BMC Oral Health最新文献

{"title":"Presence of TMD-related pain and symptoms associated with anxiety in Peruvian students in their final years of dental education: an analytical cross-sectional study under a multivariable regression model.","authors":"Karen Angeles-García, Marysela Ladera-Castañeda, Leonor Castro-Ramirez, Elizabeth Paucar-Rodríguez, Miriam Castro-Rojas, Luis Cervantes-Ganoza, César Cayo-Rojas","doi":"10.1186/s12903-025-05638-7","DOIUrl":"10.1186/s12903-025-05638-7","url":null,"abstract":"<p><strong>Background: </strong>Temporomandibular disorders (TMD) are frequently associated with anxiety, as this can increase the hyperactivity of the masticatory muscles, resulting in TMD-related pain and symptoms. The aim of this study was to assess the presence of TMD-related pain and symptoms associated with anxiety levels in Peruvian students in their final years of dental education.</p><p><strong>Methods: </strong>This analytical cross-sectional study of 273 Peruvian students in the final two years of dental education was conducted from October to December 2023. The Zung test was employed to diagnose anxiety, while the TMD-Pain Screener questionnaire was utilized to diagnose painful temporomandibular disorders (TMD). A Poisson regression model with robust variance using Adjusted Prevalence Ratio (APR) was employed to assess the prevalence of TMD-related pain and symptoms. The following variables were considered in the analysis: anxiety, sex, age, academic year, marital status, area of residence, type of housing, living with parents, and occupation. All statistical tests were conducted with a significance level of p < 0.05.</p><p><strong>Results: </strong>The prevalence of TMD-related pain and symptoms was 24.5%. The 0.7% of the sample exhibited very extreme levels of anxiety; 8.1% demonstrated severe anxiety; and 39.9% exhibited mild to moderate anxiety. Furthermore, dental students with severe to very extreme anxiety and with mild to moderate anxiety were 8.2 times and 3.8 times, respectively, more likely to present TMD-related pain and symptoms (APR = 8.18, 95% CI: 4.62-14.47 and APR = 3.84, 95% CI: 2.18-6.75, respectively), compared to those who did not have anxiety. Conversely, no significant association was observed between the presence of TMD-related pain and symptoms and sex, age, academic year, marital status, area of residence, type of housing, living with parents, or occupation (p > 0.05).</p><p><strong>Conclusion: </strong>Almost a quarter of the students in their final years of dental education had TMD-related pain and symptoms. It was found that as the level of anxiety increased, from mild to moderate and from severe to very extreme, the likelihood of experiencing TMD-related pain and symptoms also increased significantly. On the other hand, gender, age, academic year, marital status, area of residence, type of housing, living with parents or occupation were not found to be influential factors in the presence of TMD-related pain and symptoms.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"277"},"PeriodicalIF":2.6,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11846219/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143472120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of conventional and modified sling suture techniques in free gingival graft operations-a randomized controlled clinical trial.","authors":"Sanubar Shakiliyeva, Demet Sahin, Sadiye Gunpinar, Mihtikar Gursel","doi":"10.1186/s12903-025-05456-x","DOIUrl":"10.1186/s12903-025-05456-x","url":null,"abstract":"<p><strong>Background: </strong>The aim of this study was to investigate the effects of conventional suture (CS) and modified sling suture (MSS) techniques, applied in free gingival graft (FGG) surgery using gingival unit graft (GUT) and conventional graft (CG) techniques, on clinical parameters and graft dimensions.</p><p><strong>Methods: </strong>52 individuals having Cairo Type 2 (RT2) and Type 3 (RT3) gingival recessions in mandibular anterior were divided into four groups as (a) GUT + MSS (n = 13), (b) GUT + CS (n = 13), (c) CG + MSS (n = 13) and (d) CG + CS (n = 13). Keratinized tissue width (KTW), relative gingival recession height (rGRH), and relative vestibule depth (rVD) measurements were recorded using a digital caliper and UNC 15 periodontal probe. Dimensional changes (Δ) of the graft surface area (GSA) was determined by ImageJ software. All measurements were done at baseline, 1st and 3rd months.