Bio-medical materials and engineering最新文献_第7页

Determining optimal length of coracoid graft in the modified Bristow procedure for anterior shoulder instability: A three-dimensional finite element analysis. 确定改良布里斯托手术中治疗肩关节前侧不稳定的最佳冠状带移植长度：三维有限元分析。

IF 1 4区医学

Bio-medical materials and engineering Pub Date : 2024-01-01 DOI: 10.3233/BME-230071

Hirotaka Sano, Tatsuro Komatsuda, Kazuhide Suzuki, Hiroo Abe, Hiroshi Ozawa, Jun Kumagai, Toshimitsu A Yokobori

{"title":"Determining optimal length of coracoid graft in the modified Bristow procedure for anterior shoulder instability: A three-dimensional finite element analysis.","authors":"Hirotaka Sano, Tatsuro Komatsuda, Kazuhide Suzuki, Hiroo Abe, Hiroshi Ozawa, Jun Kumagai, Toshimitsu A Yokobori","doi":"10.3233/BME-230071","DOIUrl":"10.3233/BME-230071","url":null,"abstract":"Background: There is a lack of consensus concerning the coracoid graft length in the modified Bristow procedure.Objective: We attempted to determine the optimal graft length using the three-dimensional finite element method.Methods: In a shoulder model with a 25% anterior glenoid defect, a coracoid graft of varying lengths (5, 10, 15, and 20 mm) was fixed using a half-threaded screw. First, a compressive load of 500 N was applied to the screw head to determine the graft failure load during screw tightening. Next, a tensile load (200 N) was applied to the graft to determine the failure load due to biceps muscle traction.Results: In the screw compression, the failure loads in the 5-, 10-, 15-, and 20-mm models were 252, 370, 377, and 331 N, respectively. In the tensile load applied to the coracoid graft, the failure load exceeded 200 N for both the 5- and 10-mm models.Conclusion: The 5-mm graft had a high risk of fracture during intraoperative screw tightening. As for the biceps muscle traction, the 5- and 10-mm-grafts had a lower failure risk than the 15- and 20-mm-grafts. Therefore, we believe that the optimal length of the coracoid graft is 10 mm in the modified Bristow procedure.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"65-75"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9761602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eichner classification based on panoramic X-ray images using deep learning: A pilot study. 利用深度学习对全景 X 光图像进行艾希纳分类：试点研究。

IF 1 4区医学

Bio-medical materials and engineering Pub Date : 2024-01-01 DOI: 10.3233/BME-230217

Yuta Otsuka, Hiroko Indo, Yusuke Kawashima, Tatsuro Tanaka, Hiroshi Kono, Masafumi Kikuchi

{"title":"Eichner classification based on panoramic X-ray images using deep learning: A pilot study.","authors":"Yuta Otsuka, Hiroko Indo, Yusuke Kawashima, Tatsuro Tanaka, Hiroshi Kono, Masafumi Kikuchi","doi":"10.3233/BME-230217","DOIUrl":"10.3233/BME-230217","url":null,"abstract":"Background: Research using panoramic X-ray images using deep learning has been progressing in recent years. There is a need to propose methods that can classify and predict from image information.Objective: In this study, Eichner classification was performed on image processing based on panoramic X-ray images. The Eichner classification was based on the remaining teeth, with the aim of making partial dentures. This classification was based on the condition that the occlusal position was supported by the remaining teeth in the upper and lower jaws.Methods: Classification models were constructed using two convolutional neural network methods: the sequential and VGG19 models. The accuracy was compared with the accuracy of Eichner classification using the sequential and VGG19 models.Results: Both accuracies were greater than 81%, and they had sufficient functions for the Eichner classification.Conclusion: We were able to build a highly accurate prediction model using deep learning scratch sequential model and VGG19. This predictive model will become part of the basic considerations for future AI research in dentistry.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"377-386"},"PeriodicalIF":1.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141287772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual therapy of cancer using optimal control supported by swarm intelligence 利用蜂群智能支持的优化控制对癌症进行双重治疗

IF 1 4区医学

Bio-medical materials and engineering Pub Date : 2023-12-28 DOI: 10.3233/bme-230150

