BMC Oral Health最新文献

{"title":"Evaluation of dentists' malpractice fears and defensive dentistry attitudes: a scale development.","authors":"Selcen Eser Misir, Uğur Uğrak, Kübra Gülnur Topsakal, Perihan Dalgali, Gökhan Serhat Duran, Serkan Görgülü","doi":"10.1186/s12903-025-06103-1","DOIUrl":"10.1186/s12903-025-06103-1","url":null,"abstract":"<p><strong>Objectives: </strong>To develop and design the Defensive Dentistry Attitude Scale (DDAS) to evaluate the relationship between defensive dentistry and fear of malpractice among dentists in Türkiye.</p><p><strong>Methods: </strong>The Defensive Dentistry Assessment Scale (DDAS) questions were determined based on expert opinions and a literature review. Based on these results, a questionnaire including the 13-item DDAS and the malpractice fear scale was sent to 3513 dentists in Türkiye by email between 3.11.2023 and 10.1.2024. A total of 369 dentists returned. The questionnaire consisted of 26 questions with two VAS scales related to the frequency of complications and detailed anamnesis. During the development of DDAS, the Content Validity Index (CVI), Exploratory Factor Analysis (EFA), and Confirmatory Factor Analysis (CFA) were utilized. The Mann-Whitney U and Kruskal-Wallis tests were employed for group comparisons. In examining the relationships between variables, path analysis within the structural equation modeling (SEM) framework was conducted.</p><p><strong>Results: </strong>The DDAS developed in the study was a valid and reliable measure with two dimensions and eight items. DDAS (Median = 29) and malpractice fear scores (Median = 21) were high in dentists. Malpractice fear scores of dentists aged 29-35 years (Median = 21) were found to be statistically significantly higher than those of dentists aged 36 years and older (p < 0.05). The levels of malpractice fears (Median = 22) and defensive dentistry attitudes (Median = 31) of dentists with 6-10 years of experience were statistically significantly higher than those of dentists with 11 years of experience and over (respectively (Median = 20), (Median = 28), p < 0.05). A statistically significant and high-level positive correlation was found between dentists' fear of malpractice and their tendency to adopt defensive dentistry practices (β = 0.56, p < 0.001).</p><p><strong>Conclusions: </strong>According to the results of this study, the DDAS was found to be a valid and reliable measure of negative and positive defensive dentistry. Specifically, a significant and strong positive correlation was identified between dentists' fear of malpractice and their engagement in defensive dentistry practices. This finding indicates that the tendency to adopt defensive dental behaviors increases as malpractice fear increases.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"730"},"PeriodicalIF":2.6,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12083101/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Penetration of Biodentine, NeoMTA 2, and NeoPUTTY into dentinal tubules in primary tooth pulpotomy: a confocal laser scanning microscopy analysis.","authors":"Merve Ozdemir, Aysenur Oncu","doi":"10.1186/s12903-025-06065-4","DOIUrl":"10.1186/s12903-025-06065-4","url":null,"abstract":"<p><strong>Background: </strong>Pulpotomy is an accepted treatment option to preserve primary teeth with exposed pulp due to caries or trauma and to ensure proper function until physiologic exfoliation. This study aimed to assess the dentin penetration of NeoMTA 2, NeoPUTTY, and Biodentine using confocal laser scanning microscopy (CLSM).</p><p><strong>Methods: </strong>The present study was performed using 42 freshly extracted lower primary molars. All samples were divided into 3 separate groups and the Biodentine, NeoMTA 2, and NeoPUTTY were placed on the cavity floor, respectively. High-speed diamond discs were used to create 2-mm-thick sections from the furcation region, including a 1- mm crown and 1-mm root. Primary molar tooth sections were placed on a glass slide and examined using a CLSM (LSM 980, Zeiss, Germany). All images were analyzed using ImageJ/Fiji (National Institute of Health, USA) software for the measurement of penetration depth. The penetration depth of the materials was evaluated using one- way ANOVA, and the Tukey test was used for comparisons between groups at p < 0.05 significance level.