from 219 free-range chickens and guinea fowls with no respiratory symptoms and no history of IBV vaccination. Sera were evaluated for IBV antibodies by ELISA using commercial IBV test kit from IDEXX, Inc., USA. Swab samples were evaluated for S1 glycoprotein gene by one-step RT PCR. All the swab samples tested negative for IBV. 16% of all tested sera were positive for IBV. IBV seroprevalence in guinea fowls was 0%. 21.2% of sera from local chickens were positive for IBV. The seroprevalence of IBV among local chickens from Frafraha was 30% and that of Abokobi was 7.7%. This study shows exposure of local chickens in the study communities to IBV.</p>","PeriodicalId":91135,"journal":{"name":"Journal of veterinary medicine","volume":"2018 ","pages":"4949580"},"PeriodicalIF":0.0,"publicationDate":"2018-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/4949580","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36442805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A Long-Term Study of a Lipid-Buprenorphine Implant in Rats.","authors":"Michael Guarnieri, Cory Brayton, Betty M Tyler","doi":"10.1155/2018/2616152","DOIUrl":"10.1155/2018/2616152","url":null,"abstract":"<p><p>Animal models to study opiates are of growing interest. We have examined the short-term safety of buprenorphine implants in Fischer F344/NTac rats treated with excess doses of a cholesterol-triglyceride suspension of buprenorphine. A single injection of 0.65 mg/kg afforded clinically significant blood levels of analgesia for 3 days. Chemistry, hematology, coagulation, and urinalysis values with 2- to 10-fold excess doses of the drug-lipid suspension were within normal limits. Histopathology findings were unremarkable. The skin and underlying tissue surrounding the drug injection were unremarkable. Here we report the results of a long-term follow-up study of female rats injected with 0.65 and 1.3 mg/kg. The 14-month evaluation showed no abnormal findings that could be attributed to the drug or lipid suspension. These results confirm the safety of cholesterol-triglyceride carrier systems for subcutaneous drug delivery in laboratory animals and suggest that this model may be used to study long-term effects of opiate therapy.</p>","PeriodicalId":91135,"journal":{"name":"Journal of veterinary medicine","volume":"2018 ","pages":"2616152"},"PeriodicalIF":0.0,"publicationDate":"2018-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/2616152","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36401666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparative Seroepidemiological Study of Brucellosis in Sheep under Smallholder Farming and Governmental Breeding Ranches of Central and North East Ethiopia.","authors":"Shimeles Abegaz Addis, Andualem Yimer Desalegn","doi":"10.1155/2018/7239156","DOIUrl":"https://doi.org/10.1155/2018/7239156","url":null,"abstract":"<p><p>This cross-sectional study was carried out to investigate the status of brucellosis in sheep management under extensive smallholder farming and intensively in governmental breeding ranches in six districts selected from three administrative zones. Using multistage sampling, serum samples of 2409 sheep from 274 flocks were collected and tested using the Rose Bengal Plate Agglutination Test (RBPT) and positive sera were confirmed using a Complement Fixation Test (CFT). Of all animals tested, 4.98% were RBPT positive, and after confirmation with CFT, the overall animal-level prevalence was found to be 4.89% (CI: 3.24-6.9%). Of the flocks sampled, 61 (22.3%, CI: 18.03-29.17%) had at least one animal positive to both tests. Significantly higher (<i>P</i> < 0.001) individual animal seroprevalence of 5.87% (CI: 3.83-7.31%) was found in sheep under smallholder production than in breeding ranches (1.75%, 95% CI: 1.57-3.05%). However, flock level seroprevalence in breeding ranches was found to be 100% (8/8), while in the smallholder production it was 19.92% (CI: 16.4-25.81%). Significantly highest animal-level seroprevalence of 9.55% (CI: 7.91-12.4%) was observed in north Wollo zone's smallholder farms. From the three studied breeding ranches, highest seroprevalence of 3.57% (CI: 2.84%-5.18%) was found in Sheno Agricultural Research Centre. Significantly higher seroprevalence (<i>P</i> < 0.01) was found in aborted sheep and with history of retained fetal membrane in both production systems. All the sheep flocks in the studied breeding ranches were found to be seropositive; hence, this study suggests strict control measures of ovine brucellosis in the breeding reaches, since they could be a source of infection for the smallholder farms.