BMC Oral Health最新文献

{"title":"Alveolar bone changes and root resorption in adult patients undergoing mandibular molar distalization with clear aligners: a retrospective CBCT study.","authors":"Ebrahim Ebadi, Pengyu Chen, Zhongyi Xiao, Yi Li, Almustafa Osama, Yukun Gou, Xi Chen, Yunshan Zhao","doi":"10.1186/s12903-025-06857-8","DOIUrl":"10.1186/s12903-025-06857-8","url":null,"abstract":"","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"1570"},"PeriodicalIF":3.1,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12509414/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Costs in dental care: a scoping review of methodologies and trends.","authors":"Pourya Tatari, Reza Rezayatmand, Firoozeh Nilchian","doi":"10.1186/s12903-025-06808-3","DOIUrl":"10.1186/s12903-025-06808-3","url":null,"abstract":"<p><strong>Background: </strong>Oral diseases remain among the most widespread non-communicable conditions globally, disproportionately affecting low- and middle-income populations. Despite the substantial and rising financial burden-estimated at US$ 298 billion in 2010-existing cost studies in dentistry often omit indirect costs and lack methodological consistency. These gaps hinder fair reimbursement, cost-effectiveness modeling, and equitable resource allocation. This scoping review aimed to map current methodologies for dental cost estimation, identify how frequently and by what means overhead costs are incorporated, extract standardized unit costs for common procedures, and assess the sources and valuation techniques underpinning these estimates.</p><p><strong>Methods: </strong>Following an a priori protocol registered with the Isfahan University of Medical Sciences Ethics Committee (Approval Code: IR.MUI.</p><p><strong>Research: </strong>REC.1402.1), we conducted a comprehensive search across six major databases and gray literature sources. Studies were screened using the Participants-Concept-Context (PCC) framework. Eligible studies were original economic evaluations in dentistry that reported detailed cost data. Data extraction was performed using customized charting forms, and all reported costs were standardized to 2024 international dollars (Int'l $) using exchange rates, U.S. Consumer Price Index (CPI) data, and purchasing power parity (PPP) adjustments. Results are reported in accordance with the PRISMA-ScR guidelines.</p><p><strong>Results: </strong>Out of 31,619 retrieved records, 124 studies met inclusion criteria. Most studies were conducted in urban, high-income settings and reported only direct costs (58.1%), while fewer accounted for overheads (41.9%). Advanced allocation techniques such as Activity-Based Costing (ABC) or Time-Driven ABC (TD-ABC) were used in less than 10% of studies. Bottom-up valuation was the predominant approach (72.5%). Unit costs for frequently reported procedures showed wide variation (e.g., surgical tooth removal: Int'l $6-501), driven by methodological and contextual heterogeneity.</p><p><strong>Conclusions: </strong>Despite increasing interest in dental cost estimation, major gaps remain-especially in rural settings, overhead cost inclusion, and methodological transparency. Standardizing costing frameworks, piloting ABC/TD-ABC in real-world settings, and developing open-access dental cost repositories could substantially improve future economic evaluations and reimbursement equity. This review offers a comparative foundation for dentists, researchers, and policymakers to support value-based oral health planning.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"1571"},"PeriodicalIF":3.1,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12509373/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel root canal filling technique using a resin-based sealer without gutta-percha: a summary of two cases.","authors":"Motoki Okamoto, Kiichi Moriyama, Nanako Kuriki, Yusuke Takahashi, Julian G Leprince, Maiko Suzuki, Koji Iwasaki, Henry Fergus Duncan, Mikako Hayashi","doi":"10.1186/s12903-025-06957-5","DOIUrl":"10.1186/s12903-025-06957-5","url":null,"abstract":"<p><strong>Objectives: </strong>Root canal filling is a fundamental step in non-surgical root canal treatment (RCT), required to prevent reinfection or new infection within the root canal system. Traditionally root canal filling uses a sealer in combination with gutta-percha (GP). Here, two cases are presented in which the root canals were filled using only a methacrylic resin-based sealer without GP.</p><p><strong>Materials and methods: </strong>Two patients (one male, one female) required RCT - one due to irreversible pulpitis and the other to facilitate a subsequent post-retained protheses. Each RCT was performed according to a standardized procedure, before root canal filling using only methacrylic resin sealer.