The open analytical chemistry journal最新文献_第3页

Practical considerations in the pharmaceutical analysis of methyl and hexyl ester derivatives of 5-aminolevulinic acid 5-氨基乙酰丙酸甲酯和己酯衍生物药物分析中的实际考虑

The open analytical chemistry journal Pub Date : 2009-07-28 DOI: 10.2174/1874065000903010006

D. I. Morrow, P. McCarron, A. Woolfson, R. Donnelly

{"title":"Practical considerations in the pharmaceutical analysis of methyl and hexyl ester derivatives of 5-aminolevulinic acid","authors":"D. I. Morrow, P. McCarron, A. Woolfson, R. Donnelly","doi":"10.2174/1874065000903010006","DOIUrl":"https://doi.org/10.2174/1874065000903010006","url":null,"abstract":"Photodynamic therapy (PDT) using topical 5-aminolevulinic acid (ALA), a water-soluble precursor of the potent endogenous photosensitiser, protoporphyrin IX (PpIX), is a treatment and diagnostic tool for premalignant and malignant skin cancers. However, to improve drug delivery to deeper skin lesions, more lipophilic ALA esters have been investigated. Owing to the necessity in drug delivery research for efficient and validated assays for ALA esters in solution, this paper aims to describe optimised protocols to quantify the methyl and hexyl esters of ALA. ALA esters were derivatised using acetyl acetone and formaldehyde reagents and analysed using reversed phase HPLC. For the first time, the significance of ALA-impurities in ester samples has been highlighted. Furthermore, it was shown that for a given concentration, peak areas obtained for ALA-esters were significantly smaller than those obtained for ALA (p m-ALA (38.3%) > h-ALA (33.9%). These findings may explain why, historically, some of the benefits seen with ALA-esters using cell culture models have not been demonstrated in vivo.","PeriodicalId":90363,"journal":{"name":"The open analytical chemistry journal","volume":"110 1","pages":"6-15"},"PeriodicalIF":0.0,"publicationDate":"2009-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76109841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Quantitative Simultaneous Estimation of Water Soluble Vitamins,Riboflavin, Pyridoxine, Cyanocobalamin and Folic Acid in NeutraceuticalProducts by HPLC 高效液相色谱法同时定量测定中性保健品中水溶性维生素、核黄素、吡哆醇、氰钴胺素和叶酸

The open analytical chemistry journal Pub Date : 2009-04-21 DOI: 10.2174/1874065000903010001

S. Perveen, Arfa Yasmina, K. Khan

引用次数: 21

Direct and Derivative Spectrophotometric Determination of Copper(II) with 5-Bromosalicylaldehyde Thiosemicarbazone 5-溴水杨醛硫代氨基脲直接及导数分光光度法测定铜(II)

The open analytical chemistry journal Pub Date : 2009-01-02 DOI: 10.2174/1874065000802010078

G. Ramanjaneyulu, P. R. Reddy, V. Reddy, T. Reddy

引用次数: 18

High-Performance Liquid Chromatographic Quantitative Determination of Amcinonide and Benzyl Alcohol in Pharmaceutical Preparations 高效液相色谱法测定药物制剂中氨苄胺和苯甲醇的含量

The open analytical chemistry journal Pub Date : 2008-09-02 DOI: 10.2174/1874065000802010074

K. Khan, Abdul Rafiq Khan, S. Perveen, Z. Ullah, N. Ambreen, W. Voelter

引用次数: 3

Capillary Electrophoresis as Analytical Method for the Characterisation of Natural Diterpenoid Resins in Artistic and Historic Works: A Comparison with Gas Chromatography 毛细管电泳作为鉴定艺术和历史作品中天然二萜树脂的分析方法:与气相色谱法的比较

The open analytical chemistry journal Pub Date : 2008-06-26 DOI: 10.2174/1874065000802010067

