BMC Oral Health最新文献

{"title":"Comparison of the antibacterial properties of Resin cements with and without the addition of nanoparticles: a systematic review.","authors":"Ravinder Saini, Sunil Kumar Vaddamanu, Masroor Ahmed Kanji, Syed Altafuddin Quadri, Saeed Awod Bin Hassan, Sukumaran Anil, Deepti Shrivastava, Kumar Chandan Srivastava","doi":"10.1186/s12903-024-05013-y","DOIUrl":"https://doi.org/10.1186/s12903-024-05013-y","url":null,"abstract":"<p><p>Resin cement exhibits numerous therapeutic advantages over conventional luting materials. However, the effectiveness of the antibacterial properties of resin cement remains unclear. Nanotechnology provides a viable option, whereby the integration of nanoparticles (NPs) can potentially augment the antibacterial effectiveness of resin cement. The objective of this study was to conduct a comprehensive literature review to assess resin cement's antibacterial effectiveness by incorporating nanoparticles. An extensive search of PubMed and Scopus databases up to September 12, 2023, was conducted to identify relevant scholarly articles that examined and evaluated resin cement's antibacterial effectiveness with and without the incorporation of nanoparticles (NPs). This systematic review adhered to the PRISMA guidelines for reporting the results. The search retrieved seven eligible studies. Studies indicated that resin cement with NPs significantly reduced the colony forming unit (CFU) counts compared to resin cement without NPs. Furthermore, resin cement, in addition to NPs, significantly reduced the bacterial metabolic activity compared to the control group. The use of nanoparticles (NPs) in resin cement has been shown to enhance its antibacterial properties, possibly mitigating the occurrence of secondary caries. Future clinical trials are required to validate the beneficial effects of NPs in conjunction with resin cement in the prevention of secondary caries.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"24 1","pages":"1426"},"PeriodicalIF":2.6,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of interleukin-20 on inflammatory stress and periodontal tissue destruction in patients with metabolic syndrome and periodontitis.","authors":"Reyhan Senkal, Hatice Yemenoglu, Oguz Kose, Sibel Mataraci Karakas, Adnan Yilmaz, Kerimali Akyildiz, Melek Beder, Semih Alperen Bostan","doi":"10.1186/s12903-024-05224-3","DOIUrl":"https://doi.org/10.1186/s12903-024-05224-3","url":null,"abstract":"<p><strong>Background: </strong>There is an increasing occurrence of periodontitis and metabolic syndrome (MetS), which is resulting in a decline in the overall quality of life. Both disorders can occur together since they are both linked to insulin resistance and systemic inflammation. However, evidence for a role of interleukin (IL)-20 in this comorbidity is very limited. This cross-sectional study aimed to comprehensively investigate, for the first time, the levels of RANKL/OPG, MMP-8 and OSI as well as the role of IL-20 in patients with MetS and periodontitis.</p><p><strong>Methods: </strong>The study included a total of 80 individuals, divided into four groups: 20 individuals who were healthy both systemically and periodontally, 20 individuals who were systemically healthy but had periodontitis, 20 individuals who had MetS but were periodontally healthy, and 20 individuals who had both MetS and periodontitis. Periodontal clinical parameters (plaque index, gingival index, bleeding on probing, clinical attachment loss, probing pocket depth) were evaluated. Gingival crevicular fluid (GGF) and serum samples were collected and used for biochemical assays. Enzyme-linked immunosorbent assay was used to determine the levels of IL-20, receptor activator of nuclear factor kappa B ligand (RANKL)/osteoprotegerin (OPG), matrix metalloproteinase-8 (MMP-8) and oxidative stress index (OSI).</p><p><strong>Results: </strong>IL-20 levels measured in serum and GCF were statistically significantly highest in patients with MetS and periodontitis (p = 0.001). Significant positive correlation was observed between serum and GCF IL-20 values and periodontal parameters (p < 0.05). There was a positive correlation between RANKL and RANKL/OPG levels and IL-20 and clinical parameters (p < 0.05). OSI values were found to be increased in the presence of both periodontitis and MetS (p = 0.001) and were positively correlated with serum and GCF IL-20 (p < 0.05).</p><p><strong>Conclusions: </strong>These data from the study suggest a correlation between IL-20 and both MetS and periodontitis. IL-20 may potentially worsen the condition of periodontal tissue by increasing both the oxidative stress levels, periodontal collagen degredation and the ratio of RANKL to OPG.</p><p><strong>Trial registration: </strong>This study was registered on ClinicTrials.gov (NCT06092853), 2023-10-10, retrospectively registered.