</p><p><strong>Results: </strong>There was no significant difference in penetration depth between the Biodentine and NeoMTA 2 materials. NeoPUTTY showed higher penetration depth than Biodentine and NeoMTA 2.</p><p><strong>Conclusions: </strong>This study reported that NeoPUTTY showed higher dentinal tubule penetration in primary teeth than NeoMTA 2 and Biodentine as pulpotomy materials.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"727"},"PeriodicalIF":2.6,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12083007/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hierarchical clustering analysis & machine learning models for diagnosing skeletal classes I and II in German patients.","authors":"Eva Paddenberg-Schubert, Kareem Midlej, Sebastian Krohn, Iqbal M Lone, Osayd Zohud, Obaida Awadi, Samir Masarwa, Christian Kirschneck, Nezar Watted, Peter Proff, Fuad A Iraqi","doi":"10.1186/s12903-025-06063-6","DOIUrl":"10.1186/s12903-025-06063-6","url":null,"abstract":"<p><strong>Background: </strong>Classification is one of the most common tasks in artificial intelligence (AI) driven fields in dentistry and orthodontics. The AI abilities can significantly improve the orthodontist's critical mission to diagnose and treat patients precisely, promptly, and efficiently. Therefore, this study aims to develop a machine-learning model to classify German orthodontic patients as skeletal class I or II based on minimal cephalometric parameters. Eventually, clustering analysis was done to understand the differences between clusters within the same or different skeletal classes.</p><p><strong>Methods: </strong>A total of 556 German orthodontic patients were classified into skeletal class I (n = 210) and II (n = 346) using the individualized ANB. Hierarchical clustering analysis used the Euclidean distances between data points and Ward's minimum variance method. Six machine learning models (random forest (RF), K-nearest neighbor (KNN), support vector machine (SVM), linear discriminant analysis (LDA), classification and regression trees (CART), and General Linear Model (GLM)) were evaluated considering their accuracy, reliability, sensitivity, and specificity in diagnosing skeletal class I and II.</p><p><strong>Results: </strong>The clustering analysis results showed the power of this tool to cluster the results into two-three clusters that interestingly varied significantly in many cephalometric parameters, including NL-ML angle, NL-NSL angle, PFH/AFH ratio, gonial angle, SNB, Go-Me (mm), Wits appraisal, ML-NSL, and part of the dental parameters. The CART model achieved 100% accuracy by considering all cephalometric and demographic variables, while the KNN model performed well with three input parameters (ANB, Wits, SNB) only.</p><p><strong>Conclusions: </strong>The KNN model with three key variables demonstrated sufficient accuracy for classifying skeletal classes I and II, supporting efficient and still personalized orthodontic diagnostics and treatment planning. Further studies with balanced sample sizes are needed for validation.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"731"},"PeriodicalIF":2.6,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12083114/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the distribution of the dental hygienist workforce and population in Ontario: a geospatial analysis.","authors":"Mark J Dobrow, Eric Bruce, Keisha Simpson, Glenn Pettifer","doi":"10.1186/s12903-025-06092-1","DOIUrl":"10.1186/s12903-025-06092-1","url":null,"abstract":"<p><strong>Objectives: </strong>This study conducted a geospatial analysis of the distribution of the dental hygienist workforce relative to the distribution of the population in Ontario, Canada, aiming to address workforce imbalances and inform regional and international workforce planning.</p><p><strong>Methods: </strong>Geospatial analysis techniques were employed to examine the dental hygienist workforce distribution using anonymized datasets from the College of Dental Hygienists of Ontario (the professional regulatory body) and the Canadian census. The data were linked using the forward sortation area (FSA) component of Canadian postal codes, covering 520 FSAs across Ontario. Analyses were conducted at three levels, based on different aggregations of postal code data.