Bio-medical materials and engineering最新文献

{"title":"Assessment of decellularization strategy and biocompatibility testing of full-thickness abdominal wall to produce a tissue-engineered graft.","authors":"George Skepastianos, Panagiotis Mallis, Epameinondas Kostopoulos, Efstathios Michalopoulos, Vasileios Skepastianos, Christos Doudakmanis, Chrysoula Palazi, Gerasimos Tsourouflis","doi":"10.3233/BME-240144","DOIUrl":"10.3233/BME-240144","url":null,"abstract":"<p><strong>Background: </strong>Restoration of the abdominal wall defects due to herniation or other complications represents a challenging task of the reconstructive surgery. Synthetic grafts or crosslinked animal-derived grafts, are utilized, followed by significant adverse reactions.</p><p><strong>Objective: </strong>This study aimed to evaluate primarily the production of a decellularized abdominal wall scaffold and secondly its biocompatibility upon transplantation in an animal model.</p><p><strong>Methods: </strong>Full-thickness abdominal wall samples were harvested from Wistar Rats and then decellularized utilizing a three-cycle process. To evaluate the decellularization efficacy, histological, biochemical and biomechanical analyses were performed. The biocompatibility assessment involved the implantation of the produced scaffolds to Sprague Dawley rats. The grafts remained for a total period of 4 weeks, followed by immunohistochemistry for the detection of CD11b+, CD4+ and CD8+ cells.</p><p><strong>Results: </strong>Histological, biochemical and biomechanical results, indicated the production of compatible acellular full-thickness abdominal wall samples. After 4 weeks of implantation, a minor presence of immunity cells was observed.</p><p><strong>Conclusion: </strong>The data of this study indicated the successful production of a full-thickness abdominal wall scaffold. Biologically derived full-thickness abdominal wall scaffolds may have greater potential in restoration of the abdominal wall defects, bringing them one step closer to their clinical utility.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"43-53"},"PeriodicalIF":1.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142341567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of bioceramic inclusions on gel-cast aliphatic polymer membranes for bone tissue engineering applications: An <i>in vitro</i> study.","authors":"Vasudev Vivekanand Nayak, Edmara T P Bergamo, Vijayavenkataraman Sanjairaj, Rakesh Kumar Behera, Nikhil Gupta, Paulo G Coelho, Lukasz Witek","doi":"10.3233/BME-240079","DOIUrl":"10.3233/BME-240079","url":null,"abstract":"<p><strong>Background: </strong>Polylactic acid (PLA) has been extensively used in tissue engineering. However, poor mechanical properties and low cell affinity have limited its pertinence in load bearing bone tissue regeneration (BTR) devices.</p><p><strong>Objective: </strong>Augmenting PLA with β-Tricalcium Phosphate (β-TCP), a calcium phosphate-based ceramic, could potentially improve its mechanical properties and enhance its osteogenic potential.</p><p><strong>Methods: </strong>Gels of PLA and β-TCP were prepared of different % w/w ratios through polymer dissolution in acetone, after which polymer-ceramic membranes were synthesized using the gel casting workflow and subjected to characterization.</p><p><strong>Results: </strong>Gel-cast polymer-ceramic constructs were associated with significantly higher osteogenic capacity and calcium deposition in differentiated osteoblasts compared to pure polymer counterparts. Immunocytochemistry revealed cell spreading over the gel-cast membrane surfaces, characterized by trapezoidal morphology, distinct rounded nuclei, and well-aligned actin filaments. However, groups with higher ceramic loading expressed significantly higher levels of osteogenic markers relative to pure PLA membranes. Rule of mixtures and finite element models indicated an increase in theoretical mechanical strength with an increase in β-TCP concentration.</p><p><strong>Conclusion: </strong>This study potentiates the use of PLA/β-TCP composites in load bearing BTR applications and the ability to be used as customized patient-specific shape memory membranes in guided bone regeneration.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"15-33"},"PeriodicalIF":1.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142341568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosynthesis of gold nanoparticles and their protective effect towards diabetic nephropathy by inhibition of oxidative stress.","authors":"Bo Qu, Yuhua He, Shanlin Liu, Yulian Huang, Yingchun Bai","doi":"10.1177/09592989241296432","DOIUrl":"10.1177/09592989241296432","url":null,"abstract":"<p><strong>Background: </strong>Diabetes is an emerging health issue on a global scale, with increasing prevalence rates reported in many countries. Many mechanisms are proposed for diabetic nephropathy, with oxidative stress being the most significant. The effectiveness of gold nanoparticles (AuNPs) in the attenuation of nephropathy and oxidative stress in diabetic mice was assessed in this study.