Biophysical reviews最新文献_第8页

Primitive membraneless compartments as a window into the earliest cells. 原始的无膜隔室是进入最早细胞的窗口

IF 4.9

Biophysical reviews Pub Date : 2023-09-08 eCollection Date: 2023-12-01 DOI: 10.1007/s12551-023-01135-9

Tony Z Jia

引用次数: 0

Tools for microbial single-cell genomics for obtaining uncultured microbial genomes. 用于获得未培养微生物基因组的微生物单细胞基因组学工具

Biophysical reviews Pub Date : 2023-09-08 eCollection Date: 2024-02-01 DOI: 10.1007/s12551-023-01124-y

Masahito Hosokawa, Yohei Nishikawa

{"title":"Tools for microbial single-cell genomics for obtaining uncultured microbial genomes.","authors":"Masahito Hosokawa, Yohei Nishikawa","doi":"10.1007/s12551-023-01124-y","DOIUrl":"10.1007/s12551-023-01124-y","url":null,"abstract":"The advent of next-generation sequencing technologies has facilitated the acquisition of large amounts of DNA sequence data at a relatively low cost, leading to numerous breakthroughs in decoding microbial genomes. Among the various genome sequencing activities, metagenomic analysis, which entails the direct analysis of uncultured microbial DNA, has had a profound impact on microbiome research and has emerged as an indispensable technology in this field. Despite its valuable contributions, metagenomic analysis is a \"bulk analysis\" technique that analyzes samples containing a wide diversity of microbes, such as bacteria, yielding information that is averaged across the entire microbial population. In order to gain a deeper understanding of the heterogeneous nature of the microbial world, there is a growing need for single-cell analysis, similar to its use in human cell biology. With this paradigm shift in mind, comprehensive single-cell genomics technology has become a much-anticipated innovation that is now poised to revolutionize microbiome research. It has the potential to enable the discovery of differences at the strain level and to facilitate a more comprehensive examination of microbial ecosystems. In this review, we summarize the current state-of-the-art in microbial single-cell genomics, highlighting the potential impact of this technology on our understanding of the microbial world. The successful implementation of this technology is expected to have a profound impact in the field, leading to new discoveries and insights into the diversity and evolution of microbes.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10937852/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48393548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protein-DNA recognition mechanisms and specificity. 蛋白质- dna识别机制和特异性

IF 4.9

Biophysical reviews Pub Date : 2023-09-07 eCollection Date: 2023-10-01 DOI: 10.1007/s12551-023-01137-7

Anastasia A Anashkina

引用次数: 0

Water as a sensor of weak impacts on biological systems. 水作为对生物系统影响微弱的传感器

IF 4.9

Biophysical reviews Pub Date : 2023-09-07 eCollection Date: 2023-10-01 DOI: 10.1007/s12551-023-01120-2

Valentin I Lobyshev

{"title":"Water as a sensor of weak impacts on biological systems.","authors":"Valentin I Lobyshev","doi":"10.1007/s12551-023-01120-2","DOIUrl":"10.1007/s12551-023-01120-2","url":null,"abstract":"A characteristic feature of weak impacts is the non-monotonic response of living organisms and model biological systems to monotonically decreasing impacts. The qualitative similarity of the effects caused by the different acting factors makes one think about the common cause of the observed effects, which is water. A comprehensive analysis of the actual composition of water indicates that water under normal conditions is a multicomponent open non-equilibrium system. Nanobubbles that are always present in water play a significant role in the properties of dilute aqueous solutions. When collapsed, they can produce active oxygen and nitrogen species that have a strong effect on biological systems. Significant non-monotonic changes in electrical conductivity found in a series of sequentially diluted solutions subjected to vigorous shaking after each dilution convincingly demonstrate the presence of chemical changes in the composition of aqueous solutions explained by mechanochemical processes. Similar changes were observed in water samples prepared in the same manner with vigorous shaking and dilution without the addition of any chemical compounds. The long-term evolution of the conductivity of such solutions depends on the chemical structure of the solutes.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2023-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10643777/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42625949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Terahertz spectroscopy as a method for investigation of hydration shells of biomolecules. 太赫兹光谱作为研究生物分子水合壳的一种方法

IF 4.9

Biophysical reviews Pub Date : 2023-09-07 eCollection Date: 2023-10-01 DOI: 10.1007/s12551-023-01131-z

Nikita V Penkov

{"title":"Terahertz spectroscopy as a method for investigation of hydration shells of biomolecules.","authors":"Nikita V Penkov","doi":"10.1007/s12551-023-01131-z","DOIUrl":"10.1007/s12551-023-01131-z","url":null,"abstract":"The hydration of biomolecules is one of the fundamental processes underlying the construction of living matter. The formation of the native conformation of most biomolecules is possible only in an aqueous environment. At the same time, not only water affects the structure of biomolecules, but also biomolecules affect the structure of water, forming hydration shells. However, the study of the structure of biomolecules is given much more attention than their hydration shells. A real breakthrough in the study of hydration occurred with the development of the THz spectroscopy method, which showed that the hydration shell of biomolecules is not limited to 1-2 layers of strongly bound water, but also includes more distant areas of hydration with altered molecular dynamics. This review examines the fundamental features of the THz frequency range as a source of information about the structural and dynamic characteristics of water that change during hydration. The applied approaches to the study of hydration shells of biomolecules based on THz spectroscopy are described. The data on the hydration of biomolecules of all main types obtained from the beginning of the application of THz spectroscopy to the present are summarized. The emphasis is placed on the possible participation of extended hydration shells in the realization of the biological functions of biomolecules and at the same time on the insufficient knowledge of their structural and dynamic characteristics.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2023-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10643733/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47929712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversity and features of proteins with structural repeats. 具有结构重复的蛋白质的多样性及其特征

