Biophysical reviews最新文献_第8页

Recent progress in probing small molecule interactions with DNA. 探测小分子与DNA相互作用的最新进展。

IF 5

Biophysical reviews Pub Date : 2025-11-27 eCollection Date: 2025-08-01 DOI: 10.1007/s12551-025-01373-z

Simon Poole, Bríonna McGorman, Christine J Cardin, Andrew Kellett

引用次数: 0

Recent advances in anticancer drug-membrane interactions: insights from Langmuir monolayers and molecular dynamics simulations. 抗癌药物-膜相互作用的最新进展：来自Langmuir单层和分子动力学模拟的见解。

IF 3.7

Biophysical reviews Pub Date : 2025-11-25 eCollection Date: 2026-02-01 DOI: 10.1007/s12551-025-01384-w

María Pedrosa, Arturo Moncho-Jordá, María José Gálvez-Ruiz

{"title":"Recent advances in anticancer drug-membrane interactions: insights from Langmuir monolayers and molecular dynamics simulations.","authors":"María Pedrosa, Arturo Moncho-Jordá, María José Gálvez-Ruiz","doi":"10.1007/s12551-025-01384-w","DOIUrl":"10.1007/s12551-025-01384-w","url":null,"abstract":"This review aims to provide an overview of recent studies on anticancer drug-cell membrane interactions using Langmuir monolayers (LMs) and molecular dynamics (MD) simulations. Cancer is a disease with high global incidence, and the prognosis remains concerning, with the World Health Organization predicting further increases in the coming years. However, substantial progress has been made in recent decades, particularly regarding the lipid composition of cell membranes. Cancer cell membranes are now known to have lipid profiles distinct from those of healthy cells, offering opportunities for new biomarkers and earlier diagnosis. Membranes serve as the first barrier that anticancer drugs must overcome, making their study vital to understanding drug mechanisms and resistance. Simplified models such as liposomes, micelles, and Langmuir monolayers have long been used to mimic biological membranes. Recent research has improved these models by incorporating the unique lipid compositions of cancerous and healthy cells, as well as by investigating novel anticancer agents, including drug nanocarriers. Advances in Langmuir monolayer techniques, coupled with complementary methods, allow for a deeper exploration of drug effects on membranes, enabling a distinction between their impacts on cancer and healthy cells. Computational tools, such as molecular dynamics simulations, further enrich our understanding by providing molecular-level insights into drug-membrane interactions. Integrating experimental and computational findings is essential for uncovering mechanisms of action and addressing drug resistance. These advances collectively contribute to the development of more effective anticancer therapies and to refining our understanding of the complex interplay between anticancer drugs and cell membranes.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"18 1","pages":"11-56"},"PeriodicalIF":3.7,"publicationDate":"2025-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13031497/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147572482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ian C. P. Smith and nucleic acids. Ian C. P. Smith和核酸。

IF 3.7

Biophysical reviews Pub Date : 2025-11-22 eCollection Date: 2025-12-01 DOI: 10.1007/s12551-025-01387-7

Wilma K Olson

引用次数: 0

Studying the Super-Relaxed (SRX) state in cardiac and skeletal muscle: a protocol-based perspective from fibers to fibrils. 研究心脏和骨骼肌的超放松（SRX）状态：从纤维到原纤维的协议视角。

IF 5

Biophysical reviews Pub Date : 2025-11-22 eCollection Date: 2026-06-01 DOI: 10.1007/s12551-025-01382-y

Nariman Naber, Clyde Wilson, Cristobal Dos Remedios, Filip Braet, Amy Li

{"title":"Studying the Super-Relaxed (SRX) state in cardiac and skeletal muscle: a protocol-based perspective from fibers to fibrils.","authors":"Nariman Naber, Clyde Wilson, Cristobal Dos Remedios, Filip Braet, Amy Li","doi":"10.1007/s12551-025-01382-y","DOIUrl":"https://doi.org/10.1007/s12551-025-01382-y","url":null,"abstract":"The super-relaxed state (SRX) of myosin plays a key role in maintaining muscle efficiency and regulating metabolic rate. In this state, myosin heads exhibit significantly reduced ATPase activity, resulting in lower energy consumption when muscles are at rest. SRX is in dynamic equilibrium with the more disordered relaxed state (DRX), allowing shifts between these states to profoundly influence muscle function and energy expenditure. This deeper understanding of SRX, and its ability to be modulated has important implications for treating cardiac and skeletal muscle disorders where contractile function is impaired. Here, we present a detailed experimental protocol for studying SRX in cardiomyocytes and skeletal muscle fibers developed by Roger Cooke for different species, and adapted for our understanding of SRX biophysics. The protocol encompasses muscle dissection, data analysis, key reagents, auxiliary lab tools, notes, and tips that help with reproducibility and troubleshooting challenges. We hope this contribution will serve as a resource and inspiration for the next generation of scientists to explore muscle function and apply their findings toward improving human health.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"18 3","pages":"601-611"},"PeriodicalIF":5.0,"publicationDate":"2025-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13486416/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148788131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A mathematical description of the convectional reaction-diffusion of metabolites in a biological system. 对生物系统中代谢物的对流反应扩散的数学描述。