</p><p><strong>Results: </strong>The study found significant variations in the distribution of dental hygienists across Ontario. The analysis revealed pockets of high dental hygienist density, mostly in urban areas, and areas with low dental hygienist rates, especially in rural and remote locations. The overall provincial rate was 97 dental hygienists per 100,000 population, with variation across the 520 FSAs, from zero to 20,000 dental hygienists per 100,000 population (or zero to 739 dental hygienists per 100,000 population if five outlier FSAs were removed).</p><p><strong>Conclusions: </strong>The study underscores the complexity of dental hygienist workforce distribution in Ontario, revealing significant geographical disparities that suggest areas of both oversupply and undersupply. These insights provide actionable guidance for workforce policies and regulatory strategies, such as targeted incentives and public health initiatives, to address the mismatch between workforce supply and population needs. The findings highlight the importance of regular geospatial analyses to track changes in workforce distribution over time. The rigorous methodological approach and comprehensive evaluation of potential limitations offer valuable guidance for similar analyses in other jurisdictions. By providing a detailed framework and insights that extend beyond Ontario, this study contributes to the global understanding of dental hygienist workforce dynamics and supports the development of informed policies on a broader scale.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"734"},"PeriodicalIF":2.6,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12083121/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical outcomes of nonsurgical root canal treatment using C-root SP combined with different obturation techniques in older patients: a randomized controlled clinical trial.","authors":"Liping Dong, Jianshe Li, Peng Xue, Suixin Hu, Chen Xu, Lin Wang, Liu Liu, Qiang Luo, Huixia He, Fei Chen","doi":"10.1186/s12903-025-06117-9","DOIUrl":"10.1186/s12903-025-06117-9","url":null,"abstract":"<p><strong>Background: </strong>To evaluate the clinical efficacy and outcomes of C-Root SP bioceramic-based sealers combined with different obturation techniques in nonsurgical root canal treatment for older patients.</p><p><strong>Methods: </strong>This prospective, single-center, single-blind, parallel, randomized clinical trial included 240 patients aged ≥ 60 years. Patients were randomly divided into four treatment groups. Group A: C-Root SP combined with single-cone (SC); Group B: C-Root SP combined with warm vertical compaction (WVC); Group C: C-Root SP combined with cold lateral condensation (CLC); Group D: iRoot SP combined with SC (Control). All teeth were subjected to root canal filling using the corresponding methods 7-10 days after root canal preparation and medication. The duration of obturation was recorded. Periapical radiographs were obtained immediately after obturation to determine the length of root canal filling, and postoperative pain on days 1, 2, and 7 was documented. The treatment outcomes based on clinical signs and symptoms as well as periapical index (PAI) were analyzed at 1 year postoperatively.</p><p><strong>Results: </strong>Groups A and D exhibited significantly higher filling efficiency than groups B and C (P < 0.001). No significant differences were observed in the length of obturation between the groups (P > 0.05). Postoperative pain decreased over time in all groups, with group A showing significantly lower pain rates on day 1 than the other groups (P < 0.05). After 1 year, all groups exhibited considerable reductions in the PAI scores, with success rates ranging from 91.23 to 94.83%; however, no significant differences were observed between them (P > 0.05).</p><p><strong>Conclusions: </strong>C-Root SP combined with different obturation techniques yielded similar outcomes to iRoot SP combined with the SC technique in terms of filling length, success rate, and changes in PAI scores. The SC technique demonstrated significant advantages in terms of clinical efficiency and may reduce early postoperative pain when combined with C-Root SP.</p><p><strong>Trial registration: </strong>The study was retrospectively registered in chictr.org.cn with the identifier: ChiCTR2400092580 on 11/19/2024.