</p>","PeriodicalId":91135,"journal":{"name":"Journal of veterinary medicine","volume":"2018 ","pages":"7239156"},"PeriodicalIF":0.0,"publicationDate":"2018-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/7239156","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36368564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tshilenge M Georges, Masumu Justin, Mbao Victor, Kayembe Jean Marie, Rweyemamu Mark, Mulumba Mfumu K Léopold
{"title":"Seroprevalence and Virus Activity of Rift Valley Fever in Cattle in Eastern Region of Democratic Republic of the Congo.","authors":"Tshilenge M Georges, Masumu Justin, Mbao Victor, Kayembe Jean Marie, Rweyemamu Mark, Mulumba Mfumu K Léopold","doi":"10.1155/2018/4956378","DOIUrl":"https://doi.org/10.1155/2018/4956378","url":null,"abstract":"<p><p>Rift Valley fever (RVF) is a zoonotic disease that is characterized by periodic and severe outbreaks in humans and animals. Published information on the occurrence of RVF in domestic animals is very scarce in the Democratic Republic of the Congo (DRC). To assess possible circulation of Rift Valley fever virus (RVFV) in cattle in the eastern province of DRC, 450 sera collected from cattle in North Kivu, South Kivu, and Ituri provinces were analyzed using the enzyme-linked immunosorbent assay (ELISA), for the detection of viral Immunoglobulin (Ig) G and M, and reverse transcriptase polymerase chain reaction (RT-PCR), for detection of viral RVF RNA. A cumulative anti-RVF IgG prevalence of 6.22% (95% CI 4.25-8.97) was recorded from the three provinces sampled. In North Kivu and Ituri provinces the anti-RVF IgG prevalence was 12.67% [95% CI 7.80-19.07] and 6% [95% CI 2.78-11.08], respectively, while all the sera collected from South Kivu province were negative for anti-RVF IgG antibodies. Anti-RVF IgM prevalence of 1.8% was obtained among sampled animals in the three provinces. None of the positive anti-RVF IgM samples (n=8) was positive for viral RVFV RNA using RT-PCR. Our findings suggest that RVFV is widely distributed among cattle in eastern province of DRC particularly in North Kivu and Ituri provinces although the epidemiological factors supporting this virus circulation remain unknown in these areas.</p>","PeriodicalId":91135,"journal":{"name":"Journal of veterinary medicine","volume":"2018 ","pages":"4956378"},"PeriodicalIF":0.0,"publicationDate":"2018-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/4956378","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36347419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xiaobu Ye, MariaLisa Itzoe, Rachel Sarabia-Estrada, Louis DeTolla, Betty M Tyler, Michael Guarnieri
{"title":"Suspected Lonely Mouse Syndrome as a Cage Effect in a Drug Safety Study.","authors":"Xiaobu Ye, MariaLisa Itzoe, Rachel Sarabia-Estrada, Louis DeTolla, Betty M Tyler, Michael Guarnieri","doi":"10.1155/2018/9562803","DOIUrl":"https://doi.org/10.1155/2018/9562803","url":null,"abstract":"<p><p>Studies have demonstrated that buprenorphine, a front line drug for veterinary analgesia, may alleviate symptoms of chronic pain. A cage side observation protocol was used to record behavioral signs in a mouse clinical trial of extended release buprenorphine. A retrospective review of the observations for signs of pain and stress revealed that mice given a fivefold overdose of buprenorphine (16.25 mg/kg) showed lethargy and facial signs associated with stress. However, similar signs were observed in the drug-free control mice as early as Day 3 of single-cage housing. This appears to be the first report of cage effects in a clinical trial for a veterinary drug.