</p><p><strong>Objective: </strong>The aim of this study is to synthesize AuNPs and assess their protective effect towards diabetic nephropathy by inhibition of oxidative stress.</p><p><strong>Methods: </strong>The aqueous extract of <i>Allium sativum</i> was employed to synthesize AuNPs. The prepared AuNPs were characterized using a variety of microscopic and spectroscopic techniques. <i>In-vitro</i> studies were conducted using mice. Streptozotocin (STZ) was employed to induce diabetes in rodents. After 7 days of administration of STZ, anesthesia was given to all animals and blood was collected for the assessment of creatinine and Blood Urea Nitrogen (BUN) levels. Later, kidney tissue was removed at 4 °C and changes in pathology and oxidative stress were assessed.</p><p><strong>Results: </strong>Nephropathy was confirmed in diabetic mice by the changes in the pathology of kidney tissue along with significant rise in the plasma levels of BUN and creatinine. Additionally, the peroxidation of lipids, formation of Reactive Oxygen Species (ROS), oxidation of glutathione (GSH), concentration of carbonyl protein was also increased in the tissue of kidney of diabetic mice. Oxidative stress in kidney tissue and changes in the pathology of diabetic mice were inhibited significantly (p < 0.05) with the treatment of AuNPs.</p><p><strong>Conclusion: </strong>This study revealed the protective effects of AuNPs over diabetic nephropathy by inhibiting the pathway of oxidative stress. Since, the prepared AuNPs showed improvement over complications of diabetes, they may be believed as a potential gratuitous treatment next to other drugs for reducing blood glucose.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":"36 1","pages":"54-66"},"PeriodicalIF":1.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143413415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and performance analysis of zinc-iron-based nanomaterials for targeted transport.","authors":"Wenwen Cheng, Jin Zhang, Na Lin, Ding Yuan, Shuai Zhang, Zhonggang Yang, Tianyi Cao","doi":"10.1177/09592989241296437","DOIUrl":"10.1177/09592989241296437","url":null,"abstract":"<p><strong>Background: </strong>Nanomaterials have applications in traditional Chinese medicine in the fields of medical equipment manufacturing, targeted transportation, and drug synergistic therapy.</p><p><strong>Objective: </strong>The research aims to discuss the performance and performance of zinc-iron-based nanomaterials in medical drug delivery and synergistic drug therapy.</p><p><strong>Methods: </strong>Using Prussian materials as precursors, magnetic zinc-iron nanomaterials were prepared by ZnCl₂ and K₃[Fe (CN)₆]. Moreover, the morphology and composition of the material were analyzed.</p><p><strong>Results: </strong>X-ray analysis was conducted on the prepared Zn₃[Fe (CN)₆]₂·xH₂O nanomaterials, and their purity met the design requirements. At the same time, drug loading analysis was conducted on Zn₃[Fe(CN)₆]₂·xH₂O, and the release of capsaicin reached 86.3% under a certain phosphate buffer solution. Meanwhile, Zn₃[Fe (CN)₆]₂·xH2O loaded tetracycline could release up to 90% in phosphate buffer solution. Antibacterial tests were conducted on self-made Zn₃[Fe(CN)₆]₂·xH₂O samples and ZnFe₂O₄/ZnO. The Zn₃[Fe(CN)₆]₂·xH₂O samples showed a more significant inhibitory effect on cancer cells after loading with capsaicin.</p><p><strong>Conclusion: </strong>The zinc-iron-based nanomaterials prepared by the research have excellent performance in drug loading and safety, indicating their significant potential for development in the medical field.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":"36 1","pages":"3-14"},"PeriodicalIF":1.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143413416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temperature dependence of the protective effect of pressurized dissolution of xenon gas during cold storage of cells.","authors":"Kenshi Mimura, Rina Sakai, Kazuhiro Yoshida, Masanobu Ujihira","doi":"10.3233/BME-240105","DOIUrl":"10.3233/BME-240105","url":null,"abstract":"<p><strong>Background: </strong>The preservation period afforded by cold storage of cells is short. However, the use of rare gases for cold storage as a means of extending the period of preservation would be highly beneficial.</p><p><strong>Objective: </strong>To examine the effect of temperature on the protective effect of cold storage of cells using pressurized dissolution of xenon gas, with particular focus on the inhibition of substance transport by viscosity.</p><p><strong>Methods: </strong>Human dermal fibroblast monolayers incubated in a culture dish for 48 h were used as a test sample, with culture medium used as a preservation solution. Samples were placed into a pressure-resistant vessel, which was pressurized with xenon gas at 0 or 0.5 MPa, and cells were stored at 0 to 5°C for 18 h. Cell activity was evaluated by tetrazolium salt assay. The viscosity of the medium under pressurization at each storage temperature was estimated.