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"728"},"PeriodicalIF":2.6,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12082915/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unravelling the role of interleukin-6 in regulating dental stem cell behaviour: a scoping review.","authors":"Itt Assoratgoon, Nunthawan Nowwarote, Suphalak Phothichailert, Waruna L Dissanayaka, Lakshman P Samaranayake, Thanaphum Osathanon","doi":"10.1186/s12903-025-06097-w","DOIUrl":"10.1186/s12903-025-06097-w","url":null,"abstract":"<p><strong>Background: </strong>Interleukin-6 (IL-6) functions as a pro-inflammatory and anti-inflammatory cytokine. IL-6 plays a pivotal role in a multitude of biological processes. This scoping review aimed to explore the impact of IL-6 on the biological responses of dental tissue-derived mesenchymal stem cells.</p><p><strong>Methods: </strong>The literature databases (PubMed and Scopus) were searched utilising specific design keywords. The criteria for inclusion encompassed (1) original research investigations that examined the effect of IL-6 on dental stem cells through the introduction of exogenous IL-6, overexpression, or knockdown expression, and (2) publications authored in the English language. The articles that conformed to these criteria were subsequently compiled for comprehensive full-text analysis and data extraction.</p><p><strong>Results: </strong>The literature search identified 323 articles, including 99 and 224 pertinent publications from the PubMed and Scopus databases, respectively. After screening, 14 publications satisfied the inclusion criteria and were subsequently selected for data extraction. The findings demonstrated that IL-6 regulates stemness maintenance, cellular proliferation, and differentiation towards osteogenic, adipogenic, chondrogenic, and neurogenic lineages in dental tissue-derived mesenchymal stem cells.</p><p><strong>Conclusion: </strong>IL-6 modulates the biological activities of mesenchymal stem cells, particularly those derived from dental tissues. Nevertheless, the inconsistencies observed across various studies may be attributable to a range of factors, including the heterogeneity inherent in stem cell sources, the stages of differentiation, the conditions of culture, and the particularities of experimental design.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"732"},"PeriodicalIF":2.6,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12082909/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Measurement of orthodontic tooth movement in lower anterior teeth by means of magnetic resonance imaging- a prospective pilot study.","authors":"Linda Schwarz, Marina Stanley, André Gahleitner, Ewald Unger, Erwin Jonke, Xiaohui Rausch-Fan","doi":"10.1186/s12903-025-06073-4","DOIUrl":"10.1186/s12903-025-06073-4","url":null,"abstract":"<p><strong>Background: </strong>During orthodontic treatment, the risk of gingival recession may increase, especially in the mandibular anterior region due to thin alveolar bone. This prospective pilot study aimed to develop a standardized evaluation method based on magnetic resonance imaging (MRI) for analysing tooth movements and tissue dimensions and to investigate the impact on periodontal parameters during orthodontic levelling.</p><p><strong>Materials and methods: </strong>Participants aged 12 to 18 with lower jaw crowding underwent MRI scans before (T0) and five months into orthodontic treatment (T1). The following tissue dimensions were analysed: thickness of free and supracrestal gingiva (FGT, SGT), thickness of buccal alveolar bone (ABT) at three measurement levels (ABT2, ABT4, ABT8), and gingiva and alveolar bone height (GH, ABH). Additionally, tooth positions (apex position and tooth axis inclination) were determined.</p><p><strong>Results: </strong>Ten patients (60% female, 40% male) aged 14.33 ± 1.35 years were included after the exclusion of 3 datasets due to motion artefacts. MRI measurements showed significant changes in tooth inclination (2.93 ± 4.77°, p < 0.001), bucco-lingual apex position (-0.45 ± 1.03 mm, p = 0.006), SGT (-0.07 ± 0.19 mm, p = 0.020), ABT8 (0.42 ± 0.59 mm, p < 0.001), ABH (-0.29 ± 0.68 mm, p = 0.006) and GH (0.31 ± 0.9 mm, p = 0.030) between T0 and T1. Inclination changes correlated negatively with FGT (R = -0.422, p < 0.001) and positively with ABT8 (R = 0.404, p = 0.032). Furthermore, ABT8 correlated negatively with buccal apex movement (R=-0.392, p = 0.042). Intra- and interclass correlation coefficients were excellent (0.988 and 0.975).