</p><p><strong>Results: </strong>It was determined that the increase in KTW in the 1st and 3rd months in GUT + CS group was significantly higher than GUT + MSS group (p < 0.05). There was no statistically significant difference in Δ rVD and Δ rGRH values between the groups. When ΔGSA (mm2) values were compared between the groups, the decrease in the 1st and 3rd months in the CG + MSS group was found to be significantly higher than the GUT + MSS and GUT + CS groups (p < 0.05).</p><p><strong>Conclusion: </strong>Within the limits of this study, it can be concluded that keratinized tissue can be obtained successfully with both GUT or CG techniques in FGG surgery, on the other hand, regardless of the suture technique, GUT showed less graft shrinkage than CG.</p><p><strong>Trial registration: </strong>The study was retrospectively registered at ClinicalTrials (Registration number: NCT06197893; Date of registration: 11 January 2024).</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"279"},"PeriodicalIF":2.6,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11846402/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143472114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of age and systemic inflammation on the association between severity of periodontitis and blood pressure in periodontitis patients.","authors":"Hongjia Yang, Yixuan Qin, Jiaqi Geng, Jinyue Qu, Qian Cheng, Kuibao Li, Dongxue Zhang","doi":"10.1186/s12903-025-05665-4","DOIUrl":"10.1186/s12903-025-05665-4","url":null,"abstract":"<p><strong>Background: </strong>Periodontitis is a periodontal inflammatory disease closely related to hypertension. However, the role of age and systemic inflammation is unclear. This study examined the effect of age and systemic inflammation on the association between severity of periodontitis and blood pressure in periodontitis patients.</p><p><strong>Methods: </strong>This cross-sectional clinical study included periodontitis patients treated at Beijing Chaoyang Hospital, Capital Medical University from January 2020 to July 2023. Patients with periodontitis were included using inclusion and exclusion criteria, and data on basic information, blood pressure, periodontal examination indicators, and biochemical examination indicators were collected. Patients were grouped by blood pressure, and differences in basic information, blood indexes, and periodontal examination indexes between the two groups were compared by independent sample T-test. With PBOP as independent variables, systolic blood pressure (SBP) and diastolic blood pressure as dependent variables, and several indexes as covariables, the relationship between them was studied by linear regression. The interaction was used to explore the relationship between age and systemic inflammation in periodontitis and hypertension.</p><p><strong>Results: </strong>A total of 144 patients with periodontitis were enrolled. In fully adjusted analyses, the percentage of site with bleeding on probing (PBOP) was significantly associated with SBP (B = 10.08, p = 0.027) in the overall group. In patients 50 years or older, but not in those younger than 50, PBOP remained significantly associated with SBP (p = 0.018 for interaction). The strength of association between PBOP and SBP was greater in patients with higher systemic immune-inflammation index. The regression coefficients of PBOP in the systemic immune-inflammation index ≥ 400 and < 400 groups were 16.61 (95% CI, 3.32-29.90; p = 0.015) and 13.69 (95% CI, 1.15-26.23; p = 0.033), respectively (p = 0.047 for interaction). Similar results were observed in the systemic inflammation response index ≥ 9 and < 9 groups (B = 17.04 (95% CI, 2.66-31.42, p = 0.021) and 13.98 (95% CI, 1.94-26.01, p = 0.024 respectively; p = 0.028 for interaction).</p><p><strong>Conclusions: </strong>This study indicates a significant association between the severity of periodontitis and SBP, which was modified by age and systemic inflammation.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"273"},"PeriodicalIF":2.6,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11843814/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143472116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined use of deproteinized bovine bone mineral and α-tricalcium phosphate using gelatin carriers.","authors":"Masako Fujioka-Kobayashi, Veronika Urbanova, Niklaus P Lang, Hiroki Katagiri, Nikola Saulacic","doi":"10.1186/s12903-025-05644-9","DOIUrl":"10.1186/s12903-025-05644-9","url":null,"abstract":"<p><strong>Objective: </strong>To study the effect on biomaterial degradation and bone formation of different ratios between α-tricalcium phosphate (α-TCP) and deproteinized bovine bone mineral (DBBM) using various gelatins as a carrier.