</p><p><strong>Results: </strong>Fillings were completed in curved and straight root canals without evidence of apical extrusion. No clinical symptoms or signs of dissolution were observed during follow-up (24 and 12 months in the respective cases).</p><p><strong>Conclusions: </strong>Our findings demonstrate the potential applicability of this novel technique for root canal filling using only a methacrylic resin-based sealer without GP.</p><p><strong>Clinical relevance: </strong>The success of our RCT cases offers evidence to support further clinical studies that compare the outcomes with standard root canal procedures.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"1560"},"PeriodicalIF":3.1,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12505714/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dental outcomes following treatment of pediatric odontogenic cysts: an 18-year retrospective analysis.","authors":"Selen Onat Kayıkcı, Ezgi Ergezen Arık, Gaye Mısırlıoğlu, Caner İncekaş, Seçil Çubuk","doi":"10.1186/s12903-025-06922-2","DOIUrl":"10.1186/s12903-025-06922-2","url":null,"abstract":"<p><strong>Background: </strong>This 18-year retrospective analysis of pediatric odontogenic cysts reviewed characteristics, treatment approaches, and associated risks, including root canal treatment (RCT) and tooth extraction.</p><p><strong>Methods: </strong>The clinical records of patients treated for odontogenic cysts between 2005 and 2023 included demographics, symptoms, radiographic findings, cyst size and location, histopathological diagnoses, treatment methods, and follow-up results were analyzed. Statistical analysis was performed using SPSS, with significance set at p < 0.05.</p><p><strong>Results: </strong>Among the 97 patient records analyzed, there were 43 females (44%), and 54 males (56%) with a mean age of 13.32 ± 3.55. The histopathological diagnosis were dentigerous cysts (n = 58, 60%), followed by radicular cysts (n = 28, 29%) and odontogenic keratocysts (n = 9, 9%), mainly in the posterior mandible. Root canal treatment (RCT) was performed in 18% of the patients, and 72% underwent extraction of at least one permanent tooth. The need for RCT and extraction was higher in larger cysts significantly (p = 0.013). The recurrence rate was low, occurring in 3.1% of with two cases of dentigerous cysts and one odontogenic keratocyst recurring, with an average follow-up of 46 months.</p><p><strong>Conclusion: </strong>The findings suggest that mandibular posterior cysts in the pediatric population often needed tooth extraction, underscoring the importance of early diagnosis by radiographic screening to prevent complications. There is a need for multi-center collaboration and national registries for development of protocols and uniform treatment guidelines in pediatric population.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"1572"},"PeriodicalIF":3.1,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12509377/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salivary and serum levels of high mobility group box protein 1 in periodontitis: a cross-sectional observational study.","authors":"Fatma Oner, Gokhan Ozgenc, Haktan Aslan, Ozlem Guzeltan, Yerda Ozkan Karasu","doi":"10.1186/s12903-025-06954-8","DOIUrl":"10.1186/s12903-025-06954-8","url":null,"abstract":"<p><strong>Background: </strong>High mobility group box protein 1 (HMGB1) exhibits pro-inflammatory functions in chronic inflammatory diseases and is closely associated with other inflammatory markers contributing to sustained inflammatory milieu. This study aimed to evaluate HMGB1 levels and their associations with tumor necrosis factor alpha (TNFα), transforming growth factor beta (TGFβ) and interleukin- 1b (IL-1β) in the saliva and serum of the periodontitis patients.</p><p><strong>Methods: </strong>Sixty-six systematically healthy nonsmoker individuals were enrolled and categorized into three groups: (1) Control, (2) Stage I-II Periodontitis, and (3) Stage III-IV Periodontitis. Comprehensive periodontal assessments were performed. HMGB1, TNFα, IL-1β and TGFβ concentrations were analyzed by enzyme-linked immunosorbent assay (ELISA). One-way ANOVA with Tukey's post-hoc test assessed group differences, and Pearson's correlation evaluated relationships between quantitative variables.