{"title":"A Limited Sampling, Simple, and Useful Method for Determination of Glomerular Filtration Rate in Cats by Using a New Accurate HPLC Method to Measure Iohexol Plasmatic Concentrations.","authors":"Meucci Valentina, Guidi Grazia, Melanie Pierre, Breghi Gloria, Lippi Ilaria","doi":"10.1155/2013/569121","DOIUrl":"10.1155/2013/569121","url":null,"abstract":"<p><p>Glomerular filtration rate (GFR) is still a highly underutilized tool in cats because available methods are not easy to be performed in clinical practice. Iohexol (IOX) has been shown to be a useful and reliable marker of GFR both in animals and in humans. The aim of the present study was to develop a rapid and reliable method for measuring IOX in feline plasma and to evaluate the accuracy of limited sampling models to establish a low-cost and clinically suitable GFR test. IOX concentrations were determined by using a new HPLC-UV method. GFR was assessed as plasma clearance of IOX, which was calculated by dividing dose administered by area under the curve of plasmatic concentration versus time (AUC), and indexed to body weight (BW). Correlation and agreement analysis between the GFR values obtained by a seven-point clearance method and the GFR values determined by the application of simplified sample combinations indicated that the 3-blood sample clearance model (5, 30, and 60 min) was the best simplified method because it provided an accurate GFR value in only one hour. The reported method is a simple and accurate way of GFR determination, which may be easily used in a clinical setting. </p>","PeriodicalId":91135,"journal":{"name":"Journal of veterinary medicine","volume":"2013 ","pages":"569121"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4590869/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34086600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Carolina Guadalupe Sosa-Gutierrez, Maria Teresa Quintero Martinez, Soila Maribel Gaxiola Camacho, Silvia Cota Guajardo, Maria D Esteve-Gassent, María-Guadalupe Gordillo-Pérez
{"title":"Frequency and Clinical Epidemiology of Canine Monocytic Ehrlichiosis in Dogs Infested with Ticks from Sinaloa, Mexico.","authors":"Carolina Guadalupe Sosa-Gutierrez, Maria Teresa Quintero Martinez, Soila Maribel Gaxiola Camacho, Silvia Cota Guajardo, Maria D Esteve-Gassent, María-Guadalupe Gordillo-Pérez","doi":"10.1155/2013/797019","DOIUrl":"https://doi.org/10.1155/2013/797019","url":null,"abstract":"<p><p>Ehrlichia canis is a rickettsial intracellular obligate bacterial pathogen and agent of canine monocytic ehrlichiosis. The prevalence of this disease in veterinary medicine can vary depending on the diagnostic method used and the geographic location. One hundred and fifty-two canine blood samples from six veterinary clinics and two shelters from Sinaloa State (Mexico) were analyzed in this study. All animals were suspected of having Canine Monocytic Ehrlichiosis (CME). The diagnostic methods used were the ELISA (Snap4Dx, IDEXX) together with blood smear and platelet count. From all dogs blood samples analyzed, 74.3% were positive to E. canis by ELISA and 40.1% were positive by blood smear. The sensitivity and specificity observed in the ELISA test were 78.8% and 86.7%. In addition, thrombocytopenia was presented in 87.6% of positive dogs. The predominant clinical manifestations observed were fever, anorexia, depression, lethargy, and petechiae. Consequently, this is the first report in which the morulae were visualized in the blood samples, and E. canis-specific antibodies were detected in dogs from Sinaloa, Northwest of Mexico. </p>","PeriodicalId":91135,"journal":{"name":"Journal of veterinary medicine","volume":"2013 ","pages":"797019"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/797019","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34152668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ibrahim Abdullah Ibrahim, Ali Mahmoud Shamat, Mohammed Osman Hussien, Abdel Rahim Mohammed El Hussein
{"title":"Profile of Some Trace Elements in the Liver of Camels, Sheep, and Goats in the Sudan.","authors":"Ibrahim Abdullah Ibrahim, Ali Mahmoud Shamat, Mohammed Osman Hussien, Abdel Rahim Mohammed El Hussein","doi":"10.1155/2013/736497","DOIUrl":"https://doi.org/10.1155/2013/736497","url":null,"abstract":"<p><p>One hundred camels (Camelus dromedaries) and fifty sheep and goats being adult, male, and apparently healthy field animals were studied to provide data regarding the normal values of some hepatic trace elements. Liver samples were collected during postmortem examination, digested, and analyzed for Cu, Zn, Fe, Co, and Mn using atomic absorption spectrophotometry. The results showed that the differences in mean liver concentrations of Cu, Zn, Fe, and Co between camels, sheep, and goats were statistically significant (P < 0.05). Hepatic Cu, Fe, and Co concentrations were higher in camels than in sheep and goats. All liver samples were adequate for Fe and Co, whereas only camel liver was adequate for Cu. In camels, hepatic Zn concentration was inadequately lower than that in sheep and goats. No difference in Mn concentration was detected between camels, sheep, and goats. All liver samples were inadequate compared to free-ranging herbivores. In camels, significant correlation (r (2) = -0.207, P value = 0.04) was detected between Zn and Co, whereas in sheep significant correlation (r (2) = -0.444, P value = 0.026) was detected between Zn and Mn. No significant correlation between trace elements was detected in goats. </p>","PeriodicalId":91135,"journal":{"name":"Journal of veterinary medicine","volume":"2013 ","pages":"736497"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/736497","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34152667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Eva Tvrdá, Norbert Lukáč, Monika Schneidgenová, Jana Lukáčová, Csaba Szabó, Zofia Goc, Agnieszka Greń, Peter Massányi
