The open analytical chemistry journal最新文献_第4页

Monitoring of Aquatic Environments Using Passive Samplers 使用被动采样器监测水生环境

The open analytical chemistry journal Pub Date : 2008-01-16 DOI: 10.2174/1874065000802010001

L. Chimuka, E. Cukrowska, H. Tutu

引用次数: 8

Progress in Silica Chemistry - Determination of Physico-Chemical Parameters via Near-Infrared Diffuse Reflection Spectroscopy 二氧化硅化学研究进展。用近红外漫反射光谱法测定理化参数

The open analytical chemistry journal Pub Date : 2007-11-02 DOI: 10.2174/1874065000701010021

C. Huck, N. Heigl, M. Najam-ul-Haq, M. Rainer, R. Vallant, G. Bonn

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引用次数: 6

Study on Lipid Fractions of Streptococcus thermophilus by TLC, GC and GC/MS Techniques TLC、GC和GC/MS技术对嗜热链球菌脂质组分的研究

The open analytical chemistry journal Pub Date : 2007-10-30 DOI: 10.2174/1874065000701010015

V. Pelizzola, M. Povolo, A. Avalli, B. Bottari, E. Neviani, G. Contarini

引用次数: 3

Analytical Tools for Monitoring Arsenic in the Environment 监测环境中砷的分析工具

The open analytical chemistry journal Pub Date : 2007-10-04 DOI: 10.2174/1874065000701010007

J. Luong, Ehsan Majid, K. Male

引用次数: 48

Protein-Conjugated Quantum Dots for Detecting Trypsin and Trypsin Inhibitor Through Fluorescence Resonance Energy Transfer 蛋白质共轭量子点荧光共振能量转移检测胰蛋白酶和胰蛋白酶抑制剂

The open analytical chemistry journal Pub Date : 2007-09-04 DOI: 10.2174/1874065000701010001

Chi-Wei Liu, Huan‐Tsung Chang

{"title":"Protein-Conjugated Quantum Dots for Detecting Trypsin and Trypsin Inhibitor Through Fluorescence Resonance Energy Transfer","authors":"Chi-Wei Liu, Huan‐Tsung Chang","doi":"10.2174/1874065000701010001","DOIUrl":"https://doi.org/10.2174/1874065000701010001","url":null,"abstract":"We have developed quantum dot probes for detecting trypsin (Try) and trypsin inhibitor (TI) in aqueous solu- tions through fluorescence resonance energy transfer (FRET). Green-fluorescent CdTe quantum dots (QDs) served as the energy donors and rhodamine isothiocyanate (RITC) conjugated to bovine serum albumin (BSA-RITC) was the acceptor. By simply mixing the two fluorophores, FRET occurred when BSA-RITC bound to the CdTe QDs; as a result, the fluo- rescence intensity of the CdTe QDs at 520 nm decreased, while the fluorescence of RITC at 574 nm increased. When Try was used to digest BSA, the FRET efficiency decreased, allowing the detection of Try at concentrations as low as 110 pM. In the presence of TI, the digestion activity of Try was inhibited. As a result, the fluorescence intensity ratio IF574/IF520 of the QD-BSA-RITC solutions in the presence of a constant amount of Try increased upon increasing the concentration of TI; good linearity (R 2 = 0.99) existed over the range 0.3-5.0 nM. The LOD for TI was 250 pM. This simple and cost- effective probe was applied to determine the level of spiked TI (1.0 nM) in urine samples; the recoveries (95-110%) sug- gested low matrix interference and high sensitivity.","PeriodicalId":90363,"journal":{"name":"The open analytical chemistry journal","volume":"3 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2007-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75857818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Purification of Proteins From Cell-Culture Medium or Cell-Lysate by High-Speed Counter-Current Chromatography Using Cross-Axis Coil Planet Centrifuge. 利用十字轴盘旋行星离心机的高速逆流色谱法从细胞培养液或细胞裂解液中纯化蛋白质。

The open analytical chemistry journal Pub Date : 2007-01-01 DOI: 10.2174/1874065000701010028

Yoichi Shibusawa, Yoichiro Ito

引用次数: 0