ISRN biotechnology最新文献_第3页

Application of Plackett-Burman Experimental Design for Lipase Production by Aspergillus niger Using Shea Butter Cake. Plackett-Burman试验设计在黑曲霉利用乳木果蛋糕生产脂肪酶中的应用。

ISRN biotechnology Pub Date : 2013-02-13 eCollection Date: 2013-01-01 DOI: 10.5402/2013/718352

Aliyu Salihu, Muntari Bala, Shuaibu M Bala

引用次数: 30

Differential Inhibition of Helicoverpa armigera (Hubner) Gut Proteinases by Proteinase Inhibitors of Okra and It's Wild Relatives. 秋葵及其野生近缘种蛋白酶抑制剂对棉铃虫肠道蛋白酶的差异抑制

ISRN biotechnology Pub Date : 2013-02-03 eCollection Date: 2013-01-01 DOI: 10.5402/2013/632173

Shilpa K Udamale, M P Moharil, T B Ugale, J M Mankar

{"title":"Differential Inhibition of Helicoverpa armigera (Hubner) Gut Proteinases by Proteinase Inhibitors of Okra and It's Wild Relatives.","authors":"Shilpa K Udamale, M P Moharil, T B Ugale, J M Mankar","doi":"10.5402/2013/632173","DOIUrl":"https://doi.org/10.5402/2013/632173","url":null,"abstract":"The seeds of ten genotypes and twenty-nine wild relatives of okra were analysed for the presence of trypsin, chymotrypsin, and Helicoverpa gut proteinases (HGPs) inhibitors (HGPIs), with the aim to identify potent inhibitors of H. armigera gut proteinases. Proteinase inhibitors (PIs) obtained from wild relatives of okra exhibited stronger inhibition of HGPs than the genotypes of okra. In in vitro inhibitory assay against HGPs, A. tuberculatus 90396 and 90515 showed high tryptic inhibitory (71.8% and 69.2%), chymotryptic inhibitory (68.5% and 66.2%), and Helicoverpa gut proteinase activity (70.2% and 68.2%). In electrophoretic profile showed the same variation in the number of trypsin inhibitors (TIs), chymotrypsin Inhibitors (CIs), and HGPIs isoforms with different intensities, whereas genotypes of okra mostly showed monomorphic profile. Maximum eight HGPIs isoforms were found in A. tuberculatus (90396 and 90515). In bioassay studies, significant reduction in weight of H. armigera larvae was found, when larvae fed on PIs obtained from A. tuberculatus (90396 and 90515). Thus, the result of the present investigation indicates that further exploration of PIs obtained from A. tuberculatus (90396 and 90515) will be helpful for developing PIs-based insect resistance management strategies. ","PeriodicalId":90878,"journal":{"name":"ISRN biotechnology","volume":"2013 ","pages":"632173"},"PeriodicalIF":0.0,"publicationDate":"2013-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4393048/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33273626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Identification of Appropriate Reference Genes for qRT-PCR Analysis of Heat-Stressed Mammary Epithelial Cells in Riverine Buffaloes (Bubalus bubalis). 水牛热应激乳腺上皮细胞qRT-PCR分析内参基因的鉴定

ISRN biotechnology Pub Date : 2013-01-28 eCollection Date: 2013-01-01 DOI: 10.5402/2013/735053

Neha Kapila, Amit Kishore, Monika Sodhi, Ankita Sharma, Pawan Kumar, A K Mohanty, Tanushri Jerath, M Mukesh

引用次数: 25

Yield and properties of ethanol biofuel produced from different whole cassava flours. 不同全木薯粉生产乙醇生物燃料的产量和性质。

ISRN biotechnology Pub Date : 2013-01-21 eCollection Date: 2013-01-01 DOI: 10.5402/2013/916481

