BMC Oral Health最新文献

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Responses of stem cells isolated from human primary teeth to calcium-containing pulp capping materials. 人乳牙分离干细胞对含钙牙髓盖盖材料的反应。
IF 3.1 2区 医学
BMC Oral Health Pub Date : 2025-10-15 DOI: 10.1186/s12903-025-06891-6
Pornchanok Intavongsri, Marcio A da Fonseca, Suphalak Phothichailert, Thanaphum Osathanon, Waleerat Sukarawan
{"title":"Responses of stem cells isolated from human primary teeth to calcium-containing pulp capping materials.","authors":"Pornchanok Intavongsri, Marcio A da Fonseca, Suphalak Phothichailert, Thanaphum Osathanon, Waleerat Sukarawan","doi":"10.1186/s12903-025-06891-6","DOIUrl":"https://doi.org/10.1186/s12903-025-06891-6","url":null,"abstract":"","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"1627"},"PeriodicalIF":3.1,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145298592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Guardians' preferences for pit and fissure sealing services in China: evidence from a discrete choice experiment. 中国监护人对坑和裂隙密封服务的偏好:来自离散选择实验的证据。
IF 3.1 2区 医学
BMC Oral Health Pub Date : 2025-10-15 DOI: 10.1186/s12903-025-07012-z
Zhu Chen, Sixuan Chen, Jiajia Liu, Xinyu Jin, Fangyu Song, Zhengxi Sun, Wang Yao, Wenhua Xu, Lidan Wang
{"title":"Guardians' preferences for pit and fissure sealing services in China: evidence from a discrete choice experiment.","authors":"Zhu Chen, Sixuan Chen, Jiajia Liu, Xinyu Jin, Fangyu Song, Zhengxi Sun, Wang Yao, Wenhua Xu, Lidan Wang","doi":"10.1186/s12903-025-07012-z","DOIUrl":"https://doi.org/10.1186/s12903-025-07012-z","url":null,"abstract":"<p><strong>Background: </strong>Dental caries represents a significant global public health challenge, with particularly high prevalence rates observed among Chinese children. Pit and fissure sealing (PFS) has been established as an effective and cost-efficient preventive intervention, yet service coverage remains suboptimal in China. This study examines guardians' preferences and preference heterogeneity regarding PFS services, with the aim of informing tailored service delivery and policy interventions to enhance PFS uptake.</p><p><strong>Methods: </strong>A discrete choice experiment was conducted through face-to-face interviews with 411 guardians. Four key attributes were included: preventive effectiveness, preventive service time, distance and out-of-pocket (OOP) service cost. Data were analysed using a mixed logit model, subgroup analyses and scenario analyses were performed to examine preference heterogeneity.</p><p><strong>Results: </strong>Preventive effectiveness (41.31%) emerged as the most influential attribute, followed by distance (29.23%), OOP service cost (15.85%) and preventive service time (13.61%). Female guardians and guardians of children with poor oral health status placed greater emphasis on preventive effectiveness and distance. In contrast, male guardians were more focused on OOP service cost. Guardians with higher education levels and guardians of children with better oral health status were more inclined to schedule PFS services during vacations. The scenario analysis revealed that guardians prefer PFS services with general effectiveness but higher accessibility and lower OOP costs, rather than those offering superior effectiveness but compromised accessibility and higher financial burden. Notably, medical insurance coverage boosted service uptake from 18.3% to 71.0%, with guardians willing to pay 127 Chinese yuan more for insured services.</p><p><strong>Conclusion: </strong>These findings highlight substantial preference heterogeneity among guardians and underscore the transformative potential of insurance coverage in enhancing service accessibility. Policymakers should consider incorporating PFS services into medical insurance coverage and optimizing service arrangements to meet the needs of different guardian groups in order to improve the coverage and effectiveness of PFS services.</p><p><strong>Clinical trial number: </strong>not applicable.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"1625"},"PeriodicalIF":3.1,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145298637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluating vision transformers and convolutional neural networks in the context of dental image processing: a systematic review. 评估视觉变压器和卷积神经网络在牙科图像处理的背景下:一个系统的回顾。