</p><p><strong>Results: </strong>Salivary HMGB1 levels were significantly elevated in both periodontitis groups compared to controls (p < 0.0001). Serum HMGB1 levels were significantly higher in the Stage III-IV periodontitis group than in both the control (p = 0.022) and Stage I-II (p < 0.001) periodontitis groups. Serum HMGB1 showed positive correlations with IL-1β levels in both periodontitis groups (p < 0.05 for Stage I-II; p ≤ 0.01 for Stage III-IV). A significant positive correlation was also observed between salivary HMGB1 and TNFα levels in the Stage I-II periodontitis group (p ≤ 0.01).</p><p><strong>Conclusion: </strong>HMGB1 levels increase in saliva and serum as periodontal disease worsens, suggesting enhanced HMGB1 expression was involved in the pathogenetic shifts in the disease process at local and systemic levels.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"1550"},"PeriodicalIF":3.1,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12506375/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sedation applications in pedodontics procedures: which one should we choose?: a retrospective analysis.","authors":"Fatih Oluş, Hüseyin Babun","doi":"10.1186/s12903-025-06871-w","DOIUrl":"10.1186/s12903-025-06871-w","url":null,"abstract":"<p><strong>Background: </strong>The use of sedation in the field of pediatric dentistry is becoming increasingly prevalent. While general anesthesia is perceived as a safer alternative, the appropriate use of sedatives, in conjunction with a well-trained team, can effectively mitigate the risks associated with sedation. Our study aims to identify safe drug combinations for prolonged sedation.</p><p><strong>Methods: </strong>This study was conducted in the Operating Room of the Faculty of Dentistry at Akdeniz University. It encompassed 126 patients, classified as ASA 1-2, aged between 3 and 12 years, who underwent dental treatment with sedation support in the Department of Pedodontics from May to December 2022. The patients were categorized into three groups: (1) Ketamine, (2) Propofol-Ketamine (PK), and (3) Dexmedetomidine-Ketamine (DK). During the procedures, vital signs, the requirement for additional doses, complications, and interventions were meticulously documented. Additionally, the Ramsay Sedation Score (RSS), dentist satisfaction during the procedure, recovery time, and Aldrete scores for the postoperative 1st and 2nd hours were also recorded.</p><p><strong>Results: </strong>There were no statistically significant differences between the groups in terms of age, gender, mean weight, and ASA. The minimum requirement for additional doses was recorded in the DK group. Respiratory depression, apnea, hypoxia, and related maneuvers were most frequently observed in the PK group. DK group had minimum requirement for airway suction event. Hypertension and tachycardia were most frequently observed in the K group, while hypotension and bradycardia were most frequently recorded in the DK group. PONV were most common in the K group in which high ketamine doses were administered. RSS was highest in the DK group. The longest recovery times were observed in the DK group. 1st hour Aldrete scores were lower in the DK group. Dentists reached the highest level of satisfaction in the DK group.</p><p><strong>Conclusions: </strong>This retrospective study provides comparative insights into three sedation regimens for pediatric dental procedures. The dexmedetomidine-ketamine combination demonstrated superior intraoperative stability and fewer respiratory complications, though recovery was prolonged. These findings may guide clinicians in selecting appropriate sedation strategies to improve safety and procedural outcomes in cases involving shared airway management.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"1559"},"PeriodicalIF":3.1,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12505555/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic accuracy of generative large language artificial intelligence models for the assessment of dental crowding.","authors":"Khaled Wafaie, Mohamed E Basyouni, Tanmoy Bhattacharjee, Sabarinath Prasad, Baraa Daraqel, Hisham Mohammed","doi":"10.1186/s12903-025-06960-w","DOIUrl":"10.1186/s12903-025-06960-w","url":null,"abstract":"<p><strong>Background: </strong>Generative artificial intelligence (AI) models have shown potential for addressing text-based dental enquiries and answering exam questions. However, their role in diagnosis and treatment planning has not been thoroughly investigated. This study aimed to investigate the reliability of different generative AI models in classifying the severity of dental crowding.