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"24 1","pages":"1423"},"PeriodicalIF":2.6,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salivary microbiome variations in type 2 diabetes mellitus patients with different stages of periodontitis.","authors":"Min Gu, Junyi Ge, Qianjia Pan, Nan Hu, Fei Hua","doi":"10.1186/s12903-024-05135-3","DOIUrl":"https://doi.org/10.1186/s12903-024-05135-3","url":null,"abstract":"<p><strong>Background: </strong>To investigate the difference of salivary microbiome composition in type 2 diabetes mellitus with different stages of periodontitis.</p><p><strong>Methods: </strong>105 patients diagnosed with T2DM were divided into four groups according to the Stage of periodontitis.The 16 S rDNA hypervariable regions V3-V4 were amplified by polymerase chain reaction (PCR) after saliva DNA was extracted. The library was constructed and Illumina sequencing was performed. The sequencing results were compared with the silva 138 database, and the salivary microbiome of the four groups of patients was studied by species composition analysis, species difference analysis, principal coordinate analysis, and correlation analysis.</p><p><strong>Results: </strong>There were significant differences in alpha diversity of salivary microbiome among the four groups, but no difference in Beta diversity. At the phylum level, there was one differential bacteria-Firmicutes, while at the genus level, there were 16 differential bacteria. Spearman's correlation analysis identified significant correlations between periodontal health indices (PLI, SBI, AL and PD) and the abundances of over 13 genera, including Streptococcus, Gemella and Capnocytophaga, etc. Additionally, the duration of diabetes exhibited a positive correlation with AL and PD.</p><p><strong>Conclusions: </strong>There are differences in salivary microbial composition in patients with type 2 diabetes mellitus with different stages of periodontitis. There is a correlation between some saliva microbiota and periodontal inflammation.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"24 1","pages":"1424"},"PeriodicalIF":2.6,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors associated with the survival of oral cavity cancer patients: a single institution experience from Karachi, Pakistan.","authors":"Uzma Shamsi, Muhammad Ali Akbar Khan, Mohammad Shahzaib Qadir, Shaikh Saif Ur Rehman, Iqbal Azam, Romana Idress","doi":"10.1186/s12903-024-04920-4","DOIUrl":"https://doi.org/10.1186/s12903-024-04920-4","url":null,"abstract":"<p><strong>Background: </strong>Oral cavity cancer (OCC) represents a significant public health burden in South Asia, ranking as the most prevalent cancer affecting males in Pakistan and contributing considerably to morbidity and mortality. The objective of this study was to evaluate the survival rates in patients diagnosed with OCC in the Pakistani population and identify the factors influencing survival.</p><p><strong>Methods: </strong>This was a retrospective cohort study conducted at the Aga Khan University Hospital Karachi, Pakistan. A total of 2238 cases diagnosed with OCC from May 2011 through December 2020, who met the inclusion criteria, were included. Survival analyses at 1-, 3- and 5-year intervals were performed using the Kaplan-Meier method. Adjusted hazard ratios (HR) and their 95% confidence intervals (CIs) were estimated using a Cox regression model to identify the factors significantly influencing survival.</p><p><strong>Results: </strong>Among all cases, 54.0% were diagnosed in individuals 50 years of age and below and 73.0% were tobacco users. Overall survival, 1-year, 3-year, and 5-year survival rates were 54.9%, 88.9%, 81.6%, and 75.9% respectively. After adjustment for all the significant confounding and prognostic factors, age was an important factor showing that adults > 50 years had poorer survival compared to ≤ 50 years (HR = 1.43, 95% CI = 1.11-1.83). Poorly differentiated grade of OCC compared to well-differentiated grade (HR = 2.49, 95% CI = 1.61-3.84) and stage IV compared to earlier stages (HR = 3.56, 95% CI = 2.13-5.94) were also associated with poor survival.</p><p><strong>Conclusion: </strong>Notably, there was a high occurrence of late stage (III and IV) OCC in individuals aged ≤ 50 years, with the buccal mucosa being the most common site involved. Moreover, a large proportion of our patients had a history of tobacco use. Patients with advanced-stage disease and poorly differentiated grade showed poor survival. Therefore, public awareness about tobacco control for OCC prevention and earlier diagnosis of OCC is urgently needed in our Pakistani population.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"24 1","pages":"1427"},"PeriodicalIF":2.6,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reliability of the digital functionally generated path technique for assessing occlusal interferences and adjusting CAD-CAM zirconia crowns: an in vivo study.","authors":"Sherine Anwar Saad, Yousreya Atteya Shalaby, Amir Shoukry Azer","doi":"10.1186/s12903-024-05202-9","DOIUrl":"https://doi.org/10.1186/s12903-024-05202-9","url":null,"abstract":"<p><strong>Background: </strong>Designing the occlusal surface of a prosthesis to ensure optimal eccentric occlusion is challenging without precisely replicating the patient's mandibular movements. During the CAD-CAM prosthesis delivery process, clinicians often need to make adjustments to the prosthesis to avoid occlusal interferences that may occur during lateral excursions. Recently, there have been developments in the field of mandibular motion tracking using optical devices. These approaches seek to incorporate an individual's functional movement into the research field of occlusal morphology.