</p><p><strong>Conclusion: </strong>Periodontal tissue changes correlated with tooth inclination or apex position changes due to orthodontic treatment. The pilot study has demonstrated the feasibility of dental MRI as a radiation-free alternative to cone-beam computed tomography for monitoring orthodontic treatment. However, the methodology was susceptible to motion artefacts.</p><p><strong>Trial registration: </strong>ISRCTN, ISRCTN12689212. Registered 11 April 2024 Retrospectively registered, http//www.isrctn.com/ISRCTN12689212 .</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"726"},"PeriodicalIF":2.6,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12079835/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of exposure of teeth to Ph change of chlorinated water on shear bond strength of metal orthodontic brackets (an in vitro study).","authors":"Eman A Mustafa, Seham A Hanafy, Tarek N Yousry, Hanan A Ismail","doi":"10.1186/s12903-025-06100-4","DOIUrl":"10.1186/s12903-025-06100-4","url":null,"abstract":"<p><strong>Background: </strong>Swimming is widely recognized as one of the healthiest forms of exercise, but chlorinated water in swimming pools can adversely affect orthodontic bracket bonding. The objective of this study was to determine the effects of chlorinated water with varying pH levels on the shear bond strength (SBS) of metal brackets and to assess the adhesive remnant index (ARI) following bracket debonding.</p><p><strong>Materials and methods: </strong>A total of 126 sound premolars (71 maxillary and 55 mandibular) were randomly divided into three experimental groups: two test groups and one control group. In the test groups (Groups 1 and 2), the teeth were soaked in chlorinated water at two different pH values (pH 7.4 and pH 3), whereas in the control group (Group 3), the teeth were soaked in artificial saliva (pH 7). The soaking period lasted for 12 days to simulate one year of swimming training. A consistent bonding protocol was applied for all the samples. Each group was further randomly divided into three subgroups of 14 premolars to compare the SBS and ARI values immediately after bonding (Subgroup 1), after 6 days of bonding (mimicking 6 months of training) (Subgroup 2), and after 12 days of bonding (mimicking one year of training) (Subgroup 3).</p><p><strong>Results: </strong>In all the subgroups, the control group consistently had a higher SBS than did the test groups. Compared with Group 1, Group 2 had a significantly lower SBS. Specifically, the means and standard deviations in Group 1 were 7.34 ± 0.99 MPa for Subgroup 1, 6.89 ± 0.95 MPa for Subgroup 2, and 5.59 ± 1.09 MPa for Subgroup 3. In Group 2, the values were 6.12 ± 0.72 MPa for Subgroup 1, 5.82 ± 0.70 MPa for Subgroup 2, and 4.52 ± 0.86 MPa for Subgroup 3. Conversely, Group 3 presented means and standard deviations of 9.01 ± 0.99 MPa for Subgroup 1, 9.06 ± 0.91 MPa for Subgroup 2, and 9.10 ± 0.92 MPa for Subgroup 3. The ARI values were not significantly different between the groups.</p><p><strong>Conclusion: </strong>The pH of chlorinated swimming pool water affects the bond strength of orthodontic brackets, with a more acidic pH resulting in diminished bond strength. Accordingly, continuous monitoring of the pH of swimming pool water is essential.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"733"},"PeriodicalIF":2.6,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12083127/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral health in inflammatory bowel disease: the overlooked impact and the potential role of salivary calprotectin.","authors":"Cláudio Rodrigues, Ana T P C Gomes, Joana Leal, Pedro Pereira, Pedro C Lopes, Karina Mendes, Maria J Correia, Nélio Veiga, Nuno Rosa, Caroline Soares, Paula Ministro","doi":"10.1186/s12903-025-06064-5","DOIUrl":"10.1186/s12903-025-06064-5","url":null,"abstract":"<p><strong>Background: </strong>Inflammatory Bowel Disease (IBD), a chronic condition characterized by gastrointestinal inflammation, is influenced by genetic and environmental factors. Emerging evidence suggests a \"mouth-gut axis,\" with the oral cavity reflecting extra-intestinal manifestations of IBD. This study evaluated the oral health status of IBD patients and the potential of salivary calprotectin (SCP) as a biomarker for assessing IBD activity and oral health.