{"title":"Impact of Seminal Chemical Elements on the Oxidative Balance in Bovine Seminal Plasma and Spermatozoa.","authors":"Eva Tvrdá, Norbert Lukáč, Monika Schneidgenová, Jana Lukáčová, Csaba Szabó, Zofia Goc, Agnieszka Greń, Peter Massányi","doi":"10.1155/2013/125096","DOIUrl":"https://doi.org/10.1155/2013/125096","url":null,"abstract":"<p><p>Mutual relationships between selected chemical elements (Na, K, Fe, Cu, Mg, and Zn), basic motility characteristics (motility and progressive motility), and markers of the oxidative balance (superoxide dismutase, catalase, glutathione, albumin, and malondialdehyde) were investigated in bovine seminal plasma and spermatozoa. Computer assisted sperm analysis was used to assess the motility parameters; mineral concentrations were determined by the voltammetric method and flame absorption spectrophotometry; antioxidants and malondialdehyde were evaluated by UV/VIS spectrophotometry. Concentrations of chemical elements in both seminal fractions were in the following descending order: Na > K > Zn > Mg > Fe > Cu. Higher amounts of all minerals and nonenzymatic antioxidants were detected in the seminal plasma (P < 0.01; P < 0.001), while higher MDA concentration and activity of enzymatic antioxidants were recorded in the cell lysates (P < 0.01; P < 0.001). Na, Fe, Cu, Mg, and Zn were positively correlated with the motility and antioxidant parameters (P < 0.05; P < 0.01; P < 0.001). Inversely, K exhibited the positive associations with malondialdehyde (P < 0.05). This study demonstrates that most chemical elements are integral components of bovine semen and are needed for the protection against oxidative stress development. </p>","PeriodicalId":91135,"journal":{"name":"Journal of veterinary medicine","volume":"2013 ","pages":"125096"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/125096","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34086594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ayne Murata Hayashi, Ana Carolina Brandão Campos Fonseca Pinto, Silvia Renata Gaido Cortopassi, Valdecir Marvulle, Jessica Ruivo Maximino, Gerson Chadi, Julia Maria Matera
{"title":"S100β Levels in CSF of Nonambulatory Dogs with Intervertebral Disk Disease Treated with Electroacupuncture.","authors":"Ayne Murata Hayashi, Ana Carolina Brandão Campos Fonseca Pinto, Silvia Renata Gaido Cortopassi, Valdecir Marvulle, Jessica Ruivo Maximino, Gerson Chadi, Julia Maria Matera","doi":"10.1155/2013/549058","DOIUrl":"https://doi.org/10.1155/2013/549058","url":null,"abstract":"<p><p>The aim of the study was to investigate S100β levels in the cerebrospinal fluid of nonambulatory dogs with intervertebral disk disease treated with electroacupuncture: 10 dogs with thoracolumbar disk extrusion graded 3 to 5 (EA group) and 7 dogs without neurologic dysfunction (control group). All dogs regained ambulation. S100β was detected by Western blot analysis where EA group dogs were evaluated at two time points (M1 = before EA and M2 = when the dogs return ambulation) and at one time point from control group. In EA group dogs M1-S100β levels were significantly higher than in control group. EA group dogs were divided into subgroups A (n = 7-early motor recovery; 6.7 ± 7.8 days) and B (n = 3-late motor recovery; 76 ± 17.0 days). M1-S100β levels were similar between subgroups A and B. However, M2-S100β levels were significantly higher in subgroup B than in subgroup A. An elevated S100β levels were observed in dogs with late motor recovery. S100β may be associated with neuroplasticity following spinal cord injuries with intervertebral disk extrusion. Further studies with larger numbers of subjects and control group with affected dogs are necessary to investigate the relationship between neurotrophic factors and electroacupuncture stimulation. </p>","PeriodicalId":91135,"journal":{"name":"Journal of veterinary medicine","volume":"2013 ","pages":"549058"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/549058","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34086599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Traumatic Reticuloperitonitis in Water Buffalo (Bubalus bubalis): Clinical Findings and the Associated Inflammatory Response.","authors":"Maged El-Ashker, Mohamed Salama, Mohamed El-Boshy","doi":"10.1155/2013/808656","DOIUrl":"https://doi.org/10.1155/2013/808656","url":null,"abstract":"<p><p>The present study was carried out to describe the clinical picture of traumatic reticuloperitonitis (TRP) in water buffalo (Bubalus bubalis) and to evaluate the inflammatory and immunologic responses for this clinical condition. Twenty-two buffalo with acute local TRP were monitored in our study. Additionally, 10 clinically healthy buffalo were randomly selected and served as controls. Acute local TRP was initially diagnosed by clinical examination and confirmed by ultrasonographic (USG) examination and/or necropsy findings. Blood samples were collected from all examined buffalo to measure the respective levels of tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, IL-10 and interferon gamma (INF)-γ, serum amyloid A (SAA), C-reactive protein (CRP), haptoglobin (Hp), fibrinogen (Fb), and serum sialic acid (SSA). It was found that TNF-α, IL-1β, IL-6, IL-10, SAA, CRP, Hp, Fb, and SSA were significantly higher in buffalo with TRP than the controls. Our findings suggest that the examined immunologic variables were helpful in documenting the inflammatory response in buffalo with TRP. However, their diagnostic usefulness only becomes apparent when considered in tandem with the clinical findings for any given animal, its anamnesis, and a subsequent USG assessment. Due to the frequent complications of TRP, more accurate indicators of its occurrence and severity would be useful. </p>","PeriodicalId":91135,"journal":{"name":"Journal of veterinary medicine","volume":"2013 ","pages":"808656"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/808656","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34152669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Surgical Management of Penile and Preputial Neoplasms in Equine with Special Reference to Partial Phallectomy.","authors":"Awad Rizk, Esam Mosbah, Gamal Karrouf, Mohamed Abou Alsoud","doi":"10.1155/2013/891413","DOIUrl":"https://doi.org/10.1155/2013/891413","url":null,"abstract":"<p><p>Penile and preputial neoplasia in horses occurs infrequently and represents diagnostic and therapeutic challenges. The present study was carried out on a total number of 21 equids (14 stallions and 7 donkeys) suffered from different penile and preputial neoplasia. Diagnosis of neoplasms was based up on history of the case, clinical examination as well as histopathological evaluation. Animals with penile and preputial neoplasms were underwent local excision and partial phallectomy with a slightly modified version of the techniques described by William's. The diagnosed neoplasms were penile and preputial squamous cell carcinomas (SCCs; n = 15); sarcoid (n = 4); a-fibrosarcoma; and a melanoma. Local excision was curative in all cases except 5 stallions with SCCs. These stallions had extensive damage of the glans penis, free part of the penis and the inner lamina of the internal fold of the prepuce, and they underwent a partial phallectomy with successful outcome. Follow-up information was obtained by visit and telephone inquiries. In conclusion, penile and preputial neoplasms are commonly encountered in elderly male horses and SCCs are the most common type affecting male external genitalia. Partial phallectomy is effective for management of equine neoplasia if they are confined to the glans and body of the penis and there is no proximal spread or involvement to regional lymph nodes. </p>","PeriodicalId":91135,"journal":{"name":"Journal of veterinary medicine","volume":"2013 ","pages":"891413"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/891413","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34152673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Khalda A Khalifa, Egbal Sidahmed Abdelrahim, Magdi Badwi, Amal M Mohamed
{"title":"Isolation and Molecular Characterization of Mycoplasma gallisepticum and Mycoplasma synoviae in Chickens in Sudan.","authors":"Khalda A Khalifa, Egbal Sidahmed Abdelrahim, Magdi Badwi, Amal M Mohamed","doi":"10.1155/2013/208026","DOIUrl":"https://doi.org/10.1155/2013/208026","url":null,"abstract":"<p><p>The current study described the isolation and molecular detection of Mycoplasma gallisepticum (Mg) and Mycoplasma synoviae from tracheal swabs of diseased birds showing signs of respiratory distress in selected commercial (layer and broiler) farms and from yolk and an open air of pens of vaccinated breeder flocks in Sudan. A number of 45 Mycoplasma isolates were recovered from chickens in Khartoum, Gezira, and Equatoria states in Sudan. Of these, eight Mg and three Ms isolates were identified using growth inhibition and rapid serum agglutination (RSA) tests. The conventional PCR technique was applied to amplify 140 bp and 720 bp DNA fragments for the Mg and Ms, respectively. This research confirmed vertical and horizontal transmission of Mg from breeder farms through detection of Mg in yolk of fertile eggs and an air of pens despite previous vaccination. PCR is considered a rapid, sensitive, and cheap method and it will improve the diagnosis of Mycoplasma in chickens. </p>","PeriodicalId":91135,"journal":{"name":"Journal of veterinary medicine","volume":"2013 ","pages":"208026"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/208026","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34086595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}