Current cellular biochemistry最新文献_第2页

Antioxidant Activity of Telang (Clitoria ternatea L.) Extract in Inhibiting Lipid Peroxidation 特朗(阴蒂)的抗氧化活性抑制脂质过氧化的提取物

Current cellular biochemistry Pub Date : 2022-06-10 DOI: 10.29244/cb.9.1.3

Ukhradiya Magharaniq Safira Purwanto, Kamaratih Aprilia, Sulistiyani

{"title":"Antioxidant Activity of Telang (Clitoria ternatea L.) Extract in Inhibiting Lipid Peroxidation","authors":"Ukhradiya Magharaniq Safira Purwanto, Kamaratih Aprilia, Sulistiyani","doi":"10.29244/cb.9.1.3","DOIUrl":"https://doi.org/10.29244/cb.9.1.3","url":null,"abstract":"Butterfly pea (Clitoria ternatea L.) has been widely used as a healthy drink as well as traditional medicine. This study aimed to test the antioxidant activity of C. ternatea aqueous extract based on its ability to inhibit lipid peroxidation (in vitro) using different parts and extraction methods. Extraction was carried out on flowers, leaves, and roots. The leaves and roots were extracted by boiling technique. Flowers were extracted in 3 ways: boiling, maceration, and brewing. Antioxidant activity was determined based on phenolic content, flavonoid content, and antioxidant test using the TBA method. The highest phenolic and flavonoid levels were shown by the aqueous extract of C. ternatea leave of 57.51 mg GAE/g and 1.50 mg EK/g, respectively. The highest levels of flavonoid were from flower parts treated with boiling technique (0.88 mg EK/g), followed by brewed flowers and macerated flowers. The highest antioxidant activity was obtained from flowers that were boiled for 30 minutes with a percentage inhibition of 94%. Based on the method of extraction and part of the plant, all extracts of C. ternatea have similar antioxidant potential.","PeriodicalId":90150,"journal":{"name":"Current cellular biochemistry","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75683087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Antibacterial Activity of Ethanol Extract of Red Betel Leaves (Piper crocatum) and Its Fractions against Escherichia coli pBR322 红槟榔叶乙醇提取物及其组分对大肠杆菌pBR322的抑菌活性研究

Current cellular biochemistry Pub Date : 2022-06-10 DOI: 10.29244/cb.9.1.1

Fernanda Chairunisa, M. Safithri, M. Bintang

引用次数: 1

Analysis of Metabolite Compound Profiles of Miana Leaves Endophytic Bacteria (Coleus scutellariodes) using GC-MS 用气相色谱-质谱法分析棉麻叶内生细菌代谢产物的化合物谱

Current cellular biochemistry Pub Date : 2022-02-26 DOI: 10.29244/cb.8.2.2

Dwi Endah Kusumawati, Ukhradiya Magharaniq Safira Purwanto, M. Bintang, F. Pasaribu

引用次数: 2

Potency of Bioactive Compounds in Indramayu Mango Peel Waste to Inhibit ACE2 Indramayu芒果皮废弃物中生物活性物质抑制ACE2的活性研究

Current cellular biochemistry Pub Date : 2021-12-06 DOI: 10.29244/cb.8.2.1

Mustika Luthfia, Assifah Eryandini, Deki Geraldi, Chelsy Narita, Choirunnisa Miftahul Jannah, L. Ambarsari