</p><p><strong>Materials and methods: </strong>Thirty-six critical-sized calvarial bone defects were randomly treated in 18 animals. Four biomaterials with different compositional relations of DBBM to α-TCP and granules to carrier were investigated: (1) 40 ± 10% DBBM/40 ± 10% α-TCP with 20 ± 10% gelatin type 1 in ratio 4:1 (B1/G1), (2) 20 ± 10% DBBM/60 ± 10% α-TCP with 20 ± 10% gelatin type 1 in ratio 4:1 (B2/G1), (3) 20 ± 10% DBBM/60 ± 10% α-TCP with 15 ± 10%/5 ± 5% gelatin type 2/ glycerine (B2/G2) and 4), 10 ± 10% DBBM/60 ± 10% α-TCP with 20 ± 10%/10 ± 10% gelatin type 2/ glycerine (B3/G2). As a positive control 50 ± 10% DBBM/50 ± 10% α-TCP without gelatin (PC, B1/G0) and as a negative control (NC) empty defects were chosen. All defects were covered with a collagen membrane. The samples were harvested 4 weeks post-surgically and examined by micro-CT and histomorphometric analysis.</p><p><strong>Results: </strong>New bone formation was evident in all defects. The mineralized tissue volume was significantly higher in the four tested biomaterials than in the NC group, but lower compared to the PC group. Histomorphometry showed similar levels of bone formation in all groups, whereas only the PC group reached a significantly higher total augmentation area than that of the NC. The PC group showed significantly higher mineralized tissue density and residual material area compared to the B3/G2 group, and more residual DBBM than the four tested biomaterials.</p><p><strong>Conclusions: </strong>New bone formation was not significantly affected either by different DBBM:α-TCP compositional ratios nor the presence of various gelatin carriers.</p><p><strong>Clinical relevance: </strong>Similar levels of osteoconductivity indicates the presumptive use of combined products in alveolar ridge augmentation to support bone formation. Gelatin with or without glycerine may be considered for its use as a carrier to the biomaterials frequently applied in peri-implant surgery.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"275"},"PeriodicalIF":2.6,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11846255/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143472257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of psychological stress on salivary cortisol levels in periodontitis patients: a case-control study.","authors":"Mozaffer Rahim Hingorjo, Muhammad Owais, Saad Uddin Siddiqui, Shazia Nazar, Yumna Shaheen Ali","doi":"10.1186/s12903-024-05017-8","DOIUrl":"10.1186/s12903-024-05017-8","url":null,"abstract":"<p><strong>Background: </strong>Psychological stress is linked to elevated salivary cortisol levels, potentially worsening periodontitis by exacerbating inflammation. This study examines the impact of stress on cortisol levels in periodontitis patients, aiming to explore salivary cortisol as a biomarker for disease severity and its role in improving management strategies.</p><p><strong>Methods: </strong>This case-control study, conducted at Dow University of Health Sciences in Karachi from January to December 2022, involved 120 dental clinic patients aged 30-60. Participants were selected based on the presence (cases) or absence (controls) of periodontitis, excluding those with systemic diseases, taking hydrocortisone, immunosuppressives, antidepressants, systemic antibiotics, having oral ulcers and pregnancy or lactation. Periodontal parameters, stress levels (using the Perceived Stress Scale), and morning salivary cortisol (measured via ELISA) were assessed. Statistical analyses were performed using SPSS version 23, including descriptive statistics, independent t-tests, ANOVA, and odds ratio calculations with a 95% confidence interval at 0.05.</p><p><strong>Results: </strong>The study cohort comprised 120 subjects, equally divided between periodontitis patients and healthy controls. Predominantly male (63%) with a mean age of 41.75 years, participants were subjected to comprehensive evaluations. Oral health indices (Gingival index, Plaque index, Clinical attachment loss, Periodontal probing depth, Tooth mobility) and stress markers (Perceived Stress Score, Salivary cortisol levels [SCLs]) were significantly higher in cases compared to controls (p < 0.001). Majority of cases (58.3%) had stage II periodontitis. Notably, cases exhibited significantly higher stress levels (2.05 ± 0.59 vs. 1.38 ± 0.52; p < 0.001) and elevated salivary cortisol levels (6.67 ± 1.64 vs. 2.54 ± 0.88; p < 0.001) compared to controls. The odds ratio (OR) of 3.73 (95% CI [1.75, 7.93], p = 0.0006) indicated that periodontitis patients were over 3.5 times more likely to have elevated SCLs. Stress prevalence was 42.5% in periodontitis patients compared to 18.33% in controls.