Madeleine Dellmour, A. Findeisen, Isabella Kaml, W. Baatz, E. Kenndler

{"title":"Capillary Electrophoresis as Analytical Method for the Characterisation of Natural Diterpenoid Resins in Artistic and Historic Works: A Comparison with Gas Chromatography","authors":"Madeleine Dellmour, A. Findeisen, Isabella Kaml, W. Baatz, E. Kenndler","doi":"10.2174/1874065000802010067","DOIUrl":"https://doi.org/10.2174/1874065000802010067","url":null,"abstract":"We have applied capillary electrophoresis to separate and detect the characteristic main components of diterpenoic resins applied as binders in museum objects, i.e. the abietane and pimarane acids and larixol acetate. Under the influence of the applied high voltage of 15000 V these analytes move in a narrow separation capillary with 50 m inner diameter. This open tube has 48.5 cm total length, with 40.0 cm distance to the detector. Electrophoretic migration is supported (and, in case of the electrically neutral larixol acetate, enabled) by charged additives to the running buffer, which form ionic complexes with the analytes. The separated analytes are detected within less than 15 min by their UV absorbance measured at characteristic wavelengths of 200, 250 and 270 nm. In contrast to chromatographic methods, no derivatisation is needed. As in gas chromatography, identification of the resins is carried out by the peak patterns based on the resin composition. From the comparison with established GC methodology for the analysis of resins it can be concluded that the analytical performance parameters of the capillary electrophoretic method in terms of resolution and analysis time are comparable to those from gas chromatography, whereas the detection limit is higher in general. However, sample pretreatment is simpler for capillary electrophoresis; it consists only in the dissolution of the sample in methanol.","PeriodicalId":90363,"journal":{"name":"The open analytical chemistry journal","volume":"7 1","pages":"67-73"},"PeriodicalIF":0.0,"publicationDate":"2008-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81574838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Direct Injection of Redissolved Cell Culture Media into a Single-Column Liquid Chromatography Coupled to Mass Spectrometry for the Measurement of PGE2 单柱液相色谱-质谱联用法直接注入溶解细胞培养基测定PGE2

The open analytical chemistry journal Pub Date : 2008-06-06 DOI: 10.2174/1874065000802010062

P. Araujo, Z. Du, Thu-Thao Nguyen, E. Holen

{"title":"Direct Injection of Redissolved Cell Culture Media into a Single-Column Liquid Chromatography Coupled to Mass Spectrometry for the Measurement of PGE2","authors":"P. Araujo, Z. Du, Thu-Thao Nguyen, E. Holen","doi":"10.2174/1874065000802010062","DOIUrl":"https://doi.org/10.2174/1874065000802010062","url":null,"abstract":"The traditional approach to assessing eicosanoids in cell cultures by liquid or gas chromatography coupled to mass spectrometry involves time-consuming procedures such as derivatisation, degradation, solid-phase extraction, liquid- liquid extraction, thin layer chromatography and sometimes, combination of all these procedures prior to the injection of the sample in the chromatography system. The performance of a method that allows the direct injection of redissolved cell culture media into a single-column liquid chromatography instrument coupled to mass spectrometry is evaluated. The ex- ternal standard calibration curves were linear between 1-50 ng/ml for Williams' medium E and L-15 medium. The limit of detection and quantification were 0.5 and 1 ng/ml in both media respectively. The recovery values were 98.6% for Wil- liams' medium E and 100.3% for L-15 medium. The internal standard method was not used for quantitative purposes due to the variability of the response factor. The proposed method has potential for broad implementation in monitoring bio- markers in cell cultures.","PeriodicalId":90363,"journal":{"name":"The open analytical chemistry journal","volume":"6 1","pages":"62-66"},"PeriodicalIF":0.0,"publicationDate":"2008-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82527310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Case Study for Selected Heavy Elements in Blood and Plasma from Six Female Patients with Systemic Sclerosis (Scleroderma, SSc). The Concentrations of Mercury, Lead, Antimony, Cobalt, Copper, and Zinc 6例女性系统性硬化症(硬皮病，SSc)患者血液和血浆中选定重元素的案例研究。汞、铅、锑、钴、铜和锌的浓度

The open analytical chemistry journal Pub Date : 2008-05-09 DOI: 10.2174/1874065000802010055