</p><p><strong>Aim: </strong>This study aimed to assess the accuracy of digitally replicating mandibular movements to identify and correct occlusal interferences in monolithic CAD-CAM zirconia crowns.</p><p><strong>Methods: </strong>An intraoral scanner (IOS) was used to capture complete arch maxillary and mandibular teeth and record buccal and lateral interocclusal records of maxillary first premolar abutment teeth of thirteen participants. For each patient, two monolithic zirconia crowns were fabricated following the standard digital workflow. The crowns were categorized based on the virtual method used for adjusting occlusal interferences into two groups: Group I, where occlusal interferences in CAD-CAM zirconia crowns were adjusted using buccal interocclusal records, and Group II, where adjustments were made using both buccal and lateral interocclusal records. After crown fabrication following the manufacturer's instructions, occlusion was analyzed using an electronic pressure analyzer. The mean, standard deviation and median values of the recorded data were measured. Paired t test and Wilcoxon Sign Rank test were executed for analyzing differences between groups (p value ≤ 0.05).</p><p><strong>Results: </strong>Group I recorded higher maximum pressure at lateral mandibular movement with mean ± standard deviation value of 26.00 ± 4.95% than Group II with 20.62 ± 3.38%. Regarding pressure recorded at maximum intercuspation (MI) Group I showed higher results; 8.08 ± 1.50% compared to Group II with 7.23 ± 1.59% mean ± standard deviation value. The average value of crown volume for Group I was (160.36 ± 15.94) mm³, while for Group II was (157.63 ± 14.45) mm³.</p><p><strong>Conclusions: </strong>The digital functionally generated path technique allows for identifying occlusal interferences and modifying CAD-CAM zirconia crown designs.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"24 1","pages":"1425"},"PeriodicalIF":2.6,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142692308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Birth order, daycare attendance, and childhood socioeconomic status in relation to gingivitis: a cross-sectional study in Turkish young adults.","authors":"Zeynep Tastan Eroglu, Dilek Ozkan Sen, Osman Babayigit, Kaan Yildiz","doi":"10.1186/s12903-024-05215-4","DOIUrl":"https://doi.org/10.1186/s12903-024-05215-4","url":null,"abstract":"<p><strong>Aim: </strong>Birth order, sibship size, breastfeeding, daycare attendance, and delivery method are known to influence the development of gut microbiota and the immune system, affecting the risk of many infectious diseases. This study aims to explore the impact of these factors, along with socioeconomic background and familial factors, on gingivitis and oral hygiene practices in early adulthood among the Turkish population.</p><p><strong>Materials and methods: </strong>Patients were diagnosed using periodontal clinical and radiographic assessments, adhering to the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions. The study included 18-year-old individuals diagnosed with either generalized gingivitis or clinical gingival health. Data collected included sociodemographic factors, birth order, sibship size, delivery methods, breastfeeding duration, daycare attendance, and oral hygiene behaviors. Binary logistic regression was employed to analyze the associations between these factors and the presence of generalized gingivitis.</p><p><strong>Results: </strong>A total of 178 individuals were evaluated, including 124 with generalized gingivitis and 54 with clinical gingival health. Daycare attendance was significantly associated with gingival health. Among those with gingival health, 55.6% had attended daycare, whereas only 29% of participants with generalized gingivitis had attended daycare. Conversely, 71% of those with generalized gingivitis did not attend daycare, compared to 44.4% in the gingival health group (p = 0.001). Univariate logistic regression identified a significant association between daycare attendance and a reduced risk of generalized gingivitis (Odds Ratio [OR] = 0.327, p = 0.001). Later birth order was associated with a reduced risk of gingivitis (OR = 0.470, p = 0.037). Sibship size, delivery methods, and breastfeeding duration showed no clear associations with gingivitis (p > 0.05).