K. Khan, Abdul Rafiq Khan, S. Perveen, Z. Ullah, N. Ambreen, W. Voelter
{"title":"高效液相色谱法测定药物制剂中氨苄胺和苯甲醇的含量","authors":"K. Khan, Abdul Rafiq Khan, S. Perveen, Z. Ullah, N. Ambreen, W. Voelter","doi":"10.2174/1874065000802010074","DOIUrl":null,"url":null,"abstract":"This paper describes a simple, precise, and validated high-performance liquid chromatographic method for the simultaneous quantitative determination of amcinonide and benzyl alcohol in pharmaceutical preparations. The separation was achieved using a Zorbax reversed-phase column (5 m, 250x4.6mm) at room temperature with an isocratic mixture of deionized water and acetonitrile (70:30 v/v) at a flow rate of 1.5ml/min and detection at 254 nm. The limits of quantification for amcinonide and benzyl alcohol were found to be 4-30 g/ml and 80-600 g/ml, respectively. The validity of the method was evaluated in terms of linear regression analysis, precision, specificity, accuracy and ruggedness. The developed methodology is an economic, time-saving, straight forward and precise assay for the determination of amcinonide and benzyl alcohol in pharmaceutical preparations. It can be readily utilized for quality assurance and R&D laboratories of pharmaceutical and cosmetic industry.","PeriodicalId":90363,"journal":{"name":"The open analytical chemistry journal","volume":"1 1","pages":"74-77"},"PeriodicalIF":0.0000,"publicationDate":"2008-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"High-Performance Liquid Chromatographic Quantitative Determination of Amcinonide and Benzyl Alcohol in Pharmaceutical Preparations\",\"authors\":\"K. Khan, Abdul Rafiq Khan, S. Perveen, Z. Ullah, N. Ambreen, W. Voelter\",\"doi\":\"10.2174/1874065000802010074\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"This paper describes a simple, precise, and validated high-performance liquid chromatographic method for the simultaneous quantitative determination of amcinonide and benzyl alcohol in pharmaceutical preparations. The separation was achieved using a Zorbax reversed-phase column (5 m, 250x4.6mm) at room temperature with an isocratic mixture of deionized water and acetonitrile (70:30 v/v) at a flow rate of 1.5ml/min and detection at 254 nm. The limits of quantification for amcinonide and benzyl alcohol were found to be 4-30 g/ml and 80-600 g/ml, respectively. The validity of the method was evaluated in terms of linear regression analysis, precision, specificity, accuracy and ruggedness. The developed methodology is an economic, time-saving, straight forward and precise assay for the determination of amcinonide and benzyl alcohol in pharmaceutical preparations. It can be readily utilized for quality assurance and R&D laboratories of pharmaceutical and cosmetic industry.\",\"PeriodicalId\":90363,\"journal\":{\"name\":\"The open analytical chemistry journal\",\"volume\":\"1 1\",\"pages\":\"74-77\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2008-09-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The open analytical chemistry journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2174/1874065000802010074\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The open analytical chemistry journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2174/1874065000802010074","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
High-Performance Liquid Chromatographic Quantitative Determination of Amcinonide and Benzyl Alcohol in Pharmaceutical Preparations
This paper describes a simple, precise, and validated high-performance liquid chromatographic method for the simultaneous quantitative determination of amcinonide and benzyl alcohol in pharmaceutical preparations. The separation was achieved using a Zorbax reversed-phase column (5 m, 250x4.6mm) at room temperature with an isocratic mixture of deionized water and acetonitrile (70:30 v/v) at a flow rate of 1.5ml/min and detection at 254 nm. The limits of quantification for amcinonide and benzyl alcohol were found to be 4-30 g/ml and 80-600 g/ml, respectively. The validity of the method was evaluated in terms of linear regression analysis, precision, specificity, accuracy and ruggedness. The developed methodology is an economic, time-saving, straight forward and precise assay for the determination of amcinonide and benzyl alcohol in pharmaceutical preparations. It can be readily utilized for quality assurance and R&D laboratories of pharmaceutical and cosmetic industry.