</p><p><strong>Methods: </strong>Two experienced orthodontists categorized the severity of dental crowding in 120 intraoral occlusal images as mild, moderate, or severe (40 images per category). These images were then uploaded to three generative AI models (ChatGPT-4o mini, Microsoft Copilot, and Claude 3.5 Sonnet) and prompted to identify the dental arch and to assess the severity of dental crowding. Response times were recorded, and outputs were compared to orthodontists' assessments. A random image subset was re-analyzed after one week to evaluate model consistency.</p><p><strong>Results: </strong>Claude 3.5 Sonnet successfully classified the severity of dental crowding in 50% of the images, followed by ChatGPT-4o mini (44%), and Copilot (34%). Visual recognition of the dental arches was higher with Claude and ChatGPT-4o mini (99%) compared to Copilot (72%). Response generation was significantly longer for ChatGPT-4o mini than for Claude and Copilot (p < .0001). However, the response times were comparable for both Claude and Copilot (p = .98). Repeated analyses showed improvement in image classification for both ChatGPT-4o mini and Copilot, while Claude 3.5 Sonnet misclassified a significant portion of the images.</p><p><strong>Conclusions: </strong>The performance of ChatGPT-4o mini-, Microsoft Copilot, and Claude 3.5 Sonnet in analyzing the severity of dental crowding often did not match the evaluations made by orthodontists. Further developments in image processing algorithms of commercially available generative AI models are required prior to reliable use for dental crowding classification.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"1558"},"PeriodicalIF":3.1,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12505568/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of different treatment methods on apical closure and treatment success in immature permanent first molars with reversible pulpitis.","authors":"Muhammed Alagoz, Sera Simsek Derelioğlu","doi":"10.1186/s12903-025-06975-3","DOIUrl":"10.1186/s12903-025-06975-3","url":null,"abstract":"<p><strong>Background: </strong>The aim of this study is to evaluate the effects of direct pulp capping and coronal pulpotomy treatments on root development (apexogenesis) and treatment success in immature mandibular first permanent molars with symptoms of reversible pulpitis.</p><p><strong>Methods: </strong>The study included 72 immature mandibular first molars from 60 children aged between 7 and 10 years who applied to the Department of Pediatric Dentistry, Faculty of Dentistry, Ataturk University. Pulpotomy was performed on 34 teeth and direct pulp capping on 38 teeth. In all cases, a single biomaterial (ProRoot MTA) was used, and treatments were completed in a single visit. Clinical and radiographic follow-ups were performed at 3-, 6-, 12-, and 18-month. Root development was assessed using the Moorrees classification.</p><p><strong>Results: </strong>Asymptomatic clinical progress and regular root development were observed in both treatment groups. More than 90% of the treated teeth were successfully followed up to 18 months, and root development progressed consistently according to the Moorrees classification. No statistically significant difference was found between direct pulp capping and pulpotomy in terms of clinical or radiographic success (p >0.05).</p><p><strong>Conclusion: </strong>The data obtained indicate that in immature permanent teeth with reversible pulpitis, both direct pulp capping and pulpotomy treatments can provide similar clinical, radiographic, and physiological outcomes.</p><p><strong>Trial registration: </strong>The study was retrospectively registered in ClinicalTrials.gov ( https://clinicaltrials.gov ) with the registration ID NCT06575062 (2024-08-28).</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"1556"},"PeriodicalIF":3.1,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12505757/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Designed guide plate assisted cortico-cancellous Iliac crest bone grafting for alveolar cleft repair.","authors":"Luxi Weng, Xiaoyan Feng, Yiming Wang, Jiaqi Shao, Xu Huang, Yapei Han, Huiming Wang, Jun Lin","doi":"10.1186/s12903-025-06917-z","DOIUrl":"10.1186/s12903-025-06917-z","url":null,"abstract":"<p><strong>Background: </strong>Alveolar cleft is a common developmental malformation in the maxillofacial region, often occurring with cleft lip or palate. The traditional \"gold standard\" iliac bone grafting implants only cancellous bone in the alveolar cleft region. However, this approach provides insufficient initial mechanical support and is prone to structural collapse and accelerated bone resorption. This study used digital guide plate assisted cortico-cancellous iliac crest bone grafting to repair alveolar clefts, aiming to provide better structural support while reducing bone resorption.