</p>","PeriodicalId":91135,"journal":{"name":"Journal of veterinary medicine","volume":"2018 ","pages":"9562803"},"PeriodicalIF":0.0,"publicationDate":"2018-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/9562803","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36183079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Occurrences of Indigestible Foreign Bodies in Cattle Slaughtered at Morogoro Municipal Slaughterhouse, Tanzania.","authors":"S F Bwatota, M Makungu, H E Nonga","doi":"10.1155/2018/4818203","DOIUrl":"https://doi.org/10.1155/2018/4818203","url":null,"abstract":"<p><p>A cross-sectional study was conducted to determine the occurrence of indigestible foreign bodies (IFB) in cattle slaughtered at Morogoro Municipal Slaughterhouse, Tanzania. A total of 387 slaughter cattle were examined for presence of IFB. Out of 387 examined cattle, 93 (24.03%) had IFB in their forestomachs. The observed IFB were plastic bags, fruit seeds, clothing materials, ropes, hairballs, leather materials, stones, metallic nails, and wire. Plastic bags were the most frequently (50.5%) observed IFB followed by fruit seeds (18.3%). A significantly (<i>p</i> < 0.05) high proportion of old animals (31.7%) had IFB compared to the young animals (21.2%). Similarly, the frequency of occurrence of IFB was significantly high (<i>p</i> < 0.05) in crossbred dairy cattle (42.3%) compared to local breeds (22.7%). Cattle that appeared with poor body condition (37.8%) were found to be more affected (<i>p</i> < 0.05) by IFB than those with good body condition (15.9%). In 91.4% of animals which had IFB, all the materials were located in the rumen. This study showed that presence of IFB is a common problem in cattle slaughtered at Morogoro Municipal Slaughterhouse and may significantly cause poor production and mortality in affected animals. Therefore, appropriate solid waste disposal should be implemented.</p>","PeriodicalId":91135,"journal":{"name":"Journal of veterinary medicine","volume":"2018 ","pages":"4818203"},"PeriodicalIF":0.0,"publicationDate":"2018-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/4818203","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36031739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Isabelle F Vanhaezebrouck, Elizabeth A Ballegeer, Stephen Frey, Rob Sieffert
{"title":"Accuracy and Precision of a Veterinary Neuronavigation System for Radiation Oncology Positioning.","authors":"Isabelle F Vanhaezebrouck, Elizabeth A Ballegeer, Stephen Frey, Rob Sieffert","doi":"10.1155/2018/6431749","DOIUrl":"https://doi.org/10.1155/2018/6431749","url":null,"abstract":"<p><p>Conformal radiation treatment plans such as IMRT and other radiosurgery techniques require very precise patient positioning, typically within a millimeter of error for best results. CT cone beam, real-time navigation, and infrared position sensors are potential options for success but rarely present in veterinary radiation centers. A neuronavigation system (Brainsight Vet, Rogue Research) was tested 22 times on a skull for positioning accuracy and precision analysis. The first 6 manipulations allowed the authors to become familiar with the system but were still included in the analyses. Overall, the targeting mean error in 3D was 1.437 mm with SD 1.242 mm. This system could be used for positioning for radiation therapy or radiosurgery.</p>","PeriodicalId":91135,"journal":{"name":"Journal of veterinary medicine","volume":"2018 ","pages":"6431749"},"PeriodicalIF":0.0,"publicationDate":"2018-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2018/6431749","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36019562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Probiotics for the Control of Helminth Zoonosis.","authors":"Abadi Amare Reda","doi":"10.1155/2018/4178986","DOIUrl":"10.1155/2018/4178986","url":null,"abstract":"<p><p>This paper is a comprehensive, concise, and an up to date review about probiotics effect and mechanisms against helminth infections of zoonotic importance. Zoonoses are diseases that can be transmitted from animals to humans in a reversible way. Despite zoonotic helminth diseases being still a challenge to the public health and the agriculture industries globally, they were still neglected in both human and veterinary medicine. Moreover, the increasing emergence of anthelmintic drug resistance constitutes failures of most disease control strategies, alarming for a quest to new alternative control approaches. Consequently, the use of beneficial microorganisms, probiotics, is becoming interesting for its prophylactic or therapeutic application against several diseases including helminths. Recent studies on probiotics against parasites and the interactions between bacteria, parasites, and the immune system in the gut draw much attention. However, the effects of these beneficial microorganisms in helminth infections remain largely unexplored. Therefore, the aim of the present review is to raise attention and to summarize recent findings on probiotics research against helminth parasites of zoonotic significance. State-of-the-art research on beneficial effects of bacteria on helminth infections and their proposed mechanisms of action is thoroughly discussed.