高效液相色谱法测定药物制剂中氨苄胺和苯甲醇的含量

K. Khan, Abdul Rafiq Khan, S. Perveen, Z. Ullah, N. Ambreen, W. Voelter
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引用次数: 3

摘要

本文建立了一种简便、准确、有效的高效液相色谱法同时定量测定药物制剂中氨苄胺和苯甲醇的方法。采用Zorbax反相色谱柱(5 m, 250x4.6mm),去离子水和乙腈(70:30 v/v)等密度混合物,在室温下,流速为1.5ml/min,检测波长为254 nm。氨苄胺和苯甲醇的定量限分别为4 ~ 30 g/ml和80 ~ 600 g/ml。从线性回归分析、精密度、特异度、准确度和耐用性等方面评价了该方法的有效性。所开发的方法是一种经济、省时、直接和精确的测定药物制剂中氨苄胺和苯甲醇的方法。可方便地用于制药、化妆品行业的质量保证和研发实验室。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
High-Performance Liquid Chromatographic Quantitative Determination of Amcinonide and Benzyl Alcohol in Pharmaceutical Preparations
This paper describes a simple, precise, and validated high-performance liquid chromatographic method for the simultaneous quantitative determination of amcinonide and benzyl alcohol in pharmaceutical preparations. The separation was achieved using a Zorbax reversed-phase column (5 m, 250x4.6mm) at room temperature with an isocratic mixture of deionized water and acetonitrile (70:30 v/v) at a flow rate of 1.5ml/min and detection at 254 nm. The limits of quantification for amcinonide and benzyl alcohol were found to be 4-30 g/ml and 80-600 g/ml, respectively. The validity of the method was evaluated in terms of linear regression analysis, precision, specificity, accuracy and ruggedness. The developed methodology is an economic, time-saving, straight forward and precise assay for the determination of amcinonide and benzyl alcohol in pharmaceutical preparations. It can be readily utilized for quality assurance and R&D laboratories of pharmaceutical and cosmetic industry.
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