IF 3.7

Biophysical reviews Pub Date : 2025-11-14 eCollection Date: 2025-10-01 DOI: 10.1007/s12551-025-01366-y

Yaroslav R Nartsissov

{"title":"A mathematical description of the convectional reaction-diffusion of metabolites in a biological system.","authors":"Yaroslav R Nartsissov","doi":"10.1007/s12551-025-01366-y","DOIUrl":"https://doi.org/10.1007/s12551-025-01366-y","url":null,"abstract":"The process of reaction-diffusion combined with advection plays a crucial role in biological systems. It establishes concentration gradients inside the cells, which in turn facilitates the regulation of signaling and metabolic activities. Theoretical descriptions of these processes make use of boundary problems linked to parabolic partial differential equations. This review focuses on the key biological characteristics that contribute to the formulation of the governing equation. The explanation of biochemical processes and transport mechanisms confirms the validity of both the terms in the equations and the established boundary conditions. In the context of real biological objects, a meticulous description of the modeled area is a fundamental requirement. Therefore, special care is taken in the formulation of algorithms that facilitate the creation of three-dimensional digital phantoms. The concept of phantom creation was illustrated through the application of 3D Voronoi diagrams. Indeed, in biological systems, fluid dynamics also play a crucial role, enabling the characterization of advection via the solution of boundary problems associated with the Navier-Stokes equation or through the application of the Darcy law in porous media. Furthermore, the convection velocity field is incorporated into the reaction-diffusion equation, and efforts are made to determine the solutions through numerical methods. The results acquired can serve to illustrate multiple biological phenomena. The capabilities of COMSOL Multiphysics software in relation to the biological aspects of reaction-diffusion are also discussed.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"17 5","pages":"1265-1282"},"PeriodicalIF":3.7,"publicationDate":"2025-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12847562/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146084087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protein nanoparticles control bio-osmotic pressure via electromechanical collaboration. 蛋白质纳米颗粒通过机电协同控制生物渗透压。

IF 5

Biophysical reviews Pub Date : 2025-11-08 eCollection Date: 2026-04-01 DOI: 10.1007/s12551-025-01380-0

Yingfei Wang, Zihui Zheng, Jun Guo

{"title":"Protein nanoparticles control bio-osmotic pressure via electromechanical collaboration.","authors":"Yingfei Wang, Zihui Zheng, Jun Guo","doi":"10.1007/s12551-025-01380-0","DOIUrl":"https://doi.org/10.1007/s12551-025-01380-0","url":null,"abstract":"Osmotic pressure (OP) is widely recognized as a crucial driving force for the flow of body fluids. To provide a reasonable explanation for osmosis-dependent pathophysiological phenomena in live cells, we establish fluorescence resonance energy transfer (FRET)-based intermediate filament (IF) tension probes, converting the osmotic effect into optical signals. We then propose the theory of bio-osmotic pressure (bio-OP), which relies on the selective permeability of ion channels and water flux. Protein nanoparticles (PNs) play a crucial role in modulating the transmembrane osmotic gradient by regulating the electrical activity of the plasma membrane and voltage-dependent ion channels. Different ion compositions exhibit synergistic or antagonistic effects on PN-induced electrical activity. As a result, PNs are crucial for maintaining the intracellular osmotic effect. PNs, in collaboration with changes in ions and water, establish a new homeostasis in membrane potential and bio-OP. This collaboration led to the synergistic regulation of electromechanical activity involved in the occurrence and development of various diseases associated with bio-OP, such as brain edema. Elucidation of the mechanism underlying bio-OP can provide a novel viewpoint on the pathophysiology of various complex diseases. Hence, further research on bio-OP needs to be conducted.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"18 2","pages":"499-509"},"PeriodicalIF":5.0,"publicationDate":"2025-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13272718/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148276028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamics of natural biological membranes: an NMR history. 天然生物膜动力学：核磁共振历史。