</p><p><strong>Conclusions: </strong>The findings indicate a potential association between oral health status in adulthood and early childhood exposures, such as daycare attendance and birth order. Further research is necessary to confirm these associations and elucidate the mechanisms involved, even though these factors may contribute to gingival health in early adulthood. These insights may enhance our understanding of how early-life exposures relate to oral health outcomes and suggest that considering such factors could be beneficial in future oral health management strategies.</p><p><strong>Trial registration: </strong>Trial registration is also available at clinicaltrail.gov. Clinical Trials-ID: NCT06240247. Registration Date: 22.01.2024.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"24 1","pages":"1420"},"PeriodicalIF":2.6,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142685932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of serum levels in children with delayed eruption.","authors":"Meryem Sahin, Ismet Rezani Toptanci","doi":"10.1186/s12903-024-05210-9","DOIUrl":"https://doi.org/10.1186/s12903-024-05210-9","url":null,"abstract":"<p><strong>Aim: </strong>This study aimed to assess levels of 25-hydroxy vitamin D (25(OH)D₃), calcium (Ca⁺²), phosphorus (P), and parathyroid hormone (PTH) and to determine delayed tooth eruption by examining their correlations.</p><p><strong>Material and method: </strong>The study included 101 paediatric patients, aged 6-14 years, who visited the Dicle University Paediatric Dentistry Clinic, had no systemic diseases, and had not received medication in the past three months. Among them, 60 patients exhibited delayed eruption of their permanent central and first molar teeth, while 41 did not. Serum levels of 25(OH)D₃, Ca⁺², P, and PTH were measured. Statistical analyses were performed using IBM SPSS software, with statistical significance set at p < 0.05.</p><p><strong>Results: </strong>Serum levels of 25(OH)D₃ and Ca⁺² were significantly lower in the patient group, whereas PTH levels were significantly lower in the control group (p < 0.05). However, serum P levels did not differ significantly between the groups (p > 0.05).</p><p><strong>Conclusions: </strong>Serum parameters play a significant role in tooth eruption. The significance of vitamin D has increased because of its physiological effects and involvement in metabolic pathways, highlighting the need to examine Ca⁺², P, and PTH levels, which contribute to its regulation. Low levels of vitamin D and Ca⁺², along with elevated PTH levels, have been identified as potential factors contributing to delayed tooth eruption, whereas P levels do not appear to have a significant impact. In view of the ongoing growth and development in this patient group, regular monitoring of vitamin D, Ca⁺², and PTH levels, along with timely interventions, is essential.</p><p><strong>Trial registration: </strong>TCTR identification number: TCTR20240729001; registered on 29 July 2024. The trial was registered retrospectively.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"24 1","pages":"1418"},"PeriodicalIF":2.6,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142685943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of transglutaminase 2 in promoting biglycan synthesis in idiopathic gingival fibromatosis.","authors":"Yurika Ninomiya, Shinji Matsuda, Shigeki Suzuki, Shizu Hirata-Tsuchiya, Tomoya Ueda, Fuminori Nakashima, Keisuke Yasuda, Shogo Shimada, Takumi Memida, Tetsuya Yoshimoto, Satoru Yamada, Kazuhisa Ouhara, Noriyoshi Mizuno","doi":"10.1186/s12903-024-05211-8","DOIUrl":"10.1186/s12903-024-05211-8","url":null,"abstract":"<p><strong>Background: </strong>This study aimed to elucidate the pathogenesis of idiopathic gingival fibromatosis (IGF).</p><p><strong>Methods: </strong>Human gingival fibroblasts (hGFs) were isolated from patients with IGF and periodontitis. Differential gene expression in the hGFs was analyzed using RNA sequencing. Extracellular matrix-related gene expression in the hGFs was analyzed. The effect of specific protein (SP)1 inhibitor or recombinant human transglutaminase 2 (rh-TGM2) on biglycan (BGN) expression in hGFs was also determined.</p><p><strong>Results: </strong>RNA sequencing showed that TGM2 expression was downregulated and BGN mRNA expression was upregulated in patients with IGF relative to periodontitis. rh-TGM2 stimulation of hGFs in patients with IGF significantly reduced BGN expression. SP1 inhibitors downregulated BGN expression in the hGFs.</p><p><strong>Conclusion: </strong>BGN upregulation via SP1 causes TGM2 downregulation in gingival fibroblasts in IGF.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"24 1","pages":"1422"},"PeriodicalIF":2.6,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142685947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of different boron contents within boron-doped hydroxyapatite-chitosan nano-composite on the microhardness of demineralized enamel.","authors":"Bahare Asgartooran, Amirhossein Bahadori, Zahra Khamverdi, Erfan Ayubi, Abbas Farmany","doi":"10.1186/s12903-024-05194-6","DOIUrl":"https://doi.org/10.1186/s12903-024-05194-6","url":null,"abstract":"<p><strong>Objective: </strong>Dental caries is a prevalent chronic disease globally, arising from an imbalance between tooth remineralization and demineralization. Early detection of lesions is crucial, with non-invasive methods preferred in the initial stages of caries for enamel remineralization and demineralization prevention. This study aimed to assess the impact of varying contents of boron-doped hydroxyapatite-chitosan nano-composite on the microhardness of demineralized enamel.