Poh Ling Tan, Jeevan Kanesan, Joon Huang Chuah, Irfan Anjum Badruddin, Abdallah Abdellatif, Sarfaraz Kamangar, Mohamed Hussien, Maughal Ahmed Ali Baig, N. Ameer Ahammad

{"title":"Dual therapy of cancer using optimal control supported by swarm intelligence","authors":"Poh Ling Tan, Jeevan Kanesan, Joon Huang Chuah, Irfan Anjum Badruddin, Abdallah Abdellatif, Sarfaraz Kamangar, Mohamed Hussien, Maughal Ahmed Ali Baig, N. Ameer Ahammad","doi":"10.3233/bme-230150","DOIUrl":"https://doi.org/10.3233/bme-230150","url":null,"abstract":"BACKGROUND:The scientific revolution in the treatment of many illnesses has been significantly aided by stem cells. This paper presents an optimal control on a mathematical model of chemotherapy and stem cell therapy for cancer treatment. OBJECTIVE:To develop effective hybrid techniques that combine the optimal control theory (OCT) with the evolutionary algorithm and multi-objective swarm algorithm. The developed technique is aimed to reduce the number of cancerous cells while utilizing the minimum necessary chemotherapy medications and minimizing toxicity to protect patients’ health. METHODS:Two hybrid techniques are proposed in this paper. Both techniques combined OCT with the evolutionary algorithm and multi-objective swarm algorithm which included MOEA/D, MOPSO, SPEA II and PESA II. This study evaluates the performance of two hybrid techniques in terms of reducing cancer cells and drug concentrations, as well as computational time consumption. RESULTS:In both techniques, MOEA/D emerges as the most effective algorithm due to its superior capability in minimizing tumour size and cancer drug concentration. CONCLUSION:This study highlights the importance of integrating OCT and evolutionary algorithms as a robust approach for optimizing cancer chemotherapy treatment.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":"55 1","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139373421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing chemotherapy treatment outcomes using metaheuristic optimization algorithms: A case study 利用元启发式优化算法优化化疗疗效：案例研究

IF 1 4区医学

Bio-medical materials and engineering Pub Date : 2023-12-21 DOI: 10.3233/bme-230149

Prakas Gopal Samy, J. Kanesan, IrfanAnjum Badruddin, S. Kamangar, N. A. Ahammad

{"title":"Optimizing chemotherapy treatment outcomes using metaheuristic optimization algorithms: A case study","authors":"Prakas Gopal Samy, J. Kanesan, IrfanAnjum Badruddin, S. Kamangar, N. A. Ahammad","doi":"10.3233/bme-230149","DOIUrl":"https://doi.org/10.3233/bme-230149","url":null,"abstract":"BACKGROUND: This study explores the dynamics of a mathematical model, utilizing ordinary differential equations (ODE), to depict the interplay between cancer cells and effector cells under chemotherapy. The stability of the equilibrium points in the model is analysed using the Jacobian matrix and eigenvalues. Additionally, bifurcation analysis is conducted to determine the optimal values for the control parameters. OBJECTIVE: To evaluate the performance of the model and control strategies, benchmarking simulations are performed using the PlatEMO platform. METHODS: The Pure Multi-objective Optimal Control Problem (PMOCP) and the Hybrid Multi-objective Optimal Control Problem (HMOCP) are two different forms of optimal control problems that are solved using revolutionary metaheuristic optimisation algorithms. The utilization of the Hypervolume (HV) performance indicator allows for the comparison of various metaheuristic optimization algorithms in their efficacy for solving the PMOCP and HMOCP. RESULTS: Results indicate that the MOPSO algorithm excels in solving the HMOCP, with M-MOPSO outperforming for PMOCP in HV analysis. CONCLUSION: Despite not directly addressing immediate clinical concerns, these findings indicates that the stability shifts at critical thresholds may impact treatment efficacy.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":"45 22","pages":""},"PeriodicalIF":1.0,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138950139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomechanical effect of fibular osteotomy on the knee joint in high tibial osteotomy: A cadaveric study. 胫骨高位截骨中腓骨截骨对膝关节的生物力学影响:一项尸体研究。

IF 1 4区医学

Bio-medical materials and engineering Pub Date : 2023-01-01 DOI: 10.3233/BME-221395