</p><p><strong>Method: </strong>This study retrospectively reviewed 39 patients with unilateral alveolar clefts, including 21 cases in the autogenous iliac cancellous bone grafting group (cancellous bone group, mean age 15.33 ± 5.36 years) and 18 cases in the digitally guided autogenous iliac cortical-cancellous composite bone grafting group (composite bone group, mean age 14.17 ± 4.05 years). Preoperative and 6-month postoperative assessments were performed using CBCT for three-dimensional evaluation. The study compared the bone graft volume, new bone fill rate, alveolar width in the grafted area, and bone density between the two groups.</p><p><strong>Result: </strong>Postoperatively, the composite bone group exhibited markedly greater bone graft volume (698.58 ± 197.43 mm³ versus 378.23 ± 176.65 mm³, P < 0.01) and significantly higher new bone fill rate for volume, area, and height relative to the cancellous bone group. The composite bone has a clear advantage in maintaining alveolar ridge width compared to cancellous bone.</p><p><strong>Conclusion: </strong>Compared with the traditional \"gold standard\" grafting, digital guide plate assisted cortico-cancellous iliac crest bone grafting for alveolar cleft repair significantly increased the bone graft volume. The surgical method introduced in this study provides a new option for the precise repair and functional reconstruction of alveolar clefts and has a broad application prospect.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"1567"},"PeriodicalIF":3.1,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12509358/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of CPP-ACP and fluoridated toothpastes in preserving enamel microhardness after erosion and abrasion challenges at different time intervals.","authors":"Laila Al Dehailan, Shahad T Alameer, Fatimah A Alhassan, Razan Fahad Alghamdi, Deena Abdullah Alghamdi, Sukinah Bakr Alabdulmuhsin, Anwar Nabeel Almulhim, Maria Salem Ibrahim, Abdulrahman A Balhaddad","doi":"10.1186/s12903-025-06958-4","DOIUrl":"10.1186/s12903-025-06958-4","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate the impact of fluoridated (1450 ppm F⁻) and CPP-ACP (MI Paste) toothpastes applied after different time points following erosive-abrasive challenge on the perseverance of enamel hardness.  MATERIALS AND METHODS:  Enamel specimens were subjected to an erosive-abrasive model using erosive beverage and toothbrushing simulation. The erosive challenge was achieved by soaking the specimens in Coca-Cola for 2 min. Distilled water was used as a control. Then, the specimens were subjected to brushing using fluoridated and CPP-ACP dentifrices applied via a brushing simulation device. The waiting time between the erosive and abrasive challenges was investigated at four levels (0, 15, 30, 60 min). The Vickers' microhardness values of enamel at baseline and after the erosive-abrasive challenge were analyzed. Paired t-test and ANOVA analyzed the data.</p><p><strong>Results: </strong>Following brushing with distilled water and fluoridated toothpaste, a significant reduction in enamel microhardness was observed at all time points (before vs. after) (P < 0.01). Brushing with CPP-ACP toothpaste achieved significant enamel microhardness reduction at 0 min (P < 0.001) and 30 min (P = 0.007). The erosive challenge and the toothpaste type were significant factors (P < 0.001) in modulating the enamel microhardness but not the waiting time (P = 0.149). There were significant interactions between the erosive challenge and the waiting time (P = 0.005), the toothpaste time and the waiting time (P < 0.001), and there was a significant interaction between the three variables (P = 0.005). CPP-ACP and fluoridated toothpastes resulted in less microhardness reduction compared to brushing using distilled water (P < 0.05), with no difference between CPP-ACP and fluoridated toothpastes.</p><p><strong>Conclusion: </strong>This study found that both fluoridated and CPP-ACP toothpastes are equally effective in protecting enamel, and delaying toothbrushing offers no protective benefits after such exposure. Delaying toothbrushing did not provide any protective benefits for enamel following exposure to the erosive-abrasive model, as fluoridated and CPP-ACP toothpastes were effective in protecting the enamel hardness.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"1553"},"PeriodicalIF":3.1,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12506415/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}