</p><p><strong>Conclusions: </strong>This study highlights a potential link between stress, cortisol levels, and periodontitis, suggesting that salivary cortisol could be a valuable biomarker. Incorporating cortisol measurements into routine dental evaluations may enable personalized treatment plans, addressing both biological and psychological contributors to periodontitis. Further research is needed to explore the long-term effects of stress on cortisol levels and periodontal disease progression, as well as the role of stress management in managing periodontitis.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"276"},"PeriodicalIF":2.6,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11846471/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143472126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the capabilities of GenAI for oral cancer consultations in remote consultations : Author.","authors":"Yu-Tao Xiong, Hao-Nan Liu, Yu-Min Zeng, Zheng-Zhe Zhan, Wei Liu, Yuan-Chen Wang, Wei Tang, Chang Liu","doi":"10.1186/s12903-025-05619-w","DOIUrl":"10.1186/s12903-025-05619-w","url":null,"abstract":"<p><strong>Background: </strong>Generative artificial intelligence (GenAI) has demonstrated potential in remote consultations, yet its capacity to comprehend oral cancer has not yet been fully evaluated. The objective of this study was to evaluate the accuracy, reliability and validity of GenAI in addressing questions related to remote consultations for oral cancer.</p><p><strong>Methods: </strong>A search was conducted on telemedicine platforms in China, summarizing patients' inquiries regarding oral cancer. A panel of board-certified oral surgeons compiled the reference answers for addressing these questions. GPT-3.5-turbo and GPT-4o were tasked to answer specific questions related to oral cancer, with their responses recorded. The responses were assessed using qualitative and quantitative measures, including the accuracy, the number of key points, text length, lexical density, and a Likert scale. The chi-square test was utilized to detect differences in qualitative data, while Kruskal-Wallis test, Mann-Whitney U test and t-test for quantitative data.</p><p><strong>Results: </strong>A total of 34 oral cancer questions were included, covering basic, etiology, diagnosis, intervention, and prognosis. GPT-3.5-Turbo demonstrated an overall accuracy rate of 77.50% in qualitative analysis, and GPT-4o was 88.20%. The average scores of GPT-3.5Turbo and GPT-4o were 3.96 and 4.35, respectively, with statistically significant differences. GPT-3.5-Turbo and GPT-4o were close to the reference answers in terms of the number of key points, but significantly lower in terms of text length and lexical density.</p><p><strong>Conclusion: </strong>GPT-4o demonstrated a marginal advantage, although no statistically significant differences in response accuracy were observed between GPT-3.5-Turbo and GPT-4o. Moreover, GPT-4o outperformed in terms of reliability and validity, making it more appropriate for remote consultation scenarios.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"269"},"PeriodicalIF":2.6,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11844040/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a predictive nomogram based on preoperative inflammation-nutrition-related markers for prognosis in locally advanced lip squamous cell carcinoma after surgical treatment.","authors":"Hao Cheng, Xue-Lian Xu, Zheng Zhang, Jin-Hong Xu, Zhuo-Ran Li, Ya-Nan Wang, Bo-Dong Zhang, Ke Chen, Shou-Yu Wang","doi":"10.1186/s12903-025-05663-6","DOIUrl":"10.1186/s12903-025-05663-6","url":null,"abstract":"<p><strong>Background: </strong>The prognostic role of preoperative inflammation-nutrition-related markers in locally advanced lip squamous cell carcinoma (LSCC) remains underexplored. This study aimed to assess the impact of various preoperative inflammation-nutrition-related markers on the prognosis of patients with locally advanced LSCC undergoing surgical treatment and to establish a corresponding predictive model.