IF 3.1 2区 医学
BMC Oral Health Pub Date : 2025-10-15 DOI: 10.1186/s12903-025-07036-5
Turgut Felek, Hümeyra Tercanlı, Rümeysa Şendişçi Gök
{"title":"Evaluating vision transformers and convolutional neural networks in the context of dental image processing: a systematic review.","authors":"Turgut Felek, Hümeyra Tercanlı, Rümeysa Şendişçi Gök","doi":"10.1186/s12903-025-07036-5","DOIUrl":"https://doi.org/10.1186/s12903-025-07036-5","url":null,"abstract":"<p><strong>Background: </strong>The aim of this systematic review is to compare the efficacy of convolutional neural networks (CNN) and Vision Transformers (ViT) in the field of dental imaging, in order to examine in depth the potential, advantages, and limitations of both models in this domain.</p><p><strong>Methods: </strong>The search strings used in the study were \"((\"Vision Transformer\" OR ViT OR \"Transformer architecture\") AND (\"Convolutional Neural Network\" OR CNN OR ConvNet) AND (Dental OR Dentistry OR \"Maxillofacial\" OR \"Oral Radiology\") AND (Image OR Imaging OR Radiograph))\". The search was conducted in January 2025. Two investigators independently evaluated the full texts of all eligible articles and excluded those that did not meet the inclusion/exclusion criteria.</p><p><strong>Results: </strong>Of 2596 articles, 21 met the inclusion criteria. Depending on the task category, of the 21 studies that were reviewed, 14 (66.7%) utilized classification, while 7 (33.3%) utilized segmentation. Panoramic radiography is the most commonly used imaging modality (52.3%) and the ViT-based model was observed to have the highest performance (58%).</p><p><strong>Conclusion: </strong>ViT-based deep learning models tend to exhibit higher performance in many dental image analysis scenarios compared to traditional convolutional neural networks. However, in practice CNN and ViT approaches can be used in a complementary manner.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"1626"},"PeriodicalIF":3.1,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145298628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Measurements of the anatomical structures of the mandibular buccal shelf for the safe insertion of mini-implants: a cone-beam computed tomography retrospective study. 下颌颊架解剖结构的测量用于安全插入微型种植体:锥形束计算机断层回顾性研究。
IF 3.1 2区 医学
BMC Oral Health Pub Date : 2025-10-14 DOI: 10.1186/s12903-025-06980-6
Xing Fan, Lixian Yuan, Zhiwei Wang, Ke Liu, Baiting Luan, Yi Wen, Xin Liu
{"title":"Measurements of the anatomical structures of the mandibular buccal shelf for the safe insertion of mini-implants: a cone-beam computed tomography retrospective study.","authors":"Xing Fan, Lixian Yuan, Zhiwei Wang, Ke Liu, Baiting Luan, Yi Wen, Xin Liu","doi":"10.1186/s12903-025-06980-6","DOIUrl":"https://doi.org/10.1186/s12903-025-06980-6","url":null,"abstract":"<p><strong>Background: </strong>The purpose of this study was to obtain measurements of the anatomical structures of the mandibular buccal shelf (MBS) using cone-beam computed tomography (CBCT) for the safe insertion of orthodontic mini-implants.</p><p><strong>Methods: </strong>The sample consisted of the CBCT records of 100 subjects. The scans were imported into a reconstruction program. Measurements were taken in the coronal plane for three different roots: the mandibular first molar distal root (6D), second molar mesial root (7 M), and second molar distal root (7D). The inferior alveolar nerve canal (IANC) was used as a reference to determine the safe insertion depth. The roots of the mandibular molars were used as a reference to determine the maximum safe insertion angle. Analysis of variance with post hoc analysis and Kruskal-Wallis H test with post hoc analysis were used for data analysis.</p><p><strong>Results: </strong>For the 6Ds, the average bone depth was 20.33 ± 3.6 mm, the average depth above the IANC plane was 9.55 ± 2.97 mm, and the average maximum safe insertion angle was 47.47 ± 18.94°. For the 7Ms, the average bone depth was 22.06 ± 2.85 mm, the average depth above the IANC plane was 10.71 ± 2.67 mm, and the average maximum safe insertion angle was 43.14 ± 15.26°. For the 7Ds, the average bone depth was 23.75 ± 2.86 mm, the average depth above the IANC plane was 12.12 ± 2.61 mm, and the average maximum safe insertion angle was 28.39 ± 10.96°. From anterior to posterior, the buccal bone depth and depth above the IANC gradually increased (P < 0.01), there was no significant difference in the maximum safe insertion angle between the 6Ds and 7Ms (P = 1.000), but the significant difference was observed between 6Ds and 7Ds, 7Ms and 7Ds (P < 0.000).