Osamu Tanifuji, Tomoharu Mochizuki, Yoshio Koga, Yuji Tanabe, Hiroyuki Kawashima

{"title":"Biomechanical effect of fibular osteotomy on the knee joint in high tibial osteotomy: A cadaveric study.","authors":"Osamu Tanifuji, Tomoharu Mochizuki, Yoshio Koga, Yuji Tanabe, Hiroyuki Kawashima","doi":"10.3233/BME-221395","DOIUrl":"https://doi.org/10.3233/BME-221395","url":null,"abstract":"Background: Closed-wedge high tibial osteotomy (CWHTO) with a fibular osteotomy (FO) causes medial joint space widening in the knee. However, the effect of FO on the joint space width remains unclear.Objective: This study aimed to examine the effect of FO on the knee in HTO.Methods: A compression load test was performed on two amputated human limbs under four conditions: (1) normal (without any osteotomy), (2) open-wedge HTO (OWHTO), (3) OWHTO with FO, and (4) CWHTO. The contact area of the femoral and tibial cartilages and the medial and lateral joint space widths in each condition were evaluated using a motion capture system with computed tomography (CT) and magnetic resonance imaging (MRI) data.Results: The contact area increased on the lateral side after OWHTO, which increased more on the lateral side with a concomitant decrease on the medial side in both subjects when FO was added to OWHTO. An increase in the medial joint space width and a decrease on the lateral side were seen in both OWHTO with FO and CWHTO.Conclusions: The contact area and joint space widths are affected by the FO, and the effect is more pronounced than the way of HTO (OWHTO or CWHTO).","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":"34 2","pages":"123-131"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9148056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Reduced fibrous capsule elastic fibers from biologic ECM-enveloped CIEDs in minipigs, supported with a novel compression mechanics model. 在小型猪生物ecm包膜cied中减少纤维囊弹性纤维，支持新的压缩力学模型。

IF 1 4区医学

Bio-medical materials and engineering Pub Date : 2023-01-01 DOI: 10.3233/BME-221488

Roche C de Guzman, Allison S Meer, Aidan A Mathews, Atara R Israel, Michael T Moses, Clarence M Sams, Daniel B Deegan

{"title":"Reduced fibrous capsule elastic fibers from biologic ECM-enveloped CIEDs in minipigs, supported with a novel compression mechanics model.","authors":"Roche C de Guzman, Allison S Meer, Aidan A Mathews, Atara R Israel, Michael T Moses, Clarence M Sams, Daniel B Deegan","doi":"10.3233/BME-221488","DOIUrl":"https://doi.org/10.3233/BME-221488","url":null,"abstract":"Background: Fibrous capsules (Fb) in response to cardiovascular implantable electronic devices (CIEDs), including a pacemaker (P) system, can produce patient discomfort and difficulties in revision surgery due partially to their increased compressive strength, previously linked to elevated tissue fibers.Objective: A preliminary study to quantify structural proteins, determine if biologic extracellular matrix-enveloped CIEDs (PECM) caused differential Fb properties, and to implement a realistic mechanical model.Methods: Retrieved Fb (-P and -PECM) from minipigs were subjected to biomechanical (shear oscillation and uniaxial compression) and histological (collagen I and elastin) analyses.Results: Fb-PECM showed significant decreases compared to Fb-P in: low strain-loss modulus (390 vs. 541 Pa) across angular frequencies, high strain-compressive elastic modulus (1043 vs. 2042 kPa), and elastic fiber content (1.92 vs. 3.15 μg/mg tissue). Decreases in elastin were particularly noted closer to the implant's surface (Fb-PECM = 71% vs. Fb-P = 143% relative to dermal elastin at mid-tangential sections) and verified with a solid mechanics hyperelasticity with direction-dependent fiber viscoelasticity compression simulation (r2 ≥ 98.9%).Conclusions: The biologic envelope composed of decellularized porcine small intestine submucosa ECM for CIEDs promoted fibrous tissues with less elastic fibers. Novel compression modeling analyses directly correlated this singular reduction to more desirable subcutaneous tissue mechanics.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":"34 4","pages":"289-304"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10357198/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10224308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comparative study of robot-assisted and traditional surgeries in the treatment of thoracolumbar fractures based on 1-year follow-up observation. 基于1年随访观察的机器人辅助与传统手术治疗胸腰椎骨折的比较研究。