</p><p><strong>Methods: </strong>A retrospective analysis was performed on the clinical data of 169 patients with locally advanced LSCC who underwent surgical treatment. A total of 27 clinicopathological variables, including inflammation-nutrition-related markers, were collected. Univariate and multivariate Cox regression analyses were used to identify independent prognostic factors for disease-free survival (DFS) and overall survival (OS). The nomogram models were validated using receiver operating characteristic (ROC) curve analysis, calibration plots, and decision curve analysis (DCA). Risk stratification was performed based on the nomogram scores, and differences between risk subgroups were explored.</p><p><strong>Results: </strong>The extranodal extension (ENE), surgical safety margin, Glasgow prognostic score (GPS), Geriatric Nutritional Risk Index (GNRI), Controlling Nutrition score (CONUT), American Joint Committee on Cancer (AJCC) stage, and adjuvant radiotherapy were independent prognostic factors for DFS. In contrast, ENE, surgical safety margin, GNRI, CONUT, AJCC stage, and adjuvant radiotherapy were also independent prognostic factors for OS. The nomograms demonstrated better predictive performance than the AJCC staging system. Based on the nomogram model, patients were stratified into low-, medium-, and high-risk subgroups, which exhibited significant differences in survival outcomes.</p><p><strong>Conclusion: </strong>GPS, GNRI, and CONUT are independent factors affecting the prognosis of patients with locally advanced LSCC undergoing radical surgery. By combining GPS, GNRI, and COUNT with other independent clinicopathological prognostic factors, a reliable nomogram model can be established to accurately predict patients' DFS and OS. This provides a powerful tool for individualized prognostic assessment, optimized risk stratification, and treatment decision-making.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"268"},"PeriodicalIF":2.6,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11843749/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Current situation, trends, and challenges navigating temporary anchorage devices usage among orthodontists in China: a comprehensive multi-center survey.","authors":"Zining Chen, Hao Wang, Qianwen Li, Ningrui Zhao, Jieni Zhang, Xuedong Wang","doi":"10.1186/s12903-025-05649-4","DOIUrl":"10.1186/s12903-025-05649-4","url":null,"abstract":"<p><strong>Background: </strong>The temporary anchorage devices (TADs) are presently a viable and popular treatment option for orthodontics all over the world. However, there is a lack of investigation and research on its application among orthodontists in China. This study aims to explore factors influencing temporary anchorage devices usage and compare current perspectives and usage patterns between orthodontic specialists and general dentists, thereby establishing new consensus and guiding future improvements.</p><p><strong>Methods: </strong>A cross-sectional study using a multi-center structured questionnaire was designed. The Chi-square test, Mann-Whitney U test, and logistic regression were performed.</p><p><strong>Results: </strong>Respondents were from 30 provincial-level regions. Most doctors had relatively rich clinical experience in orthodontics, with 65.70% working for over 10 years. Respondents reported a relatively high usage rate (87.54%) and satisfaction with TADs (93.85%). The analysis result showed Chinese orthodontists who received more TADs training (P < 0.01), had higher educational attainment (P < 0.01), and used more clear aligners (P < 0.01) tended to use TADs more frequently. About 95.90% of respondents believed TADs helped achieve higher treatment goals, 68.23% agreed they could shorten treatment duration, and 51.82% believed they reduced the probability of orthodontic-orthognathic treatment. Differences in the understanding of indications for TADs, improvement method of TADs can be observed between orthodontic specialists and general dentists (P < 0.05).</p><p><strong>Conclusions: </strong>Chinese orthodontists were relatively active in using TADs. The factors most closely associated with TAD usage included related training, the type of orthodontic appliances used and the highest educational attainment. Orthodontic specialists and general dentists might had differing perspectives regarding TADs, but they tended to follow similar management procedures.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"272"},"PeriodicalIF":2.6,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11843738/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential of mir-299-5p to modulate LPS-induced inflammation and osteogenic differentiation of periodontal stem cells by targeting PUM2.","authors":"Qing-Hua Zhang, Yu-Xi Zhang, Ling Zhang","doi":"10.1186/s12903-025-05617-y","DOIUrl":"10.1186/s12903-025-05617-y","url":null,"abstract":"<p><strong>Background: </strong>Periodontitis is a prevalent oral disease that significantly impacts human quality of life. This study aimed at the influence of microRNA-299-5p (miR-299-5p) on regulating lipopolysaccharide (LPS)-induced osteogenic differentiation of periodontal ligament stem cells (PDLSCs).