{"title":"Potency of Bioactive Compounds in Indramayu Mango Peel Waste to Inhibit ACE2","authors":"Mustika Luthfia, Assifah Eryandini, Deki Geraldi, Chelsy Narita, Choirunnisa Miftahul Jannah, L. Ambarsari","doi":"10.29244/cb.8.2.1","DOIUrl":"https://doi.org/10.29244/cb.8.2.1","url":null,"abstract":"According to reports, mango peel contains bioactive compounds—especially phenolic—threefold higher than the flesh. This issue underlies the research that aims to identify bioactive compounds from Indramayu mango peel which have the potential as ACE2 inhibitors. The study was initiated by extracting mango peel simplicia using aquadest, then carried out with phytochemical screening, and identification of compound content by LC-MS. The extraction obtained 21.3% of yield. The results of phytochemical screening showed that secondary metabolites in the form of alkaloids, flavonoids, saponins, and tannins were identified in the mango peel. At the same time, the LC-MS fragmentation results obtained 40 compounds based on the largest sample area. The result from fragmentation were tested for Lipinski bioavailability prediction and ADMET test and obtained 10 potential compounds. Molecular docking was performed on the ACE2 receptor with 10 sample ligands and 1 comparison ligand using YASARA Structure. All of the sample ligands (from Lipinski and ADMET test) showed higher free energies than comparison ligand, chloroquine. Mangiferin is a typical mango compound identified in LC-MS, had inhibitor activity against ACE2. Two-dimensional visualization using Discovery Studio showed mangiferin interaction with the receptor via hydrogen bond, hydrogen carbon bond and phi-anion interactions at three amino acid residues (Thr371, Glu375, Glu402) on the active site of ACE2. \u0000Keywords: ACE2, bioactive compound, mango peel, mangiferin, waste","PeriodicalId":90150,"journal":{"name":"Current cellular biochemistry","volume":"104 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87707067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Review: Gallotannins; Biosynthesis, Structure Activity Relationship, Anti-inflammatory and Antibacterial Activity 点评:鞣花鞣质;生物合成，构效关系，抗炎和抗菌活性

Current cellular biochemistry Pub Date : 2021-06-30 DOI: 10.29244/cb.8.1.1

I. Kurniawan, Hafizh Zahra

{"title":"Review: Gallotannins; Biosynthesis, Structure Activity Relationship, Anti-inflammatory and Antibacterial Activity","authors":"I. Kurniawan, Hafizh Zahra","doi":"10.29244/cb.8.1.1","DOIUrl":"https://doi.org/10.29244/cb.8.1.1","url":null,"abstract":"Tannins are one of the main compounds in plants with potential health benefits. Gallotannin is one of the biologically active tannins groups produced by some medicinal plants. Gallotannins is a hydrolyzable tannin compound biosynthesized through gallic acid, shikimic acid, and pentagalloylglucose pathways. Gallotannins can be separated by cascade solvent extraction procedures, column chromatography, and preparative HPLC. This review focuses on the discussion of biosynthesis and structure-activity relationship of tannins as antibacterial and anti-inflammatory. The structure-activity relationship (SAR) and biological activity of gallotannins is caused by the molecular bonds between gallotannins and certain other compounds, especially proteins, which make complex compounds, change physiological and morphological processes in bacterial cells or tissues. The biological activities of gallotannin include anti-inflammatory, anticancer or antitumour, antiviral, antioxidant, antibacterial, and antidiabetic. This review used the Preferred Reporting Items for Systematic Review and Meta-Analyses (PRISMA) method approach in a short, concise, and clear summary about tannins through various references. \u0000Keywords: Biological activities, Biosynthesis, Gallotannins","PeriodicalId":90150,"journal":{"name":"Current cellular biochemistry","volume":"73 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89509701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Minimum Concentration of Stingless Bee Propolis (Trigona sp.) in Inhibiting the Growth of Dandruff Bacteria 无刺蜂蜂胶(Trigona sp.)抑制头皮屑细菌生长的最低浓度