IF 1 4区医学

Bio-medical materials and engineering Pub Date : 2023-01-01 DOI: 10.3233/BME-222521

Xu-Qi Hu, Hui-Gen Lu, Xiao Zhou, Min-Jie Hu, Xue-Kang Pan, Bao Chen, Ye-Feng Yu

{"title":"A comparative study of robot-assisted and traditional surgeries in the treatment of thoracolumbar fractures based on 1-year follow-up observation.","authors":"Xu-Qi Hu, Hui-Gen Lu, Xiao Zhou, Min-Jie Hu, Xue-Kang Pan, Bao Chen, Ye-Feng Yu","doi":"10.3233/BME-222521","DOIUrl":"https://doi.org/10.3233/BME-222521","url":null,"abstract":"Background: There are conflicting results for robot-assisted (RA) pedicle screw fixation compared with freehand (FH) pedicle screw fixation.Objective: This study was designed to retrospectively compare the accuracy and efficacy of RA percutaneous pedicle screw fixation and traditional freehand FH pedicle screw fixation in the treatment of thoracolumbar fractures.Methods: A total of 26 cases were assigned to the RA group, and 24 cases were assigned to the FH group. The operation time, bleeding volume, and visual analog scale (VAS) score 1 day after the operation, and the anterior/posterior (A/P) vertebral height ratio of the injured vertebrae at 3 days and at internal fixation removal 1 year after the operation were compared between the two groups. Pedicle screw position accuracy was assessed according to Gertzbein criteria.Results: The operation times of the RA group and FH group were 138.69 ± 32.67 minutes and 103.67 ± 14.53 minutes, respectively, and the difference was statistically significant. The intraoperative blood loss was 49.23 ± 22.56 ml in the RA group and 78.33 ± 23.90 ml in the FH group, and the difference was statistically significant. There was a significant difference in the A/P vertebral height ratio of the injured vertebrae 3 days after the operation compared with before the operation in both groups (P < 0.05). There was a significant difference in the A/P vertebral height ratio of the injured vertebrae 3 days after the operation compared with that at fixation removal in both groups (P < 0.05).Conclusion: The application of RA orthopedic treatment for thoracolumbar fractures can achieve good fracture reduction.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":"34 4","pages":"375-383"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9780466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human adipose-derived stem cells can optimize the filling material in rats. 人脂肪源性干细胞可以优化大鼠的填充材料。

IF 1 4区医学

Bio-medical materials and engineering Pub Date : 2023-01-01 DOI: 10.3233/BME-222503

Siwei Qu, Ning Ma, Weixin Wang, Sen Chen, Qi Wu, Yangqun Li, Zhe Yang

{"title":"Human adipose-derived stem cells can optimize the filling material in rats.","authors":"Siwei Qu, Ning Ma, Weixin Wang, Sen Chen, Qi Wu, Yangqun Li, Zhe Yang","doi":"10.3233/BME-222503","DOIUrl":"https://doi.org/10.3233/BME-222503","url":null,"abstract":"Background: Human adipose-derived stem cells have been identified as a promising candidate for cell-assisted therapy to improve graft survival.Objective: To objective of the study was to add human adipose-derived stem cells into filling materials.Methods: The filling materials were prepared and divided into 6 groups: fat particles with phosphate buffer saline or human adipose-derived stem cells; acellular dermal matrix particles with phosphate buffer saline or human adipose-derived stem cells; mixture of fat particles and acellular dermal matrix particles with phosphate buffer saline or human adipose-derived stem cells. The survival rate, vascular density and histological at 2, 6 and 12 weeks were investigated.Results: Human adipose-derived stem cells significantly improved survival rate in each group at 6 and 12 weeks, and it significantly increased the vascular density in the fat particles and porcine acellular dermal matrix combined group and porcine acellular dermal matrix group at three time points, but human adipose-derived stem cells did not have a significant effect in the fat particles group.Conclusion: Human adipose-derived stem cells as assisted cells added into filling material can improve survival rate and vascular density in rats.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":"34 4","pages":"345-355"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9842621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design modifications and evaluation of the silicone artificial finger joints. 硅胶假指关节的设计修改与评价。

IF 1 4区医学

Bio-medical materials and engineering Pub Date : 2023-01-01 DOI: 10.3233/BME-222527