</p><p><strong>Results: </strong>The maximum protective effect against cell damage of cold storage with pressurized dissolution of xenon gas was observed at 4°C. An increase in estimated viscosity by pressurization was correlated with increased cell activity at 4°C.</p><p><strong>Conclusion: </strong>Analysis of the temperature dependence of the protective effect against cell damage of cold storage with pressurized dissolution of xenon gas revealed that the most effective temperature is 4°C. The data also suggest that increased viscosity due to pressurization plays a role in the protective effect.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"530-535"},"PeriodicalIF":1.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142139303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extract from <i>Falcaria vulgaris</i> loaded with exosomes for the treatment of hypertension in pregnant mice: <i>In vitro</i> and <i>In vivo</i> investigations.","authors":"Jing Chen, Huan Wang, Jing Zhu","doi":"10.3233/BME-240053","DOIUrl":"10.3233/BME-240053","url":null,"abstract":"<p><strong>Background: </strong>Hypertensive disorders during pregnancy pose significant risks to both maternal and fetal health, necessitating safe and effective therapeutic interventions.</p><p><strong>Objective: </strong>This study aimed to investigate the potential of an extract derived from <i>Falcaria vulgaris</i> (FV), loaded with exosomes to form the Exo/FV complex, as a novel therapeutic agent for the management of hypertension in pregnant mice: antioxidants, antimicrobials, and phenolic compounds present in FV lower blood pressure.</p><p><strong>Methods: </strong>The isolation of exosomes was done by ultracentrifugation methods and the FV was loaded into the exosomes by electroporation method.</p><p><strong>Results: </strong>The Exo/FV was found to be spherical with diameter ranges from 20 to 30 nm and they were tested for biocompatibility in NHI 3T3 cell lines and found to be effective. This research investigated <i>in vivo</i> hypertension in mice induced by L-NAME and treated with FV and Exo/FV and found that AChE and MAO determine mice's redox state tends to reduce blood pressure. Increased non-protein thiol (NP-SH) and decreased lipid peroxidation were also found, and PDE-5, ACE, Arginase, and MDA activity has also been tested.</p><p><strong>Conclusion: </strong>This analysis showed that Exo/FV effectively treated hypertension during pregnancy.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"509-521"},"PeriodicalIF":1.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142370960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SIKVAV promotion proliferation of vascular endothelial cells and related mechanisms.","authors":"Xionglin Chen, Jie Zhang, Xiaoming Cao, He Jiang, Zhiren Wu, Zi du Zeng, Chen Jiang, Hui Chen","doi":"10.3233/BME-240018","DOIUrl":"10.3233/BME-240018","url":null,"abstract":"<p><strong>Background: </strong>Vascular endothelial injury, a key factor in diabetic foot ulcers (DFUs) pathogenesis, is linked to the impaired proliferation and migration of vascular endothelial cells, modulated by hypoxia-inducible factor, growth factors, and inflammatory elements.</p><p><strong>Objective: </strong>The present study assesses the role of SIKVAV (Ser-Ile-Lys-Val-Ala-Val), a peptide shown to enhance cell proliferation and migration, on mouse aortic endothelial cell (MAEC) and the corresponding molecular mechanisms.</p><p><strong>Methods: </strong>MAEC were treated with SIKVAV at 0, 100, 200, 400, and 600 μg/mL for 0, 24, 48, and 72 h. Cell viability was tested using the CCK-8 assay. Proliferating cell nuclear antigen (PCNA), extracellular signal-regulated kinase 1/2 (ERK1/2), and protein kinase B (Akt) levels were measured by qRT-PCR and western blot.</p><p><strong>Results: </strong>SIKVAV augmented PCNA mRNA expression and stimulated vascular endothelial cell proliferation in a concentration and time-dependent fashion. Furthermore, it amplified the expression of p-ERK1/2 and p-Akt, pivotal components of the mitogen-activated protein kinase (MAPK)/ERK1/2 and phosphatidylinositol-3-kinase (PI3K)/Akt signaling pathways. The inhibition of these pathways suppressed PCNA mRNA expression, cell proliferation rate, and decreased p-ERK1/2 and p-Akt levels, highlighting SIKVAV's role in promoting vascular endothelial cell proliferation via these pathways.</p><p><strong>Conclusion: </strong>The results of this study confirmed that SIKVAV grafted onto scaffolds can accelerate the proliferation of vascular endothelial cells for the therapy of skin wounds, and provide a theoretical basis for its application in ischemic disease as synthesized biomaterials scaffolds of tissue engineering.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"499-508"},"PeriodicalIF":1.