Current cellular biochemistry Pub Date : 2021-06-30 DOI: 10.29244/cb.8.1.2

Akhmad Endang, Zainal Hasan, Made Artika, Erlank Bagjavicenna

{"title":"Minimum Concentration of Stingless Bee Propolis (Trigona sp.) in Inhibiting the Growth of Dandruff Bacteria","authors":"Akhmad Endang, Zainal Hasan, Made Artika, Erlank Bagjavicenna","doi":"10.29244/cb.8.1.2","DOIUrl":"https://doi.org/10.29244/cb.8.1.2","url":null,"abstract":"Lack of confidence in men and women, one of which is when there is dandruff on the hair and scalp. Hair loss is a further symptom of the presence of white scales on the scalp that cause itching. The presence of excessive microorganisms can aggravate this dandruff. Ingredients such as ketocanozole, zinc pyrithione, selenium sulfide and sulfur are compounds commonly used in shampoo formulas. Propolis is an alternative natural ingredient to treat dandruff. The purpose of this study was to determine the minimum growth inhibitory concentration (MIC) of propolis against dandruff-causing bacteria. Dandruff-causing microbes were isolated from dandruff hair and with the well method in a petri dish, the minimum growth inhibitory concentration of propolis was determined. The microbial colonies produced were very diverse with a round shape like grapes, yellowish white in color. The concentration of 6.25% is the MIC value of propolis extract against dandruff microbes. The effectiveness values against ketoconazole and propolis X from the tested propolis extract were 223.52% and 99.59%, respectively. \u0000 \u0000Keywords: hair loss, dandruff, propolis, bacteria , Trigona","PeriodicalId":90150,"journal":{"name":"Current cellular biochemistry","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83736570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antioxidant Activity, Inhibition α-Glucosidase of Cymbopogon nardus (L.) Rendle and Identification of Active Compounds Cymbopogon nardus (L.)抗氧化活性及α-葡萄糖苷酶抑制活性化合物的研究与鉴定

Current cellular biochemistry Pub Date : 2021-06-30 DOI: 10.29244/cb.8.1.3

Najmah Najmah, H. Hasim, D. Faridah

{"title":"Antioxidant Activity, Inhibition α-Glucosidase of Cymbopogon nardus (L.) Rendle and Identification of Active Compounds","authors":"Najmah Najmah, H. Hasim, D. Faridah","doi":"10.29244/cb.8.1.3","DOIUrl":"https://doi.org/10.29244/cb.8.1.3","url":null,"abstract":"Diabetes mellitus (DM) has become a health problem in various countries, even in Indonesia, multiple of Covid-19 sufferers have DM as comorbid. Treatment of DM can be conducted by delaying glucose intake performed by the α-glucosidase enzyme in the intestine. Treatment using synthetic drugs often causes side effects. Instead to overcome these problems, herbal medicine is a choice. Cymbopogon nardus (L.) Rendle served as a potential candidate as seen from prior research but related to DM drugs, further exploration needs to be performed. This study aims to acquire a DM medicine by using Cymbopogon nardus (L.) Rendle . The leaves were macerated using 96% concentration of ethanol followed by multilevel fractionation with n-hexane, water and ethyl acetate as solvents. The test results showed that the n-hexane fraction was the best for antioxidant activity and α -glucosidase inhibition with IC50 values of 8,23 ppm and 311,87 ppm while the identification of the compound by using Liquid Chromatograph-Mass Spectrometry (LC-MS/MS) suspected compounds that have a role were ar-turmerone, beesioside N and notohamosin A. Based on the test, the n-hexane fraction was found as a source of antioxidants as well as α -glucosidase inhibitors. \u0000 \u0000Keywords: Antioxidant, α -glucosidase, Cymbopogon nardus (L.) Rendle, LC-MS/MS.","PeriodicalId":90150,"journal":{"name":"Current cellular biochemistry","volume":"69 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80282032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Antioxidant Activities and α-glucosidase Inhibition of Gayo Arabica Coffee Skin (Coffea arabica L) 阿拉比卡咖啡皮的抗氧化活性及α-葡萄糖苷酶抑制作用