F T Ademiluyi, H D Mepba

{"title":"Yield and properties of ethanol biofuel produced from different whole cassava flours.","authors":"F T Ademiluyi, H D Mepba","doi":"10.5402/2013/916481","DOIUrl":"https://doi.org/10.5402/2013/916481","url":null,"abstract":"The yield and properties of ethanol biofuel produced from five different whole cassava flours were investigated. Ethanol was produced from five different whole cassava flours. The effect of quantity of yeast on ethanol yield, effect of whole cassava flour to acid and mineralized media ratio on the yield of ethanol produced, and the physical properties of ethanol produced from different cassava were investigated. Physical properties such as distillation range, density, viscosity, and flash point of ethanol produced differ slightly for different cultivars, while the yield of ethanol and electrical conductivity of ethanol from the different cassava cultivars varies significantly. The variation in mineral composition of the different whole cassava flours could also lead to variation in the electrical conductivity of ethanol produced from the different cassava cultivars. The differences in ethanol yield are attributed to differences in starch content, protein content, and dry matter of cassava cultivars. High yield of ethanol from whole cassava flour is best produced from cultivars with high starch content, low protein content, and low fiber. ","PeriodicalId":90878,"journal":{"name":"ISRN biotechnology","volume":"2013 ","pages":"916481"},"PeriodicalIF":0.0,"publicationDate":"2013-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5402/2013/916481","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33146729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 35

Variations in the Regulatory Region of Alpha S1-Casein Milk Protein Gene among Tropically Adapted Indian Native (Bos Indicus) Cattle. 热带适应印度土著牛α s1 -酪蛋白乳蛋白基因调控区域的变异

ISRN biotechnology Pub Date : 2013-01-14 eCollection Date: 2013-01-01 DOI: 10.5402/2013/926025

Amit Kishore, Manishi Mukesh, Ranbir C Sobti, Bishnu P Mishra, Monika Sodhi

{"title":"Variations in the Regulatory Region of Alpha S1-Casein Milk Protein Gene among Tropically Adapted Indian Native (Bos Indicus) Cattle.","authors":"Amit Kishore, Manishi Mukesh, Ranbir C Sobti, Bishnu P Mishra, Monika Sodhi","doi":"10.5402/2013/926025","DOIUrl":"https://doi.org/10.5402/2013/926025","url":null,"abstract":"Regulatory region of milk protein alpha S1-casein (αS1-CN) gene was sequenced, characterized, and analyzed to detect variations among 13 Indian cattle (Bos indicus) breeds. Comparative analysis of 1,587 bp region comprising promoter (1,418 bp), exon-I (53 bp), and partial intron-I (116 bp) revealed 35 nucleotide substitutions (32 within promoter region, 1 in exon-I, and 2 in partial intron-I region) and 4 Indels. Within promoter, 15 variations at positions -1399 (A > G), -1288 (G > A), -1259 (T > C), -1158 (T > C), -1016 (A > T), -941 (T > G), -778 (C > T), -610 (G > A), -536 (A > G), -521 (A > G), -330 (A > C), -214 (A > G), -205 (A > T), -206 (C > A), and -175 (A > G) were located within the potential transcription factor binding sites (TFBSs), namely, NF-κE1/c-Myc, GATA-1, GATA-1/NF-E, Oct-1/POU3F2, MEF-2/YY1, GATA-1, AP-1, POU1F1a/GR, TMF, GAL4, YY1/Oct-1, HNF-1, GRalpha/AR, GRalpha/AR, and AP-1, respectively. Seventy-four percent (26/35) of the observed SNPs were novel to Indian cattle and 11 of these novel SNPs were located within one or more TFBSs. Collectively, these might influence the binding affinity towards their respective nuclear TFs thus modulating the level of transcripts in milk and affecting overall protein composition. The study provides information on several distinct variations across indicine and taurine αS1-CN regulatory domains. ","PeriodicalId":90878,"journal":{"name":"ISRN biotechnology","volume":"2013 ","pages":"926025"},"PeriodicalIF":0.0,"publicationDate":"2013-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4393073/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33146730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Linen most useful: perspectives on structure, chemistry, and enzymes for retting flax. 亚麻最有用:亚麻的结构、化学和酶的观点。

ISRN biotechnology Pub Date : 2012-12-30 eCollection Date: 2013-01-01 DOI: 10.5402/2013/186534