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142139302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accuracy of an image matching technique for assessing knee alignment during the stance phase of gait using single-plane anteroposterior radiography.","authors":"Koichi Kobayashi, Makoto Sakamoto, Tatsuya Soeno, Takashi Sato","doi":"10.3233/BME-240059","DOIUrl":"10.3233/BME-240059","url":null,"abstract":"<p><strong>Background: </strong>Accurate measurement of bone alignment of the knee during walking provides ideal clinical information for diagnosis and treatment of knee joint disorders. To bring this ideal closer to clinical reality, we developed an image matching technique to measure the three-dimensional (3D) position of bones using anteroposterior radiography during the stance phase of treadmill walking.</p><p><strong>Objective: </strong>To develop and validate an image matching method for evaluation of 3D knee alignment using anteroposterior radiography of artificial femoral and tibial bones.</p><p><strong>Methods: </strong>The 3D position of each bone was recovered by minimizing the difference between the projected outline and the contour of the bone in the anteroposterior radiograph. The true value of the position was measured using a 3D coordinate measuring machine.</p><p><strong>Results: </strong>The mean values ± standard deviation and root mean squares (RMS) of translation errors were within -1.6 ± 2.1 mm and 2.6 mm, respectively, for femur, and 2.1 ± 1.9 mm and 2.8 mm for tibia. The mean values ± standard deviation and RMS of errors in rotation were within 0.3 ± 0.7° and 0.7°, respectively, for femur, and -0.3 ± 0.9° and 0.9°, respectively, for tibia.</p><p><strong>Conclusion: </strong>Our method is suitable for evaluating 3D knee alignment on anteroposterior radiography.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"522-529"},"PeriodicalIF":1.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142035217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acupuncture navigation method integrated with augmented reality.","authors":"Shin-Yan Chiou, Meng-Ru He","doi":"10.3233/BME-240073","DOIUrl":"10.3233/BME-240073","url":null,"abstract":"<p><strong>Background: </strong>Acupuncture and moxibustion are effective in alleviating symptoms, but the large number of acupoints can make accurate needle placement and training difficult.</p><p><strong>Objective: </strong>To address these challenges, this study aims to develop an augmented reality (AR) acupuncture navigation system designed to improve the accuracy and intuitiveness of acupoint localization.</p><p><strong>Methods: </strong>The proposed system employs a six-point registration and positioning technique, enabling the AR navigation model to adapt to the specific characteristics of each patient.</p><p><strong>Results: </strong>In testing, discrepancies between virtual and actual acupuncture points ranged from 0.6 mm to 3.9 mm, which is within the acceptable tolerance range for acupuncture.</p><p><strong>Conclusion: </strong>This AR-based system shows promise in enhancing the precision of acupuncture point localization, potentially leading to improved treatment outcomes.</p>","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"536-547"},"PeriodicalIF":1.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142341566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Testing the shock protection performance of Type I construction helmets using impactors of different masses","authors":"John Z. Wu, Christopher S. Pan, Mahmood Ronaghi, Bryan M. Wimer","doi":"10.3233/bme-230173","DOIUrl":"https://doi.org/10.3233/bme-230173","url":null,"abstract":"BACKGROUND:Wearing protective helmets is an important prevention strategy to reduce work-related traumatic brain injuries. The existing standardized testing systems are used for quality control and do not provide a quantitative measure of the helmet performance. OBJECTIVE:To analyze the failure characterizations of Type I industrial helmets and develop a generalized approach to quantify the shock absorption performance of Type I industrial helmets based on the existing standardized setups. METHODS:A representative basic Type I construction helmet model was selected for the study. Top impact tests were performed on the helmets at different drop heights using two different impactor masses (3.6 and 5.0 kg). RESULTS:When the helmets were impacted with potential impact energies smaller than the critical potential impact energy values, there was a consistent relationship between the peak impact force and the potential impact energy. When the helmets were impacted under potential impact energies greater than the critical potential impact energy values, the peak impact forces increased steeply with increasing potential impact energy. CONCLUSION:A concept of safety margin for construction helmets based on potential impact energy was introduced to quantify the helmets’ shock absorption performance. The proposed method will help helmet manufacturers improve their product quality.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":"11 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140799333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}