</p><p><strong>Methods: </strong>Oral health was assessed in 100 IBD patients [60 with Crohn's disease (CD) and 40 with ulcerative colitis (UC)] and 14 controls. Evaluations included the Decayed, Missing, and Filled Teeth (DMFT) Score, Periodontal Diagnosis and the need for dental or prosthetic treatment. Saliva and stool samples were collected to measure SCP and faecal calprotectin (FCP) levels using the Elia Calprotectin 2 Test. IBD activity was evaluated with FCP, the Harvey-Bradshaw Index for CD, and the Partial Mayo Score for UC.</p><p><strong>Results: </strong>The DMFT index mean was comparable between IBD patients (mean 7.99, SD 7.73) and controls (mean 10.00, SD 6.49). However, periodontal disease was significantly more prevalent in IBD patients (57% in CD, 70% in UC) than in controls (29%), with severe cases (stages III/IV) more frequent in IBD. Additionally, 89% of IBD patients required dental treatment, and 39% needed prosthetic rehabilitation. SCP levels showed no significant correlation with disease activity or oral health status, while FCP correlated with C-reactive protein and erythrocyte sedimentation rate.</p><p><strong>Conclusions: </strong>This study underscores the need for improved oral health management in IBD patients and suggests that SCP may not be a reliable biomarker for monitoring IBD or periodontal disease.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"729"},"PeriodicalIF":2.6,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12082920/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of AMY1 gene copy number variation in dental caries susceptibility: insights from a Turkish population.","authors":"Ömer Hatipoğlu, Faruk Saydam","doi":"10.1186/s12903-025-06074-3","DOIUrl":"https://doi.org/10.1186/s12903-025-06074-3","url":null,"abstract":"<p><strong>Objective: </strong>Dental caries is a multifactorial disease influenced by environmental, behavioral, and genetic factors. Recent studies suggest that variations in the AMY1 gene, which encodes salivary amylase, may contribute to caries susceptibility. This study investigates the relationship between AMY1 gene copy number variation (CNV) and dental caries in a Turkish population.</p><p><strong>Method: </strong>A total of 154 participants (63% female; mean age 19.6 ± 1.4 years) were included. Epithelial cells in inner cheek tissues were collected from volunteers using swabs, and the collected samples were preserved and stored in a DNA stabilization solution. The demographic characteristics of the volunteers were recorded, and DMFT and DMFS index scores were documented on the provided forms. The AMY1 gene CNVs were determined using a Real-time polymerase reaction device. The TaqMan chemistry, which comprises quantitative real-time PCR reactions utilizing a dual TaqMan kit, was utilized in this analysis process. Statistical analyses included the Kruskal-Wallis and Mann-Whitney U tests for group comparisons, Spearman's correlation analysis, and binomial logistic regression to evaluate associations between AMY1 CNVs and dental caries indices.</p><p><strong>Results: </strong>No statistically significant differences were observed between AMY1 CNVs and DMFT or DMFS indices (p > 0.05). However, significant associations were found between daily tooth brushing frequency and caries indices (Cramer's V = 0.219, p < 0.05), as well as between preferred beverage consumption and caries indices (Cramer's V = 0.219, p < 0.05). Other factors, including gender, dental floss and mouthwash use, and tongue brushing, did not show statistically significant associations (p > 0.05).</p><p><strong>Conclusion: </strong>Although AMY1 CNVs were not significantly associated with caries indices, the findings highlight the complex interplay of genetic, behavioral, and dietary factors in caries development. This study emphasizes the critical role of oral hygiene and dietary habits in caries prevention and underscores the need for further research on genetic contributions to oral health.</p><p><strong>Clinical trial number: </strong>Not applicable.</p>","PeriodicalId":9072,"journal":{"name":"BMC Oral Health","volume":"25 1","pages":"722"},"PeriodicalIF":2.6,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12079818/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}