</p>","PeriodicalId":91135,"journal":{"name":"Journal of veterinary medicine","volume":"2018 ","pages":"4178986"},"PeriodicalIF":0.0,"publicationDate":"2018-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5831688/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36019561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Vaginoscopy in Ewes Utilizing a Laparoscopic Surgical Port Device.","authors":"Jeremiah Easley, Desiree Shasa, Eileen Hackett","doi":"10.1155/2017/7404371","DOIUrl":"https://doi.org/10.1155/2017/7404371","url":null,"abstract":"<p><p>Vaginoscopy allows for diagnostic evaluation and treatment of the vaginal vault. A laparoscopic surgical port device and rigid telescope were utilized for serial vaginoscopy in 8 healthy anesthetized ewes. Vaginoscopy examinations were performed in each ewe at days 1, 14, and 28. This technique was well-tolerated and facilitated carbon dioxide vaginal inflation, complete vaginal examination, identification of the cervix, and targeted biopsy collection. No complications were encountered during or following the vaginoscopy procedures. The laparoscopic port device was well-suited to the ewe vulvar size. This technique could be applied to clinical evaluation in ewes for the purposes of examination, biopsy, culture, foreign body removal, and minor surgical procedures.</p>","PeriodicalId":91135,"journal":{"name":"Journal of veterinary medicine","volume":"2017 ","pages":"7404371"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2017/7404371","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35251695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cloacael Carriage and Multidrug Resistance <i>Escherichia coli</i> O157:H7 from Poultry Farms, Eastern Ethiopia.","authors":"Mude Shecho, Naod Thomas, Jelalu Kemal, Yimer Muktar","doi":"10.1155/2017/8264583","DOIUrl":"https://doi.org/10.1155/2017/8264583","url":null,"abstract":"<p><p>A cross-sectional study was carried out to determine antimicrobial drug resistance patterns of <i>E. coli</i> O157:H7 isolates and estimate the level of the pathogen. A total of 194 cloacae swab samples were collected randomly in two poultry farms. Standard cultural, biochemical, and serological (latex agglutination) methods were used to isolate <i>E. coli</i> O157:H7. The isolates were subjected to antimicrobial susceptibility testing using disc diffusion method. Out of 194 cloacae samples examined, 13.4% (<i>n</i> = 26) were found to be positive for <i>E. coli</i> O157:H7. The finding indicated differences in <i>E. coli</i> O157:H7 infection among the different risk factors. Chicken from Adele Poultry Farm showed higher <i>E. coli</i> O157:H7 infection (OR = 3.89) than Haramaya University poultry farm and young birds had more infection (OR = 4.62) than adult birds. Of the total 14 antimicrobials included in the panel of study, the susceptibility results were varied with 96.15% and 0% <i>E. coli</i> O157:H7 isolates expressing resistance to erythromycin, clindamycin, spectinomycin, and ciprofloxacin, respectively. Multidrug resistance to more than two antimicrobial agents was detected in 24 (92.30%) of the isolates. The study showed high presence of antimicrobial resistant isolates of <i>E. coli</i> O157:H7. Further study is required to better understand the ecology and evolution of bacterial resistance to antimicrobial agents.</p>","PeriodicalId":91135,"journal":{"name":"Journal of veterinary medicine","volume":"2017 ","pages":"8264583"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2017/8264583","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34860885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}