{"title":"利用十字轴盘旋行星离心机的高速逆流色谱法从细胞培养液或细胞裂解液中纯化蛋白质。","authors":"Yoichi Shibusawa, Yoichiro Ito","doi":"10.2174/1874065000701010028","DOIUrl":null,"url":null,"abstract":"<p><p>This review describes protein purifications from cell culture medium or cell-lysate by high speed counter-current chromatography using the cross-axis coil planet centrifuge. Purifications were performed using aqueous two phase systems composed of polyethylene glycols and dextrans.</p>","PeriodicalId":90363,"journal":{"name":"The open analytical chemistry journal","volume":"1 ","pages":"28-37"},"PeriodicalIF":0.0000,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4211269/pdf/nihms563433.pdf","citationCount":"0","resultStr":"{\"title\":\"Purification of Proteins From Cell-Culture Medium or Cell-Lysate by High-Speed Counter-Current Chromatography Using Cross-Axis Coil Planet Centrifuge.\",\"authors\":\"Yoichi Shibusawa, Yoichiro Ito\",\"doi\":\"10.2174/1874065000701010028\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This review describes protein purifications from cell culture medium or cell-lysate by high speed counter-current chromatography using the cross-axis coil planet centrifuge. Purifications were performed using aqueous two phase systems composed of polyethylene glycols and dextrans.</p>\",\"PeriodicalId\":90363,\"journal\":{\"name\":\"The open analytical chemistry journal\",\"volume\":\"1 \",\"pages\":\"28-37\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2007-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4211269/pdf/nihms563433.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The open analytical chemistry journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2174/1874065000701010028\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The open analytical chemistry journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2174/1874065000701010028","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Purification of Proteins From Cell-Culture Medium or Cell-Lysate by High-Speed Counter-Current Chromatography Using Cross-Axis Coil Planet Centrifuge.
This review describes protein purifications from cell culture medium or cell-lysate by high speed counter-current chromatography using the cross-axis coil planet centrifuge. Purifications were performed using aqueous two phase systems composed of polyethylene glycols and dextrans.