G. Tamasi, M. Bellini, S. Lorenzini, D. Chindamo, R. Marcolongo, F. Baroni, F. Riccobono, R. Netti, R. Cini

{"title":"Case Study for Selected Heavy Elements in Blood and Plasma from Six Female Patients with Systemic Sclerosis (Scleroderma, SSc). The Concentrations of Mercury, Lead, Antimony, Cobalt, Copper, and Zinc","authors":"G. Tamasi, M. Bellini, S. Lorenzini, D. Chindamo, R. Marcolongo, F. Baroni, F. Riccobono, R. Netti, R. Cini","doi":"10.2174/1874065000802010055","DOIUrl":"https://doi.org/10.2174/1874065000802010055","url":null,"abstract":"Samples of total blood (1T-6T) were collected from six patients (1-6) with Systemic Sclerosis (SSc) and ana- lyzed for their content of mercury, lead, antimony, cobalt, copper, and zinc through AASGF (atomic absorption spectros- copy - graphite furnace), HR-ICP-MS (high resolution inductively-coupled plasma mass spectroscopy) and CVAAT (cold vapor atomic absorption technique). The blood samples (7CT, 8CT) from two \"healthy subjects\" (7, 8) were also col- lected and analyzed. Furthermore, plasma samples (1P-6P, and 7CP and 8CP) were prepared from fresh blood samples and analyzed for the same elements. The summary for Hg, usually considered highly toxic, is as follows. Interestingly, three of six total blood samples had concentrations of mercury (1T, 11.0(4) μg/L; 2T, 8.2(3); 4T, 7.4(3)) significantly higher than the control from this work (5.6(6) μg/L) and the control from the literature (5.3 μg/L). The concentration of lead for the total blood samples is in the range 82.7(5) - 36.9(5) μg/L and agrees well with average values for women from literature, 70 μg/L. The average concentration for the essential element zinc for the samples 1T-6T is 6658 μg/L, which compares well with the mean value from literature (5561 μg/L for \"healthy subjects\").","PeriodicalId":90363,"journal":{"name":"The open analytical chemistry journal","volume":"23 1","pages":"55-61"},"PeriodicalIF":0.0,"publicationDate":"2008-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90257241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Development and Characterization of a Solid Phase for Single-Step Enrichment of Pathogenic Targets 用于病原菌单步富集的固体相的开发与表征

The open analytical chemistry journal Pub Date : 2008-05-09 DOI: 10.2174/1874065000802010047

M. Archer, D. Stenger, B. Lin

引用次数: 3

A Comparative Study of Metal Readsorption in the Application of a Three-Stage Sequential Extraction Scheme and Two Accelerated Versions (Ultrasonic and Single Extractions) 三段顺序萃取和两种加速萃取(超声和单次萃取)对金属再吸附的比较研究

The open analytical chemistry journal Pub Date : 2008-04-29 DOI: 10.2174/1874065000802010040

G. Pérez, M. Valiente, C. Bendicho

{"title":"A Comparative Study of Metal Readsorption in the Application of a Three-Stage Sequential Extraction Scheme and Two Accelerated Versions (Ultrasonic and Single Extractions)","authors":"G. Pérez, M. Valiente, C. Bendicho","doi":"10.2174/1874065000802010040","DOIUrl":"https://doi.org/10.2174/1874065000802010040","url":null,"abstract":"Accelerated methods for partitioning of Cd, Cr, Cu, Ni, Pb and Zn, such as the use of single and ultrasonic ex- tractions are assessed in terms of readsorption and compared with the three-stage sequential extraction scheme (SES) of the Standards Measurements and Testing Programme (SM&T). The standard addition approach was employed for charac- terising the readsorption artefact by applying the above fractionation methods over different certified reference materials (CRMs), BCR 701, BCR 601 (lake sediments), and BCR 141R soil. Ultrasonic extractions provided higher readsorption, mainly for BCR 141R and 601 as compared to conventional SES, the role of ultrasound in the activation of adsorptive sites being significant. The single extraction approach seemed to be inadequate with samples containing large amounts of carbonates such as BCR 141 R but worked well with both lake sediments. The readsorption phenomena are mainly occurring in the most labile fraction (i.e. acid soluble) and has been observed to be matrix dependent. The extent of such phenomenon is also dependent on the extraction methodology, i.e conventional vs accelerated.","PeriodicalId":90363,"journal":{"name":"The open analytical chemistry journal","volume":"18 1","pages":"40-46"},"PeriodicalIF":0.0,"publicationDate":"2008-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90795878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Ultrasensitive Bioanalytical Assays for Cytotoxic Drugs: Focus on Locally Administered Anti-Cancer Agents 细胞毒性药物的超灵敏生物分析测定:重点是局部给药的抗癌药物

The open analytical chemistry journal Pub Date : 2008-03-18 DOI: 10.2174/1874065000802010010

L. Vainchtein, H. Rosing, J. Schellens, J. Beijnen

引用次数: 1