</p><p><strong>Conclusions: </strong>The optimal implantation site for mini-implant in the MBS is at the buccal site corresponding to the second molar. A maximum insertion length of ≤ 12 mm and a maximum insertion angle which respects the long axial of the tooth root of ≤ 28° are recommended for the safe insertion of mini-implants in the MBS.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"1612"},"PeriodicalIF":3.1,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Parotid intranodal metastasis of Merkel cell carcinoma: a rare case report. 梅克尔细胞癌腮腺结内转移1例。
IF 3.1 2区 医学
BMC Oral Health Pub Date : 2025-10-14 DOI: 10.1186/s12903-025-06921-3
Tong Gao, Dengshun Wang, Hongwei Yu, Yu'e Wang, Haibin Lu
{"title":"Parotid intranodal metastasis of Merkel cell carcinoma: a rare case report.","authors":"Tong Gao, Dengshun Wang, Hongwei Yu, Yu'e Wang, Haibin Lu","doi":"10.1186/s12903-025-06921-3","DOIUrl":"https://doi.org/10.1186/s12903-025-06921-3","url":null,"abstract":"<p><strong>Background: </strong>Merkel cell carcinoma is a rare, highly aggressive neuroendocrine skin tumor with a low incidence rate, typically arising on sun-exposed areas. This case report aims to describe an unusual metastatic pattern involving the parotid gland lymph nodes and to discuss treatment strategies, thereby raising awareness of the disease and providing clinical insights.</p><p><strong>Case presentation: </strong>A 63-year-old female presented with a mass in the left parotid gland region persisting for two months. Clinical examination revealed a firm, well-defined, mobile mass measuring approximately 2.0 × 2.0 cm at the lower posterior pole of the left parotid gland, located behind the left ear. The mass was non-tender, without spontaneous pain, ulceration, redness, or local temperature elevation. The patient had previously undergone excision of an earlobe mass at another hospital without pathological examination. CT imaging demonstrated a space-occupying lesion in the left parotid gland. Under general anesthesia, the superficial lobe of the left parotid gland and the mass were excised. Postoperative paraffin histopathology and immunohistochemistry confirmed Merkel cell carcinoma with lymph node metastasis in the left parotid gland. A PET-CT scan performed 10 days postoperatively revealed no definite primary lesion and no distant metastasis. At present, the patient's systemic and local conditions remain stable.</p><p><strong>Conclusion: </strong>The diagnosis of Merkel cell carcinoma is challenging due to its rarity and early metastatic tendency, often resulting in misdiagnosis or missed diagnosis. Although imaging studies assist in detecting metastatic lesions, parotid gland lymph node metastases may present with nonspecific features, leading to diagnostic confusion with other parotid diseases. Histopathological examination remains essential, and the complex immunohistochemical profile necessitates the use of multiple markers for accurate diagnosis.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"1607"},"PeriodicalIF":3.1,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Is stigma correlated with oral health-related quality of life in prosthodontic patients? a cross-sectional study. 病耻感与修复患者口腔健康相关生活质量相关吗?横断面研究。
IF 3.1 2区 医学
BMC Oral Health Pub Date : 2025-10-14 DOI: 10.1186/s12903-025-06935-x
Zhe-Bin Yan, Rui Zhu, Xiao Yi, Ya-Peng Pei, Mei-Jun Zhan, Jia-Le Zhu, Hai-Yang Yu