IF 4.9

Biophysical reviews Pub Date : 2023-09-07 eCollection Date: 2023-10-01 DOI: 10.1007/s12551-023-01130-0

Evgeniya I Deryusheva, Andrey V Machulin, Oxana V Galzitskaya

引用次数: 0

Quantitative measurement of influenza virus transmission in animal model: an overview of current state. 流感病毒在动物模型中传播的定量测量:现状概述

IF 4.9

Biophysical reviews Pub Date : 2023-09-07 eCollection Date: 2023-10-01 DOI: 10.1007/s12551-023-01113-1

Galina Onkhonova, Andrei Gudymo, Maksim Kosenko, Vasiliy Marchenko, Alexander Ryzhikov

{"title":"Quantitative measurement of influenza virus transmission in animal model: an overview of current state.","authors":"Galina Onkhonova, Andrei Gudymo, Maksim Kosenko, Vasiliy Marchenko, Alexander Ryzhikov","doi":"10.1007/s12551-023-01113-1","DOIUrl":"10.1007/s12551-023-01113-1","url":null,"abstract":"Influenza virus transmission is a crucial factor in understanding the spread of the virus within populations and developing effective control strategies. Studying the transmission patterns of influenza virus allows for better risk assessment and prediction of disease outbreaks. By monitoring the spread of the virus and identifying high-risk populations and geographic areas, it is possible to allocate resources more effectively, implement timely interventions, and provide targeted healthcare interventions to diminish the burden of influenza virus on vulnerable populations. Theoretical models of virus transmission are used to study and simulate of influenza virus spread within populations. These models aim to capture the complex dynamics of transmission, including factors such as population size, contact patterns, infectiousness, and susceptibility. Animal models serve as valuable tools for studying the dynamics of influenza virus transmission. This article presents a brief overview of existing research on the qualitative and quantitative study of influenza virus transmission in animal models. We discuss the methodologies employed, key insights gained from these studies, and their relevance.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2023-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10643727/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46101510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modeling transient ischemic attack via photothrombosis. 通过光血栓形成模拟短暂性脑缺血发作

IF 4.9

Biophysical reviews Pub Date : 2023-09-04 eCollection Date: 2023-10-01 DOI: 10.1007/s12551-023-01121-1

Y N Kalyuzhnaya, A M Khaitin, S V Demyanenko

{"title":"Modeling transient ischemic attack via photothrombosis.","authors":"Y N Kalyuzhnaya, A M Khaitin, S V Demyanenko","doi":"10.1007/s12551-023-01121-1","DOIUrl":"10.1007/s12551-023-01121-1","url":null,"abstract":"The health significance of transient ischemic attacks (TIAs) is largely underestimated. Often, TIAs are not given significant importance, and in vain, because TIAs are a predictor of the development of serious cardiovascular diseases and even death. Because of this, and because of the difficulty in diagnosing the disease, TIAs and related microinfarcts are poorly investigated. Photothrombotic models of stroke and TIA allow reproducing the occlusion of small brain vessels, even single ones. When dosing the concentration of photosensitizer, intensity and irradiation time, it is possible to achieve occlusion of well-defined small vessels with high reproducibility, and with the help of modern methods of blood flow assessment it is possible to achieve spontaneous restoration of blood flow without vessel rupture. In this review, we discuss the features of microinfarcts and the contemporary experimental approaches used to model TIA and microinfarcts, with an emphasis on models using the principle of photothrombosis of brain vessels. We review modern techniques for in vivo detection of blood flow in small brain vessels, as well as biomarkers of microinfarcts.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":null,"pages":null},"PeriodicalIF":4.9,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10643708/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41802343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Towards the synthesis of spectral imaging and machine learning-based approaches for non-invasive phenotyping of plants. 基于光谱成像和机器学习的植物非侵入性表型综合方法

IF 4.9

Biophysical reviews Pub Date : 2023-09-04 eCollection Date: 2023-10-01 DOI: 10.1007/s12551-023-01125-x

Alexei Solovchenko, Boris Shurygin, Dmitry A Nesterov, Dmitry V Sorokin

引用次数: 0

Biophysical Reviews (ISSUE 4 2023): LAFeBS-highlighting biophysics in Latin America. 生物物理评论》（第 4 2023 期）：LAFeBS--突出拉丁美洲的生物物理学。

IF 4.9

Biophysical reviews Pub Date : 2023-08-29 eCollection Date: 2023-08-01 DOI: 10.1007/s12551-023-01117-x

R Daniel Peluffo, Silvia Del V Alonso, Rosangela Itri, F Luis González Flecha, Leandro Barbosa

引用次数: 0