Danny E Akin

{"title":"Linen most useful: perspectives on structure, chemistry, and enzymes for retting flax.","authors":"Danny E Akin","doi":"10.5402/2013/186534","DOIUrl":"https://doi.org/10.5402/2013/186534","url":null,"abstract":"The components of flax (Linum usitatissimum) stems are described and illustrated, with reference to the anatomy and chemical makeup and to applications in processing and products. Bast fiber, which is a major economic product of flax along with linseed and linseed oil, is described with particular reference to its application in textiles, composites, and specialty papers. A short history of retting methods, which is the separation of bast fiber from nonfiber components, is presented with emphasis on water retting, field retting (dew retting), and experimental methods. Past research on enzyme retting, particularly by the use of pectinases as a potential replacement for the current commercial practice of field retting, is reviewed. The importance and mechanism of Ca(2+) chelators with pectinases in retting are described. Protocols are provided for retting of both fiber-type and linseed-type flax stems with different types of pectinases. Current and future applications are listed for use of a wide array of enzymes to improve processed fibers and blended yarns. Finally, potential lipid and aromatic coproducts derived from the dust and shive waste streams of fiber processing are indicated. ","PeriodicalId":90878,"journal":{"name":"ISRN biotechnology","volume":"2013 ","pages":"186534"},"PeriodicalIF":0.0,"publicationDate":"2012-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4403609/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33300255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 87

Challenges in enzymatic route of mannitol production. 甘露醇酶法生产的挑战。

ISRN biotechnology Pub Date : 2012-12-26 eCollection Date: 2013-01-01 DOI: 10.5402/2013/914187

Sheelendra Mangal Bhatt, Anand Mohan, Suresh Kumar Srivastava

引用次数: 23

Induction of Defense-Related Enzymes in Banana Plants: Effect of Live and Dead Pathogenic Strain of Fusarium oxysporum f. sp. cubense. 香蕉植物防御相关酶的诱导:香蕉尖孢镰刀菌生、死致病菌株的影响。

ISRN biotechnology Pub Date : 2012-12-06 eCollection Date: 2013-01-01 DOI: 10.5402/2013/601303

Janki N Thakker, Samiksha Patel, Pinakin C Dhandhukia

{"title":"Induction of Defense-Related Enzymes in Banana Plants: Effect of Live and Dead Pathogenic Strain of Fusarium oxysporum f. sp. cubense.","authors":"Janki N Thakker, Samiksha Patel, Pinakin C Dhandhukia","doi":"10.5402/2013/601303","DOIUrl":"https://doi.org/10.5402/2013/601303","url":null,"abstract":"The aim of the present study was to scrutinize the response of banana (Grand Naine variety) plants when interacting with dead or live pathogen, Fusarium oxysporum f.sp. cubense, a causative agent of Panama disease. Response of plants was evaluated in terms of induction of defense-related marker enzyme activity, namely, peroxidase (POX), polyphenol oxidase (PPO), β-1,3 glucanase, chitinase, and phenolics. Plant's interaction with live pathogen resulted in early induction of defense to restrain penetration as well as antimicrobial productions. However, pathogen overcame the defense of plant and caused disease. Interaction with dead pathogen resulted in escalating defense response in plants. Later on plants inoculated with dead pathogen showed resistance to even forced inoculation of live pathogen. Results obtained in the present study suggest that dead pathogen was able to mount defense response in plants and provide resistance to Panama disease upon subsequent exposure. Therefore, preparation from dead pathogen could be a potential candidate as a biocontrol agent or plant vaccine to combat Panama disease. ","PeriodicalId":90878,"journal":{"name":"ISRN biotechnology","volume":"2013 ","pages":"601303"},"PeriodicalIF":0.0,"publicationDate":"2012-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5402/2013/601303","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33300169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 65

Development of biological oxygen demand biosensor for monitoring the fermentation industry effluent. 用于发酵工业废水监测的生物需氧量传感器的研制。

ISRN biotechnology Pub Date : 2012-11-27 eCollection Date: 2013-01-01 DOI: 10.5402/2013/236062

Neelam Verma, Ashish Kumar Singh

引用次数: 23

Biosynthesis of Gold Nanoparticles Using Fusarium oxysporum f. sp. cubense JT1, a Plant Pathogenic Fungus. 植物病原真菌尖孢镰刀菌JT1合成纳米金的研究。

ISRN biotechnology Pub Date : 2012-11-11 eCollection Date: 2013-01-01 DOI: 10.5402/2013/515091

Janki N Thakker, Pranay Dalwadi, Pinakin C Dhandhukia

引用次数: 49