{"title":"Is stigma correlated with oral health-related quality of life in prosthodontic patients? a cross-sectional study.","authors":"Zhe-Bin Yan, Rui Zhu, Xiao Yi, Ya-Peng Pei, Mei-Jun Zhan, Jia-Le Zhu, Hai-Yang Yu","doi":"10.1186/s12903-025-06935-x","DOIUrl":"https://doi.org/10.1186/s12903-025-06935-x","url":null,"abstract":"<p><strong>Background: </strong>The impact of psychological factors on oral health-related quality of life (OHRQoL) in prosthodontic patients should not be ignored. It is unclear about the extent to which stigma affects OHRQoL. The purpose of this study was to examine the relationship between stigma and OHRQoL in prosthodontic patients.</p><p><strong>Methods: </strong>A total of 139 patients were included. Stigma Scale for Chronic Illness (SSCI) and its subscales (enacted stigma (ES) and internalized stigma (IS)) were used to measure stigma. OHRQoL was evaluated utilizing 5-item Oral Health Impact Profile (OHIP-5), Psychosocial Impact of Dental Aesthetics Questionnaire (PIDAQ) and its subscales (Social Impact (SI), Psychological Impact (PI), Aesthetic Concern (AC) and Dental Self-Confidence (DSC)). The association between SSCI and OHIP/PIDAQ was detected employing Spearman correlation coefficients and multiple linear regression analysis.</p><p><strong>Results: </strong>Univariate analysis revealed that SSCI-total, ES, and IS scores were positively correlated with OHIP-5 (r<sub>s</sub>=0.350, 0.362, 0.279, P < 0.001); the first three were also positively correlated with PIDAQ-total (r<sub>s</sub>=0.625, 0.589, 0.566, P < 0.001), SI (r<sub>s</sub>=0.605, 0.548, 0.572, P < 0.001), PI (r<sub>s</sub>=0.649, 0.634, 0.551, P < 0.001), and AC (r<sub>s</sub>=0.580, 0.536, 0.534, P < 0.001), except for PIDAQ-DSC (P > 0.05). Upon controlling for confounders in multivariate analysis, SSCI-total, ES and IS maintained a strong positive correlation with OHIP-5 (B = 0.354, 0.427, 0.866, P < 0.001), PIDAQ-total (B = 2.329, 3.044, 5.138, P < 0.001), SI (B = 1.057, 1.458, 2.155, P < 0.001), PI (B = 0.813, 1.020, 1.893, P < 0.001), and AC (B = 0.423, 0.561, 0.916, P < 0.001), with the exception of PIDAQ-DSC (P > 0.05).</p><p><strong>Conclusions: </strong>Stigma was negatively correlated with OHRQoL in prosthodontic patients. Higher stigma may impair OHRQoL. Interventions to alleviate the stigma in such patients is recommended to improve OHRQoL.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"1614"},"PeriodicalIF":3.1,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Immediate implant placement for single failed maxillary anterior teeth after trauma: a case-series study. 创伤后单颗上颌前牙失败的即刻种植:一项病例系列研究。
IF 3.1 2区 医学
BMC Oral Health Pub Date : 2025-10-14 DOI: 10.1186/s12903-025-06878-3
Zinan Yang, Xiao Chen, Jingyun Chen, Yinyin Li, Qian Liang
{"title":"Immediate implant placement for single failed maxillary anterior teeth after trauma: a case-series study.","authors":"Zinan Yang, Xiao Chen, Jingyun Chen, Yinyin Li, Qian Liang","doi":"10.1186/s12903-025-06878-3","DOIUrl":"https://doi.org/10.1186/s12903-025-06878-3","url":null,"abstract":"<p><strong>Background: </strong>The purpose of this retrospective evaluation was to assess the treatment outcomes following immediate implant placement for single failed maxillary anterior teeth after trauma. Moreover, both objective and subjective esthetic assessments were conducted.</p><p><strong>Methods: </strong>Immediate implant placement was performed on thirty-two patients who had a single untreatable, traumatic tooth in the anterior maxilla. At the one-year mark following the final restoration, marginal bone changes, pink/white esthetic scores (PES/WES), and patient satisfaction data were collected. Factors such as age, sex, gingival biotype, thickness of the buccal bone wall, and size of the jumping gap were considered potential influences on the clinical results, with their relationships being examined.</p><p><strong>Results: </strong>The implant survival rate was 100% after one year of final restoration., with marginal bone changes measuring - 0.17 ± 0.31 mm mesially and - 0.22 ± 0.28 mm distally. Additionally, the PES averaged 10.7, whereas the WES averaged 7.3. On the patient side, esthetic satisfaction scored an average of 8.89 on the visual analog scale. Interestingly, no significant correlations were found between potential factors and esthetic scores or patient satisfaction.</p><p><strong>Conclusions: </strong>In the initial year following the final restoration, immediate implant placement for single failed maxillary anterior teeth post-trauma demonstrated promising clinical outcomes both objectively and subjectively.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"1605"},"PeriodicalIF":3.1,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development and validation of artificial intelligence models for automated periodontitis staging and grading using panoramic radiographs. 开发和验证人工智能模型用于自动牙周炎分期和分级使用全景x线片。
IF 3.1 2区 医学