IF 3.7

Biophysical reviews Pub Date : 2025-11-03 eCollection Date: 2025-12-01 DOI: 10.1007/s12551-025-01375-x

Erick J Dufourc, Estelle Morvan

引用次数: 0

Information optimality and the geometry of chromatin. 信息最优性和染色质的几何结构。

IF 5

Biophysical reviews Pub Date : 2025-11-03 eCollection Date: 2026-04-01 DOI: 10.1007/s12551-025-01374-y

Subhash Kak

引用次数: 0

Advanced optical microscopy methods for in situ single-molecule studies of membrane proteins. 膜蛋白原位单分子研究的先进光学显微镜方法。

IF 3.7

Biophysical reviews Pub Date : 2025-10-25 eCollection Date: 2025-08-01 DOI: 10.1007/s12551-025-01372-0

Shannan Foylan, Gail McConnell, Gwyn W Gould

{"title":"Advanced optical microscopy methods for in situ single-molecule studies of membrane proteins.","authors":"Shannan Foylan, Gail McConnell, Gwyn W Gould","doi":"10.1007/s12551-025-01372-0","DOIUrl":"10.1007/s12551-025-01372-0","url":null,"abstract":"Integral membrane proteins are crucial molecules ubiquitous to all cell types, coordinating cell signalling and facilitating the tightly regulated transport of essential nutrients across plasma membrane. Defects in membrane proteins are associated with disease, emphasising the need to understand the structural, mechanistic and regulatory mechanisms which control integral membrane proteins. Recent technological advances in optical microscopy have allowed appropriate study of these small proteins using tools with molecular resolution which can non-invasively observe their native organisation in the plasma membrane in situ. Complimentarily, by utilising photochemical phenomena and analyses, single-molecule detail can be elucidated from conventional microscope systems. In this review, we firstly overview the methodologies used for studies of membrane proteins and then review the biophysical results gleaned from their application with an emphasis on membrane transporters. We show that single molecule studies of integral membrane proteins are beginning to unveil striking new regulatory mechanisms with wide applicability across many distinct fields of biological research.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"17 4","pages":"991-1011"},"PeriodicalIF":3.7,"publicationDate":"2025-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12686294/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145720860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface-dependent reactions in blood coagulation and fibrinolysis: beyond procoagulant platelets. 凝血和纤溶中的表面依赖性反应：超越促凝血小板。

IF 5

Biophysical reviews Pub Date : 2025-10-23 eCollection Date: 2025-10-01 DOI: 10.1007/s12551-025-01376-w

Jiangguo Lin, Kirill R Butov, Anastasia N Sveshnikova, Stepan Gambaryan, Mikhail A Panteleev

{"title":"Surface-dependent reactions in blood coagulation and fibrinolysis: beyond procoagulant platelets.","authors":"Jiangguo Lin, Kirill R Butov, Anastasia N Sveshnikova, Stepan Gambaryan, Mikhail A Panteleev","doi":"10.1007/s12551-025-01376-w","DOIUrl":"10.1007/s12551-025-01376-w","url":null,"abstract":"The essential biochemical processes of blood coagulation and fibrinolysis during thrombus formation do not occur in the liquid phase but are instead restricted to specific surfaces. The classic example is phosphatidylserine-containing membranes of procoagulant platelets, which can accelerate the membrane-dependent coagulation reactions by several orders of magnitude. However, there is no clear evidence that this acceleration is the only-or even the primary-consequence of the coagulation factors' binding to the procoagulant membranes. Furthermore, other important surfaces have been identified, including fibrin (together with its numerous associated proteins) and phosphate-rich polymers such as platelet-derived polyphosphates and neutrophil extracellular traps. The distribution of these surfaces within a thrombus is non-uniform, forming complex structures at both micro- and macro-scale. This review explores possible hypotheses regarding their physiological and pathological roles: modulation of the reaction kinetics; regulation of transport processes depending on the rheological microenvironment of the thrombus; integration of coagulation, platelet activity, fibrinolysis, and tissue repair; and control of clot mechanical properties. Elucidating and validating these mechanisms may provide new insights into the development of therapeutic and diagnostic strategies.","PeriodicalId":9094,"journal":{"name":"Biophysical reviews","volume":"17 5","pages":"1543-1556"},"PeriodicalIF":5.0,"publicationDate":"2025-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12847512/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146084199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0