</p><p><strong>Materials and methods: </strong>Boron-doped hydroxyapatite-chitosan nano-composites at concentrations of 0%, 5%, 10%, and 15% were prepared using the sol-gel method. Thirty intact human premolars were collected to evaluate tooth remineralization. The teeth were divided into buccal and lingual halves, subjected to pH cycling for 14 days to demineralize the enamel surface. Subsequently, the specimens were randomly divided into four groups (n = 15) based on the application of remineralizing materials: Group 1 - Hydroxyapatite-chitosan nano-composite (HApC); Group 2 - Boron-doped hydroxyapatite-chitosan nano-composite (B@HApC) with 5% boron concentration; Group 3 - Boron-doped hydroxyapatite-chitosan nano-composite (B@HApC) with 10% boron concentration; and Group 4 - Boron-doped hydroxyapatite-chitosan nano-composite (B@HApC) with 15% boron concentration. Microhardness was measured at three points using a Vickers hardness test (300 g force for 10 s).</p><p><strong>Results: </strong>The study revealed a significant difference between the nano-composite groups containing 5%, 10%, and 15% boron compared to the control group (p < 0.001). The highest microhardness was observed in the 15% boron group (49.42), with the control group showing the lowest microhardness (20.15). Pairwise comparisons of microhardness indicated a significant difference between Group 4 and the control group (p < 0.05). However, no significant differences were found between Groups 2 and 3, Groups 2 and 4, and Groups 3 and 4 (p > 0.05). Scanning electron microscope (SEM) results supported the microhardness findings, showing a mineral layer covering the porous structure resulting from enamel surface demineralization in the intervention groups, highlighting the effectiveness of boron in enhancing the microhardness of demineralized enamel.</p><p><strong>Conclusion: </strong>The study demonstrated that a content of 15% boron in B@HApC and higher led to increased microhardness of demineralized enamel. Additionally, content of 5% and 10% boron in the nano-composite also improved microhardness, although not to a statistically significant extent.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"24 1","pages":"1419"},"PeriodicalIF":2.6,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142685934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wnt signaling aberrant activation drives ameloblastoma invasion and recurrence: bioinformatics and in vitro insights.","authors":"Yemei Qian, Hongrong Zhang, Jingyi Li, Liangchong Huang, Yunfa Qin, Jian Zhang, Weihong Wang","doi":"10.1186/s12903-024-05003-0","DOIUrl":"10.1186/s12903-024-05003-0","url":null,"abstract":"<p><strong>Objective: </strong>This study aims to explore the regulatory mechanisms of Wnt signaling in the invasion and recurrence of ameloblastoma (AM) to provide a new theoretical basis for its treatment.</p><p><strong>Methods: </strong>Bulk RNA sequencing was employed to analyze samples from AM patients, and identify differentially expressed genes. Subsequently, bioinformatics methods such as Weighted Gene Co-Expression Network Analysis (WGCNA), DESeq2, and KEGG enrichment analysis were utilized to construct gene co-expression networks and identify pathways associated with invasion and recurrence. Furthermore, in vitro experiments, including Cell Counting Kit-8 (CCK-8), Wound healing assays, Western blotting, and qPCR were conducted to validate the effects of Wnt signaling on AM biological functions and the expression of related genes and proteins.</p><p><strong>Results: </strong>Bioinformatics analysis revealed significant activation of the Wnt signaling pathway during AM invasion and recurrence, and differential gene analysis identified specific gene expression patterns associated with the Wnt signaling pathway. In vitro experiments further demonstrated that the standard Wnt/β-catenin pathway activator, Laduviglusib significantly activated Wnt signaling, leading to a marked increase in the mRNA and protein expression levels of TCF7, β-catenin, WNT2B, and LEF1, thereby enhancing the proliferation and migration capabilities of AM cells.</p><p><strong>Conclusion: </strong>This study reveals the critical role of aberrant Wnt signaling activation in AM proliferation and migration, identifying it as a key driver of AM invasion and recurrence. The findings provide new insights into the mechanisms underlying AM invasion and recurrence, laying the foundation for developing novel therapeutic strategies.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"24 1","pages":"1421"},"PeriodicalIF":2.6,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142685949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}