BMC Oral Health Pub Date : 2025-10-14 DOI: 10.1186/s12903-025-07025-8
Khiem Quang Do, Truc Thanh Thai, Viet Quoc Lam, Thuy Thu Nguyen
{"title":"Development and validation of artificial intelligence models for automated periodontitis staging and grading using panoramic radiographs.","authors":"Khiem Quang Do, Truc Thanh Thai, Viet Quoc Lam, Thuy Thu Nguyen","doi":"10.1186/s12903-025-07025-8","DOIUrl":"https://doi.org/10.1186/s12903-025-07025-8","url":null,"abstract":"<p><strong>Background: </strong>Periodontal diseases are common chronic conditions that can lead to tooth loss and systemic complications if not diagnosed and treated promptly. The 2017 classification by the American Academy of Periodontology highlights the need for effective, accurate diagnostic tools. This study aimed to develop and validate an AI-driven system for automated staging and grading periodontitis from panoramic radiographs using the YOLOv8 architecture.</p><p><strong>Methods: </strong>A total of five hundred panoramic radiographs from patients diagnosed with periodontitis were included. Radiographs were labeled and split into training (75%), validation (15%), and testing (10%) sets. Three specialized YOLOv8-based models were trained to segment the alveolar bone level, the cemento-enamel junction (CEJ), and tooth axes. Image augmentations were applied to enhance model robustness. The resulting measurements of radiographic bone loss were combined with patient information (age, smoking status, diabetes) to identify periodontitis stage and grade following the 2017 guidelines.</p><p><strong>Results: </strong>The bone level and CEJ detection models achieved high precision (0.95-0.97) and recall (0.94-0.96), reflecting strong segmentation performance. The tooth detection model achieved a precision of approximately 0.82 and a recall of 0.81. Integrating all three models enabled automated determination of periodontal stage (I-IV) and grade (A-C), with an interactive interface allowing clinicians to review and adjust outputs if necessary.</p><p><strong>Conclusion: </strong>The proposed YOLOv8-based framework accurately detects key periodontal landmarks and automates disease staging and grading. Future work should expand the dataset, refine the tooth detection model, and validate the system in clinical settings to support large-scale periodontal screening and improved patient care.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"1623"},"PeriodicalIF":3.1,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Silymarin-functionalized nanohydroxyapatite-chitosan nanocomposite: a promising biomaterial for oral health applications. 水飞蓟素功能化纳米羟基磷灰石-壳聚糖纳米复合材料:一种具有口腔健康应用前景的生物材料。
IF 3.1 2区 医学
BMC Oral Health Pub Date : 2025-10-14 DOI: 10.1186/s12903-025-06784-8
Aravind Kumar Subramanian, Gautham Sivamurthy, Karen Sarkisovich Karapetyan, Ammar Al-Farga, Rashad Saleh, Mohammad Ali Shariati
{"title":"Silymarin-functionalized nanohydroxyapatite-chitosan nanocomposite: a promising biomaterial for oral health applications.","authors":"Aravind Kumar Subramanian, Gautham Sivamurthy, Karen Sarkisovich Karapetyan, Ammar Al-Farga, Rashad Saleh, Mohammad Ali Shariati","doi":"10.1186/s12903-025-06784-8","DOIUrl":"https://doi.org/10.1186/s12903-025-06784-8","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Background: &lt;/strong&gt;Nanohydroxyapatite (nHAP) is widely recognized for its potential biomedical applications, particularly in bone regeneration and periodontal therapy. Green synthesis methods, which are eco-friendly and non-toxic, have gained attention for the production of nanocomposites. Silymarin, a bioactive compound, can serve as both a reducing and stabilizing agent in such synthetic processes. In this study, we aimed to develop a nanohydroxyapatite nanocomposite using silymarin (SL) and chitosan (CH) and evaluate its antioxidant, antibacterial, and anti-inflammatory properties for oral health applications.