Current cellular biochemistry Pub Date : 2021-06-30 DOI: 10.29244/cb.8.1.4

Baharuddin Yusuf Habiby Harahap, H. Hasim, D. Faridah

{"title":"Antioxidant Activities and α-glucosidase Inhibition of Gayo Arabica Coffee Skin (Coffea arabica L)","authors":"Baharuddin Yusuf Habiby Harahap, H. Hasim, D. Faridah","doi":"10.29244/cb.8.1.4","DOIUrl":"https://doi.org/10.29244/cb.8.1.4","url":null,"abstract":"This research objectives to determine antioxidant activities and inhibition of α-glucosidase 96 % ethanol extract of gayo arabica coffee skin, n-hexane fraction, ethyl acetate fraction, and water fraction. Gayo arabica coffee skin was extracted by ethanol using maceration and fractionation using n-hexane, ethyl acetate, and water. Quercetin and Gallic acid were used as standard to found total flavonoids and phenolics. Test of antioxidant activity using the DPPH method. After being tested the inhibition of the α-glucosidase IC50 ethanol extract, the n-hexane fraction, ethyl acetate fraction, and water fractions. The total flavonoids of 96% ethanol extract, n-hexane fraction, ethyl acetate, and water fraction respectively are 76.74, 99.07, 73.55, 50.24 mg QE/g extract . Total phenolics of 96% ethanol extract, n-hexane fraction, ethyl acetate, and water fraction respectively are respectively 211.85, 136.67, 145.19, 127.41 mg GAE/ g extract. Antioxidants activity of ethanol extract, n-hexane fraction, ethyl acetate fraction, and water fraction are IC50 12.03 ppm, 36.08 ppm, 30.27 ppm, 11.62 ppm. Inhibition activity α-glucosidase enzyme respectively are 588.85 ppm, 341.81 ppm, 591.61 ppm, and 798.78 ppm. These showed that the ethanol extract and water fraction are the best in antioxidants and the ethanol extract and ethyl acetate fraction are the best in inhibiting the α-glucosidase enzyme. These results indicate that gayo arabica coffee skin extract has the potential to be developed as an antidiabetic compound. \u0000Keywords: Antidiabetic, Antioxidant, flavonoids, phenolics, α-glucosidase","PeriodicalId":90150,"journal":{"name":"Current cellular biochemistry","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90084030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Potency of Senggani (Melastoma malabathricum L) Leaves in Repair of Pancreatic Beta Cells for Diabetes Mellitus Patients: A Narrative Review 桑加尼(malabathicum L)叶对糖尿病患者胰腺β细胞修复作用的研究进展

Current cellular biochemistry Pub Date : 2020-12-21 DOI: 10.29244/CB.7.2.3

G. Prayoga, Aziz Syamsul Huda, Syndilona Br Sitepu

{"title":"The Potency of Senggani (Melastoma malabathricum L) Leaves in Repair of Pancreatic Beta Cells for Diabetes Mellitus Patients: A Narrative Review","authors":"G. Prayoga, Aziz Syamsul Huda, Syndilona Br Sitepu","doi":"10.29244/CB.7.2.3","DOIUrl":"https://doi.org/10.29244/CB.7.2.3","url":null,"abstract":"Diabetes mellitus is often caused by damage to pancreatic beta cells which play a role in secreting insulin in the body. Damage to pancreatic beta cells causes the body to lack insulin. Dipeptidyl peptidase-4 (DPP-4) is a peptidase enzyme complex located on the surface of the cell membrane. Inhibition of the DPP4 enzyme will increase blood GLP-1 levels and induce regeneration of pancreatic beta cells. Senggani leaf (Melastoma malabathricum) boiled water is believed by the people of the Ciamis area to be used as a diabetes medicine. There have been many studies and reviews related to Senggani (Melastoma malabathricum) and its potential. This review focuses on the discussion of Senggani as an antidiabetic by analyzing the reduction in glucose levels and the repair ability of pancreatic beta cells. The results of the literature study that show that senggani leaves have the ability to reduce blood glucose levels and repair activity of pancreatic beta cells through the DPP-4 enzyme inhibition mechanism supported by molecular docking simulation data. There are 12 active compounds that have a binding site similarity above 50% with the comparison compound vildagliptin. Rutin is the best active compound which has a 100% similarity of the binding site. Based on in vivo research and toxicity analysis on the admetsar database, senggani leaf extract and active compounds of senggani leaves have low toxicity, making it safe to be used as antidiabetic herbal preparations.","PeriodicalId":90150,"journal":{"name":"Current cellular biochemistry","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84682744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

The Spray of Pegagan Leaf Extract as an Antifungal of Vulvovaginal Candidiasis: A Narrative Review 山茱萸叶提取物喷雾抗外阴阴道念珠菌病的研究进展

Current cellular biochemistry Pub Date : 2020-12-21 DOI: 10.29244/CB.7.2.5

Santi Nuraeni, S. Rahmadanti, Aldi Fadilah, Husnawati

引用次数: 2