</p><p><strong>Methods: </strong>The 105 periodontitis patients and 102 healthy periodontal volunteers (HC) were recruited with their clinical baseline data. miR-299-5p expression in saliva was measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR). To identify the target relationship between miR-299-5p and pumilio RNA-binding family member 2 (PUM2), the dual luciferase reporter gene was explored. PDLSCs were treated with LPS and subjected to osteogenic differentiation induction. The secretion of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interleukin-1 beta (IL-1β) was determined by enzyme-linked immunosorbent assay. mRNA expression of runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), and osteocalcin (OCN) was detected by qRT-PCR.</p><p><strong>Results: </strong>miR-299-5p expression was markedly reduced in periodontitis patients' saliva and negatively correlated with patients' attachment loss, bleeding index, plaque index, probing pocket depth, and TNF-α, IL-6, and IL-1β levels. miR-299-5p could be well distinguished between periodontitis and HC. LPS induction dramatically stimulated the secretion of inflammatory factors and inhibited the differentiation of PDLSCs, which was counteracted by miR-299-5p overexpression. miR-299-5p was detected to target PUM2, which could exacerbate the inflammatory condition of PDLSCs and lead to differentiation defects.</p><p><strong>Conclusions: </strong>miR-299-5p has emerged as a promising diagnostic marker for periodontitis and may attenuate inflammation and contribute to osteogenic differentiation in PDLSCs cells by targeting PUM2.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"271"},"PeriodicalIF":2.6,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11843968/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of proximal dental caries in primary teeth with a near-infrared-irradiation-assisted intraoral scanner: an in vitro study.","authors":"Abeer K Tashkandi, Sultan O Jiffri, Rahaf M Albalawi, Sara A Albukhari, Sulafa A Mugharbil, Hanin E Yeslam","doi":"10.1186/s12903-025-05629-8","DOIUrl":"10.1186/s12903-025-05629-8","url":null,"abstract":"<p><strong>Background: </strong>The early detection of dental caries is crucial for successful dental care. New intraoral scanners using near-infrared irradiation (NIRI) technology track preventive lesions without ionizing radiation. This study aimed to evaluate the performance of intraoral scanners (IOSs) in detecting proximal caries in primary posterior teeth, compared to conventional methods such as loupes-assisted clinical exams and bitewing (BW) radiography.</p><p><strong>Methods: </strong>Fifteen examined tooth surfaces were used to produce a total of 60 scores by a restorative dentistry consultant (RDC). The tooth surfaces were categorized into caries-free (n = 5 × 4 exam methods) and carious (n = 10 × 4 exam methods) subgroups. Artificial caries lesions were created on specified surfaces and mounted on typodont in sets for evaluation using a simplified modified ICDAS visual and BW radiographic examination, DIAGNOcam device, and iTero Element 5D NIRI-assisted IOS. Reference surface scores were recorded. Investigators (RDCs and recent dental graduates (RDGs)) were trained and calibrated. Inter-examiner agreement, agreement with reference, specificity, and sensitivity were checked.</p><p><strong>Results: </strong>The results showed that the sensitivity and specificity differed between the diagnostic tests. The best agreement of all investigated diagnostic methods with the reference was found using the DIAGNOcam device (ĸ = 0.87) and BW radiography (ĸ = 1.00). High agreement was found for visual examination (by the RDC and RDGs (ĸ ≈ 0.85)) and iTero 5D examination (ĸ = 0.87).</p><p><strong>Conclusions: </strong>The iTero Element 5D IOS had lower sensitivity and specificity compared to other methods. The potential use of IOSs with NIRI as a substitute for conventional diagnostic methods in primary teeth shows promise but requires further investigation.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"270"},"PeriodicalIF":2.6,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11844066/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}