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;Nanohydroxyapatite nanocomposites were synthesized using a green synthesis technique, with silymarin acting as both reducing and stabilizing agent. Chitosan was incorporated to form the polymer-based nanocomposites. The synthesized materials (silymarin-chitosan, silymarin-nHAP, and nanocomposite) were characterized by Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), and Fourier Transform Infrared Spectroscopy (FTIR) to assess their morphology and functional groups. Antioxidant activity was evaluated using the DPPH assay, and the antibacterial activity was tested against common oral pathogens. Biocompatibility was assessed using human gingival fibroblast (HGF) cells and cell viability was measured via live/dead cell assays using fluorescence imaging and in vitro migration assays. Wettability analysis was performed using a contact angle measurement technique to evaluate the surface hydrophilicity of the nanocomposite, a crucial factor for biointegration and tissue adhesion. Additionally, the anti-inflammatory potential was examined using the human red blood cell (HRBC) membrane stabilization assay, where the nanocomposite was evaluated for its ability to inhibit heat-induced hemolysis.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;The characterization revealed the successful formation of nanoHA nanocomposites with distinct morphological features. Antioxidant assays indicated significant free radical scavenging activity, whereas antibacterial testing demonstrated effective inhibition of oral pathogens, including Streptococcus mutans and Porphyromonas gingivalis. Wettability analysis revealed a favorable contact angle, indicating enhanced surface hydrophilicity, which is beneficial for cell attachment and biointegration. Biocompatibility studies revealed that the nanocomposites exhibited minimal cytotoxicity and enhanced cell viability. Migration assays revealed favorable activity in promoting fibroblast migration, suggesting its potential for tissue regeneration. Furthermore, the HRBC membrane stabilization assay confirmed the anti-inflammatory potential of the nanocomposite, indicating its ability to protect erythrocytes against heat-induced hemolysis.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;The silymarin-chitosan-nanohydroxyapatite nanocomposite synthesized using green methods demonstra","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"1617"},"PeriodicalIF":3.1,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The key role of magnetic resonance sialography in the differential diagnosis between sialolith and phlebolith of the floor of the mouth. 磁共振唾液造影在鉴别诊断口腔底的唾液石和静脉石中的关键作用。
IF 3.1 2区 医学
BMC Oral Health Pub Date : 2025-10-14 DOI: 10.1186/s12903-025-06795-5
Alvaro Sánchez Barrueco, Gonzalo Díaz Tapia, Félix Guerra Gutiérrez, Elena Salvador Álvarez, José Miguel Villacampa Aubá
{"title":"The key role of magnetic resonance sialography in the differential diagnosis between sialolith and phlebolith of the floor of the mouth.","authors":"Alvaro Sánchez Barrueco, Gonzalo Díaz Tapia, Félix Guerra Gutiérrez, Elena Salvador Álvarez, José Miguel Villacampa Aubá","doi":"10.1186/s12903-025-06795-5","DOIUrl":"https://doi.org/10.1186/s12903-025-06795-5","url":null,"abstract":"<p><strong>Background: </strong>The confusion between a phlebolith and a sialolith in the floor of the mouth can pose serious surgical risks, making it essential to define appropriate complementary imaging studies.</p><p><strong>Case presentation: </strong>We present the case of a patient with right submandibular sialolithiasis suspected by computed tomography. A previous attempt at extraction in another centre, under local anesthesia, was interrupted due to profuse bleeding, preventing the localization and removal of the calculi. Due to our pre-surgical study protocol using magnetic resonance sialography (MR-Si), a low-flow vascular malformation with internal phleboliths was revealed, without involvement of the salivary duct. As a result, the planned sialendoscopy was cancelled, and a follow-up approach was chosen given the asymptomatic nature of the condition.</p><p><strong>Conclusions: </strong>This case underscores the importance of standardizing radiological studies for salivary gland and floor-of-mouth pathology, highlighting MR-Si for its high sensitivity and specificity. Therefore, MR-Si enables more precise surgical planning and helps prevent unnecessary intraoperative complications.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"1615"},"PeriodicalIF":3.1,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145290918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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