F A Alnaimat, H A Owida, Jamal I Al-Nabulsi, B Al-Naami, A Al-Ghraibah, M Al-Ayyad, Muneera Altayeb, Ashraf Al Sharah

{"title":"Design modifications and evaluation of the silicone artificial finger joints.","authors":"F A Alnaimat, H A Owida, Jamal I Al-Nabulsi, B Al-Naami, A Al-Ghraibah, M Al-Ayyad, Muneera Altayeb, Ashraf Al Sharah","doi":"10.3233/BME-222527","DOIUrl":"10.3233/BME-222527","url":null,"abstract":"Background: There are many reasons that could lead to finger joint arthroplasty, and the most familiar reason is osteoarthritis. Silicone finger joint are the most commonly used implants. However, these implants might fracture with time and cause wear which will lead to chronic inflammation and synovitis for the patient and then implant failure.Objective: The aim of this study is to improve the design of the silicone finger joint and simulate the different designs using finite element analysis (FEA) simulation.Method: Three different designs were drawn and FEA has been used in this study using Solidworks software. The first design is the silicone finger joint design without any modification, the second one is modified design with added ribs to the junction of distal stem and hinge and the third design was added filler material inside the body of the artificial joint. An axial force with 625 N that was applied on the upper part of the distal stem which is nearly represents the maximum value of the grip strength for normal males.Results: The results showed improvement on the design in which the concentrated stress at the junction of the distal stem and hinge of the design was distributed. In addition, the Von Mises stress was stable for the modified design with added ribs and the added filler material designs after 15°.Conclusion: The design modification could improve the stress distribution and stability of the artificial finger joint and increase the lifetime expectancy of these implants.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":" ","pages":"481-490"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10302835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental study on co-culture of DiI-labeled rat bone marrow mesenchymal stem cells and neonatal rat cardiomyocytes to induce differentiation into cardiomyocyte-like cells. dii标记大鼠骨髓间充质干细胞与新生大鼠心肌细胞共培养诱导分化为心肌细胞样细胞的实验研究。

IF 1 4区医学

Bio-medical materials and engineering Pub Date : 2023-01-01 DOI: 10.3233/BME-221429

Junsheng Mu, Zichang Zhang, Fan Zhou, Jianwei Zheng, Ping Bo, Bin You

{"title":"Experimental study on co-culture of DiI-labeled rat bone marrow mesenchymal stem cells and neonatal rat cardiomyocytes to induce differentiation into cardiomyocyte-like cells.","authors":"Junsheng Mu, Zichang Zhang, Fan Zhou, Jianwei Zheng, Ping Bo, Bin You","doi":"10.3233/BME-221429","DOIUrl":"https://doi.org/10.3233/BME-221429","url":null,"abstract":"Background: Myocardial infarction is a serious clinical disease with high mortality and poor prognosis. Cardiomyocytes (CMs) have limited regeneration abilities after ischemic injury. Their growth and differentiation can be enhanced by contact co-culture with stem cells.Objective: The aim was to study the contact co-culture of Dil-labeled bone marrow mesenchymal stem cells (BMSCs) and CMs for inducing differentiation of CMs from stem cells for treating myocardial infarction.Methods: After contact co-culture, the differentiation of BMSCs into CMs was analyzed qualitatively by detecting myocardial markers (cardiac troponin T and α-smooth muscle actin) using immunofluorescence and quantitatively using flow cytometry. To examine the mechanism, possible gap junctions between BMSCs and CMs were analyzed by detecting gap junction protein connexin 43 (C×43) expression in BMSCs using immunofluorescence. The functionality of gap junctions was analyzed using dye transfer experiments.Results: The results revealed that BMSCs in contact with CMs exhibited myocardial markers and a significant increase in differentiation rate (P < 0.05); they also proved the existence and function of gap junctions between BMSCs and CMs.Conclusions: It was shown that contact co-culture can induce Dil-labeled BMSCs to differentiate into CM-like cells and examined the principle of gap junction-mediated signaling pathways involved in inducing stem cells to differentiate into cardiomyocytes.","PeriodicalId":9109,"journal":{"name":"Bio-medical materials and engineering","volume":"34 4","pages":"319-330"},"PeriodicalIF":1.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9841594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0